Human leukemic cells: Fractionation and characterization of ribonucleic acids

RNA extracted from log-phase human lymphocytic cells in large-scale cultures was purified and subjected to long (2.5 × 300 cm) exclusion chromatography (Sephadex G-100 columns) by a novel method which provided greater separation of the four main fractions, as well as separation of lower molecular we...

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Veröffentlicht in:	Experimental cell research 1972-01, Vol.75 (1), p.39-46
Hauptverfasser:	Wulff, U.C., Desai, L.S., Foley, G.E.
Format:	Artikel
Sprache:	eng
Schlagworte:	Cells, Cultured Chromatography, Gel Electrophoresis, Polyacrylamide Gel Female Humans Infectious Mononucleosis - blood Leukemia, Lymphoid - blood Lymphocytes - analysis Male Methods Molecular Weight Nucleic Acid Denaturation Ribonucleotides - analysis RNA, Neoplasm - analysis RNA, Neoplasm - isolation & purification Spectrophotometry, Ultraviolet Temperature
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