Measurement of mechanical tractions exerted by cells in three-dimensional matrices

Quantitative measurements of cell-generated forces have heretofore required that cells be cultured on two-dimensional substrates. We describe a technique to quantitatively measure three-dimensional traction forces exerted by cells fully encapsulated in well-defined elastic hydrogel matrices. Using t...

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Veröffentlicht in:	Nature methods 2010-12, Vol.7 (12), p.969-971
Hauptverfasser:	Chen, Christopher S, Legant, Wesley R, Miller, Jordan S, Blakely, Brandon L, Cohen, Daniel M, Genin, Guy M
Format:	Artikel
Sprache:	eng
Schlagworte:	3-D graphics 3T3 Cells - cytology 3T3 Cells - drug effects 3T3 Cells - physiology 631/1647/2204 631/57 631/80 Animals Bioinformatics Biological Microscopy Biological Techniques Biomedical and Life Sciences Biomedical Engineering/Biotechnology brief-communication Cell Adhesion - physiology Cell Culture Techniques - methods Cell Division Cell Movement - physiology Cells Cells, Cultured - cytology Cells, Cultured - physiology Cellular signal transduction Culture Media Elastic Modulus - physiology Extracellular Matrix - physiology Fibroblasts - cytology Fibroblasts - physiology Green Fluorescent Proteins - genetics Hepatocyte Growth Factor - pharmacology Humans Hydrogel, Polyethylene Glycol Dimethacrylate Life Sciences Mechanical properties Mice Physiological aspects Proteomics Recombinant Proteins - pharmacology Stress, Mechanical Substrates (Biochemistry) Traction
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