Intracellular release of recombinant green fluorescent protein (gfpuv) from Escherichia coli

The recombinant green fluorescent protein (gfp(uv)) was expressed by Escherichia coli DH5-alpha cells transformed with the plasmid pGFPuv. The gfp(uv) was selectively permeabilized from the cells in buffer solution (25 mM Tris-HCl, pH 8.0), after freezing (-70 degrees C for 15 h), by four freeze (-2...

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Veröffentlicht in:	Applied biochemistry and biotechnology 2002, Vol.98-100 (1-9), p.791-802
Hauptverfasser:	VESSONI PENNA, Thereza Christina, CHIARINI, E. B, MACHOSHVILI, Irene Alexeevna, ISHII, Marina, PESSOA, Adalberto JR
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Biological and medical sciences Biotechnology Buffer solutions Cell Membrane Permeability Cloning, Molecular - methods E coli Escherichia coli Escherichia coli - genetics Fluorescence Freeze-thawing Freezing Fundamental and applied biological sciences. Psychology Green fluorescent protein Green Fluorescent Proteins Kinetics Luminescent Proteins - biosynthesis Luminescent Proteins - genetics Methods. Procedures. Technologies Others Plasmids Proteins Recombinant Proteins - biosynthesis Sonication Various methods and equipments
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