Antioxidant activity in vitro of the selenium-contained protein from the Se-enriched Bifidobacterium animalis 01
Several studies indicated that bifidobacteria possessed strong antioxidant activity. In present study, the antioxidant activities of Bifidobacterium animalis 01 proteins were evaluated using six assays, namely, linoleic acid preoxidation assay, erythrocyte hemolysis assay, 1,1-diphenyl-2-picrylhydra...
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| Veröffentlicht in: | Anaerobe 2010-08, Vol.16 (4), p.380-386 |
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| creator | Shen, Qian Zhang, Bowen Xu, Rihua Wang, Yang Ding, Xuelong Li, Pinglan |
| description | Several studies indicated that bifidobacteria possessed strong antioxidant activity. In present study, the antioxidant activities of
Bifidobacterium animalis 01 proteins were evaluated using six assays, namely, linoleic acid preoxidation assay, erythrocyte hemolysis assay, 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay, reducing power assay, hydroxyl (
OH) and superoxide radicals (
O
2
−) assays, in which the last two assays were measured by electron spin resonance (ESR). There were two kinds of
B.
animalis 01 proteins in this study, the regular
B.
animalis 01 protein (Pro-CK) and the
B.
animalis 01 selenium-contained protein (Pro-Se). Both Pro-CK and Pro-Se showed concentration dependent antioxidant activity in DPPH assay, reducing power assay and erythrocyte hemolysis assay. All results of six assays indicated that the antioxidant activity of the
B.
animalis 01 protein was improved remarkably after selenium was incorporated. The antioxidant activity of Pro-Se increased with the increase of selenium content in Pro-Se suggesting selenium played a positive role in enhancing the antioxidant activity of
B.
animalis 01 protein. Moreover, organic selenium was more effective than inorganic selenium on enhancing the hydroxyl radical scavenging ability of
B.
animalis 01 protein. |
| doi_str_mv | 10.1016/j.anaerobe.2010.06.006 |
| format | Article |
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Bifidobacterium animalis 01 proteins were evaluated using six assays, namely, linoleic acid preoxidation assay, erythrocyte hemolysis assay, 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay, reducing power assay, hydroxyl (
OH) and superoxide radicals (
O
2
−) assays, in which the last two assays were measured by electron spin resonance (ESR). There were two kinds of
B.
animalis 01 proteins in this study, the regular
B.
animalis 01 protein (Pro-CK) and the
B.
animalis 01 selenium-contained protein (Pro-Se). Both Pro-CK and Pro-Se showed concentration dependent antioxidant activity in DPPH assay, reducing power assay and erythrocyte hemolysis assay. All results of six assays indicated that the antioxidant activity of the
B.
animalis 01 protein was improved remarkably after selenium was incorporated. The antioxidant activity of Pro-Se increased with the increase of selenium content in Pro-Se suggesting selenium played a positive role in enhancing the antioxidant activity of
B.
animalis 01 protein. Moreover, organic selenium was more effective than inorganic selenium on enhancing the hydroxyl radical scavenging ability of
B.
animalis 01 protein.</description><identifier>ISSN: 1075-9964</identifier><identifier>EISSN: 1095-8274</identifier><identifier>DOI: 10.1016/j.anaerobe.2010.06.006</identifier><identifier>PMID: 20601030</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Antioxidant activity ; Antioxidants - isolation & purification ; Antioxidants - pharmacology ; Bacterial Proteins - isolation & purification ; Bacterial Proteins - pharmacology ; Bifidobacterium - chemistry ; Bifidobacterium animalis ; Biphenyl Compounds - metabolism ; Hemolysis ; Humans ; Hydroxyl Radical - metabolism ; Linoleic Acid - metabolism ; Oxidation-Reduction ; Picrates - metabolism ; Selenium ; Selenium - metabolism ; Selenium-contained protein ; Superoxides - metabolism</subject><ispartof>Anaerobe, 2010-08, Vol.16 (4), p.380-386</ispartof><rights>2010 Elsevier Ltd</rights><rights>Copyright (c) 2010 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c465t-f4aa3509d29729fda7a85c3cee9278eb41a24dd8a1e77e3d829d86f452251af3</citedby><cites>FETCH-LOGICAL-c465t-f4aa3509d29729fda7a85c3cee9278eb41a24dd8a1e77e3d829d86f452251af3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S1075996410000995$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20601030$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Shen, Qian</creatorcontrib><creatorcontrib>Zhang, Bowen</creatorcontrib><creatorcontrib>Xu, Rihua</creatorcontrib><creatorcontrib>Wang, Yang</creatorcontrib><creatorcontrib>Ding, Xuelong</creatorcontrib><creatorcontrib>Li, Pinglan</creatorcontrib><title>Antioxidant activity in vitro of the selenium-contained protein from the Se-enriched Bifidobacterium animalis 01</title><title>Anaerobe</title><addtitle>Anaerobe</addtitle><description>Several studies indicated that bifidobacteria possessed strong antioxidant activity. In present study, the antioxidant activities of
Bifidobacterium animalis 01 proteins were evaluated using six assays, namely, linoleic acid preoxidation assay, erythrocyte hemolysis assay, 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay, reducing power assay, hydroxyl (
OH) and superoxide radicals (
O
2
−) assays, in which the last two assays were measured by electron spin resonance (ESR). There were two kinds of
B.
animalis 01 proteins in this study, the regular
B.
animalis 01 protein (Pro-CK) and the
B.
animalis 01 selenium-contained protein (Pro-Se). Both Pro-CK and Pro-Se showed concentration dependent antioxidant activity in DPPH assay, reducing power assay and erythrocyte hemolysis assay. All results of six assays indicated that the antioxidant activity of the
B.
animalis 01 protein was improved remarkably after selenium was incorporated. The antioxidant activity of Pro-Se increased with the increase of selenium content in Pro-Se suggesting selenium played a positive role in enhancing the antioxidant activity of
B.
animalis 01 protein. Moreover, organic selenium was more effective than inorganic selenium on enhancing the hydroxyl radical scavenging ability of
B.
animalis 01 protein.</description><subject>Antioxidant activity</subject><subject>Antioxidants - isolation & purification</subject><subject>Antioxidants - pharmacology</subject><subject>Bacterial Proteins - isolation & purification</subject><subject>Bacterial Proteins - pharmacology</subject><subject>Bifidobacterium - chemistry</subject><subject>Bifidobacterium animalis</subject><subject>Biphenyl Compounds - metabolism</subject><subject>Hemolysis</subject><subject>Humans</subject><subject>Hydroxyl Radical - metabolism</subject><subject>Linoleic Acid - metabolism</subject><subject>Oxidation-Reduction</subject><subject>Picrates - metabolism</subject><subject>Selenium</subject><subject>Selenium - metabolism</subject><subject>Selenium-contained protein</subject><subject>Superoxides - metabolism</subject><issn>1075-9964</issn><issn>1095-8274</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1vVCEUhonR2Fr9Cw07V3cE7gUuO2tTP5ImLuyeMHBIz-ReGIFp7L-XcVq3XR0Cz3sO8BByydmGM64-7TYuOSh5CxvB-iZTG8bUK3LOmZHDLPT0-rjWcjBGTWfkXa07xjifpHxLzgRTPTSyc7K_Sg3zHwwuNep8wwdsjxQT7bVkmiNt90ArLJDwsA4-p-YwQaD7kht0Lpa8_mN-wQCpoL_vh18wYsjb3g9Kj1GXcHULVsr4e_ImuqXCh6d6Qe6-3txdfx9uf377cX11O_hJyTbEyblRMhOE0cLE4LSbpR89gBF6hu3EnZhCmB0HrWEMszBhVnGSQkju4nhBPp7a9nv-PkBtdsXqYVlcgnyoduZSjkaN84uk7pzRRopOqhPpS661QLT70t9VHi1n9mjF7uyzFXu0Ypmy3UoPXj6NOGxXCP9jzxo68PkEQP-RB4Riq0dIHgIW8M2GjC_N-Auj36Kz</recordid><startdate>20100801</startdate><enddate>20100801</enddate><creator>Shen, Qian</creator><creator>Zhang, Bowen</creator><creator>Xu, Rihua</creator><creator>Wang, Yang</creator><creator>Ding, Xuelong</creator><creator>Li, Pinglan</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QL</scope><scope>C1K</scope></search><sort><creationdate>20100801</creationdate><title>Antioxidant activity in vitro of the selenium-contained protein from the Se-enriched Bifidobacterium animalis 01</title><author>Shen, Qian ; Zhang, Bowen ; Xu, Rihua ; Wang, Yang ; Ding, Xuelong ; Li, Pinglan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c465t-f4aa3509d29729fda7a85c3cee9278eb41a24dd8a1e77e3d829d86f452251af3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Antioxidant activity</topic><topic>Antioxidants - isolation & purification</topic><topic>Antioxidants - pharmacology</topic><topic>Bacterial Proteins - isolation & purification</topic><topic>Bacterial Proteins - pharmacology</topic><topic>Bifidobacterium - chemistry</topic><topic>Bifidobacterium animalis</topic><topic>Biphenyl Compounds - metabolism</topic><topic>Hemolysis</topic><topic>Humans</topic><topic>Hydroxyl Radical - metabolism</topic><topic>Linoleic Acid - metabolism</topic><topic>Oxidation-Reduction</topic><topic>Picrates - metabolism</topic><topic>Selenium</topic><topic>Selenium - metabolism</topic><topic>Selenium-contained protein</topic><topic>Superoxides - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shen, Qian</creatorcontrib><creatorcontrib>Zhang, Bowen</creatorcontrib><creatorcontrib>Xu, Rihua</creatorcontrib><creatorcontrib>Wang, Yang</creatorcontrib><creatorcontrib>Ding, Xuelong</creatorcontrib><creatorcontrib>Li, Pinglan</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>Anaerobe</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shen, Qian</au><au>Zhang, Bowen</au><au>Xu, Rihua</au><au>Wang, Yang</au><au>Ding, Xuelong</au><au>Li, Pinglan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Antioxidant activity in vitro of the selenium-contained protein from the Se-enriched Bifidobacterium animalis 01</atitle><jtitle>Anaerobe</jtitle><addtitle>Anaerobe</addtitle><date>2010-08-01</date><risdate>2010</risdate><volume>16</volume><issue>4</issue><spage>380</spage><epage>386</epage><pages>380-386</pages><issn>1075-9964</issn><eissn>1095-8274</eissn><abstract>Several studies indicated that bifidobacteria possessed strong antioxidant activity. In present study, the antioxidant activities of
Bifidobacterium animalis 01 proteins were evaluated using six assays, namely, linoleic acid preoxidation assay, erythrocyte hemolysis assay, 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay, reducing power assay, hydroxyl (
OH) and superoxide radicals (
O
2
−) assays, in which the last two assays were measured by electron spin resonance (ESR). There were two kinds of
B.
animalis 01 proteins in this study, the regular
B.
animalis 01 protein (Pro-CK) and the
B.
animalis 01 selenium-contained protein (Pro-Se). Both Pro-CK and Pro-Se showed concentration dependent antioxidant activity in DPPH assay, reducing power assay and erythrocyte hemolysis assay. All results of six assays indicated that the antioxidant activity of the
B.
animalis 01 protein was improved remarkably after selenium was incorporated. The antioxidant activity of Pro-Se increased with the increase of selenium content in Pro-Se suggesting selenium played a positive role in enhancing the antioxidant activity of
B.
animalis 01 protein. Moreover, organic selenium was more effective than inorganic selenium on enhancing the hydroxyl radical scavenging ability of
B.
animalis 01 protein.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>20601030</pmid><doi>10.1016/j.anaerobe.2010.06.006</doi><tpages>7</tpages></addata></record> |
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| subjects | Antioxidant activity Antioxidants - isolation & purification Antioxidants - pharmacology Bacterial Proteins - isolation & purification Bacterial Proteins - pharmacology Bifidobacterium - chemistry Bifidobacterium animalis Biphenyl Compounds - metabolism Hemolysis Humans Hydroxyl Radical - metabolism Linoleic Acid - metabolism Oxidation-Reduction Picrates - metabolism Selenium Selenium - metabolism Selenium-contained protein Superoxides - metabolism |
| title | Antioxidant activity in vitro of the selenium-contained protein from the Se-enriched Bifidobacterium animalis 01 |
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