Alanine dehydrogenase from the psychrophilic bacterium strain PA-43: Overexpression, molecular characterization, and sequence analysis

The gene encoding alanine dehydrogenase (AlaDH; EC 1.4.1.1) from the marine psychrophilic bacterium strain PA-43 was cloned, sequenced, and overexpressed in Escherichia coli. The primary structure was deduced on the basis of the nucleotide sequence. The enzyme subunit contains 371 amino acid residue...

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Veröffentlicht in:	Extremophiles : life under extreme conditions 2003-04, Vol.7 (2), p.135-143
Hauptverfasser:	IRWIN, Jane A, LYNCH, Susan V, COUGHLAN, Suzie, BAKER, Patrick J, GUDMUNDSSON, Haflidi M, ALFREDSSON, Gudni A, RICE, David W, ENGEL, Paul C
Format:	Artikel
Sprache:	eng
Schlagworte:	Acclimatization Alanine Dehydrogenase Amino Acid Oxidoreductases - chemistry Amino Acid Oxidoreductases - genetics Amino Acid Oxidoreductases - metabolism Amino Acid Sequence Amino acids Bacteriology Base Sequence Biological and medical sciences Biotechnology Cloning Cloning, Molecular Cold Temperature Dehydrogenase DNA, Bacterial - genetics E coli Enzyme Activation - drug effects Enzyme engineering Enzyme Inhibitors - pharmacology Enzymes Escherichia coli Escherichia coli - genetics Fundamental and applied biological sciences. Psychology Gene expression Genes, Bacterial Genetics Gram-Negative Bacteria - enzymology Gram-Negative Bacteria - genetics Kinetics Methods. Procedures. Technologies Microbiology Miscellaneous Molecular biology Molecular Sequence Data Phormidium Recombinant Proteins - chemistry Recombinant Proteins - genetics Recombinant Proteins - metabolism Sequence Homology, Amino Acid Shewanella Space life sciences Substrate Specificity Thermodynamics Vibrio proteolyticus
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