Green tea polyphenols attenuating ultraviolet B-induced damage to human retinal pigment epithelial cells in vitro
To examine the protective effect of green tea polyphenols against ultraviolet B (UVB)-induced damage to retinal pigment epithelial (RPE) cells. Green tea polyphenols (GTP) was used to treat RPE cells before or after exposure to UVB. Viability of RPE cells was tested by 3,(4,5-dimethylthiazol-2-yl)-2...
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| Veröffentlicht in: | Investigative ophthalmology & visual science 2010-12, Vol.51 (12), p.6665-6670 |
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| container_title | Investigative ophthalmology & visual science |
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| creator | Xu, Jing-Yi Wu, Liang-Yu Zheng, Xin-Qiang Lu, Jian-Liang Wu, Ming-Yan Liang, Yue-Rong |
| description | To examine the protective effect of green tea polyphenols against ultraviolet B (UVB)-induced damage to retinal pigment epithelial (RPE) cells.
Green tea polyphenols (GTP) was used to treat RPE cells before or after exposure to UVB. Viability of RPE cells was tested by 3,(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) assay. Survivin gene expression was examined by real-time PCR analysis. Ultrastructure of RPE cells was examined by transmission electron microscopy.
GTP effectively suppressed the decrease in viability of the UVB stressed RPE cells and the UVB suppression of survivin gene expression level. GTP alleviated mitochondria dysfunction and DNA fragmentation induced by UVB.
GTP protected RPE cells from UVB damage through its increase in the survivin gene expression and its attenuation of mitochondria dysfunction and DNA fragmentation. GTP is a potential candidate for further development as a chemoprotective factor for the primary prevention of age-related eye diseases such as age-related macular degeneration. |
| doi_str_mv | 10.1167/iovs.10-5698 |
| format | Article |
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Green tea polyphenols (GTP) was used to treat RPE cells before or after exposure to UVB. Viability of RPE cells was tested by 3,(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) assay. Survivin gene expression was examined by real-time PCR analysis. Ultrastructure of RPE cells was examined by transmission electron microscopy.
GTP effectively suppressed the decrease in viability of the UVB stressed RPE cells and the UVB suppression of survivin gene expression level. GTP alleviated mitochondria dysfunction and DNA fragmentation induced by UVB.
GTP protected RPE cells from UVB damage through its increase in the survivin gene expression and its attenuation of mitochondria dysfunction and DNA fragmentation. GTP is a potential candidate for further development as a chemoprotective factor for the primary prevention of age-related eye diseases such as age-related macular degeneration.</description><identifier>ISSN: 1552-5783</identifier><identifier>EISSN: 1552-5783</identifier><identifier>DOI: 10.1167/iovs.10-5698</identifier><identifier>PMID: 20702817</identifier><language>eng</language><publisher>United States</publisher><subject>Camellia sinensis ; Cell Survival - radiation effects ; Cells, Cultured ; DNA Fragmentation - drug effects ; Flavonoids - pharmacology ; Formazans ; Gene Expression ; Humans ; Inhibitor of Apoptosis Proteins ; Microtubule-Associated Proteins - genetics ; Mitochondrial Diseases - prevention & control ; Phenols - pharmacology ; Polyphenols ; Radiation-Protective Agents - pharmacology ; Retinal Pigment Epithelium - drug effects ; Retinal Pigment Epithelium - radiation effects ; Retinal Pigment Epithelium - ultrastructure ; Reverse Transcriptase Polymerase Chain Reaction ; Tea ; Tetrazolium Salts ; Ultraviolet Rays</subject><ispartof>Investigative ophthalmology & visual science, 2010-12, Vol.51 (12), p.6665-6670</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c290t-b8906142ee68dbd98a165a6c6c61f2e08a74f83d3ce34b4c4acf00f0de074dbd3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27922,27923</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20702817$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Xu, Jing-Yi</creatorcontrib><creatorcontrib>Wu, Liang-Yu</creatorcontrib><creatorcontrib>Zheng, Xin-Qiang</creatorcontrib><creatorcontrib>Lu, Jian-Liang</creatorcontrib><creatorcontrib>Wu, Ming-Yan</creatorcontrib><creatorcontrib>Liang, Yue-Rong</creatorcontrib><title>Green tea polyphenols attenuating ultraviolet B-induced damage to human retinal pigment epithelial cells in vitro</title><title>Investigative ophthalmology & visual science</title><addtitle>Invest Ophthalmol Vis Sci</addtitle><description>To examine the protective effect of green tea polyphenols against ultraviolet B (UVB)-induced damage to retinal pigment epithelial (RPE) cells.
Green tea polyphenols (GTP) was used to treat RPE cells before or after exposure to UVB. Viability of RPE cells was tested by 3,(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) assay. Survivin gene expression was examined by real-time PCR analysis. Ultrastructure of RPE cells was examined by transmission electron microscopy.
GTP effectively suppressed the decrease in viability of the UVB stressed RPE cells and the UVB suppression of survivin gene expression level. GTP alleviated mitochondria dysfunction and DNA fragmentation induced by UVB.
GTP protected RPE cells from UVB damage through its increase in the survivin gene expression and its attenuation of mitochondria dysfunction and DNA fragmentation. GTP is a potential candidate for further development as a chemoprotective factor for the primary prevention of age-related eye diseases such as age-related macular degeneration.</description><subject>Camellia sinensis</subject><subject>Cell Survival - radiation effects</subject><subject>Cells, Cultured</subject><subject>DNA Fragmentation - drug effects</subject><subject>Flavonoids - pharmacology</subject><subject>Formazans</subject><subject>Gene Expression</subject><subject>Humans</subject><subject>Inhibitor of Apoptosis Proteins</subject><subject>Microtubule-Associated Proteins - genetics</subject><subject>Mitochondrial Diseases - prevention & control</subject><subject>Phenols - pharmacology</subject><subject>Polyphenols</subject><subject>Radiation-Protective Agents - pharmacology</subject><subject>Retinal Pigment Epithelium - drug effects</subject><subject>Retinal Pigment Epithelium - radiation effects</subject><subject>Retinal Pigment Epithelium - ultrastructure</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>Tea</subject><subject>Tetrazolium Salts</subject><subject>Ultraviolet Rays</subject><issn>1552-5783</issn><issn>1552-5783</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpNkMFLwzAUh4Mobk5vniU3L3YmadqmRx06hYEXPZcsfd0iadIl6WD_vS2bIjm8vMf3fjw-hG4pmVOaF4_a7cOckiTLS3GGpjTLWJIVIj3_95-gqxC-CWGUMnKJJowUhAlaTNFu6QEsjiBx58yh24J1JmAZI9heRm03uDfRy712BiJ-TrStewU1rmUrN4Cjw9u-lRZ7GGBpcKc3LdiIodNxC0YPIwVmiNQW73X07hpdNNIEuDnVGfp6fflcvCWrj-X74mmVKFaSmKxFSXLKGUAu6nVdCknzTOZqeLRhQIQseCPSOlWQ8jVXXKqGkIbUQAo-LKQzdH_M7bzb9RBi1eowniItuD5UgmZlzlNeDuTDkVTeheChqTqvW-kPFSXV6LgaHY_N6HjA707B_bqF-g_-lZr-ANOxevo</recordid><startdate>201012</startdate><enddate>201012</enddate><creator>Xu, Jing-Yi</creator><creator>Wu, Liang-Yu</creator><creator>Zheng, Xin-Qiang</creator><creator>Lu, Jian-Liang</creator><creator>Wu, Ming-Yan</creator><creator>Liang, Yue-Rong</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201012</creationdate><title>Green tea polyphenols attenuating ultraviolet B-induced damage to human retinal pigment epithelial cells in vitro</title><author>Xu, Jing-Yi ; Wu, Liang-Yu ; Zheng, Xin-Qiang ; Lu, Jian-Liang ; Wu, Ming-Yan ; Liang, Yue-Rong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c290t-b8906142ee68dbd98a165a6c6c61f2e08a74f83d3ce34b4c4acf00f0de074dbd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Camellia sinensis</topic><topic>Cell Survival - radiation effects</topic><topic>Cells, Cultured</topic><topic>DNA Fragmentation - drug effects</topic><topic>Flavonoids - pharmacology</topic><topic>Formazans</topic><topic>Gene Expression</topic><topic>Humans</topic><topic>Inhibitor of Apoptosis Proteins</topic><topic>Microtubule-Associated Proteins - genetics</topic><topic>Mitochondrial Diseases - prevention & control</topic><topic>Phenols - pharmacology</topic><topic>Polyphenols</topic><topic>Radiation-Protective Agents - pharmacology</topic><topic>Retinal Pigment Epithelium - drug effects</topic><topic>Retinal Pigment Epithelium - radiation effects</topic><topic>Retinal Pigment Epithelium - ultrastructure</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>Tea</topic><topic>Tetrazolium Salts</topic><topic>Ultraviolet Rays</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Xu, Jing-Yi</creatorcontrib><creatorcontrib>Wu, Liang-Yu</creatorcontrib><creatorcontrib>Zheng, Xin-Qiang</creatorcontrib><creatorcontrib>Lu, Jian-Liang</creatorcontrib><creatorcontrib>Wu, Ming-Yan</creatorcontrib><creatorcontrib>Liang, Yue-Rong</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Investigative ophthalmology & visual science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Xu, Jing-Yi</au><au>Wu, Liang-Yu</au><au>Zheng, Xin-Qiang</au><au>Lu, Jian-Liang</au><au>Wu, Ming-Yan</au><au>Liang, Yue-Rong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Green tea polyphenols attenuating ultraviolet B-induced damage to human retinal pigment epithelial cells in vitro</atitle><jtitle>Investigative ophthalmology & visual science</jtitle><addtitle>Invest Ophthalmol Vis Sci</addtitle><date>2010-12</date><risdate>2010</risdate><volume>51</volume><issue>12</issue><spage>6665</spage><epage>6670</epage><pages>6665-6670</pages><issn>1552-5783</issn><eissn>1552-5783</eissn><abstract>To examine the protective effect of green tea polyphenols against ultraviolet B (UVB)-induced damage to retinal pigment epithelial (RPE) cells.
Green tea polyphenols (GTP) was used to treat RPE cells before or after exposure to UVB. Viability of RPE cells was tested by 3,(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) assay. Survivin gene expression was examined by real-time PCR analysis. Ultrastructure of RPE cells was examined by transmission electron microscopy.
GTP effectively suppressed the decrease in viability of the UVB stressed RPE cells and the UVB suppression of survivin gene expression level. GTP alleviated mitochondria dysfunction and DNA fragmentation induced by UVB.
GTP protected RPE cells from UVB damage through its increase in the survivin gene expression and its attenuation of mitochondria dysfunction and DNA fragmentation. GTP is a potential candidate for further development as a chemoprotective factor for the primary prevention of age-related eye diseases such as age-related macular degeneration.</abstract><cop>United States</cop><pmid>20702817</pmid><doi>10.1167/iovs.10-5698</doi><tpages>6</tpages></addata></record> |
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| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | Camellia sinensis Cell Survival - radiation effects Cells, Cultured DNA Fragmentation - drug effects Flavonoids - pharmacology Formazans Gene Expression Humans Inhibitor of Apoptosis Proteins Microtubule-Associated Proteins - genetics Mitochondrial Diseases - prevention & control Phenols - pharmacology Polyphenols Radiation-Protective Agents - pharmacology Retinal Pigment Epithelium - drug effects Retinal Pigment Epithelium - radiation effects Retinal Pigment Epithelium - ultrastructure Reverse Transcriptase Polymerase Chain Reaction Tea Tetrazolium Salts Ultraviolet Rays |
| title | Green tea polyphenols attenuating ultraviolet B-induced damage to human retinal pigment epithelial cells in vitro |
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