Comparative Studies on the Metabolic Hydrogenation of the Ring A in Testosterone and Its Conjugates by Male Rat Liver in Vitro
Metabolic hydrogenation of the ring A double bond in testosterone and its conjugates were studied by incubating 14C-testosterone, 14C-testosterone 17-N-acetylglucosaminide and 3H-testosterone 17-glucosiduronate with 20000×g or 105000×g supernatant fluids and microsomal fraction of male rat liver hom...
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| Veröffentlicht in: | Chemical & pharmaceutical bulletin 1972/09/25, Vol.20(9), pp.1913-1920 |
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| container_end_page | 1920 |
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| container_issue | 9 |
| container_start_page | 1913 |
| container_title | Chemical & pharmaceutical bulletin |
| container_volume | 20 |
| creator | MATSUI, MICHIO ABE, FUKUO KIMURA, KYOKO OKADA, MASASHI |
| description | Metabolic hydrogenation of the ring A double bond in testosterone and its conjugates were studied by incubating 14C-testosterone, 14C-testosterone 17-N-acetylglucosaminide and 3H-testosterone 17-glucosiduronate with 20000×g or 105000×g supernatant fluids and microsomal fraction of male rat liver homogenate under carbon monoxide atmosphere or in air. It was demonstrated that in contrast to testosterone the conjugates were not good substrates for microsomal 4-5α-hydrogenase as well as for hydroxylases, while they were better substrates for soluble 4-5β-hydrogenase than testosterone. These findings are consistent with the observations on in vivo metabolism of testosterone and its conjugates in man. |
| doi_str_mv | 10.1248/cpb.20.1913 |
| format | Article |
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It was demonstrated that in contrast to testosterone the conjugates were not good substrates for microsomal 4-5α-hydrogenase as well as for hydroxylases, while they were better substrates for soluble 4-5β-hydrogenase than testosterone. 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These findings are consistent with the observations on in vivo metabolism of testosterone and its conjugates in man.</description><subject>Animals</subject><subject>Carbon Isotopes</subject><subject>Chromatography, Thin Layer</subject><subject>Electrophoresis, Paper</subject><subject>Hydrogenation</subject><subject>In Vitro Techniques</subject><subject>Liver - enzymology</subject><subject>Male</subject><subject>Microsomes, Liver - enzymology</subject><subject>Oxidation-Reduction</subject><subject>Rats</subject><subject>Testosterone - metabolism</subject><subject>Tritium</subject><issn>0009-2363</issn><issn>1347-5223</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1972</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkc2LFDEQxYMo6-zqybMQELwsveaj00mOy6y6C7MIunoN1enMbIaeZEzSwlz82007wwpeqgrer14VPITeUHJFWas-2H1_xeqsKX-GFpS3shGM8edoQQjRDeMdf4nOc94SwgSR_AydtV1bEbJAv5dxt4cExf9y-FuZBu8yjgGXR4fvXYE-jt7i28OQ4saFilUtrv_KX33Y4GvsA35wucRcXIrBYQgDvisZL2PYThso1a8_4HsY6wYUvKqH0rz0w5cUX6EXaxize33qF-j7p48Py9tm9eXz3fJ61VghZWk0h44IRYSTfa-JZUTWwphSliqtAagQMHQENBuGXg094SBasraqo8J2kl-g90fffYo_p_qu2fls3ThCcHHKRtGuooxU8N1_4DZOKdTfDG2lVExS0VXq8kjZFHNObm32ye8gHQwlZs7E1EwMq3PNpNJvT55Tv3PDE3sKoeo3R32bC2zckw6peDu62YtqoWY_fSyz7T_5EZJxgf8BAUSeRg</recordid><startdate>19720101</startdate><enddate>19720101</enddate><creator>MATSUI, MICHIO</creator><creator>ABE, FUKUO</creator><creator>KIMURA, KYOKO</creator><creator>OKADA, MASASHI</creator><general>The Pharmaceutical Society of Japan</general><general>Japan Science and Technology Agency</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>19720101</creationdate><title>Comparative Studies on the Metabolic Hydrogenation of the Ring A in Testosterone and Its Conjugates by Male Rat Liver in Vitro</title><author>MATSUI, MICHIO ; ABE, FUKUO ; KIMURA, KYOKO ; OKADA, MASASHI</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c577t-93a605805e7bb90c2070c22288c1899aa155ad60a92ddb8db03a540fc8615c673</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1972</creationdate><topic>Animals</topic><topic>Carbon Isotopes</topic><topic>Chromatography, Thin Layer</topic><topic>Electrophoresis, Paper</topic><topic>Hydrogenation</topic><topic>In Vitro Techniques</topic><topic>Liver - enzymology</topic><topic>Male</topic><topic>Microsomes, Liver - enzymology</topic><topic>Oxidation-Reduction</topic><topic>Rats</topic><topic>Testosterone - metabolism</topic><topic>Tritium</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>MATSUI, MICHIO</creatorcontrib><creatorcontrib>ABE, FUKUO</creatorcontrib><creatorcontrib>KIMURA, KYOKO</creatorcontrib><creatorcontrib>OKADA, MASASHI</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Chemical & pharmaceutical bulletin</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>MATSUI, MICHIO</au><au>ABE, FUKUO</au><au>KIMURA, KYOKO</au><au>OKADA, MASASHI</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparative Studies on the Metabolic Hydrogenation of the Ring A in Testosterone and Its Conjugates by Male Rat Liver in Vitro</atitle><jtitle>Chemical & pharmaceutical bulletin</jtitle><addtitle>Chem. Pharm. Bull.</addtitle><date>1972-01-01</date><risdate>1972</risdate><volume>20</volume><issue>9</issue><spage>1913</spage><epage>1920</epage><pages>1913-1920</pages><issn>0009-2363</issn><eissn>1347-5223</eissn><abstract>Metabolic hydrogenation of the ring A double bond in testosterone and its conjugates were studied by incubating 14C-testosterone, 14C-testosterone 17-N-acetylglucosaminide and 3H-testosterone 17-glucosiduronate with 20000×g or 105000×g supernatant fluids and microsomal fraction of male rat liver homogenate under carbon monoxide atmosphere or in air. It was demonstrated that in contrast to testosterone the conjugates were not good substrates for microsomal 4-5α-hydrogenase as well as for hydroxylases, while they were better substrates for soluble 4-5β-hydrogenase than testosterone. 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| identifier | ISSN: 0009-2363 |
| ispartof | Chemical and Pharmaceutical Bulletin, 1972/09/25, Vol.20(9), pp.1913-1920 |
| issn | 0009-2363 1347-5223 |
| language | eng |
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| source | J-STAGE Free; MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Free Full-Text Journals in Chemistry |
| subjects | Animals Carbon Isotopes Chromatography, Thin Layer Electrophoresis, Paper Hydrogenation In Vitro Techniques Liver - enzymology Male Microsomes, Liver - enzymology Oxidation-Reduction Rats Testosterone - metabolism Tritium |
| title | Comparative Studies on the Metabolic Hydrogenation of the Ring A in Testosterone and Its Conjugates by Male Rat Liver in Vitro |
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