Isolation of viral RNA using cesium chloride-ethidium bromide equilibrium sedimentation

The effect of ethidium bromide (EB) on the buoyant density of reovirus RNA during equilibrium sedimentation has been investigated. The addition of the dye ethidium bromide was found to reduce the buoyant density of reovirus RNA in a Cs 2SO 4 gradient by a value of 0.13 to 0.15 g/cc, and provided a s...

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Veröffentlicht in:	Biochemical and biophysical research communications 1972-10, Vol.49 (2), p.543-549
Hauptverfasser:	Lancz, Gerald J., Scaletti, Joseph V., McLaren, Leroy C.
Format:	Artikel
Sprache:	eng
Schlagworte:	Centrifugation, Density Gradient Deoxyribonucleases DNA - isolation & purification Ethidium L Cells (Cell Line) - microbiology Microscopy, Electron Reoviridae Ribonucleases RNA, Viral - isolation & purification Virus Replication
Online-Zugang:	Volltext
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