Enhanced formation of extracellular laccase activity by the white-rot fungus Trametes multicolor
The white-rot fungus Trametes multicolor MB 49 has been identified as an excellent producer of the industrially important enzyme laccase. The formation of extracellular laccase could be considerably stimulated by the addition of Cu(II) to a simple, glycerol-based culture medium. In this study, optim...
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description | The white-rot fungus Trametes multicolor MB 49 has been identified as an excellent producer of the industrially important enzyme laccase. The formation of extracellular laccase could be considerably stimulated by the addition of Cu(II) to a simple, glycerol-based culture medium. In this study, optimal concentrations of copper were found to be 0.5-1 mM, which were added during the growth phase of the fungus. Other medium components important for laccase production are the carbon and nitrogen sources employed. When using an optimized medium containing glycerol (40 g/L), peptone from meat (15 g/L), and MgSO4 x 7H2O and stimulating enzyme formation by the addition of 1.0 mM Cu, maximal laccase activities obtained in shake-flask cultures were approx 85 U/mL. These results, however, could not be scaled up to a laboratory fermentor cultivation. Laccase production by T. multicolor decreased considerably when the fungus was grown in a stirred-tank reactor, presumably because of damage of the mycelia caused by shear stress and/or changes in the morphology of the fungus. |
doi_str_mv | 10.1385/abab:98-100:1-9:229 |
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The formation of extracellular laccase could be considerably stimulated by the addition of Cu(II) to a simple, glycerol-based culture medium. In this study, optimal concentrations of copper were found to be 0.5-1 mM, which were added during the growth phase of the fungus. Other medium components important for laccase production are the carbon and nitrogen sources employed. When using an optimized medium containing glycerol (40 g/L), peptone from meat (15 g/L), and MgSO4 x 7H2O and stimulating enzyme formation by the addition of 1.0 mM Cu, maximal laccase activities obtained in shake-flask cultures were approx 85 U/mL. These results, however, could not be scaled up to a laboratory fermentor cultivation. Laccase production by T. multicolor decreased considerably when the fungus was grown in a stirred-tank reactor, presumably because of damage of the mycelia caused by shear stress and/or changes in the morphology of the fungus.</description><identifier>ISSN: 0273-2289</identifier><identifier>EISSN: 1559-0291</identifier><identifier>EISSN: 0273-2289</identifier><identifier>DOI: 10.1385/abab:98-100:1-9:229</identifier><identifier>PMID: 12018250</identifier><identifier>CODEN: ABIBDL</identifier><language>eng</language><publisher>Heidelberg: Springer</publisher><subject>Ascomycota - drug effects ; Ascomycota - enzymology ; Ascomycota - growth & development ; Biological and medical sciences ; Biotechnology ; Carbon sources ; Copper ; Copper - pharmacology ; Culture Media ; Enzyme engineering ; Enzymes ; Fermentation ; Fundamental and applied biological sciences. Psychology ; Fungi ; Glycerol ; Kinetics ; Laccase ; Meat ; Mechanical stimuli ; Methods. Procedures. Technologies ; Microbiology ; Nitrogen sources ; Oxidoreductases - metabolism ; Peptones ; Production of selected enzymes ; Shear stress ; Trametes multicolor ; White rot ; White rot fungi</subject><ispartof>Applied biochemistry and biotechnology, 2002, Vol.98-100 (1-9), p.229-241</ispartof><rights>2002 INIST-CNRS</rights><rights>Humana Press Inc. 2002</rights><rights>Humana Press Inc. 2002.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c484t-f75c8555b6bd32b59a9a34a0bfa93dc87af60f838de5d7255d7a348c80f1182e3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>309,310,314,780,784,789,790,4024,4050,4051,23930,23931,25140,27923,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=13711558$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12018250$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>HESS, Johann</creatorcontrib><creatorcontrib>LEITNER, Christian</creatorcontrib><creatorcontrib>GALHAUP, Christiane</creatorcontrib><creatorcontrib>KULBE, Klaus D</creatorcontrib><creatorcontrib>HINTERSTOISSER, Barbara</creatorcontrib><creatorcontrib>STEINWENDER, Martin</creatorcontrib><creatorcontrib>HALTRICH, Dietmar</creatorcontrib><title>Enhanced formation of extracellular laccase activity by the white-rot fungus Trametes multicolor</title><title>Applied biochemistry and biotechnology</title><addtitle>Appl Biochem Biotechnol</addtitle><description>The white-rot fungus Trametes multicolor MB 49 has been identified as an excellent producer of the industrially important enzyme laccase. The formation of extracellular laccase could be considerably stimulated by the addition of Cu(II) to a simple, glycerol-based culture medium. In this study, optimal concentrations of copper were found to be 0.5-1 mM, which were added during the growth phase of the fungus. Other medium components important for laccase production are the carbon and nitrogen sources employed. When using an optimized medium containing glycerol (40 g/L), peptone from meat (15 g/L), and MgSO4 x 7H2O and stimulating enzyme formation by the addition of 1.0 mM Cu, maximal laccase activities obtained in shake-flask cultures were approx 85 U/mL. These results, however, could not be scaled up to a laboratory fermentor cultivation. Laccase production by T. multicolor decreased considerably when the fungus was grown in a stirred-tank reactor, presumably because of damage of the mycelia caused by shear stress and/or changes in the morphology of the fungus.</description><subject>Ascomycota - drug effects</subject><subject>Ascomycota - enzymology</subject><subject>Ascomycota - growth & development</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Carbon sources</subject><subject>Copper</subject><subject>Copper - pharmacology</subject><subject>Culture Media</subject><subject>Enzyme engineering</subject><subject>Enzymes</subject><subject>Fermentation</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fungi</subject><subject>Glycerol</subject><subject>Kinetics</subject><subject>Laccase</subject><subject>Meat</subject><subject>Mechanical stimuli</subject><subject>Methods. Procedures. Technologies</subject><subject>Microbiology</subject><subject>Nitrogen sources</subject><subject>Oxidoreductases - metabolism</subject><subject>Peptones</subject><subject>Production of selected enzymes</subject><subject>Shear stress</subject><subject>Trametes multicolor</subject><subject>White rot</subject><subject>White rot fungi</subject><issn>0273-2289</issn><issn>1559-0291</issn><issn>0273-2289</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kV9rFDEUxYModq1-AkGCUn0azZ_N5GbftqVqodCX-hzvZBJ3ysykJhntfntTulAQ9CWX5P7uuZwcQl5z9pFLUJ-ww25joOGMbXhjNkKYJ2TFlTINE4Y_JSsmtGyEAHNEXuR8wxgXoPRzcsQF4yAUW5Hv5_MOZ-d7GmKasAxxpjFQf1cSOj-Oy4iJjugcZk_RleHXUPa029Oy8_T3bii-SbHQsMw_lkyvE06--EynZSyDi2NML8mzgGP2rw71mHz7fH599rW5vPpycba9bNwa1qUJWjlQSnVt10vRKYMG5RpZF9DI3oHG0LIAEnqvei1UPWofHLDAqxUvj8mHB93bFH8uPhc7DfneAc4-LtlC_RgJXLNKvv8vqXlrtGlFBd_9Bd7EJc3VhRVtu9ZMM1CVevsvihvNeRXSFZIPkEsx5-SDvU3DhGlvObP3adrt6fbUGqj3-mCNrWnWqTcH6aWbfP84c4ivAicHALPDMaQa5ZAfOVn3KwXyD_40qAg</recordid><startdate>2002</startdate><enddate>2002</enddate><creator>HESS, Johann</creator><creator>LEITNER, Christian</creator><creator>GALHAUP, Christiane</creator><creator>KULBE, Klaus D</creator><creator>HINTERSTOISSER, Barbara</creator><creator>STEINWENDER, Martin</creator><creator>HALTRICH, Dietmar</creator><general>Springer</general><general>Springer Nature B.V</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7ST</scope><scope>7T7</scope><scope>7TM</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>RC3</scope><scope>SOI</scope><scope>7X8</scope><scope>7QO</scope><scope>M7N</scope></search><sort><creationdate>2002</creationdate><title>Enhanced formation of extracellular laccase activity by the white-rot fungus Trametes multicolor</title><author>HESS, Johann ; LEITNER, Christian ; GALHAUP, Christiane ; KULBE, Klaus D ; HINTERSTOISSER, Barbara ; STEINWENDER, Martin ; HALTRICH, Dietmar</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c484t-f75c8555b6bd32b59a9a34a0bfa93dc87af60f838de5d7255d7a348c80f1182e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Ascomycota - drug effects</topic><topic>Ascomycota - enzymology</topic><topic>Ascomycota - growth & development</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Carbon sources</topic><topic>Copper</topic><topic>Copper - pharmacology</topic><topic>Culture Media</topic><topic>Enzyme engineering</topic><topic>Enzymes</topic><topic>Fermentation</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fungi</topic><topic>Glycerol</topic><topic>Kinetics</topic><topic>Laccase</topic><topic>Meat</topic><topic>Mechanical stimuli</topic><topic>Methods. Procedures. Technologies</topic><topic>Microbiology</topic><topic>Nitrogen sources</topic><topic>Oxidoreductases - metabolism</topic><topic>Peptones</topic><topic>Production of selected enzymes</topic><topic>Shear stress</topic><topic>Trametes multicolor</topic><topic>White rot</topic><topic>White rot fungi</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>HESS, Johann</creatorcontrib><creatorcontrib>LEITNER, Christian</creatorcontrib><creatorcontrib>GALHAUP, Christiane</creatorcontrib><creatorcontrib>KULBE, Klaus D</creatorcontrib><creatorcontrib>HINTERSTOISSER, Barbara</creatorcontrib><creatorcontrib>STEINWENDER, Martin</creatorcontrib><creatorcontrib>HALTRICH, Dietmar</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Environment Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><collection>Environment Abstracts</collection><collection>MEDLINE - Academic</collection><collection>Biotechnology Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><jtitle>Applied biochemistry and biotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>HESS, Johann</au><au>LEITNER, Christian</au><au>GALHAUP, Christiane</au><au>KULBE, Klaus D</au><au>HINTERSTOISSER, Barbara</au><au>STEINWENDER, Martin</au><au>HALTRICH, Dietmar</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Enhanced formation of extracellular laccase activity by the white-rot fungus Trametes multicolor</atitle><jtitle>Applied biochemistry and biotechnology</jtitle><addtitle>Appl Biochem Biotechnol</addtitle><date>2002</date><risdate>2002</risdate><volume>98-100</volume><issue>1-9</issue><spage>229</spage><epage>241</epage><pages>229-241</pages><issn>0273-2289</issn><eissn>1559-0291</eissn><eissn>0273-2289</eissn><coden>ABIBDL</coden><abstract>The white-rot fungus Trametes multicolor MB 49 has been identified as an excellent producer of the industrially important enzyme laccase. The formation of extracellular laccase could be considerably stimulated by the addition of Cu(II) to a simple, glycerol-based culture medium. In this study, optimal concentrations of copper were found to be 0.5-1 mM, which were added during the growth phase of the fungus. Other medium components important for laccase production are the carbon and nitrogen sources employed. When using an optimized medium containing glycerol (40 g/L), peptone from meat (15 g/L), and MgSO4 x 7H2O and stimulating enzyme formation by the addition of 1.0 mM Cu, maximal laccase activities obtained in shake-flask cultures were approx 85 U/mL. These results, however, could not be scaled up to a laboratory fermentor cultivation. Laccase production by T. multicolor decreased considerably when the fungus was grown in a stirred-tank reactor, presumably because of damage of the mycelia caused by shear stress and/or changes in the morphology of the fungus.</abstract><cop>Heidelberg</cop><pub>Springer</pub><pmid>12018250</pmid><doi>10.1385/abab:98-100:1-9:229</doi><tpages>13</tpages></addata></record> |
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subjects | Ascomycota - drug effects Ascomycota - enzymology Ascomycota - growth & development Biological and medical sciences Biotechnology Carbon sources Copper Copper - pharmacology Culture Media Enzyme engineering Enzymes Fermentation Fundamental and applied biological sciences. Psychology Fungi Glycerol Kinetics Laccase Meat Mechanical stimuli Methods. Procedures. Technologies Microbiology Nitrogen sources Oxidoreductases - metabolism Peptones Production of selected enzymes Shear stress Trametes multicolor White rot White rot fungi |
title | Enhanced formation of extracellular laccase activity by the white-rot fungus Trametes multicolor |
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