Measurement of mechanical tractions exerted by cells in three-dimensional matrices
Tracking the displacement of fluorescent beads surrounding a cell embedded in a hydrogel matrix allows quantitative measurement of the three-dimensional traction forces exerted by the cell. Quantitative measurements of cell-generated forces have heretofore required that cells be cultured on two-dime...
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| Veröffentlicht in: | Nature methods 2010-12, Vol.7 (12), p.969-971 |
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| creator | Legant, Wesley R Miller, Jordan S Blakely, Brandon L Cohen, Daniel M Genin, Guy M Chen, Christopher S |
| description | Tracking the displacement of fluorescent beads surrounding a cell embedded in a hydrogel matrix allows quantitative measurement of the three-dimensional traction forces exerted by the cell.
Quantitative measurements of cell-generated forces have heretofore required that cells be cultured on two-dimensional substrates. We describe a technique to quantitatively measure three-dimensional traction forces exerted by cells fully encapsulated in well-defined elastic hydrogel matrices. Using this approach we measured traction forces for several cell types in various contexts and revealed patterns of force generation attributable to morphologically distinct regions of cells as they extend into the surrounding matrix. |
| doi_str_mv | 10.1038/nmeth.1531 |
| format | Article |
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Quantitative measurements of cell-generated forces have heretofore required that cells be cultured on two-dimensional substrates. We describe a technique to quantitatively measure three-dimensional traction forces exerted by cells fully encapsulated in well-defined elastic hydrogel matrices. Using this approach we measured traction forces for several cell types in various contexts and revealed patterns of force generation attributable to morphologically distinct regions of cells as they extend into the surrounding matrix.</description><identifier>ISSN: 1548-7091</identifier><identifier>EISSN: 1548-7105</identifier><identifier>DOI: 10.1038/nmeth.1531</identifier><identifier>PMID: 21076420</identifier><language>eng</language><publisher>New York: Nature Publishing Group US</publisher><subject>3-D graphics ; 3T3 Cells - cytology ; 3T3 Cells - drug effects ; 3T3 Cells - physiology ; 631/1647/2204 ; 631/57 ; 631/80 ; Animals ; Bioinformatics ; Biological Microscopy ; Biological Techniques ; Biomedical and Life Sciences ; Biomedical Engineering/Biotechnology ; brief-communication ; Cell Adhesion - physiology ; Cell Culture Techniques - methods ; Cell Division ; Cell Movement - physiology ; Cells ; Cells, Cultured - cytology ; Cells, Cultured - physiology ; Cellular signal transduction ; Culture Media ; Elastic Modulus - physiology ; Extracellular Matrix - physiology ; Fibroblasts - cytology ; Fibroblasts - physiology ; Green Fluorescent Proteins - genetics ; Hepatocyte Growth Factor - pharmacology ; Humans ; Hydrogel, Polyethylene Glycol Dimethacrylate ; Life Sciences ; Mechanical properties ; Mice ; Physiological aspects ; Proteomics ; Recombinant Proteins - pharmacology ; Stress, Mechanical ; Substrates (Biochemistry) ; Traction</subject><ispartof>Nature methods, 2010-12, Vol.7 (12), p.969-971</ispartof><rights>Springer Nature America, Inc. 2010</rights><rights>COPYRIGHT 2010 Nature Publishing Group</rights><rights>Copyright Nature Publishing Group Dec 2010</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c559t-a9a098d27d8aa2be84274b3ef8bb3c9f54622c4d222050490df57968a9c2f2bf3</citedby><cites>FETCH-LOGICAL-c559t-a9a098d27d8aa2be84274b3ef8bb3c9f54622c4d222050490df57968a9c2f2bf3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1038/nmeth.1531$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1038/nmeth.1531$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21076420$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Legant, Wesley R</creatorcontrib><creatorcontrib>Miller, Jordan S</creatorcontrib><creatorcontrib>Blakely, Brandon L</creatorcontrib><creatorcontrib>Cohen, Daniel M</creatorcontrib><creatorcontrib>Genin, Guy M</creatorcontrib><creatorcontrib>Chen, Christopher S</creatorcontrib><title>Measurement of mechanical tractions exerted by cells in three-dimensional matrices</title><title>Nature methods</title><addtitle>Nat Methods</addtitle><addtitle>Nat Methods</addtitle><description>Tracking the displacement of fluorescent beads surrounding a cell embedded in a hydrogel matrix allows quantitative measurement of the three-dimensional traction forces exerted by the cell.
Quantitative measurements of cell-generated forces have heretofore required that cells be cultured on two-dimensional substrates. We describe a technique to quantitatively measure three-dimensional traction forces exerted by cells fully encapsulated in well-defined elastic hydrogel matrices. Using this approach we measured traction forces for several cell types in various contexts and revealed patterns of force generation attributable to morphologically distinct regions of cells as they extend into the surrounding matrix.</description><subject>3-D graphics</subject><subject>3T3 Cells - cytology</subject><subject>3T3 Cells - drug effects</subject><subject>3T3 Cells - physiology</subject><subject>631/1647/2204</subject><subject>631/57</subject><subject>631/80</subject><subject>Animals</subject><subject>Bioinformatics</subject><subject>Biological Microscopy</subject><subject>Biological Techniques</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedical Engineering/Biotechnology</subject><subject>brief-communication</subject><subject>Cell Adhesion - physiology</subject><subject>Cell Culture Techniques - methods</subject><subject>Cell Division</subject><subject>Cell Movement - physiology</subject><subject>Cells</subject><subject>Cells, Cultured - cytology</subject><subject>Cells, Cultured - 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Academic</collection><jtitle>Nature methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Legant, Wesley R</au><au>Miller, Jordan S</au><au>Blakely, Brandon L</au><au>Cohen, Daniel M</au><au>Genin, Guy M</au><au>Chen, Christopher S</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Measurement of mechanical tractions exerted by cells in three-dimensional matrices</atitle><jtitle>Nature methods</jtitle><stitle>Nat Methods</stitle><addtitle>Nat Methods</addtitle><date>2010-12-01</date><risdate>2010</risdate><volume>7</volume><issue>12</issue><spage>969</spage><epage>971</epage><pages>969-971</pages><issn>1548-7091</issn><eissn>1548-7105</eissn><abstract>Tracking the displacement of fluorescent beads surrounding a cell embedded in a hydrogel matrix allows quantitative measurement of the three-dimensional traction forces exerted by the cell.
Quantitative measurements of cell-generated forces have heretofore required that cells be cultured on two-dimensional substrates. We describe a technique to quantitatively measure three-dimensional traction forces exerted by cells fully encapsulated in well-defined elastic hydrogel matrices. Using this approach we measured traction forces for several cell types in various contexts and revealed patterns of force generation attributable to morphologically distinct regions of cells as they extend into the surrounding matrix.</abstract><cop>New York</cop><pub>Nature Publishing Group US</pub><pmid>21076420</pmid><doi>10.1038/nmeth.1531</doi><tpages>3</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | 3-D graphics 3T3 Cells - cytology 3T3 Cells - drug effects 3T3 Cells - physiology 631/1647/2204 631/57 631/80 Animals Bioinformatics Biological Microscopy Biological Techniques Biomedical and Life Sciences Biomedical Engineering/Biotechnology brief-communication Cell Adhesion - physiology Cell Culture Techniques - methods Cell Division Cell Movement - physiology Cells Cells, Cultured - cytology Cells, Cultured - physiology Cellular signal transduction Culture Media Elastic Modulus - physiology Extracellular Matrix - physiology Fibroblasts - cytology Fibroblasts - physiology Green Fluorescent Proteins - genetics Hepatocyte Growth Factor - pharmacology Humans Hydrogel, Polyethylene Glycol Dimethacrylate Life Sciences Mechanical properties Mice Physiological aspects Proteomics Recombinant Proteins - pharmacology Stress, Mechanical Substrates (Biochemistry) Traction |
| title | Measurement of mechanical tractions exerted by cells in three-dimensional matrices |
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