Detection of universal variable fragments as markers for genetic studies: A novel technology for DNA fingerprinting
A novel DNA technology enables the detection of universal variable fragments (UVF), thus revealing genetic variation without a priori sequence information. The detection of UVF markers is based on two amplifications of genomic DNA with the polymerase chain reaction. In the first amplification, two s...
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| Veröffentlicht in: | Molecular biotechnology 2003-02, Vol.23 (2), p.117-125 |
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| creator | VAN HAERINGEN, W. A VAN DE GOOR, L. H. P PANNEMAN, H VAN LITH, H. A VAN HAERINGEN, H VAN ZUTPHEN, L. F. M |
| description | A novel DNA technology enables the detection of universal variable fragments (UVF), thus revealing genetic variation without a priori sequence information. The detection of UVF markers is based on two amplifications of genomic DNA with the polymerase chain reaction. In the first amplification, two short oligonucleotide primers produce a large number of fragments. One primer is based on a microsatellite sequence, whereas the second primer can have any sequence. In the second amplification, the length of the primers is increased in order to decrease the number of amplicons. This enables the selection of polymorphic fragments. Restriction digestion can be used to further increase the number of polymorphisms. Until now, we have demonstrated UVF in several different species. In addition, with the present study we have contributed to the linkage map of the rabbit by localizing 11 UVF markers on different linkage groups. Mendelian inheritance was shown in this linkage study through a backcross of two inbred rabbit strains. The power of the UVF technique is based on the selection for microsatellite variation in combination with the detection of single-nucleotide polymorphisms. UVF thus offers the possibility of increasing the clustering of markers and localizing genes in species for which sequence information is either not present or only scarcely present. |
| doi_str_mv | 10.1385/MB:23:2:117 |
| format | Article |
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A ; VAN DE GOOR, L. H. P ; PANNEMAN, H ; VAN LITH, H. A ; VAN HAERINGEN, H ; VAN ZUTPHEN, L. F. M</creator><creatorcontrib>VAN HAERINGEN, W. A ; VAN DE GOOR, L. H. P ; PANNEMAN, H ; VAN LITH, H. A ; VAN HAERINGEN, H ; VAN ZUTPHEN, L. F. M</creatorcontrib><description>A novel DNA technology enables the detection of universal variable fragments (UVF), thus revealing genetic variation without a priori sequence information. The detection of UVF markers is based on two amplifications of genomic DNA with the polymerase chain reaction. In the first amplification, two short oligonucleotide primers produce a large number of fragments. One primer is based on a microsatellite sequence, whereas the second primer can have any sequence. In the second amplification, the length of the primers is increased in order to decrease the number of amplicons. This enables the selection of polymorphic fragments. Restriction digestion can be used to further increase the number of polymorphisms. Until now, we have demonstrated UVF in several different species. In addition, with the present study we have contributed to the linkage map of the rabbit by localizing 11 UVF markers on different linkage groups. Mendelian inheritance was shown in this linkage study through a backcross of two inbred rabbit strains. The power of the UVF technique is based on the selection for microsatellite variation in combination with the detection of single-nucleotide polymorphisms. UVF thus offers the possibility of increasing the clustering of markers and localizing genes in species for which sequence information is either not present or only scarcely present.</description><identifier>ISSN: 1073-6085</identifier><identifier>EISSN: 1559-0305</identifier><identifier>EISSN: 1073-6085</identifier><identifier>DOI: 10.1385/MB:23:2:117</identifier><identifier>PMID: 12632696</identifier><identifier>CODEN: MLBOEO</identifier><language>eng</language><publisher>Totowa, NJ: Humana Press</publisher><subject>Animals ; Animals, Inbred Strains ; Biological and medical sciences ; Biotechnology ; Deoxyribonucleic acid ; DNA ; DNA Fingerprinting - methods ; DNA Primers - genetics ; Feasibility Studies ; Fundamental and applied biological sciences. Psychology ; Gene Expression Profiling - methods ; Genetic diversity ; Genetic engineering ; Genetic Linkage ; Genetic Markers - genetics ; Genetic technics ; Genetic Variation - genetics ; Genetics ; Genomics ; Horses - genetics ; In vitro gene amplification. Pcr technique ; Methods. Procedures. Technologies ; Microsatellite Repeats - genetics ; Polymerase Chain Reaction - methods ; Polymorphism, Genetic - genetics ; Rabbits - genetics ; Random Amplified Polymorphic DNA Technique - methods ; Reproducibility of Results ; Sensitivity and Specificity</subject><ispartof>Molecular biotechnology, 2003-02, Vol.23 (2), p.117-125</ispartof><rights>2003 INIST-CNRS</rights><rights>Humana Press Inc. 2003</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=14543925$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12632696$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>VAN HAERINGEN, W. A</creatorcontrib><creatorcontrib>VAN DE GOOR, L. H. P</creatorcontrib><creatorcontrib>PANNEMAN, H</creatorcontrib><creatorcontrib>VAN LITH, H. A</creatorcontrib><creatorcontrib>VAN HAERINGEN, H</creatorcontrib><creatorcontrib>VAN ZUTPHEN, L. F. M</creatorcontrib><title>Detection of universal variable fragments as markers for genetic studies: A novel technology for DNA fingerprinting</title><title>Molecular biotechnology</title><addtitle>Mol Biotechnol</addtitle><description>A novel DNA technology enables the detection of universal variable fragments (UVF), thus revealing genetic variation without a priori sequence information. The detection of UVF markers is based on two amplifications of genomic DNA with the polymerase chain reaction. In the first amplification, two short oligonucleotide primers produce a large number of fragments. One primer is based on a microsatellite sequence, whereas the second primer can have any sequence. In the second amplification, the length of the primers is increased in order to decrease the number of amplicons. This enables the selection of polymorphic fragments. Restriction digestion can be used to further increase the number of polymorphisms. Until now, we have demonstrated UVF in several different species. In addition, with the present study we have contributed to the linkage map of the rabbit by localizing 11 UVF markers on different linkage groups. Mendelian inheritance was shown in this linkage study through a backcross of two inbred rabbit strains. The power of the UVF technique is based on the selection for microsatellite variation in combination with the detection of single-nucleotide polymorphisms. UVF thus offers the possibility of increasing the clustering of markers and localizing genes in species for which sequence information is either not present or only scarcely present.</description><subject>Animals</subject><subject>Animals, Inbred Strains</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA Fingerprinting - methods</subject><subject>DNA Primers - genetics</subject><subject>Feasibility Studies</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression Profiling - methods</subject><subject>Genetic diversity</subject><subject>Genetic engineering</subject><subject>Genetic Linkage</subject><subject>Genetic Markers - genetics</subject><subject>Genetic technics</subject><subject>Genetic Variation - genetics</subject><subject>Genetics</subject><subject>Genomics</subject><subject>Horses - genetics</subject><subject>In vitro gene amplification. Pcr technique</subject><subject>Methods. Procedures. Technologies</subject><subject>Microsatellite Repeats - genetics</subject><subject>Polymerase Chain Reaction - methods</subject><subject>Polymorphism, Genetic - genetics</subject><subject>Rabbits - genetics</subject><subject>Random Amplified Polymorphic DNA Technique - methods</subject><subject>Reproducibility of Results</subject><subject>Sensitivity and Specificity</subject><issn>1073-6085</issn><issn>1559-0305</issn><issn>1073-6085</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNqF0U1rFTEUBuAgiq3VlXsJgnYho0nOJJO5u9sPP6DVja5DJpNcU-cmbTJzof_eU3uh4EJXOZCHF855CXnJ2XsOWn64PFkJWIkV590jcsil7BsGTD7GmXXQKKblAXlW6xVjgssWnpIDLhQI1atDUs_87N0cc6I50CXFnS_VTnRnS7TD5GkodrP1aa7UVrq15Rf-05AL3fjk5-honZcx-rqia5ryzk8U836mPOXN7R939nVNQ0wbX65LTDNOz8mTYKfqX-zfI_Lj4_n308_NxbdPX07XF40DBXMztrLnLYCyQjvFnFQjD84xZ0GHEYbQW-30IMLQdspK1YPtWnCDVWMA1wk4Isf3udcl3yy-zmYbq_PTZJPPSzUabwW9Unfy7T9lB0yrXur_Qq47JnQHCF__Ba_yUhKuawQozZApRO_ukSu51uKDwRPhjW8NZ-auW3N5gtwIg92ifrWPXIatHx_svkwEb_bAVmcnbC65WB9ci-X3QsJvTzasKA</recordid><startdate>20030201</startdate><enddate>20030201</enddate><creator>VAN HAERINGEN, W. 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A</au><au>VAN DE GOOR, L. H. P</au><au>PANNEMAN, H</au><au>VAN LITH, H. A</au><au>VAN HAERINGEN, H</au><au>VAN ZUTPHEN, L. F. M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Detection of universal variable fragments as markers for genetic studies: A novel technology for DNA fingerprinting</atitle><jtitle>Molecular biotechnology</jtitle><addtitle>Mol Biotechnol</addtitle><date>2003-02-01</date><risdate>2003</risdate><volume>23</volume><issue>2</issue><spage>117</spage><epage>125</epage><pages>117-125</pages><issn>1073-6085</issn><eissn>1559-0305</eissn><eissn>1073-6085</eissn><coden>MLBOEO</coden><abstract>A novel DNA technology enables the detection of universal variable fragments (UVF), thus revealing genetic variation without a priori sequence information. The detection of UVF markers is based on two amplifications of genomic DNA with the polymerase chain reaction. In the first amplification, two short oligonucleotide primers produce a large number of fragments. One primer is based on a microsatellite sequence, whereas the second primer can have any sequence. In the second amplification, the length of the primers is increased in order to decrease the number of amplicons. This enables the selection of polymorphic fragments. Restriction digestion can be used to further increase the number of polymorphisms. Until now, we have demonstrated UVF in several different species. In addition, with the present study we have contributed to the linkage map of the rabbit by localizing 11 UVF markers on different linkage groups. Mendelian inheritance was shown in this linkage study through a backcross of two inbred rabbit strains. The power of the UVF technique is based on the selection for microsatellite variation in combination with the detection of single-nucleotide polymorphisms. UVF thus offers the possibility of increasing the clustering of markers and localizing genes in species for which sequence information is either not present or only scarcely present.</abstract><cop>Totowa, NJ</cop><pub>Humana Press</pub><pmid>12632696</pmid><doi>10.1385/MB:23:2:117</doi><tpages>9</tpages></addata></record> |
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| ispartof | Molecular biotechnology, 2003-02, Vol.23 (2), p.117-125 |
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| subjects | Animals Animals, Inbred Strains Biological and medical sciences Biotechnology Deoxyribonucleic acid DNA DNA Fingerprinting - methods DNA Primers - genetics Feasibility Studies Fundamental and applied biological sciences. Psychology Gene Expression Profiling - methods Genetic diversity Genetic engineering Genetic Linkage Genetic Markers - genetics Genetic technics Genetic Variation - genetics Genetics Genomics Horses - genetics In vitro gene amplification. Pcr technique Methods. Procedures. Technologies Microsatellite Repeats - genetics Polymerase Chain Reaction - methods Polymorphism, Genetic - genetics Rabbits - genetics Random Amplified Polymorphic DNA Technique - methods Reproducibility of Results Sensitivity and Specificity |
| title | Detection of universal variable fragments as markers for genetic studies: A novel technology for DNA fingerprinting |
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