Incubated Protein Reduction and Digestion on an Electrowetting-on-Dielectric Digital Microfluidic Chip for MALDI-MS

Localized heating of droplets on an electrowetting-on-dielectric (EWOD) chip has been implemented and shown to accelerate trypsin digestion reaction rates, sample drying, and matrix crystallization for matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS). Achieving this involved...

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Veröffentlicht in:	Analytical chemistry (Washington) 2010-12, Vol.82 (23), p.9932-9937
Hauptverfasser:	Nelson, Wyatt C, Peng, Ivory, Lee, Geun-An, Loo, Joseph A, Garrell, Robin L, “CJ” Kim, Chang-Jin
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical chemistry Chemical reactions Chemistry Cytochromes c - metabolism Dimethyl Sulfoxide - chemistry Drying agents Electrodes Electrowetting - methods Exact sciences and technology Heating Insulin - chemistry Insulin - metabolism Mass spectrometry Microfluidic Analytical Techniques - methods Oxidation-Reduction Peptides Spectrometric and optical methods Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods Temperature Trypsin - metabolism
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