Preparation of rat liver cells: I. Effect of Ca 2+ on enzymatic dispersion of isolated, perfused liver

Suspensions of rat liver cells were prepared by perfusion of the isolated liver with collagenase and hyaluronidase. By means of a novel dispersion assay, which measured swelling of the liver in a closed perfusion system, the time course of enzymatic dispersion could be followed. Ca 2+ stimulated the...

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Veröffentlicht in:	Experimental cell research 1972-10, Vol.74 (2), p.450-454
1. Verfasser:	Seglen, P.O.
Format:	Artikel
Sprache:	eng
Schlagworte:	Acetates - pharmacology Animals Calcium - pharmacology Glycols - pharmacology Hyaluronoglucosaminidase - pharmacology In Vitro Techniques Kinetics Liver - cytology Liver - drug effects Male Methods Microbial Collagenase - pharmacology Perfusion - instrumentation Rats
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container_title	Experimental cell research
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creator	Seglen, P.O.
description	Suspensions of rat liver cells were prepared by perfusion of the isolated liver with collagenase and hyaluronidase. By means of a novel dispersion assay, which measured swelling of the liver in a closed perfusion system, the time course of enzymatic dispersion could be followed. Ca 2+ stimulated the enzymatic dispersion strongly, but a preliminary removal of Ca 2+ with the chelator EGTA rendered the liver tissue more susceptible to the action of enzymes. The best result was thus obtained when the liver was first perfused 5 min with EGTA, then 5 min with enzymes and Ca 2+. This sequential treatment converted the whole liver to a cellular suspension, in which about 95% of the cells were intact.
doi_str_mv	10.1016/0014-4827(72)90400-4
format	Article
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This sequential treatment converted the whole liver to a cellular suspension, in which about 95% of the cells were intact.</description><subject>Acetates - pharmacology</subject><subject>Animals</subject><subject>Calcium - pharmacology</subject><subject>Glycols - pharmacology</subject><subject>Hyaluronoglucosaminidase - pharmacology</subject><subject>In Vitro Techniques</subject><subject>Kinetics</subject><subject>Liver - cytology</subject><subject>Liver - drug effects</subject><subject>Male</subject><subject>Methods</subject><subject>Microbial Collagenase - pharmacology</subject><subject>Perfusion - instrumentation</subject><subject>Rats</subject><issn>0014-4827</issn><issn>1090-2422</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1972</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9UdtKAzEQDaLUWv0DhTyJolsnl735IEjxUijogz6HbHYCke3umrSF-vVmbfFphjlnDnPOEHLOYMqAZXcATCay4PlVzq9LkACJPCBjBiUkXHJ-SMb_lGNyEsIXABQFy0ZkJIUUwGFM7LvHXnu9cl1LO0tjRxu3QU8NNk24p_MpfbIWzWpAZ5ryGxqZ2P5sl3HJ0NqFHn3Yr7vQNXqF9S2NQ7sOWO_UTsmR1U3As32dkM_np4_Za7J4e5nPHhcJMiggsbKAgtc5gpFClJBmyNNSFFBVphSa2VTbTBhuhKilTk2FFYJluuQFGsFLMSGXO93ed99rDCu1dGFwolvs1kFF94xBBpF4sSeuqyXWqvduqf1W7YOJ-MMOx3jtxqFXwThsDdbOxzBU3TnFQA2PUEPKakhZ5Vz9PSLK_AKMdHhZ</recordid><startdate>197210</startdate><enddate>197210</enddate><creator>Seglen, P.O.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>197210</creationdate><title>Preparation of rat liver cells: I. Effect of Ca 2+ on enzymatic dispersion of isolated, perfused liver</title><author>Seglen, P.O.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-e1080-f48082d7e0c4339056e259380bbc93a1f5af63c2c33d4a5cbebe0f1a928ec3293</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1972</creationdate><topic>Acetates - pharmacology</topic><topic>Animals</topic><topic>Calcium - pharmacology</topic><topic>Glycols - pharmacology</topic><topic>Hyaluronoglucosaminidase - pharmacology</topic><topic>In Vitro Techniques</topic><topic>Kinetics</topic><topic>Liver - cytology</topic><topic>Liver - drug effects</topic><topic>Male</topic><topic>Methods</topic><topic>Microbial Collagenase - pharmacology</topic><topic>Perfusion - instrumentation</topic><topic>Rats</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Seglen, P.O.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Experimental cell research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Seglen, P.O.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Preparation of rat liver cells: I. 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This sequential treatment converted the whole liver to a cellular suspension, in which about 95% of the cells were intact.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>4343020</pmid><doi>10.1016/0014-4827(72)90400-4</doi><tpages>5</tpages></addata></record>
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subjects	Acetates - pharmacology Animals Calcium - pharmacology Glycols - pharmacology Hyaluronoglucosaminidase - pharmacology In Vitro Techniques Kinetics Liver - cytology Liver - drug effects Male Methods Microbial Collagenase - pharmacology Perfusion - instrumentation Rats
title	Preparation of rat liver cells: I. Effect of Ca 2+ on enzymatic dispersion of isolated, perfused liver
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