Poloxamer 188 supplemented culture medium increases the vitality of Caco-2 cells after subcultivation and freeze/thaw cycles

Cryostorage media containing 1% of the non-ionic surfactant Poloxamer 188 provided full recovery of mammalian cells (Gonzalez Hernandez, 2006), but its effects during thawing of cryostored cells and proteolytic subcultivation are still unknown. In this study, the proliferation and viability of pre-c...

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Veröffentlicht in:	ALTEX, alternatives to animal experimentation alternatives to animal experimentation, 2010, Vol.27 (3), p.191-197
Hauptverfasser:	Karleta, Vera, Andrlik, Isabella, Braunmüller, Susanne, Franke, Thomas, Wirth, Michael, Gabor, Franz
Format:	Artikel
Sprache:	eng
Schlagworte:	Caco-2 Cells Cell Culture Techniques Cell Membrane Cell Proliferation - drug effects Cell Survival - drug effects Culture Media - chemistry Culture Media - pharmacology Freezing Humans Poloxamer - chemistry
Online-Zugang:	Volltext
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