Detection of metallo-b-lactamase-encoding genes among clinical isolates of Pseudomonas aeruginosa in northwest of Iran
The prevalence of metallo-b-lactamase (MBL) production among 104 clinical isolates of Pseudomonas aeruginosa from northwest of Iran was investigated by phenotypic and polymerase chain reaction (PCR) methods. Thirty-nine (37.50%) of isolates were MBL positive by double-disk synergy test. Results of P...
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Veröffentlicht in: | Diagnostic microbiology and infectious disease 2010-11, Vol.68 (3), p.322-325 |
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creator | Yousefi, Saber Farajnia, Safar Nahaei, Mohammd Reza Akhi, Mohammad Taghi Ghotaslou, Reza Soroush, Mohammad Hossein Naghili, Behrooz Jazani, Nima Hosseini |
description | The prevalence of metallo-b-lactamase (MBL) production among 104 clinical isolates of Pseudomonas aeruginosa from northwest of Iran was investigated by phenotypic and polymerase chain reaction (PCR) methods. Thirty-nine (37.50%) of isolates were MBL positive by double-disk synergy test. Results of PCR revealed that 18 (17.31%) and 6 (5.77%) imipenem nonsusceptible isolates of P. aeruginosa carried bla VIM and bla IMP genes respectively, while 92.4% (62/67) of isolates contained class 1 integron gene. This is the first report of MBL-producing P. aeruginosa from northwest of Iran. |
doi_str_mv | 10.1016/j.diagmicrobio.2010.06.018 |
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Thirty-nine (37.50%) of isolates were MBL positive by double-disk synergy test. Results of PCR revealed that 18 (17.31%) and 6 (5.77%) imipenem nonsusceptible isolates of P. aeruginosa carried bla VIM and bla IMP genes respectively, while 92.4% (62/67) of isolates contained class 1 integron gene. 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title | Detection of metallo-b-lactamase-encoding genes among clinical isolates of Pseudomonas aeruginosa in northwest of Iran |
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