Biotype stability of Candida albicans isolates after culture storage determined by randomly amplified polymorphic DNA and phenotypical methods
Typing methods to evaluate isolates in relation to their phenotypical and molecular characteristics are essential in epidemiological studies. In this study, Candida albicans biotypes were determined before and after storage in order to verify their stability. Twenty C. albicans isolates were typed b...
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| Veröffentlicht in: | Mycoses 2010-11, Vol.53 (6), p.468-474 |
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| creator | Bacelo, Katia Leston da Costa, Karen Regina Carim Ferreira, Joseane Cristina Candido, Regina Celia |
| description | Typing methods to evaluate isolates in relation to their phenotypical and molecular characteristics are essential in epidemiological studies. In this study, Candida albicans biotypes were determined before and after storage in order to verify their stability. Twenty C. albicans isolates were typed by Randomly Amplified Polymorphic DNA (RAPD), production of phospholipase and proteinase exoenzymes (enzymotyping) and morphotyping before and after 180 days of storage in Sabouraud dextrose agar (SDA) and sterilised distilled water. Before the storage, 19 RAPD patterns, two enzymotypes and eight morphotypes were identified. The fragment patterns obtained by RAPD, on the one hand, were not significantly altered after storage. On the other hand, the majority of the isolates changed their enzymotype and morphotype after storage. RAPD typing provided the better discriminatory index (DI) among isolates (DI = 0.995) and maintained the profile identified, thereby confirming its utility in epidemiological surveys. Based on the low reproducibility observed after storage in SDA and distilled water by morphotyping (DI = 0.853) and enzymotyping (DI = 0.521), the use of these techniques is not recommended on stored isolates. |
| doi_str_mv | 10.1111/j.1439-0507.2009.01741.x |
| format | Article |
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In this study, Candida albicans biotypes were determined before and after storage in order to verify their stability. Twenty C. albicans isolates were typed by Randomly Amplified Polymorphic DNA (RAPD), production of phospholipase and proteinase exoenzymes (enzymotyping) and morphotyping before and after 180 days of storage in Sabouraud dextrose agar (SDA) and sterilised distilled water. Before the storage, 19 RAPD patterns, two enzymotypes and eight morphotypes were identified. The fragment patterns obtained by RAPD, on the one hand, were not significantly altered after storage. On the other hand, the majority of the isolates changed their enzymotype and morphotype after storage. RAPD typing provided the better discriminatory index (DI) among isolates (DI = 0.995) and maintained the profile identified, thereby confirming its utility in epidemiological surveys. Based on the low reproducibility observed after storage in SDA and distilled water by morphotyping (DI = 0.853) and enzymotyping (DI = 0.521), the use of these techniques is not recommended on stored isolates.</description><identifier>ISSN: 0933-7407</identifier><identifier>EISSN: 1439-0507</identifier><identifier>DOI: 10.1111/j.1439-0507.2009.01741.x</identifier><identifier>PMID: 19538522</identifier><language>eng</language><publisher>Oxford, UK: Oxford, UK : Blackwell Publishing Ltd</publisher><subject>Candida ; Candida albicans ; Candida albicans - classification ; Candida albicans - genetics ; Candida albicans - metabolism ; DNA, Fungal - genetics ; Fungal Proteins - metabolism ; Genomic Instability ; Genotype ; Humans ; morphotyping ; Mycological Typing Techniques ; Mycology - methods ; Peptide Hydrolases - metabolism ; Phenotype ; phospholipase ; Phospholipases - metabolism ; Preservation, Biological ; proteinase ; Random Amplified Polymorphic DNA Technique ; RAPD ; Specimen Handling - methods ; storage</subject><ispartof>Mycoses, 2010-11, Vol.53 (6), p.468-474</ispartof><rights>2009 Blackwell Verlag GmbH</rights><rights>2009 Blackwell Verlag GmbH.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4131-a9d5b2731eda5047f25455bb3f3f8cf4e4f9000a07e968da547e739c5f18a4ed3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1439-0507.2009.01741.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1439-0507.2009.01741.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19538522$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bacelo, Katia Leston</creatorcontrib><creatorcontrib>da Costa, Karen Regina Carim</creatorcontrib><creatorcontrib>Ferreira, Joseane Cristina</creatorcontrib><creatorcontrib>Candido, Regina Celia</creatorcontrib><title>Biotype stability of Candida albicans isolates after culture storage determined by randomly amplified polymorphic DNA and phenotypical methods</title><title>Mycoses</title><addtitle>Mycoses</addtitle><description>Typing methods to evaluate isolates in relation to their phenotypical and molecular characteristics are essential in epidemiological studies. In this study, Candida albicans biotypes were determined before and after storage in order to verify their stability. Twenty C. albicans isolates were typed by Randomly Amplified Polymorphic DNA (RAPD), production of phospholipase and proteinase exoenzymes (enzymotyping) and morphotyping before and after 180 days of storage in Sabouraud dextrose agar (SDA) and sterilised distilled water. Before the storage, 19 RAPD patterns, two enzymotypes and eight morphotypes were identified. The fragment patterns obtained by RAPD, on the one hand, were not significantly altered after storage. On the other hand, the majority of the isolates changed their enzymotype and morphotype after storage. RAPD typing provided the better discriminatory index (DI) among isolates (DI = 0.995) and maintained the profile identified, thereby confirming its utility in epidemiological surveys. Based on the low reproducibility observed after storage in SDA and distilled water by morphotyping (DI = 0.853) and enzymotyping (DI = 0.521), the use of these techniques is not recommended on stored isolates.</description><subject>Candida</subject><subject>Candida albicans</subject><subject>Candida albicans - classification</subject><subject>Candida albicans - genetics</subject><subject>Candida albicans - metabolism</subject><subject>DNA, Fungal - genetics</subject><subject>Fungal Proteins - metabolism</subject><subject>Genomic Instability</subject><subject>Genotype</subject><subject>Humans</subject><subject>morphotyping</subject><subject>Mycological Typing Techniques</subject><subject>Mycology - methods</subject><subject>Peptide Hydrolases - metabolism</subject><subject>Phenotype</subject><subject>phospholipase</subject><subject>Phospholipases - metabolism</subject><subject>Preservation, Biological</subject><subject>proteinase</subject><subject>Random Amplified Polymorphic DNA Technique</subject><subject>RAPD</subject><subject>Specimen Handling - methods</subject><subject>storage</subject><issn>0933-7407</issn><issn>1439-0507</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1u1DAURiMEokPhFcA7Vgn-jeMFizJAB1HKAqoRbCwnsTsenDi1E3XyEn1mHKaUJd7Yuvd8vpZPlgEEC5TWm32BKBE5ZJAXGEJRQMQpKg6PstVD43G2goKQnFPIT7JnMe5hogQun2YnSDBSMYxX2d0768d50CCOqrbOjjPwBqxV39pWAeVq26g-Ahu9U6OOQJlRB9BMbpzCEvJBXWvQ6lTtbK9bUM8gpLTv3AxUNzhrbKoO3s2dD8PONuD95RlIBBh2ul9mpwkOdHrc-TY-z54Y5aJ-cb-fZlcfP3xfb_KLr-ef1mcXeUMRQbkSLasxJ0i3ikHKDWaUsbomhpiqMVRTIyCECnItyioxlGtORMMMqhTVLTnNXh_vHYK_mXQcZWdjo51TvfZTlBVijJSIkf-SvMS4omUJE_nynpzqTrdyCLZTYZZ_PzsBb4_ArXV6_teHcpEq93JxJxd3cpEq_0iVB_nlx3o5pXx-zNs46sNDXoVfsuSEM7m9PJebn2TzebvlcnnQqyNvlJfqOtgor75hiAhEleBYcPIbjYmwLg</recordid><startdate>201011</startdate><enddate>201011</enddate><creator>Bacelo, Katia Leston</creator><creator>da Costa, Karen Regina Carim</creator><creator>Ferreira, Joseane Cristina</creator><creator>Candido, Regina Celia</creator><general>Oxford, UK : Blackwell Publishing Ltd</general><general>Blackwell Publishing Ltd</general><scope>FBQ</scope><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope><scope>7TM</scope><scope>F1W</scope><scope>H95</scope><scope>L.G</scope><scope>M7N</scope></search><sort><creationdate>201011</creationdate><title>Biotype stability of Candida albicans isolates after culture storage determined by randomly amplified polymorphic DNA and phenotypical methods</title><author>Bacelo, Katia Leston ; da Costa, Karen Regina Carim ; Ferreira, Joseane Cristina ; Candido, Regina Celia</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4131-a9d5b2731eda5047f25455bb3f3f8cf4e4f9000a07e968da547e739c5f18a4ed3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Candida</topic><topic>Candida albicans</topic><topic>Candida albicans - classification</topic><topic>Candida albicans - genetics</topic><topic>Candida albicans - metabolism</topic><topic>DNA, Fungal - genetics</topic><topic>Fungal Proteins - metabolism</topic><topic>Genomic Instability</topic><topic>Genotype</topic><topic>Humans</topic><topic>morphotyping</topic><topic>Mycological Typing Techniques</topic><topic>Mycology - methods</topic><topic>Peptide Hydrolases - metabolism</topic><topic>Phenotype</topic><topic>phospholipase</topic><topic>Phospholipases - metabolism</topic><topic>Preservation, Biological</topic><topic>proteinase</topic><topic>Random Amplified Polymorphic DNA Technique</topic><topic>RAPD</topic><topic>Specimen Handling - methods</topic><topic>storage</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bacelo, Katia Leston</creatorcontrib><creatorcontrib>da Costa, Karen Regina Carim</creatorcontrib><creatorcontrib>Ferreira, Joseane Cristina</creatorcontrib><creatorcontrib>Candido, Regina Celia</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><collection>Nucleic Acids Abstracts</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><jtitle>Mycoses</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bacelo, Katia Leston</au><au>da Costa, Karen Regina Carim</au><au>Ferreira, Joseane Cristina</au><au>Candido, Regina Celia</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Biotype stability of Candida albicans isolates after culture storage determined by randomly amplified polymorphic DNA and phenotypical methods</atitle><jtitle>Mycoses</jtitle><addtitle>Mycoses</addtitle><date>2010-11</date><risdate>2010</risdate><volume>53</volume><issue>6</issue><spage>468</spage><epage>474</epage><pages>468-474</pages><issn>0933-7407</issn><eissn>1439-0507</eissn><abstract>Typing methods to evaluate isolates in relation to their phenotypical and molecular characteristics are essential in epidemiological studies. In this study, Candida albicans biotypes were determined before and after storage in order to verify their stability. Twenty C. albicans isolates were typed by Randomly Amplified Polymorphic DNA (RAPD), production of phospholipase and proteinase exoenzymes (enzymotyping) and morphotyping before and after 180 days of storage in Sabouraud dextrose agar (SDA) and sterilised distilled water. Before the storage, 19 RAPD patterns, two enzymotypes and eight morphotypes were identified. The fragment patterns obtained by RAPD, on the one hand, were not significantly altered after storage. On the other hand, the majority of the isolates changed their enzymotype and morphotype after storage. RAPD typing provided the better discriminatory index (DI) among isolates (DI = 0.995) and maintained the profile identified, thereby confirming its utility in epidemiological surveys. Based on the low reproducibility observed after storage in SDA and distilled water by morphotyping (DI = 0.853) and enzymotyping (DI = 0.521), the use of these techniques is not recommended on stored isolates.</abstract><cop>Oxford, UK</cop><pub>Oxford, UK : Blackwell Publishing Ltd</pub><pmid>19538522</pmid><doi>10.1111/j.1439-0507.2009.01741.x</doi><tpages>7</tpages></addata></record> |
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| subjects | Candida Candida albicans Candida albicans - classification Candida albicans - genetics Candida albicans - metabolism DNA, Fungal - genetics Fungal Proteins - metabolism Genomic Instability Genotype Humans morphotyping Mycological Typing Techniques Mycology - methods Peptide Hydrolases - metabolism Phenotype phospholipase Phospholipases - metabolism Preservation, Biological proteinase Random Amplified Polymorphic DNA Technique RAPD Specimen Handling - methods storage |
| title | Biotype stability of Candida albicans isolates after culture storage determined by randomly amplified polymorphic DNA and phenotypical methods |
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