Genotyping of Polymorphisms in Alcohol and Aldehyde Dehydrogenase Genes by Direct Application of PCR-RFLP on Dried Blood without DNA Extraction

We have developed a simple, labor-saving, inexpensive, and rapid single nucleotide polymorphism (SNP) genotyping method that works directly on whole human blood. This single-tube genotyping method was used to successfully and reliably genotype ADH1B and ALDH2 polymorphisms without DNA isolation usin...

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Veröffentlicht in:	Analytical Sciences 2010/04/10, Vol.26(4), pp.503-505
Hauptverfasser:	HAYASHIDA, Mariko, IWAO-KOIZUMI, Kyoko, MURATA, Shigenori, YOKOYAMA, Akira, KINOSHITA, Kenji
Format:	Artikel
Sprache:	eng
Schlagworte:	Alcohol Dehydrogenase - genetics Aldehyde Dehydrogenase - genetics Aldehyde Dehydrogenase, Mitochondrial Analytical Chemistry Blood Chemistry DNA - isolation & purification Genotype Humans Polymerase Chain Reaction - economics Polymerase Chain Reaction - methods Polymorphism, Genetic Polymorphism, Restriction Fragment Length Time Factors
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