role of thrombospondin-1-mediated TGF-β1 on collagen type III synthesis induced by high glucose
Transforming growth factor-β1 (TGF-β1) has been thought to play a major role during cardiac fibrosis in the development of diabetic cardiomyopathy, and cardiac fibrosis mainly as a result of an increase of collagen type III occurs in the human hearts with diabetes. Thrombospondin-1 (TSP-1) has been...
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| Veröffentlicht in: | Molecular and cellular biochemistry 2011, Vol.346 (1-2), p.49-56 |
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| creator | Tang, Mengxiong Zhou, Fenghua Zhang, Wei Guo, Zhongxiu Shang, Yuanyuan Lu, Huixia Lu, Ruijuan Zhang, Yun Chen, Yuguo Zhong, Ming |
| description | Transforming growth factor-β1 (TGF-β1) has been thought to play a major role during cardiac fibrosis in the development of diabetic cardiomyopathy, and cardiac fibrosis mainly as a result of an increase of collagen type III occurs in the human hearts with diabetes. Thrombospondin-1 (TSP-1) has been reported to activate the latent complex of TGF-β1. We examined the effects of TSP-1 on the expression of TGF-β1 and collagen type III by rat cardiac fibroblasts in high ambient glucose. We demonstrated that high glucose induces the mRNA and protein expression of collagen type III, TGF-β1, and TSP-1. Furthermore, the mRNA and protein expression of collagen type III induced by high glucose was downregulated after treatment with TGF-β1 antibody, or TSP-1 siRNA. The expression of TGF-β1 increased by high glucose was also reversed after treatment with TSP-1 siRNA. Our findings suggest that the TSP-1 participates in the upregulation of TGF-β1, collagen type III by high glucose and may provide new therapeutic strategies for diabetic cardiomyopathy. |
| doi_str_mv | 10.1007/s11010-010-0590-7 |
| format | Article |
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Thrombospondin-1 (TSP-1) has been reported to activate the latent complex of TGF-β1. We examined the effects of TSP-1 on the expression of TGF-β1 and collagen type III by rat cardiac fibroblasts in high ambient glucose. We demonstrated that high glucose induces the mRNA and protein expression of collagen type III, TGF-β1, and TSP-1. Furthermore, the mRNA and protein expression of collagen type III induced by high glucose was downregulated after treatment with TGF-β1 antibody, or TSP-1 siRNA. The expression of TGF-β1 increased by high glucose was also reversed after treatment with TSP-1 siRNA. Our findings suggest that the TSP-1 participates in the upregulation of TGF-β1, collagen type III by high glucose and may provide new therapeutic strategies for diabetic cardiomyopathy.</description><identifier>ISSN: 0300-8177</identifier><identifier>EISSN: 1573-4919</identifier><identifier>DOI: 10.1007/s11010-010-0590-7</identifier><identifier>PMID: 20878350</identifier><language>eng</language><publisher>Boston: Boston : Springer US</publisher><subject>Animals ; Base Sequence ; Biochemistry ; Biomedical and Life Sciences ; Blotting, Western ; Bone morphogenetic proteins ; Cardiac fibroblasts ; Cardiology ; Collagen ; Collagen type III ; Collagen Type III - biosynthesis ; Dextrose ; DNA Primers ; Enzyme-Linked Immunosorbent Assay ; Glucose ; Glucose - administration & dosage ; Glucose metabolism ; Life Sciences ; Male ; Medical Biochemistry ; Oncology ; Rats ; Rats, Wistar ; Reverse Transcriptase Polymerase Chain Reaction ; RNA ; RNA, Small Interfering ; Thrombospondin 1 - genetics ; Thrombospondin 1 - physiology ; Thrombospondin-1 ; Transforming Growth Factor beta1 - physiology ; Transforming growth factor-β1 ; Transforming growth factors</subject><ispartof>Molecular and cellular biochemistry, 2011, Vol.346 (1-2), p.49-56</ispartof><rights>Springer Science+Business Media, LLC. 2010</rights><rights>COPYRIGHT 2011 Springer</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3497-2c226ef4aa833c92687a55bcc3f5dce643641c6d78d7f2eebaf1b78710832ab3</citedby><cites>FETCH-LOGICAL-c3497-2c226ef4aa833c92687a55bcc3f5dce643641c6d78d7f2eebaf1b78710832ab3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11010-010-0590-7$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11010-010-0590-7$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,4010,27900,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20878350$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tang, Mengxiong</creatorcontrib><creatorcontrib>Zhou, Fenghua</creatorcontrib><creatorcontrib>Zhang, Wei</creatorcontrib><creatorcontrib>Guo, Zhongxiu</creatorcontrib><creatorcontrib>Shang, Yuanyuan</creatorcontrib><creatorcontrib>Lu, Huixia</creatorcontrib><creatorcontrib>Lu, Ruijuan</creatorcontrib><creatorcontrib>Zhang, Yun</creatorcontrib><creatorcontrib>Chen, Yuguo</creatorcontrib><creatorcontrib>Zhong, Ming</creatorcontrib><title>role of thrombospondin-1-mediated TGF-β1 on collagen type III synthesis induced by high glucose</title><title>Molecular and cellular biochemistry</title><addtitle>Mol Cell Biochem</addtitle><addtitle>Mol Cell Biochem</addtitle><description>Transforming growth factor-β1 (TGF-β1) has been thought to play a major role during cardiac fibrosis in the development of diabetic cardiomyopathy, and cardiac fibrosis mainly as a result of an increase of collagen type III occurs in the human hearts with diabetes. Thrombospondin-1 (TSP-1) has been reported to activate the latent complex of TGF-β1. We examined the effects of TSP-1 on the expression of TGF-β1 and collagen type III by rat cardiac fibroblasts in high ambient glucose. We demonstrated that high glucose induces the mRNA and protein expression of collagen type III, TGF-β1, and TSP-1. Furthermore, the mRNA and protein expression of collagen type III induced by high glucose was downregulated after treatment with TGF-β1 antibody, or TSP-1 siRNA. The expression of TGF-β1 increased by high glucose was also reversed after treatment with TSP-1 siRNA. Our findings suggest that the TSP-1 participates in the upregulation of TGF-β1, collagen type III by high glucose and may provide new therapeutic strategies for diabetic cardiomyopathy.</description><subject>Animals</subject><subject>Base Sequence</subject><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Blotting, Western</subject><subject>Bone morphogenetic proteins</subject><subject>Cardiac fibroblasts</subject><subject>Cardiology</subject><subject>Collagen</subject><subject>Collagen type III</subject><subject>Collagen Type III - biosynthesis</subject><subject>Dextrose</subject><subject>DNA Primers</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Glucose</subject><subject>Glucose - administration & dosage</subject><subject>Glucose metabolism</subject><subject>Life Sciences</subject><subject>Male</subject><subject>Medical Biochemistry</subject><subject>Oncology</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA</subject><subject>RNA, Small Interfering</subject><subject>Thrombospondin 1 - genetics</subject><subject>Thrombospondin 1 - physiology</subject><subject>Thrombospondin-1</subject><subject>Transforming Growth Factor beta1 - physiology</subject><subject>Transforming growth factor-β1</subject><subject>Transforming growth factors</subject><issn>0300-8177</issn><issn>1573-4919</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kcFu1DAQhi0EokvhAbiAJQ49uXjiJLaPVUXLSpU4sJyN49hZV4m92MlhX4sH4ZnqbQoSEkLWyJL9faPR_Ai9BXoJlPKPGYACJY_VSEr4M7SBhjNSS5DP0YYySokAzs_Qq5zvaQEpwEt0VlHBBWvoBn1PcbQ4OjzvU5y6mA8x9D4QIJPtvZ5tj3e3N-TXT8AxYBPHUQ824Pl4sHi73eJ8DPPeZp-xD_1iCt4d8d4PezyMi4nZvkYvnB6zffN0n6Pdzafd9Wdy9-V2e311RwyrJSeVqarWulprwZiRVSu4bprOGOaa3ti2Zm0Npu256LmrrO20g44LDlSwSnfsHF2sbQ8p_lhsntXks7Fl3GDjkpWARrZMsqaQH1Zy0KNVPrg4J21OtLpibUVlQ8WJuvwHVU5vJ29isM6X978EWAWTYs7JOnVIftLpqICqU1pqTUs9VklL8eK8exp66cq6_xi_4ylAtQK5fIXBJnUflxTKHv_b9f0qOR2VHpLP6tvXigKjIIG3tWQPYYWnNw</recordid><startdate>2011</startdate><enddate>2011</enddate><creator>Tang, Mengxiong</creator><creator>Zhou, Fenghua</creator><creator>Zhang, Wei</creator><creator>Guo, Zhongxiu</creator><creator>Shang, Yuanyuan</creator><creator>Lu, Huixia</creator><creator>Lu, Ruijuan</creator><creator>Zhang, Yun</creator><creator>Chen, Yuguo</creator><creator>Zhong, Ming</creator><general>Boston : Springer US</general><general>Springer US</general><general>Springer</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>2011</creationdate><title>role of thrombospondin-1-mediated TGF-β1 on collagen type III synthesis induced by high glucose</title><author>Tang, Mengxiong ; Zhou, Fenghua ; Zhang, Wei ; Guo, Zhongxiu ; Shang, Yuanyuan ; Lu, Huixia ; Lu, Ruijuan ; Zhang, Yun ; Chen, Yuguo ; Zhong, Ming</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3497-2c226ef4aa833c92687a55bcc3f5dce643641c6d78d7f2eebaf1b78710832ab3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Animals</topic><topic>Base Sequence</topic><topic>Biochemistry</topic><topic>Biomedical and Life Sciences</topic><topic>Blotting, Western</topic><topic>Bone morphogenetic proteins</topic><topic>Cardiac fibroblasts</topic><topic>Cardiology</topic><topic>Collagen</topic><topic>Collagen type III</topic><topic>Collagen Type III - biosynthesis</topic><topic>Dextrose</topic><topic>DNA Primers</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Glucose</topic><topic>Glucose - administration & dosage</topic><topic>Glucose metabolism</topic><topic>Life Sciences</topic><topic>Male</topic><topic>Medical Biochemistry</topic><topic>Oncology</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA</topic><topic>RNA, Small Interfering</topic><topic>Thrombospondin 1 - genetics</topic><topic>Thrombospondin 1 - physiology</topic><topic>Thrombospondin-1</topic><topic>Transforming Growth Factor beta1 - physiology</topic><topic>Transforming growth factor-β1</topic><topic>Transforming growth factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tang, Mengxiong</creatorcontrib><creatorcontrib>Zhou, Fenghua</creatorcontrib><creatorcontrib>Zhang, Wei</creatorcontrib><creatorcontrib>Guo, Zhongxiu</creatorcontrib><creatorcontrib>Shang, Yuanyuan</creatorcontrib><creatorcontrib>Lu, Huixia</creatorcontrib><creatorcontrib>Lu, Ruijuan</creatorcontrib><creatorcontrib>Zhang, Yun</creatorcontrib><creatorcontrib>Chen, Yuguo</creatorcontrib><creatorcontrib>Zhong, Ming</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular and cellular biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tang, Mengxiong</au><au>Zhou, Fenghua</au><au>Zhang, Wei</au><au>Guo, Zhongxiu</au><au>Shang, Yuanyuan</au><au>Lu, Huixia</au><au>Lu, Ruijuan</au><au>Zhang, Yun</au><au>Chen, Yuguo</au><au>Zhong, Ming</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>role of thrombospondin-1-mediated TGF-β1 on collagen type III synthesis induced by high glucose</atitle><jtitle>Molecular and cellular biochemistry</jtitle><stitle>Mol Cell Biochem</stitle><addtitle>Mol Cell Biochem</addtitle><date>2011</date><risdate>2011</risdate><volume>346</volume><issue>1-2</issue><spage>49</spage><epage>56</epage><pages>49-56</pages><issn>0300-8177</issn><eissn>1573-4919</eissn><abstract>Transforming growth factor-β1 (TGF-β1) has been thought to play a major role during cardiac fibrosis in the development of diabetic cardiomyopathy, and cardiac fibrosis mainly as a result of an increase of collagen type III occurs in the human hearts with diabetes. 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| ispartof | Molecular and cellular biochemistry, 2011, Vol.346 (1-2), p.49-56 |
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| subjects | Animals Base Sequence Biochemistry Biomedical and Life Sciences Blotting, Western Bone morphogenetic proteins Cardiac fibroblasts Cardiology Collagen Collagen type III Collagen Type III - biosynthesis Dextrose DNA Primers Enzyme-Linked Immunosorbent Assay Glucose Glucose - administration & dosage Glucose metabolism Life Sciences Male Medical Biochemistry Oncology Rats Rats, Wistar Reverse Transcriptase Polymerase Chain Reaction RNA RNA, Small Interfering Thrombospondin 1 - genetics Thrombospondin 1 - physiology Thrombospondin-1 Transforming Growth Factor beta1 - physiology Transforming growth factor-β1 Transforming growth factors |
| title | role of thrombospondin-1-mediated TGF-β1 on collagen type III synthesis induced by high glucose |
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