Purification and characterization of a laccase from the white-rot fungus Trametes multicolor
The wood-degrading fungus Trametes multicolor secretes several laccase isoforms when grown on a simple medium containing copper in the millimolar range for stimulating laccase synthesis. The main isoenzyme laccase II was purified to apparent homogeneity from the culture supernatant by using anion-ex...
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| Veröffentlicht in: | Applied biochemistry and biotechnology 2002, Vol.98-100 (1-9), p.497-507 |
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| creator | LEITNER, Christian HESS, Johann GALHAUP, Christiane LUDWIG, Roland NIDETZKY, Bernd KULBE, Klaus D HALTRICH, Dietmar |
| description | The wood-degrading fungus Trametes multicolor secretes several laccase isoforms when grown on a simple medium containing copper in the millimolar range for stimulating laccase synthesis. The main isoenzyme laccase II was purified to apparent homogeneity from the culture supernatant by using anion-exchange chromatography and gel filtration. Laccase II is a monomeric glycoprotein with a molecular mass of 63 kDa as determined by sodium dodecylsulfate polyacrylamide gel electrophoresis, contains 18% glycosylation, and has a pI of 3.0. It oxidizes a variety of phenolic substrates as well as ferrocyanide and iodide. The pH optimum depends on the substrate employed and shows a bell-shaped pH activity profile with an optimum of 4.0 to 5.0 for the phenolic substrates, while the nonphenolic substrates ferrocyanide and 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonate) show a monotonic pH profile with a rate decreasing with increasing pH. |
| doi_str_mv | 10.1385/abab:98-100:1-9:497 |
| format | Article |
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The main isoenzyme laccase II was purified to apparent homogeneity from the culture supernatant by using anion-exchange chromatography and gel filtration. Laccase II is a monomeric glycoprotein with a molecular mass of 63 kDa as determined by sodium dodecylsulfate polyacrylamide gel electrophoresis, contains 18% glycosylation, and has a pI of 3.0. It oxidizes a variety of phenolic substrates as well as ferrocyanide and iodide. The pH optimum depends on the substrate employed and shows a bell-shaped pH activity profile with an optimum of 4.0 to 5.0 for the phenolic substrates, while the nonphenolic substrates ferrocyanide and 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonate) show a monotonic pH profile with a rate decreasing with increasing pH.</description><identifier>ISSN: 0273-2289</identifier><identifier>EISSN: 1559-0291</identifier><identifier>EISSN: 0273-2289</identifier><identifier>DOI: 10.1385/abab:98-100:1-9:497</identifier><identifier>PMID: 12018276</identifier><identifier>CODEN: ABIBDL</identifier><language>eng</language><publisher>Heidelberg: Springer</publisher><subject>Anion exchanging ; Anion-exchange chromatography ; Basidiomycota - enzymology ; Basidiomycota - growth & development ; Biological and medical sciences ; Biology of microorganisms of confirmed or potential industrial interest ; Biotechnology ; Electrophoresis ; Enzymes ; Ferrocyanide ; Fundamental and applied biological sciences. 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Psychology</topic><topic>Gel electrophoresis</topic><topic>Gel filtration</topic><topic>Glycoproteins</topic><topic>Glycosylation</topic><topic>Homogeneity</topic><topic>Hydrogen-Ion Concentration</topic><topic>Iodides</topic><topic>Iron cyanides</topic><topic>Isoenzymes - isolation & purification</topic><topic>Isoenzymes - metabolism</topic><topic>Isoforms</topic><topic>Kinetics</topic><topic>Laccase</topic><topic>Miscellaneous</topic><topic>Mission oriented research</topic><topic>Molecular Weight</topic><topic>Oxidoreductases - isolation & purification</topic><topic>Oxidoreductases - metabolism</topic><topic>pH effects</topic><topic>Phenolic compounds</topic><topic>Phenols</topic><topic>Polyacrylamide</topic><topic>Substrate Specificity</topic><topic>Substrates</topic><topic>Thermodynamics</topic><topic>Trametes multicolor</topic><topic>White rot</topic><topic>White rot fungi</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>LEITNER, Christian</creatorcontrib><creatorcontrib>HESS, Johann</creatorcontrib><creatorcontrib>GALHAUP, Christiane</creatorcontrib><creatorcontrib>LUDWIG, Roland</creatorcontrib><creatorcontrib>NIDETZKY, Bernd</creatorcontrib><creatorcontrib>KULBE, Klaus D</creatorcontrib><creatorcontrib>HALTRICH, Dietmar</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Environment Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><collection>Environment Abstracts</collection><collection>MEDLINE - Academic</collection><collection>Biotechnology Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><jtitle>Applied biochemistry and biotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>LEITNER, Christian</au><au>HESS, Johann</au><au>GALHAUP, Christiane</au><au>LUDWIG, Roland</au><au>NIDETZKY, Bernd</au><au>KULBE, Klaus D</au><au>HALTRICH, Dietmar</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Purification and characterization of a laccase from the white-rot fungus Trametes multicolor</atitle><jtitle>Applied biochemistry and biotechnology</jtitle><addtitle>Appl Biochem Biotechnol</addtitle><date>2002</date><risdate>2002</risdate><volume>98-100</volume><issue>1-9</issue><spage>497</spage><epage>507</epage><pages>497-507</pages><issn>0273-2289</issn><eissn>1559-0291</eissn><eissn>0273-2289</eissn><coden>ABIBDL</coden><abstract>The wood-degrading fungus Trametes multicolor secretes several laccase isoforms when grown on a simple medium containing copper in the millimolar range for stimulating laccase synthesis. The main isoenzyme laccase II was purified to apparent homogeneity from the culture supernatant by using anion-exchange chromatography and gel filtration. Laccase II is a monomeric glycoprotein with a molecular mass of 63 kDa as determined by sodium dodecylsulfate polyacrylamide gel electrophoresis, contains 18% glycosylation, and has a pI of 3.0. It oxidizes a variety of phenolic substrates as well as ferrocyanide and iodide. The pH optimum depends on the substrate employed and shows a bell-shaped pH activity profile with an optimum of 4.0 to 5.0 for the phenolic substrates, while the nonphenolic substrates ferrocyanide and 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonate) show a monotonic pH profile with a rate decreasing with increasing pH.</abstract><cop>Heidelberg</cop><pub>Springer</pub><pmid>12018276</pmid><doi>10.1385/abab:98-100:1-9:497</doi><tpages>11</tpages></addata></record> |
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| ispartof | Applied biochemistry and biotechnology, 2002, Vol.98-100 (1-9), p.497-507 |
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| subjects | Anion exchanging Anion-exchange chromatography Basidiomycota - enzymology Basidiomycota - growth & development Biological and medical sciences Biology of microorganisms of confirmed or potential industrial interest Biotechnology Electrophoresis Enzymes Ferrocyanide Fundamental and applied biological sciences. Psychology Gel electrophoresis Gel filtration Glycoproteins Glycosylation Homogeneity Hydrogen-Ion Concentration Iodides Iron cyanides Isoenzymes - isolation & purification Isoenzymes - metabolism Isoforms Kinetics Laccase Miscellaneous Mission oriented research Molecular Weight Oxidoreductases - isolation & purification Oxidoreductases - metabolism pH effects Phenolic compounds Phenols Polyacrylamide Substrate Specificity Substrates Thermodynamics Trametes multicolor White rot White rot fungi |
| title | Purification and characterization of a laccase from the white-rot fungus Trametes multicolor |
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