Quantitative Spectrodensitometry of Silica Gel Thin Layers Impregnated With Sulfuric Acid

Impregnating silica gel layers with sulfuric acid prior to development of the chromatogram overcomes problems of spraying. Silica gel G plates (250 µ) were dipped for 30 sec in a solution of 4% sulfuric acid in methanol (v/v). After drying, chromatography can be carried out in the usual manner. Sepa...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Journal of chromatographic science 1972-08, Vol.10 (8), p.490-493
Hauptverfasser:	Touchstone, J C, Murawec, T, Kasparow, M, Wortmann, W
Format:	Artikel
Sprache:	eng
Schlagworte:	Cholesterol - analysis Chromatography, Thin Layer Densitometry Estradiol - analysis Estriol - analysis Gels Hydrocortisone - analysis Silicon Spectrum Analysis Steroids - analysis Sulfuric Acids Testosterone - analysis
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

container_end_page	493
container_issue	8
container_start_page	490
container_title	Journal of chromatographic science
container_volume	10
creator	Touchstone, J C Murawec, T Kasparow, M Wortmann, W
description	Impregnating silica gel layers with sulfuric acid prior to development of the chromatogram overcomes problems of spraying. Silica gel G plates (250 µ) were dipped for 30 sec in a solution of 4% sulfuric acid in methanol (v/v). After drying, chromatography can be carried out in the usual manner. Separations were comparable to those obtained with normal plates but using solvent systems of lower polarity. After development the plates were heated in an oven at 140° for 20 min for complete charring. At 120° for 10 min various colors developed. Using a Schoeffel Model 3000 double beam spectrodensitometer linear calibration curves were obtained for steroids and fatty acids in submicrogram ranges.
doi_str_mv	10.1093/chromsci/10.8.490
format	Article
fullrecord	<record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_81560791</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>81560791</sourcerecordid><originalsourceid>FETCH-LOGICAL-c334t-270e59bd3e5a2a5d84d314882ebf93aa2a1975adb12901810b51b367ad00d8503</originalsourceid><addsrcrecordid>eNo9kE1P2zAYxy3E1HXAB-CA5BO3FDuOE_vIYINKZQgVaOFiOfaT1VteOttB9NuTqh2nR8__7fBD6JSSCSWSXZiV75pg3MUgiEkmyQEaU5nxJGVyeYjGhKQ0kXnOv6JvIfzZvlTwERpxwlOZ8zF6eeh1G13U0b0Bnq_BRN9ZaIOLXQPRb3BX4bmrndH4Bmr8uHItnukN-ICnzdrD71ZHsHjh4grP-7rqvTP40jh7jL5Uug5wsr9H6Onnj8er22R2fzO9upwlhrEsJmlBgMvSMuA61dyKzDKaCZFCWUmmB43Kgmtb0lQSKigpOS1ZXmhLiBWcsCN0vttd--5fDyGqxgUDda1b6PqgBOU5KSQdgnQXNL4LwUOl1t412m8UJWqLU_3HuRWEGnAOnbP9eF82YD8be36Dn-x8FyK8f9ra_1V5wQqubpev6tfz6_X87jtVC_YBC0SC1w</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>81560791</pqid></control><display><type>article</type><title>Quantitative Spectrodensitometry of Silica Gel Thin Layers Impregnated With Sulfuric Acid</title><source>MEDLINE</source><source>Oxford University Press Journals Digital Archive Legacy</source><creator>Touchstone, J C ; Murawec, T ; Kasparow, M ; Wortmann, W</creator><creatorcontrib>Touchstone, J C ; Murawec, T ; Kasparow, M ; Wortmann, W</creatorcontrib><description>Impregnating silica gel layers with sulfuric acid prior to development of the chromatogram overcomes problems of spraying. Silica gel G plates (250 µ) were dipped for 30 sec in a solution of 4% sulfuric acid in methanol (v/v). After drying, chromatography can be carried out in the usual manner. Separations were comparable to those obtained with normal plates but using solvent systems of lower polarity. After development the plates were heated in an oven at 140° for 20 min for complete charring. At 120° for 10 min various colors developed. Using a Schoeffel Model 3000 double beam spectrodensitometer linear calibration curves were obtained for steroids and fatty acids in submicrogram ranges.</description><identifier>ISSN: 0021-9665</identifier><identifier>EISSN: 1945-239X</identifier><identifier>DOI: 10.1093/chromsci/10.8.490</identifier><identifier>PMID: 5052965</identifier><language>eng</language><publisher>United States: Oxford University Press</publisher><subject>Cholesterol - analysis ; Chromatography, Thin Layer ; Densitometry ; Estradiol - analysis ; Estriol - analysis ; Gels ; Hydrocortisone - analysis ; Silicon ; Spectrum Analysis ; Steroids - analysis ; Sulfuric Acids ; Testosterone - analysis</subject><ispartof>Journal of chromatographic science, 1972-08, Vol.10 (8), p.490-493</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c334t-270e59bd3e5a2a5d84d314882ebf93aa2a1975adb12901810b51b367ad00d8503</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/5052965$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Touchstone, J C</creatorcontrib><creatorcontrib>Murawec, T</creatorcontrib><creatorcontrib>Kasparow, M</creatorcontrib><creatorcontrib>Wortmann, W</creatorcontrib><title>Quantitative Spectrodensitometry of Silica Gel Thin Layers Impregnated With Sulfuric Acid</title><title>Journal of chromatographic science</title><addtitle>Journal of Chromatographic Science</addtitle><description>Impregnating silica gel layers with sulfuric acid prior to development of the chromatogram overcomes problems of spraying. Silica gel G plates (250 µ) were dipped for 30 sec in a solution of 4% sulfuric acid in methanol (v/v). After drying, chromatography can be carried out in the usual manner. Separations were comparable to those obtained with normal plates but using solvent systems of lower polarity. After development the plates were heated in an oven at 140° for 20 min for complete charring. At 120° for 10 min various colors developed. Using a Schoeffel Model 3000 double beam spectrodensitometer linear calibration curves were obtained for steroids and fatty acids in submicrogram ranges.</description><subject>Cholesterol - analysis</subject><subject>Chromatography, Thin Layer</subject><subject>Densitometry</subject><subject>Estradiol - analysis</subject><subject>Estriol - analysis</subject><subject>Gels</subject><subject>Hydrocortisone - analysis</subject><subject>Silicon</subject><subject>Spectrum Analysis</subject><subject>Steroids - analysis</subject><subject>Sulfuric Acids</subject><subject>Testosterone - analysis</subject><issn>0021-9665</issn><issn>1945-239X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1972</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kE1P2zAYxy3E1HXAB-CA5BO3FDuOE_vIYINKZQgVaOFiOfaT1VteOttB9NuTqh2nR8__7fBD6JSSCSWSXZiV75pg3MUgiEkmyQEaU5nxJGVyeYjGhKQ0kXnOv6JvIfzZvlTwERpxwlOZ8zF6eeh1G13U0b0Bnq_BRN9ZaIOLXQPRb3BX4bmrndH4Bmr8uHItnukN-ICnzdrD71ZHsHjh4grP-7rqvTP40jh7jL5Uug5wsr9H6Onnj8er22R2fzO9upwlhrEsJmlBgMvSMuA61dyKzDKaCZFCWUmmB43Kgmtb0lQSKigpOS1ZXmhLiBWcsCN0vttd--5fDyGqxgUDda1b6PqgBOU5KSQdgnQXNL4LwUOl1t412m8UJWqLU_3HuRWEGnAOnbP9eF82YD8be36Dn-x8FyK8f9ra_1V5wQqubpev6tfz6_X87jtVC_YBC0SC1w</recordid><startdate>197208</startdate><enddate>197208</enddate><creator>Touchstone, J C</creator><creator>Murawec, T</creator><creator>Kasparow, M</creator><creator>Wortmann, W</creator><general>Oxford University Press</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>197208</creationdate><title>Quantitative Spectrodensitometry of Silica Gel Thin Layers Impregnated With Sulfuric Acid</title><author>Touchstone, J C ; Murawec, T ; Kasparow, M ; Wortmann, W</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c334t-270e59bd3e5a2a5d84d314882ebf93aa2a1975adb12901810b51b367ad00d8503</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1972</creationdate><topic>Cholesterol - analysis</topic><topic>Chromatography, Thin Layer</topic><topic>Densitometry</topic><topic>Estradiol - analysis</topic><topic>Estriol - analysis</topic><topic>Gels</topic><topic>Hydrocortisone - analysis</topic><topic>Silicon</topic><topic>Spectrum Analysis</topic><topic>Steroids - analysis</topic><topic>Sulfuric Acids</topic><topic>Testosterone - analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Touchstone, J C</creatorcontrib><creatorcontrib>Murawec, T</creatorcontrib><creatorcontrib>Kasparow, M</creatorcontrib><creatorcontrib>Wortmann, W</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of chromatographic science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Touchstone, J C</au><au>Murawec, T</au><au>Kasparow, M</au><au>Wortmann, W</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Quantitative Spectrodensitometry of Silica Gel Thin Layers Impregnated With Sulfuric Acid</atitle><jtitle>Journal of chromatographic science</jtitle><addtitle>Journal of Chromatographic Science</addtitle><date>1972-08</date><risdate>1972</risdate><volume>10</volume><issue>8</issue><spage>490</spage><epage>493</epage><pages>490-493</pages><issn>0021-9665</issn><eissn>1945-239X</eissn><abstract>Impregnating silica gel layers with sulfuric acid prior to development of the chromatogram overcomes problems of spraying. Silica gel G plates (250 µ) were dipped for 30 sec in a solution of 4% sulfuric acid in methanol (v/v). After drying, chromatography can be carried out in the usual manner. Separations were comparable to those obtained with normal plates but using solvent systems of lower polarity. After development the plates were heated in an oven at 140° for 20 min for complete charring. At 120° for 10 min various colors developed. Using a Schoeffel Model 3000 double beam spectrodensitometer linear calibration curves were obtained for steroids and fatty acids in submicrogram ranges.</abstract><cop>United States</cop><pub>Oxford University Press</pub><pmid>5052965</pmid><doi>10.1093/chromsci/10.8.490</doi><tpages>4</tpages></addata></record>
fulltext	fulltext
identifier	ISSN: 0021-9665
ispartof	Journal of chromatographic science, 1972-08, Vol.10 (8), p.490-493
issn	0021-9665 1945-239X
language	eng
recordid	cdi_proquest_miscellaneous_81560791
source	MEDLINE; Oxford University Press Journals Digital Archive Legacy
subjects	Cholesterol - analysis Chromatography, Thin Layer Densitometry Estradiol - analysis Estriol - analysis Gels Hydrocortisone - analysis Silicon Spectrum Analysis Steroids - analysis Sulfuric Acids Testosterone - analysis
title	Quantitative Spectrodensitometry of Silica Gel Thin Layers Impregnated With Sulfuric Acid
url	https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-27T13%3A42%3A49IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Quantitative%20Spectrodensitometry%20of%20Silica%20Gel%20Thin%20Layers%20Impregnated%20With%20Sulfuric%20Acid&rft.jtitle=Journal%20of%20chromatographic%20science&rft.au=Touchstone,%20J%20C&rft.date=1972-08&rft.volume=10&rft.issue=8&rft.spage=490&rft.epage=493&rft.pages=490-493&rft.issn=0021-9665&rft.eissn=1945-239X&rft_id=info:doi/10.1093/chromsci/10.8.490&rft_dat=%3Cproquest_cross%3E81560791%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=81560791&rft_id=info:pmid/5052965&rfr_iscdi=true