The ionic basis of the membrane potential in the early Asterias embryo
The ionic basis of the resting potential in the fertilized Asterias embryo has been investigated. The response of the membrane potential (as determined by intracellular microelectrodes) to changes in external K concentration was stable and reversible up to 200 m M K. The time course and magnitude of...
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| Veröffentlicht in: | Developmental biology 1972-11, Vol.29 (3), p.273-282 |
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| container_title | Developmental biology |
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| creator | Tupper, Joseph T. |
| description | The ionic basis of the resting potential in the fertilized
Asterias embryo has been investigated. The response of the membrane potential (as determined by intracellular microelectrodes) to changes in external K concentration was stable and reversible up to 200 m
M K. The time course and magnitude of the response observed upon changes in external K is essentially independent of the concentration of external Cl. There is a significant effect of Na ion on the membrane potential, especially at low external K.
The response of the membrane potential to changes in external K could be predicted by a form of the constant field equation. Plots of
e
VF
RT
versus external K, in conjunction with the constant field equation, allow an estimate of the internal K concentration and the ratio of Na to K permeability (
P
Na
P
K
). These plots indicate at this stage of development an internal K concentration of approximately 200 m
M and a
P
Na
P
K
of 0.03. |
| doi_str_mv | 10.1016/0012-1606(72)90067-X |
| format | Article |
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Asterias embryo has been investigated. The response of the membrane potential (as determined by intracellular microelectrodes) to changes in external K concentration was stable and reversible up to 200 m
M K. The time course and magnitude of the response observed upon changes in external K is essentially independent of the concentration of external Cl. There is a significant effect of Na ion on the membrane potential, especially at low external K.
The response of the membrane potential to changes in external K could be predicted by a form of the constant field equation. Plots of
e
VF
RT
versus external K, in conjunction with the constant field equation, allow an estimate of the internal K concentration and the ratio of Na to K permeability (
P
Na
P
K
). These plots indicate at this stage of development an internal K concentration of approximately 200 m
M and a
P
Na
P
K
of 0.03.</description><identifier>ISSN: 0012-1606</identifier><identifier>EISSN: 1095-564X</identifier><identifier>DOI: 10.1016/0012-1606(72)90067-X</identifier><identifier>PMID: 4652269</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Chlorides - pharmacology ; Embryo, Nonmammalian - physiology ; Membrane Potentials - drug effects ; Microelectrodes ; Potassium - pharmacology ; Sodium - pharmacology ; Starfish - embryology</subject><ispartof>Developmental biology, 1972-11, Vol.29 (3), p.273-282</ispartof><rights>1972</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c357t-98fc8c020224e1fdf011c8137406e4391a6cdf8d36f7b2825a96c7ba6fd260193</citedby><cites>FETCH-LOGICAL-c357t-98fc8c020224e1fdf011c8137406e4391a6cdf8d36f7b2825a96c7ba6fd260193</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0012-1606(72)90067-X$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,27922,27923,45993</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/4652269$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tupper, Joseph T.</creatorcontrib><title>The ionic basis of the membrane potential in the early Asterias embryo</title><title>Developmental biology</title><addtitle>Dev Biol</addtitle><description>The ionic basis of the resting potential in the fertilized
Asterias embryo has been investigated. The response of the membrane potential (as determined by intracellular microelectrodes) to changes in external K concentration was stable and reversible up to 200 m
M K. The time course and magnitude of the response observed upon changes in external K is essentially independent of the concentration of external Cl. There is a significant effect of Na ion on the membrane potential, especially at low external K.
The response of the membrane potential to changes in external K could be predicted by a form of the constant field equation. Plots of
e
VF
RT
versus external K, in conjunction with the constant field equation, allow an estimate of the internal K concentration and the ratio of Na to K permeability (
P
Na
P
K
). These plots indicate at this stage of development an internal K concentration of approximately 200 m
M and a
P
Na
P
K
of 0.03.</description><subject>Animals</subject><subject>Chlorides - pharmacology</subject><subject>Embryo, Nonmammalian - physiology</subject><subject>Membrane Potentials - drug effects</subject><subject>Microelectrodes</subject><subject>Potassium - pharmacology</subject><subject>Sodium - pharmacology</subject><subject>Starfish - embryology</subject><issn>0012-1606</issn><issn>1095-564X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1972</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kF9LwzAUxYMoc06_gUKeRB-qN2mbtC_CGE6FgS8T9hbS9AYjbTOTTti3t3Njjz5duOec--dHyDWDBwZMPAIwnjAB4k7y-xJAyGR1QsYMyjzJRbY6JeOj5ZxcxPgFAGlRpCMyykTOuSjHZL78ROp85wytdHSRekv7odViWwXdIV37Hrve6Ya67k9BHZotncYeg9OR7nxbf0nOrG4iXh3qhHzMn5ez12Tx_vI2my4Sk-ayT8rCmsIAB84zZLa2wJgpWCozEJilJdPC1LaoU2FlxQue61IYWWlhay6AlemE3O7nroP_3mDsVeuiwaYZTvWbqAomQQieDcZsbzTBxxjQqnVwrQ5bxUDt8KkdG7VjoyRXf_jUaojdHOZvqhbrY-jAa9Cf9joOT_44DCoah53B2gU0vaq9-3_BL4asfeM</recordid><startdate>197211</startdate><enddate>197211</enddate><creator>Tupper, Joseph T.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>197211</creationdate><title>The ionic basis of the membrane potential in the early Asterias embryo</title><author>Tupper, Joseph T.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c357t-98fc8c020224e1fdf011c8137406e4391a6cdf8d36f7b2825a96c7ba6fd260193</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1972</creationdate><topic>Animals</topic><topic>Chlorides - pharmacology</topic><topic>Embryo, Nonmammalian - physiology</topic><topic>Membrane Potentials - drug effects</topic><topic>Microelectrodes</topic><topic>Potassium - pharmacology</topic><topic>Sodium - pharmacology</topic><topic>Starfish - embryology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tupper, Joseph T.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Developmental biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tupper, Joseph T.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The ionic basis of the membrane potential in the early Asterias embryo</atitle><jtitle>Developmental biology</jtitle><addtitle>Dev Biol</addtitle><date>1972-11</date><risdate>1972</risdate><volume>29</volume><issue>3</issue><spage>273</spage><epage>282</epage><pages>273-282</pages><issn>0012-1606</issn><eissn>1095-564X</eissn><abstract>The ionic basis of the resting potential in the fertilized
Asterias embryo has been investigated. The response of the membrane potential (as determined by intracellular microelectrodes) to changes in external K concentration was stable and reversible up to 200 m
M K. The time course and magnitude of the response observed upon changes in external K is essentially independent of the concentration of external Cl. There is a significant effect of Na ion on the membrane potential, especially at low external K.
The response of the membrane potential to changes in external K could be predicted by a form of the constant field equation. Plots of
e
VF
RT
versus external K, in conjunction with the constant field equation, allow an estimate of the internal K concentration and the ratio of Na to K permeability (
P
Na
P
K
). These plots indicate at this stage of development an internal K concentration of approximately 200 m
M and a
P
Na
P
K
of 0.03.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>4652269</pmid><doi>10.1016/0012-1606(72)90067-X</doi><tpages>10</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0012-1606 |
| ispartof | Developmental biology, 1972-11, Vol.29 (3), p.273-282 |
| issn | 0012-1606 1095-564X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_81706624 |
| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Animals Chlorides - pharmacology Embryo, Nonmammalian - physiology Membrane Potentials - drug effects Microelectrodes Potassium - pharmacology Sodium - pharmacology Starfish - embryology |
| title | The ionic basis of the membrane potential in the early Asterias embryo |
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