Pulmonary antioxidants exert differential protective effects against urban and industrial particulate matter
This investigation focuses on the application of an in vitro assay in elucidating the role of lung lining fluid antioxidants in the protection against inhaled particles, and to compare the toxicities of different airborne particulate matter (PM), PM10, collections from South Wales, UK. PM collection...
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| Veröffentlicht in: | Journal of biosciences 2003-02, Vol.28 (1), p.101-107 |
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| description | This investigation focuses on the application of an in vitro assay in elucidating the role of lung lining fluid antioxidants in the protection against inhaled particles, and to compare the toxicities of different airborne particulate matter (PM), PM10, collections from South Wales, UK. PM collections from both urban and industrial sites caused 50% oxidative degradation of DNA in vitro at concentrations as low as 12.9 +/- 2.1 microg ml(-1) and 4.9 0.9 mg ml-1 respectively. The primary source of this bioreactivity was found to be the soluble fraction of both particle collections. The coarser PM(10-2.5) fraction also showed greater oxidative bioreactivity than the PM(2.5-0.1) in both cases. When repeated in the presence of a low molecular weight fraction of fresh pulmonary lavage fluid, as well as in artificial lung lining fluid (200 microM urate, glutathione and ascorbate), the DNA damage was significantly reduced in all cases (P < 0.05). The antioxidants exerted a greater effect on the industrial samples than on the urban samples, and on the PM(10-2.5) fractions than on the PM(2.5-0.1) fractions, supporting the previous findings that respirable PM and urban samples contain fewer free radical sources than inhalable PM and industrial samples. |
| doi_str_mv | 10.1007/BF02970139 |
| format | Article |
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PM collections from both urban and industrial sites caused 50% oxidative degradation of DNA in vitro at concentrations as low as 12.9 +/- 2.1 microg ml(-1) and 4.9 0.9 mg ml-1 respectively. The primary source of this bioreactivity was found to be the soluble fraction of both particle collections. The coarser PM(10-2.5) fraction also showed greater oxidative bioreactivity than the PM(2.5-0.1) in both cases. When repeated in the presence of a low molecular weight fraction of fresh pulmonary lavage fluid, as well as in artificial lung lining fluid (200 microM urate, glutathione and ascorbate), the DNA damage was significantly reduced in all cases (P < 0.05). The antioxidants exerted a greater effect on the industrial samples than on the urban samples, and on the PM(10-2.5) fractions than on the PM(2.5-0.1) fractions, supporting the previous findings that respirable PM and urban samples contain fewer free radical sources than inhalable PM and industrial samples.</description><identifier>ISSN: 0250-5991</identifier><identifier>EISSN: 0973-7138</identifier><identifier>DOI: 10.1007/BF02970139</identifier><identifier>PMID: 12682432</identifier><language>eng</language><publisher>India: Springer Nature B.V</publisher><subject>Air Pollutants - chemistry ; Air Pollutants - toxicity ; Airborne particulates ; Animals ; Antioxidants ; Antioxidants - physiology ; Bronchoalveolar Lavage Fluid - chemistry ; Cities ; Free radicals ; Germ-Free Life ; In Vitro Techniques ; Industry ; Lung - chemistry ; Lung - cytology ; Male ; Molecular Weight ; Oxidation-Reduction ; Particle Size ; Particulate matter ; Rats ; Rats, Sprague-Dawley ; Respiratory Insufficiency - prevention & control ; Solubility ; Toxicity Tests ; Urban areas</subject><ispartof>Journal of biosciences, 2003-02, Vol.28 (1), p.101-107</ispartof><rights>Indian Academy of Sciences 2003</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c342t-8803c30d2d07ad0ba4bb19e2d9bb9bc212eedb62c86224376cea53b14e8e7dcd3</citedby><cites>FETCH-LOGICAL-c342t-8803c30d2d07ad0ba4bb19e2d9bb9bc212eedb62c86224376cea53b14e8e7dcd3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12682432$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Greenwell, L L</creatorcontrib><creatorcontrib>Moreno, T</creatorcontrib><creatorcontrib>Richards, R J</creatorcontrib><title>Pulmonary antioxidants exert differential protective effects against urban and industrial particulate matter</title><title>Journal of biosciences</title><addtitle>J Biosci</addtitle><description>This investigation focuses on the application of an in vitro assay in elucidating the role of lung lining fluid antioxidants in the protection against inhaled particles, and to compare the toxicities of different airborne particulate matter (PM), PM10, collections from South Wales, UK. PM collections from both urban and industrial sites caused 50% oxidative degradation of DNA in vitro at concentrations as low as 12.9 +/- 2.1 microg ml(-1) and 4.9 0.9 mg ml-1 respectively. The primary source of this bioreactivity was found to be the soluble fraction of both particle collections. The coarser PM(10-2.5) fraction also showed greater oxidative bioreactivity than the PM(2.5-0.1) in both cases. When repeated in the presence of a low molecular weight fraction of fresh pulmonary lavage fluid, as well as in artificial lung lining fluid (200 microM urate, glutathione and ascorbate), the DNA damage was significantly reduced in all cases (P < 0.05). The antioxidants exerted a greater effect on the industrial samples than on the urban samples, and on the PM(10-2.5) fractions than on the PM(2.5-0.1) fractions, supporting the previous findings that respirable PM and urban samples contain fewer free radical sources than inhalable PM and industrial samples.</description><subject>Air Pollutants - chemistry</subject><subject>Air Pollutants - toxicity</subject><subject>Airborne particulates</subject><subject>Animals</subject><subject>Antioxidants</subject><subject>Antioxidants - physiology</subject><subject>Bronchoalveolar Lavage Fluid - chemistry</subject><subject>Cities</subject><subject>Free radicals</subject><subject>Germ-Free Life</subject><subject>In Vitro Techniques</subject><subject>Industry</subject><subject>Lung - chemistry</subject><subject>Lung - cytology</subject><subject>Male</subject><subject>Molecular Weight</subject><subject>Oxidation-Reduction</subject><subject>Particle Size</subject><subject>Particulate matter</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Respiratory Insufficiency - 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PM collections from both urban and industrial sites caused 50% oxidative degradation of DNA in vitro at concentrations as low as 12.9 +/- 2.1 microg ml(-1) and 4.9 0.9 mg ml-1 respectively. The primary source of this bioreactivity was found to be the soluble fraction of both particle collections. The coarser PM(10-2.5) fraction also showed greater oxidative bioreactivity than the PM(2.5-0.1) in both cases. When repeated in the presence of a low molecular weight fraction of fresh pulmonary lavage fluid, as well as in artificial lung lining fluid (200 microM urate, glutathione and ascorbate), the DNA damage was significantly reduced in all cases (P < 0.05). 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| source | MEDLINE; Indian Academy of Sciences; Springer Nature - Complete Springer Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Air Pollutants - chemistry Air Pollutants - toxicity Airborne particulates Animals Antioxidants Antioxidants - physiology Bronchoalveolar Lavage Fluid - chemistry Cities Free radicals Germ-Free Life In Vitro Techniques Industry Lung - chemistry Lung - cytology Male Molecular Weight Oxidation-Reduction Particle Size Particulate matter Rats Rats, Sprague-Dawley Respiratory Insufficiency - prevention & control Solubility Toxicity Tests Urban areas |
| title | Pulmonary antioxidants exert differential protective effects against urban and industrial particulate matter |
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