A Capillary-tube Agglutination Test for the Detection and Titration of Theileria parva and Theileria mutans Antibodies in Bovine Serum
A capillary-tube agglutination test is described for the detection and titration of circulating antibodies stimulated in cattle as a result of Theileria parva and Theileria mutans infections. Particulate antigen homogenates of each organism were prepared by the ultrasonic disruption of parasitised e...
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| Veröffentlicht in: | Research in veterinary science 1972-09, Vol.13 (5), p.405-410 |
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| container_title | Research in veterinary science |
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| creator | Ross, J.P.J. Löhr, K-F. |
| description | A capillary-tube agglutination test is described for the detection and titration of circulating antibodies stimulated in cattle as a result of Theileria parva and Theileria mutans infections. Particulate antigen homogenates of each organism were prepared by the ultrasonic disruption of parasitised erythrocytes. Separation of soluble elements was effected by ultra-centrifugation. The test was validated by examining the antigens in the presence of a number of normal and immune sera, and sera from cattle suffering from a variety of local infections. Antibody responses in experimental infections of the two species were well distinguished. Cross-reactions were noted only in tests with the undiluted sera. Because of the stability of its antigens, its speed and operating simplicity, it is suggested as the routine test of choice for laboratory and field application. |
| doi_str_mv | 10.1016/S0034-5288(18)33983-3 |
| format | Article |
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Particulate antigen homogenates of each organism were prepared by the ultrasonic disruption of parasitised erythrocytes. Separation of soluble elements was effected by ultra-centrifugation. The test was validated by examining the antigens in the presence of a number of normal and immune sera, and sera from cattle suffering from a variety of local infections. Antibody responses in experimental infections of the two species were well distinguished. Cross-reactions were noted only in tests with the undiluted sera. Because of the stability of its antigens, its speed and operating simplicity, it is suggested as the routine test of choice for laboratory and field application.</description><identifier>ISSN: 0034-5288</identifier><identifier>EISSN: 1532-2661</identifier><identifier>DOI: 10.1016/S0034-5288(18)33983-3</identifier><identifier>PMID: 4564332</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Agglutination ; Agglutination Tests ; Animals ; Antibodies ; Antibodies - analysis ; Antibody Formation ; Antigen-Antibody Reactions ; Antigens ; Bovidae ; Capillary tubes ; Cattle ; Centrifugation ; Complement Fixation Tests ; Erythrocytes ; Fluorescent Antibody Technique ; Immune serum ; Infections ; Methods ; Theileria mutans ; Theileria parva ; Theileriasis - diagnosis ; Theileriasis - immunology ; Time Factors ; Titration ; Veterinary medicine</subject><ispartof>Research in veterinary science, 1972-09, Vol.13 (5), p.405-410</ispartof><rights>1972</rights><rights>Copyright Elsevier Limited Sep 1972</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c388t-962faf643c501f489f77775aa7c9f8ede79531eedd33ac473697e87c21e95eee3</citedby><cites>FETCH-LOGICAL-c388t-962faf643c501f489f77775aa7c9f8ede79531eedd33ac473697e87c21e95eee3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0034-5288(18)33983-3$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,45974</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/4564332$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ross, J.P.J.</creatorcontrib><creatorcontrib>Löhr, K-F.</creatorcontrib><title>A Capillary-tube Agglutination Test for the Detection and Titration of Theileria parva and Theileria mutans Antibodies in Bovine Serum</title><title>Research in veterinary science</title><addtitle>Res Vet Sci</addtitle><description>A capillary-tube agglutination test is described for the detection and titration of circulating antibodies stimulated in cattle as a result of Theileria parva and Theileria mutans infections. Particulate antigen homogenates of each organism were prepared by the ultrasonic disruption of parasitised erythrocytes. Separation of soluble elements was effected by ultra-centrifugation. The test was validated by examining the antigens in the presence of a number of normal and immune sera, and sera from cattle suffering from a variety of local infections. Antibody responses in experimental infections of the two species were well distinguished. Cross-reactions were noted only in tests with the undiluted sera. Because of the stability of its antigens, its speed and operating simplicity, it is suggested as the routine test of choice for laboratory and field application.</description><subject>Agglutination</subject><subject>Agglutination Tests</subject><subject>Animals</subject><subject>Antibodies</subject><subject>Antibodies - analysis</subject><subject>Antibody Formation</subject><subject>Antigen-Antibody Reactions</subject><subject>Antigens</subject><subject>Bovidae</subject><subject>Capillary tubes</subject><subject>Cattle</subject><subject>Centrifugation</subject><subject>Complement Fixation Tests</subject><subject>Erythrocytes</subject><subject>Fluorescent Antibody Technique</subject><subject>Immune serum</subject><subject>Infections</subject><subject>Methods</subject><subject>Theileria mutans</subject><subject>Theileria parva</subject><subject>Theileriasis - diagnosis</subject><subject>Theileriasis - immunology</subject><subject>Time Factors</subject><subject>Titration</subject><subject>Veterinary medicine</subject><issn>0034-5288</issn><issn>1532-2661</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1972</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkcFuGyEQQFHVKnXcfkIkpEpVe9gGloVlT5Xjpk2kSD3EPSPMDgnRLrjAWuoP9LtDvJYPvZQDSDOPYYaH0AUlXyih4vKeENZUvJbyE5WfGeskq9grtKCc1VUtBH2NFifkLTpP6YkQ0lDanqGzhouGsXqB_q7wWu_cMOj4p8rTFvDq4WGYsvM6u-DxBlLGNkScHwF_gwzmENa-xxuX4wwFizeP4AaITuOdjns9A6fYOGXtE1757Lahd5Cw8_gq7J0HfA9xGt-hN1YPCd4fzyX69f16s76p7n7-uF2v7irDpMxVJ2qrbWndcEJtIzvblsW1bk1nJfTQdpxRgL5nTJumZaJrQbamptBxAGBL9HGuu4vh91RmU6NLBsr4HsKUlKSCM0FFAT_8Az6FKfrSm6op6zhveSGXiM-UiSGlCFbtohvLVypK1IsldbCkXhQoKtXBUtmX6OJYfdqO0J9uHbWU_Nc5D-Uv9g6iSsaBN9C7WAyoPrj_vPAMoySioQ</recordid><startdate>197209</startdate><enddate>197209</enddate><creator>Ross, J.P.J.</creator><creator>Löhr, K-F.</creator><general>Elsevier Ltd</general><general>Elsevier Limited</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7QP</scope><scope>7QR</scope><scope>7T5</scope><scope>7T7</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>197209</creationdate><title>A Capillary-tube Agglutination Test for the Detection and Titration of Theileria parva and Theileria mutans Antibodies in Bovine Serum</title><author>Ross, J.P.J. ; Löhr, K-F.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c388t-962faf643c501f489f77775aa7c9f8ede79531eedd33ac473697e87c21e95eee3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1972</creationdate><topic>Agglutination</topic><topic>Agglutination Tests</topic><topic>Animals</topic><topic>Antibodies</topic><topic>Antibodies - analysis</topic><topic>Antibody Formation</topic><topic>Antigen-Antibody Reactions</topic><topic>Antigens</topic><topic>Bovidae</topic><topic>Capillary tubes</topic><topic>Cattle</topic><topic>Centrifugation</topic><topic>Complement Fixation Tests</topic><topic>Erythrocytes</topic><topic>Fluorescent Antibody Technique</topic><topic>Immune serum</topic><topic>Infections</topic><topic>Methods</topic><topic>Theileria mutans</topic><topic>Theileria parva</topic><topic>Theileriasis - diagnosis</topic><topic>Theileriasis - immunology</topic><topic>Time Factors</topic><topic>Titration</topic><topic>Veterinary medicine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ross, J.P.J.</creatorcontrib><creatorcontrib>Löhr, K-F.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Animal Behavior Abstracts</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Research in veterinary science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ross, J.P.J.</au><au>Löhr, K-F.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A Capillary-tube Agglutination Test for the Detection and Titration of Theileria parva and Theileria mutans Antibodies in Bovine Serum</atitle><jtitle>Research in veterinary science</jtitle><addtitle>Res Vet Sci</addtitle><date>1972-09</date><risdate>1972</risdate><volume>13</volume><issue>5</issue><spage>405</spage><epage>410</epage><pages>405-410</pages><issn>0034-5288</issn><eissn>1532-2661</eissn><abstract>A capillary-tube agglutination test is described for the detection and titration of circulating antibodies stimulated in cattle as a result of Theileria parva and Theileria mutans infections. Particulate antigen homogenates of each organism were prepared by the ultrasonic disruption of parasitised erythrocytes. Separation of soluble elements was effected by ultra-centrifugation. The test was validated by examining the antigens in the presence of a number of normal and immune sera, and sera from cattle suffering from a variety of local infections. Antibody responses in experimental infections of the two species were well distinguished. Cross-reactions were noted only in tests with the undiluted sera. Because of the stability of its antigens, its speed and operating simplicity, it is suggested as the routine test of choice for laboratory and field application.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>4564332</pmid><doi>10.1016/S0034-5288(18)33983-3</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0034-5288 |
| ispartof | Research in veterinary science, 1972-09, Vol.13 (5), p.405-410 |
| issn | 0034-5288 1532-2661 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Agglutination Agglutination Tests Animals Antibodies Antibodies - analysis Antibody Formation Antigen-Antibody Reactions Antigens Bovidae Capillary tubes Cattle Centrifugation Complement Fixation Tests Erythrocytes Fluorescent Antibody Technique Immune serum Infections Methods Theileria mutans Theileria parva Theileriasis - diagnosis Theileriasis - immunology Time Factors Titration Veterinary medicine |
| title | A Capillary-tube Agglutination Test for the Detection and Titration of Theileria parva and Theileria mutans Antibodies in Bovine Serum |
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