Evaluation of Data Analysis Strategies for Improved Mass Spectrometry-Based Phosphoproteomics

Here we describe a set of enhanced data processing and filtering methods to improve significance and coverage of phosphopeptide identifications by mass spectrometry. We demonstrate that for samples of limited complexity, spectra-based estimation of false discovery rates will lead to overprediction o...

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Veröffentlicht in:	Analytical chemistry (Washington) 2010-12, Vol.82 (23), p.9843-9849
Hauptverfasser:	Savitski, Mikhail M, Scholten, Arjen, Sweetman, Gavain, Mathieson, Toby, Bantscheff, Marcus
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Analytical chemistry Biological and medical sciences Cell Line, Tumor Chemistry Chromatography Chromatography, High Pressure Liquid - methods Diverse techniques Exact sciences and technology Fundamental and applied biological sciences. Psychology Humans Mass spectrometry Mass Spectrometry - methods Molecular and cellular biology Molecular Sequence Data Peptides Phosphopeptides - chemistry Phosphorylation Proteomics - methods Software Spectrometric and optical methods
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