Electrochemical studies of cystine modified self-assembled monolayer for Escherichia coli detection
An electrochemical DNA biosensor based on cystine modified self-assembled monolayer (cys-SAM) onto gold electrode (AuE) has been fabricated for Escherichia coli ( E. coli) detection. This biosensing electrode has been characterized using scanning electron microscopy (SEM), FT-IR spectroscopy, cyclic...
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| Veröffentlicht in: | Thin solid films 2010-11, Vol.519 (3), p.1178-1183 |
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| container_title | Thin solid films |
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| creator | Pandey, Chandra Mouli Sumana, Gajjala Sood, K.N. Malhotra, B.D. |
| description | An electrochemical DNA biosensor based on cystine modified self-assembled monolayer (cys-SAM) onto gold electrode (AuE) has been fabricated for
Escherichia coli (
E. coli) detection. This biosensing electrode has been characterized using scanning electron microscopy (SEM), FT-IR spectroscopy, cyclic voltammetry (CV), differential pulse voltammetry (DPV) and electrochemical impedance spectroscopy (EIS). Under the optimum conditions, this DNA biosensor can be used to detect complementary target DNA concentration in the range of 1
×
10
−
6
M to 1
×
10
−
20
M within 60
s of hybridization time at 25
°C and has been found to be stable for about four months when stored at 4
°C. |
| doi_str_mv | 10.1016/j.tsf.2010.08.065 |
| format | Article |
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Escherichia coli (
E. coli) detection. This biosensing electrode has been characterized using scanning electron microscopy (SEM), FT-IR spectroscopy, cyclic voltammetry (CV), differential pulse voltammetry (DPV) and electrochemical impedance spectroscopy (EIS). Under the optimum conditions, this DNA biosensor can be used to detect complementary target DNA concentration in the range of 1
×
10
−
6
M to 1
×
10
−
20
M within 60
s of hybridization time at 25
°C and has been found to be stable for about four months when stored at 4
°C.</description><identifier>ISSN: 0040-6090</identifier><identifier>EISSN: 1879-2731</identifier><identifier>DOI: 10.1016/j.tsf.2010.08.065</identifier><identifier>CODEN: THSFAP</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Biosensors ; Condensed matter: structure, mechanical and thermal properties ; Cystine ; Electrochemical DNA biosensor ; Escherichia coli ; Exact sciences and technology ; Methylene blue ; Physics ; Self-assembled monolayer ; Structure and morphology; thickness ; Surfaces and interfaces; thin films and whiskers (structure and nonelectronic properties) ; Thin film structure and morphology</subject><ispartof>Thin solid films, 2010-11, Vol.519 (3), p.1178-1183</ispartof><rights>2010 Elsevier B.V.</rights><rights>2015 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c359t-e896ebf2cb54e7c56e814c68cc851a45da15b405959c648c2d7dc2089282da203</citedby><cites>FETCH-LOGICAL-c359t-e896ebf2cb54e7c56e814c68cc851a45da15b405959c648c2d7dc2089282da203</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.tsf.2010.08.065$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>309,310,314,780,784,789,790,3550,23930,23931,25140,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=23779431$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Pandey, Chandra Mouli</creatorcontrib><creatorcontrib>Sumana, Gajjala</creatorcontrib><creatorcontrib>Sood, K.N.</creatorcontrib><creatorcontrib>Malhotra, B.D.</creatorcontrib><title>Electrochemical studies of cystine modified self-assembled monolayer for Escherichia coli detection</title><title>Thin solid films</title><description>An electrochemical DNA biosensor based on cystine modified self-assembled monolayer (cys-SAM) onto gold electrode (AuE) has been fabricated for
Escherichia coli (
E. coli) detection. This biosensing electrode has been characterized using scanning electron microscopy (SEM), FT-IR spectroscopy, cyclic voltammetry (CV), differential pulse voltammetry (DPV) and electrochemical impedance spectroscopy (EIS). Under the optimum conditions, this DNA biosensor can be used to detect complementary target DNA concentration in the range of 1
×
10
−
6
M to 1
×
10
−
20
M within 60
s of hybridization time at 25
°C and has been found to be stable for about four months when stored at 4
°C.</description><subject>Biosensors</subject><subject>Condensed matter: structure, mechanical and thermal properties</subject><subject>Cystine</subject><subject>Electrochemical DNA biosensor</subject><subject>Escherichia coli</subject><subject>Exact sciences and technology</subject><subject>Methylene blue</subject><subject>Physics</subject><subject>Self-assembled monolayer</subject><subject>Structure and morphology; thickness</subject><subject>Surfaces and interfaces; thin films and whiskers (structure and nonelectronic properties)</subject><subject>Thin film structure and morphology</subject><issn>0040-6090</issn><issn>1879-2731</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><recordid>eNp9kMGKFDEQhoMoOK4-gLdcxFOPSTrpTvAky6wKC170HDKVCpsh3VlTPcK8vVlm8eipKPj-v6iPsfdS7KWQ06fTfqO0V6Lvwu7FZF6wnbSzG9Q8ypdsJ4QWwySceM3eEJ2EEFKpccfgUBC2VuEBlwyhcNrOMSPxmjhcaMsr8qXGnDJGTljSEIhwOZa-LnWtJVyw8VQbP1DvaBkecuBQS-YRt16d6_qWvUqhEL57njfs193h5-234f7H1--3X-4HGI3bBrRuwmNScDQaZzATWqlhsgDWyKBNDNIctTDOOJi0BRXnCEpYp6yKQYnxhn289j62-vuMtPklE2ApYcV6Jm-lMaPTeuykvJLQKlHD5B9bXkK7eCn8k09_8t2nf_LphfXdZ898eG4P1EWlFlbI9C-oxnl2epSd-3zlsL_6J2PzBBlXwJhb9-Fjzf-58hfQmYxG</recordid><startdate>20101130</startdate><enddate>20101130</enddate><creator>Pandey, Chandra Mouli</creator><creator>Sumana, Gajjala</creator><creator>Sood, K.N.</creator><creator>Malhotra, B.D.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>C1K</scope></search><sort><creationdate>20101130</creationdate><title>Electrochemical studies of cystine modified self-assembled monolayer for Escherichia coli detection</title><author>Pandey, Chandra Mouli ; Sumana, Gajjala ; Sood, K.N. ; Malhotra, B.D.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c359t-e896ebf2cb54e7c56e814c68cc851a45da15b405959c648c2d7dc2089282da203</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Biosensors</topic><topic>Condensed matter: structure, mechanical and thermal properties</topic><topic>Cystine</topic><topic>Electrochemical DNA biosensor</topic><topic>Escherichia coli</topic><topic>Exact sciences and technology</topic><topic>Methylene blue</topic><topic>Physics</topic><topic>Self-assembled monolayer</topic><topic>Structure and morphology; thickness</topic><topic>Surfaces and interfaces; thin films and whiskers (structure and nonelectronic properties)</topic><topic>Thin film structure and morphology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pandey, Chandra Mouli</creatorcontrib><creatorcontrib>Sumana, Gajjala</creatorcontrib><creatorcontrib>Sood, K.N.</creatorcontrib><creatorcontrib>Malhotra, B.D.</creatorcontrib><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>Thin solid films</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pandey, Chandra Mouli</au><au>Sumana, Gajjala</au><au>Sood, K.N.</au><au>Malhotra, B.D.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Electrochemical studies of cystine modified self-assembled monolayer for Escherichia coli detection</atitle><jtitle>Thin solid films</jtitle><date>2010-11-30</date><risdate>2010</risdate><volume>519</volume><issue>3</issue><spage>1178</spage><epage>1183</epage><pages>1178-1183</pages><issn>0040-6090</issn><eissn>1879-2731</eissn><coden>THSFAP</coden><abstract>An electrochemical DNA biosensor based on cystine modified self-assembled monolayer (cys-SAM) onto gold electrode (AuE) has been fabricated for
Escherichia coli (
E. coli) detection. This biosensing electrode has been characterized using scanning electron microscopy (SEM), FT-IR spectroscopy, cyclic voltammetry (CV), differential pulse voltammetry (DPV) and electrochemical impedance spectroscopy (EIS). Under the optimum conditions, this DNA biosensor can be used to detect complementary target DNA concentration in the range of 1
×
10
−
6
M to 1
×
10
−
20
M within 60
s of hybridization time at 25
°C and has been found to be stable for about four months when stored at 4
°C.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><doi>10.1016/j.tsf.2010.08.065</doi><tpages>6</tpages></addata></record> |
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| ispartof | Thin solid films, 2010-11, Vol.519 (3), p.1178-1183 |
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| language | eng |
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| source | ScienceDirect Journals (5 years ago - present) |
| subjects | Biosensors Condensed matter: structure, mechanical and thermal properties Cystine Electrochemical DNA biosensor Escherichia coli Exact sciences and technology Methylene blue Physics Self-assembled monolayer Structure and morphology thickness Surfaces and interfaces thin films and whiskers (structure and nonelectronic properties) Thin film structure and morphology |
| title | Electrochemical studies of cystine modified self-assembled monolayer for Escherichia coli detection |
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