Observations on the heat-stability of alkaline phosphatase isoenzymes in serum
Serum specimens which apparently contain the same isoenzyme of alkaline phosphatase nevertheless show wide variations in the stabilities of their phosphatase activities on heating at 56°. A number of factors which may influence the stability of bone and liver phosphatases in serum have been studied...
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| Veröffentlicht in: | Clinica chimica acta 1972-08, Vol.40 (1), p.35-41 |
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| container_title | Clinica chimica acta |
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| creator | Moss, D.W. Shakespeare, M.J. Thomas, Dilys M. |
| description | Serum specimens which apparently contain the same isoenzyme of alkaline phosphatase nevertheless show wide variations in the stabilities of their phosphatase activities on heating at 56°. A number of factors which may influence the stability of bone and liver phosphatases in serum have been studied and their contributions to the observed variability of serum phosphatase heat-stabilities have been assessed. Differences between specimens in protein or urea content do not appear to be significant in this respect, but differences in pH may cause variability in enzyme heat-stability. When differences between serum samples in characteristics such as pH have been taken into account, however, the range of variation which still remains suggests that a particular phosphatase isoenzyme entering the circulation in different individuals and in various diseases may not have constant stability properties. |
| doi_str_mv | 10.1016/0009-8981(72)90248-3 |
| format | Article |
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A number of factors which may influence the stability of bone and liver phosphatases in serum have been studied and their contributions to the observed variability of serum phosphatase heat-stabilities have been assessed. Differences between specimens in protein or urea content do not appear to be significant in this respect, but differences in pH may cause variability in enzyme heat-stability. When differences between serum samples in characteristics such as pH have been taken into account, however, the range of variation which still remains suggests that a particular phosphatase isoenzyme entering the circulation in different individuals and in various diseases may not have constant stability properties.</description><identifier>ISSN: 0009-8981</identifier><identifier>EISSN: 1873-3492</identifier><identifier>DOI: 10.1016/0009-8981(72)90248-3</identifier><identifier>PMID: 5056641</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Alkaline Phosphatase - blood ; Autoanalysis ; Bone and Bones - enzymology ; Drug Stability ; Electrophoresis, Starch Gel ; Hot Temperature ; Humans ; Hydrogen-Ion Concentration ; Isoenzymes - blood ; Liver - enzymology ; Osmolar Concentration ; Serum Albumin, Bovine - analysis ; Urea - blood</subject><ispartof>Clinica chimica acta, 1972-08, Vol.40 (1), p.35-41</ispartof><rights>1972</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c357t-9130ba19bc4943b04da91447ebc3d5b3cc8d1fafdf3b47beb736440b060f0c093</citedby><cites>FETCH-LOGICAL-c357t-9130ba19bc4943b04da91447ebc3d5b3cc8d1fafdf3b47beb736440b060f0c093</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0009-8981(72)90248-3$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,27923,27924,45994</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/5056641$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Moss, D.W.</creatorcontrib><creatorcontrib>Shakespeare, M.J.</creatorcontrib><creatorcontrib>Thomas, Dilys M.</creatorcontrib><title>Observations on the heat-stability of alkaline phosphatase isoenzymes in serum</title><title>Clinica chimica acta</title><addtitle>Clin Chim Acta</addtitle><description>Serum specimens which apparently contain the same isoenzyme of alkaline phosphatase nevertheless show wide variations in the stabilities of their phosphatase activities on heating at 56°. A number of factors which may influence the stability of bone and liver phosphatases in serum have been studied and their contributions to the observed variability of serum phosphatase heat-stabilities have been assessed. Differences between specimens in protein or urea content do not appear to be significant in this respect, but differences in pH may cause variability in enzyme heat-stability. When differences between serum samples in characteristics such as pH have been taken into account, however, the range of variation which still remains suggests that a particular phosphatase isoenzyme entering the circulation in different individuals and in various diseases may not have constant stability properties.</description><subject>Alkaline Phosphatase - blood</subject><subject>Autoanalysis</subject><subject>Bone and Bones - enzymology</subject><subject>Drug Stability</subject><subject>Electrophoresis, Starch Gel</subject><subject>Hot Temperature</subject><subject>Humans</subject><subject>Hydrogen-Ion Concentration</subject><subject>Isoenzymes - blood</subject><subject>Liver - enzymology</subject><subject>Osmolar Concentration</subject><subject>Serum Albumin, Bovine - analysis</subject><subject>Urea - blood</subject><issn>0009-8981</issn><issn>1873-3492</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1972</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kM9LwzAUx4Moc07_A4WcRA_VpEnb5CLI8BcMd9FzSNJXGm2b2aSD-dfbueHR0-Px_fF4H4TOKbmhhOa3hBCZCCnoVZFeS5JykbADNKWiYAnjMj1E0z_LMToJ4WNcOcnpBE0ykuU5p1P0ujQB-rWOzncB-w7HGnANOiYhauMaFzfYV1g3n7pxHeBV7cOq1lEHwC546L43LQTsOjzWDO0pOqp0E-BsP2fo_fHhbf6cLJZPL_P7RWJZVsREUkaMptJYLjkzhJdaUs4LMJaVmWHWipJWuiorZnhhwBQs55wYkpOKWCLZDF3uele9_xogRNW6YKFpdAd-CErQTDBB-WjkO6PtfQg9VGrVu1b3G0WJ2mJUW0Zqy0gVqfrFqNgYu9j3D6aF8i-05zbqdzsdxifXDnoVrIPOQul6sFGV3v1_4AdGRoKG</recordid><startdate>197208</startdate><enddate>197208</enddate><creator>Moss, D.W.</creator><creator>Shakespeare, M.J.</creator><creator>Thomas, Dilys M.</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>197208</creationdate><title>Observations on the heat-stability of alkaline phosphatase isoenzymes in serum</title><author>Moss, D.W. ; Shakespeare, M.J. ; Thomas, Dilys M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c357t-9130ba19bc4943b04da91447ebc3d5b3cc8d1fafdf3b47beb736440b060f0c093</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1972</creationdate><topic>Alkaline Phosphatase - blood</topic><topic>Autoanalysis</topic><topic>Bone and Bones - enzymology</topic><topic>Drug Stability</topic><topic>Electrophoresis, Starch Gel</topic><topic>Hot Temperature</topic><topic>Humans</topic><topic>Hydrogen-Ion Concentration</topic><topic>Isoenzymes - blood</topic><topic>Liver - enzymology</topic><topic>Osmolar Concentration</topic><topic>Serum Albumin, Bovine - analysis</topic><topic>Urea - blood</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Moss, D.W.</creatorcontrib><creatorcontrib>Shakespeare, M.J.</creatorcontrib><creatorcontrib>Thomas, Dilys M.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Clinica chimica acta</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Moss, D.W.</au><au>Shakespeare, M.J.</au><au>Thomas, Dilys M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Observations on the heat-stability of alkaline phosphatase isoenzymes in serum</atitle><jtitle>Clinica chimica acta</jtitle><addtitle>Clin Chim Acta</addtitle><date>1972-08</date><risdate>1972</risdate><volume>40</volume><issue>1</issue><spage>35</spage><epage>41</epage><pages>35-41</pages><issn>0009-8981</issn><eissn>1873-3492</eissn><abstract>Serum specimens which apparently contain the same isoenzyme of alkaline phosphatase nevertheless show wide variations in the stabilities of their phosphatase activities on heating at 56°. A number of factors which may influence the stability of bone and liver phosphatases in serum have been studied and their contributions to the observed variability of serum phosphatase heat-stabilities have been assessed. Differences between specimens in protein or urea content do not appear to be significant in this respect, but differences in pH may cause variability in enzyme heat-stability. When differences between serum samples in characteristics such as pH have been taken into account, however, the range of variation which still remains suggests that a particular phosphatase isoenzyme entering the circulation in different individuals and in various diseases may not have constant stability properties.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>5056641</pmid><doi>10.1016/0009-8981(72)90248-3</doi><tpages>7</tpages></addata></record> |
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| ispartof | Clinica chimica acta, 1972-08, Vol.40 (1), p.35-41 |
| issn | 0009-8981 1873-3492 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Alkaline Phosphatase - blood Autoanalysis Bone and Bones - enzymology Drug Stability Electrophoresis, Starch Gel Hot Temperature Humans Hydrogen-Ion Concentration Isoenzymes - blood Liver - enzymology Osmolar Concentration Serum Albumin, Bovine - analysis Urea - blood |
| title | Observations on the heat-stability of alkaline phosphatase isoenzymes in serum |
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