Identification of Vibrio harveyi as a causative bacterium for a tail rot disease of sea bream Sparus aurata from research hatchery in Malta
A bacterial disease was reported from gilthead sea bream ( Sparus aurata) within a hatchery environment in Malta. Symptoms included complete erosion of tail, infection in the eye, mucous secretion and frequent mortality. A total of 540 strains were initially isolated in marine agar from different in...
Gespeichert in:
| Veröffentlicht in: | Microbiological research 2010-10, Vol.165 (8), p.639-648 |
|---|---|
| Hauptverfasser: | , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 648 |
|---|---|
| container_issue | 8 |
| container_start_page | 639 |
| container_title | Microbiological research |
| container_volume | 165 |
| creator | Haldar, S. Maharajan, A. Chatterjee, S. Hunter, S.A. Chowdhury, N. Hinenoya, A. Asakura, M. Yamasaki, S. |
| description | A bacterial disease was reported from gilthead sea bream (
Sparus aurata) within a hatchery environment in Malta. Symptoms included complete erosion of tail, infection in the eye, mucous secretion and frequent mortality. A total of 540 strains were initially isolated in marine agar from different infected body parts and culture water sources. Subsequently 100 isolates were randomly selected, identified biochemically and all were found to be
Vibrio harveyi-related organisms; finally from 100 isolates a total of 13 numbers were randomly selected and accurately identified as
V. harveyi by 16S rRNA gene sequencing and species-specific PCR. Ribotyping of these strains with
HindIII revealed total of six clusters.
In vivo challenge study with representative isolates from each cluster proved two clusters each were highly pathogenic, moderately pathogenic and non-pathogenic. All 13 isolates were positive for hemolysin gene, a potential virulence factor. Further analysis revealed probably a single copy of this gene was encoded in all isolates, although not in the same locus in the genome. Although
V. harveyi was reported to be an important pathogen for many aquatic organisms, to our knowledge this might be the first report of disease caused by
V. harveyi and their systematic study in the sea bream hatchery from Malta. |
| doi_str_mv | 10.1016/j.micres.2009.12.001 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_817606590</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0944501309001177</els_id><sourcerecordid>762469913</sourcerecordid><originalsourceid>FETCH-LOGICAL-c459t-bc2f19711c5ab4617cce56799e4968a9be133f73794ace59ceebddd9392e8fc33</originalsourceid><addsrcrecordid>eNqFkcFu1DAQhi0EokvhDRDyjVOC7SROfEFCFS2VijjQcrUmzkTrVRIvY2elfQZeGq-29EhPtjTf_L_lj7H3UpRSSP1pV87eEcZSCWFKqUoh5Au2kVp2hdCqesk2wtR10QhZXbA3Me4yUJtOvWYXSkhlWt1s2J_bAZfkR-8g-bDwMPJfvicf-BbogEfPIXLgDtaYgQPyHlxC8uvMx0B5ksBPnELig48IEU8J-cJ7Qpj5zz3QmgNWggR8pDDz_GQEcttckNwW6cj9wr_DlOAtezXCFPHd43nJHq6_3l99K-5-3NxefbkrXN2YVPROjdK0UroG-lrL1jlsdGsM1kZ3YHqUVTW2VWtqyBPjEPthGExlFHajq6pL9vGcu6fwe8WY7Oyjw2mCBcMabSdbLXRjxLNkq1WtjZGnzPpMOgoxEo52T34GOlop7MmX3dmzL3vyZaWyWUde-_BYsPYzDk9L_wRl4PMZwPwhB49ko_O4OBw8oUt2CP7_DX8BRXSqkA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>762469913</pqid></control><display><type>article</type><title>Identification of Vibrio harveyi as a causative bacterium for a tail rot disease of sea bream Sparus aurata from research hatchery in Malta</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals Complete</source><source>EZB-FREE-00999 freely available EZB journals</source><creator>Haldar, S. ; Maharajan, A. ; Chatterjee, S. ; Hunter, S.A. ; Chowdhury, N. ; Hinenoya, A. ; Asakura, M. ; Yamasaki, S.</creator><creatorcontrib>Haldar, S. ; Maharajan, A. ; Chatterjee, S. ; Hunter, S.A. ; Chowdhury, N. ; Hinenoya, A. ; Asakura, M. ; Yamasaki, S.</creatorcontrib><description>A bacterial disease was reported from gilthead sea bream (
Sparus aurata) within a hatchery environment in Malta. Symptoms included complete erosion of tail, infection in the eye, mucous secretion and frequent mortality. A total of 540 strains were initially isolated in marine agar from different infected body parts and culture water sources. Subsequently 100 isolates were randomly selected, identified biochemically and all were found to be
Vibrio harveyi-related organisms; finally from 100 isolates a total of 13 numbers were randomly selected and accurately identified as
V. harveyi by 16S rRNA gene sequencing and species-specific PCR. Ribotyping of these strains with
HindIII revealed total of six clusters.
In vivo challenge study with representative isolates from each cluster proved two clusters each were highly pathogenic, moderately pathogenic and non-pathogenic. All 13 isolates were positive for hemolysin gene, a potential virulence factor. Further analysis revealed probably a single copy of this gene was encoded in all isolates, although not in the same locus in the genome. Although
V. harveyi was reported to be an important pathogen for many aquatic organisms, to our knowledge this might be the first report of disease caused by
V. harveyi and their systematic study in the sea bream hatchery from Malta.</description><identifier>ISSN: 0944-5013</identifier><identifier>EISSN: 1618-0623</identifier><identifier>DOI: 10.1016/j.micres.2009.12.001</identifier><identifier>PMID: 20129765</identifier><language>eng</language><publisher>Germany: Elsevier GmbH</publisher><subject>Animals ; Aquaculture ; Bacterial Proteins - genetics ; Bacterial Typing Techniques ; Cluster Analysis ; DNA, Bacterial - chemistry ; DNA, Bacterial - genetics ; DNA, Ribosomal - chemistry ; DNA, Ribosomal - genetics ; Exudates and Transudates ; Eye - pathology ; Fish Diseases - microbiology ; Fish Diseases - mortality ; Fish Diseases - pathology ; Hemolysin ; Hemolysin Proteins - genetics ; Malta ; Marine ; Molecular Sequence Data ; Phylogeny ; RNA, Ribosomal, 16S - genetics ; Sea Bream - microbiology ; Sea bream hatchery ; Sequence Analysis, DNA ; Sparus aurata ; Tail - pathology ; Tail rot ; Vibrio ; Vibrio - classification ; Vibrio - genetics ; Vibrio - isolation & purification ; Vibrio harveyi ; Vibrio Infections - microbiology ; Vibrio Infections - mortality ; Vibrio Infections - pathology ; Vibrio Infections - veterinary ; Virulence Factors - genetics</subject><ispartof>Microbiological research, 2010-10, Vol.165 (8), p.639-648</ispartof><rights>2009 Elsevier GmbH</rights><rights>Copyright © 2009 Elsevier GmbH. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c459t-bc2f19711c5ab4617cce56799e4968a9be133f73794ace59ceebddd9392e8fc33</citedby><cites>FETCH-LOGICAL-c459t-bc2f19711c5ab4617cce56799e4968a9be133f73794ace59ceebddd9392e8fc33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.micres.2009.12.001$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20129765$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Haldar, S.</creatorcontrib><creatorcontrib>Maharajan, A.</creatorcontrib><creatorcontrib>Chatterjee, S.</creatorcontrib><creatorcontrib>Hunter, S.A.</creatorcontrib><creatorcontrib>Chowdhury, N.</creatorcontrib><creatorcontrib>Hinenoya, A.</creatorcontrib><creatorcontrib>Asakura, M.</creatorcontrib><creatorcontrib>Yamasaki, S.</creatorcontrib><title>Identification of Vibrio harveyi as a causative bacterium for a tail rot disease of sea bream Sparus aurata from research hatchery in Malta</title><title>Microbiological research</title><addtitle>Microbiol Res</addtitle><description>A bacterial disease was reported from gilthead sea bream (
Sparus aurata) within a hatchery environment in Malta. Symptoms included complete erosion of tail, infection in the eye, mucous secretion and frequent mortality. A total of 540 strains were initially isolated in marine agar from different infected body parts and culture water sources. Subsequently 100 isolates were randomly selected, identified biochemically and all were found to be
Vibrio harveyi-related organisms; finally from 100 isolates a total of 13 numbers were randomly selected and accurately identified as
V. harveyi by 16S rRNA gene sequencing and species-specific PCR. Ribotyping of these strains with
HindIII revealed total of six clusters.
In vivo challenge study with representative isolates from each cluster proved two clusters each were highly pathogenic, moderately pathogenic and non-pathogenic. All 13 isolates were positive for hemolysin gene, a potential virulence factor. Further analysis revealed probably a single copy of this gene was encoded in all isolates, although not in the same locus in the genome. Although
V. harveyi was reported to be an important pathogen for many aquatic organisms, to our knowledge this might be the first report of disease caused by
V. harveyi and their systematic study in the sea bream hatchery from Malta.</description><subject>Animals</subject><subject>Aquaculture</subject><subject>Bacterial Proteins - genetics</subject><subject>Bacterial Typing Techniques</subject><subject>Cluster Analysis</subject><subject>DNA, Bacterial - chemistry</subject><subject>DNA, Bacterial - genetics</subject><subject>DNA, Ribosomal - chemistry</subject><subject>DNA, Ribosomal - genetics</subject><subject>Exudates and Transudates</subject><subject>Eye - pathology</subject><subject>Fish Diseases - microbiology</subject><subject>Fish Diseases - mortality</subject><subject>Fish Diseases - pathology</subject><subject>Hemolysin</subject><subject>Hemolysin Proteins - genetics</subject><subject>Malta</subject><subject>Marine</subject><subject>Molecular Sequence Data</subject><subject>Phylogeny</subject><subject>RNA, Ribosomal, 16S - genetics</subject><subject>Sea Bream - microbiology</subject><subject>Sea bream hatchery</subject><subject>Sequence Analysis, DNA</subject><subject>Sparus aurata</subject><subject>Tail - pathology</subject><subject>Tail rot</subject><subject>Vibrio</subject><subject>Vibrio - classification</subject><subject>Vibrio - genetics</subject><subject>Vibrio - isolation & purification</subject><subject>Vibrio harveyi</subject><subject>Vibrio Infections - microbiology</subject><subject>Vibrio Infections - mortality</subject><subject>Vibrio Infections - pathology</subject><subject>Vibrio Infections - veterinary</subject><subject>Virulence Factors - genetics</subject><issn>0944-5013</issn><issn>1618-0623</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkcFu1DAQhi0EokvhDRDyjVOC7SROfEFCFS2VijjQcrUmzkTrVRIvY2elfQZeGq-29EhPtjTf_L_lj7H3UpRSSP1pV87eEcZSCWFKqUoh5Au2kVp2hdCqesk2wtR10QhZXbA3Me4yUJtOvWYXSkhlWt1s2J_bAZfkR-8g-bDwMPJfvicf-BbogEfPIXLgDtaYgQPyHlxC8uvMx0B5ksBPnELig48IEU8J-cJ7Qpj5zz3QmgNWggR8pDDz_GQEcttckNwW6cj9wr_DlOAtezXCFPHd43nJHq6_3l99K-5-3NxefbkrXN2YVPROjdK0UroG-lrL1jlsdGsM1kZ3YHqUVTW2VWtqyBPjEPthGExlFHajq6pL9vGcu6fwe8WY7Oyjw2mCBcMabSdbLXRjxLNkq1WtjZGnzPpMOgoxEo52T34GOlop7MmX3dmzL3vyZaWyWUde-_BYsPYzDk9L_wRl4PMZwPwhB49ko_O4OBw8oUt2CP7_DX8BRXSqkA</recordid><startdate>20101020</startdate><enddate>20101020</enddate><creator>Haldar, S.</creator><creator>Maharajan, A.</creator><creator>Chatterjee, S.</creator><creator>Hunter, S.A.</creator><creator>Chowdhury, N.</creator><creator>Hinenoya, A.</creator><creator>Asakura, M.</creator><creator>Yamasaki, S.</creator><general>Elsevier GmbH</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QL</scope><scope>7TN</scope><scope>C1K</scope><scope>F1W</scope><scope>H95</scope><scope>H98</scope><scope>L.G</scope></search><sort><creationdate>20101020</creationdate><title>Identification of Vibrio harveyi as a causative bacterium for a tail rot disease of sea bream Sparus aurata from research hatchery in Malta</title><author>Haldar, S. ; Maharajan, A. ; Chatterjee, S. ; Hunter, S.A. ; Chowdhury, N. ; Hinenoya, A. ; Asakura, M. ; Yamasaki, S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c459t-bc2f19711c5ab4617cce56799e4968a9be133f73794ace59ceebddd9392e8fc33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Animals</topic><topic>Aquaculture</topic><topic>Bacterial Proteins - genetics</topic><topic>Bacterial Typing Techniques</topic><topic>Cluster Analysis</topic><topic>DNA, Bacterial - chemistry</topic><topic>DNA, Bacterial - genetics</topic><topic>DNA, Ribosomal - chemistry</topic><topic>DNA, Ribosomal - genetics</topic><topic>Exudates and Transudates</topic><topic>Eye - pathology</topic><topic>Fish Diseases - microbiology</topic><topic>Fish Diseases - mortality</topic><topic>Fish Diseases - pathology</topic><topic>Hemolysin</topic><topic>Hemolysin Proteins - genetics</topic><topic>Malta</topic><topic>Marine</topic><topic>Molecular Sequence Data</topic><topic>Phylogeny</topic><topic>RNA, Ribosomal, 16S - genetics</topic><topic>Sea Bream - microbiology</topic><topic>Sea bream hatchery</topic><topic>Sequence Analysis, DNA</topic><topic>Sparus aurata</topic><topic>Tail - pathology</topic><topic>Tail rot</topic><topic>Vibrio</topic><topic>Vibrio - classification</topic><topic>Vibrio - genetics</topic><topic>Vibrio - isolation & purification</topic><topic>Vibrio harveyi</topic><topic>Vibrio Infections - microbiology</topic><topic>Vibrio Infections - mortality</topic><topic>Vibrio Infections - pathology</topic><topic>Vibrio Infections - veterinary</topic><topic>Virulence Factors - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Haldar, S.</creatorcontrib><creatorcontrib>Maharajan, A.</creatorcontrib><creatorcontrib>Chatterjee, S.</creatorcontrib><creatorcontrib>Hunter, S.A.</creatorcontrib><creatorcontrib>Chowdhury, N.</creatorcontrib><creatorcontrib>Hinenoya, A.</creatorcontrib><creatorcontrib>Asakura, M.</creatorcontrib><creatorcontrib>Yamasaki, S.</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Oceanic Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Aquaculture Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><jtitle>Microbiological research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Haldar, S.</au><au>Maharajan, A.</au><au>Chatterjee, S.</au><au>Hunter, S.A.</au><au>Chowdhury, N.</au><au>Hinenoya, A.</au><au>Asakura, M.</au><au>Yamasaki, S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification of Vibrio harveyi as a causative bacterium for a tail rot disease of sea bream Sparus aurata from research hatchery in Malta</atitle><jtitle>Microbiological research</jtitle><addtitle>Microbiol Res</addtitle><date>2010-10-20</date><risdate>2010</risdate><volume>165</volume><issue>8</issue><spage>639</spage><epage>648</epage><pages>639-648</pages><issn>0944-5013</issn><eissn>1618-0623</eissn><abstract>A bacterial disease was reported from gilthead sea bream (
Sparus aurata) within a hatchery environment in Malta. Symptoms included complete erosion of tail, infection in the eye, mucous secretion and frequent mortality. A total of 540 strains were initially isolated in marine agar from different infected body parts and culture water sources. Subsequently 100 isolates were randomly selected, identified biochemically and all were found to be
Vibrio harveyi-related organisms; finally from 100 isolates a total of 13 numbers were randomly selected and accurately identified as
V. harveyi by 16S rRNA gene sequencing and species-specific PCR. Ribotyping of these strains with
HindIII revealed total of six clusters.
In vivo challenge study with representative isolates from each cluster proved two clusters each were highly pathogenic, moderately pathogenic and non-pathogenic. All 13 isolates were positive for hemolysin gene, a potential virulence factor. Further analysis revealed probably a single copy of this gene was encoded in all isolates, although not in the same locus in the genome. Although
V. harveyi was reported to be an important pathogen for many aquatic organisms, to our knowledge this might be the first report of disease caused by
V. harveyi and their systematic study in the sea bream hatchery from Malta.</abstract><cop>Germany</cop><pub>Elsevier GmbH</pub><pmid>20129765</pmid><doi>10.1016/j.micres.2009.12.001</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0944-5013 |
| ispartof | Microbiological research, 2010-10, Vol.165 (8), p.639-648 |
| issn | 0944-5013 1618-0623 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_817606590 |
| source | MEDLINE; Elsevier ScienceDirect Journals Complete; EZB-FREE-00999 freely available EZB journals |
| subjects | Animals Aquaculture Bacterial Proteins - genetics Bacterial Typing Techniques Cluster Analysis DNA, Bacterial - chemistry DNA, Bacterial - genetics DNA, Ribosomal - chemistry DNA, Ribosomal - genetics Exudates and Transudates Eye - pathology Fish Diseases - microbiology Fish Diseases - mortality Fish Diseases - pathology Hemolysin Hemolysin Proteins - genetics Malta Marine Molecular Sequence Data Phylogeny RNA, Ribosomal, 16S - genetics Sea Bream - microbiology Sea bream hatchery Sequence Analysis, DNA Sparus aurata Tail - pathology Tail rot Vibrio Vibrio - classification Vibrio - genetics Vibrio - isolation & purification Vibrio harveyi Vibrio Infections - microbiology Vibrio Infections - mortality Vibrio Infections - pathology Vibrio Infections - veterinary Virulence Factors - genetics |
| title | Identification of Vibrio harveyi as a causative bacterium for a tail rot disease of sea bream Sparus aurata from research hatchery in Malta |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-25T11%3A50%3A07IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Identification%20of%20Vibrio%20harveyi%20as%20a%20causative%20bacterium%20for%20a%20tail%20rot%20disease%20of%20sea%20bream%20Sparus%20aurata%20from%20research%20hatchery%20in%20Malta&rft.jtitle=Microbiological%20research&rft.au=Haldar,%20S.&rft.date=2010-10-20&rft.volume=165&rft.issue=8&rft.spage=639&rft.epage=648&rft.pages=639-648&rft.issn=0944-5013&rft.eissn=1618-0623&rft_id=info:doi/10.1016/j.micres.2009.12.001&rft_dat=%3Cproquest_cross%3E762469913%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=762469913&rft_id=info:pmid/20129765&rft_els_id=S0944501309001177&rfr_iscdi=true |