Chitooligosaccharides enzymatic production by Metarhizium anisopliae
The products of chitosan hydrolysis are chitooligosaccharides and are used mainly for medical applications due to their specific biological activities. The objective of this study was to detect and identify the products of enzymatic hydrolysis of chitosan (dimers to hexamers) using a crude extract o...
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| Veröffentlicht in: | Bioprocess and biosystems engineering 2010-09, Vol.33 (7), p.893-899 |
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| creator | de Assis, Cristiane Fernandes Araújo, Nathália Kelly Pagnoncelli, Maria Giovana Binder da Silva Pedrini, Márcia Regina de Macedo, Gorete Ribeiro dos Santos, Everaldo Silvino |
| description | The products of chitosan hydrolysis are chitooligosaccharides and are used mainly for medical applications due to their specific biological activities. The objective of this study was to detect and identify the products of enzymatic hydrolysis of chitosan (dimers to hexamers) using a crude extract of chitosanolytic enzymes produced by the fungus Metarhizium anisopliae. These fungus was able to produce, during 48 h cultivation in a medium containing chitosan, chitooligosaccharides ranging from dimers, trimers, tetramers and pentamers at concentrations 0.2, 0.19, 0.06, 0.04 mg/mL, respectively, and the enzymatic activity was 2.5 U/L. Using the crude enzyme extract for chitosan hydrolysis, we detected the presence of dimers to hexamers at hydrolysis times of 10, 20, 30, 40, 50 and 60 min of enzymatic reaction, but the yields were higher at 10 min (54%). The hexamers was obtained only with 30 min of reaction with concentration of 0.004 mg/mL. |
| doi_str_mv | 10.1007/s00449-010-0412-z |
| format | Article |
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The objective of this study was to detect and identify the products of enzymatic hydrolysis of chitosan (dimers to hexamers) using a crude extract of chitosanolytic enzymes produced by the fungus Metarhizium anisopliae. These fungus was able to produce, during 48 h cultivation in a medium containing chitosan, chitooligosaccharides ranging from dimers, trimers, tetramers and pentamers at concentrations 0.2, 0.19, 0.06, 0.04 mg/mL, respectively, and the enzymatic activity was 2.5 U/L. Using the crude enzyme extract for chitosan hydrolysis, we detected the presence of dimers to hexamers at hydrolysis times of 10, 20, 30, 40, 50 and 60 min of enzymatic reaction, but the yields were higher at 10 min (54%). The hexamers was obtained only with 30 min of reaction with concentration of 0.004 mg/mL.</description><identifier>ISSN: 1615-7591</identifier><identifier>EISSN: 1615-7605</identifier><identifier>DOI: 10.1007/s00449-010-0412-z</identifier><identifier>PMID: 20165886</identifier><language>eng</language><publisher>Berlin/Heidelberg: Berlin/Heidelberg : Springer-Verlag</publisher><subject>Biotechnology ; Carbohydrates ; Chemical reactions ; Chemistry ; Chemistry and Materials Science ; Chitooligosaccharides ; chitosan ; Chitosan - chemistry ; Chitosan - metabolism ; Environmental Engineering/Biotechnology ; Enzymatic activity ; Enzymes ; Food Science ; Fungi ; Hydrolysis ; Hydrolysis of chitosan ; Industrial and Production Engineering ; Industrial Chemistry/Chemical Engineering ; Metarhizium - enzymology ; Metarhizium anisopliae ; Oligosaccharides - chemistry ; Oligosaccharides - metabolism ; Original Paper ; Palavras Chaves: Metarhizium anisopliae</subject><ispartof>Bioprocess and biosystems engineering, 2010-09, Vol.33 (7), p.893-899</ispartof><rights>Springer-Verlag 2010</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c426t-fa8a3f4318e6b812d58b2378f911965e63691ee6d760f1320c57e1432d8df5f13</citedby><cites>FETCH-LOGICAL-c426t-fa8a3f4318e6b812d58b2378f911965e63691ee6d760f1320c57e1432d8df5f13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00449-010-0412-z$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00449-010-0412-z$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20165886$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>de Assis, Cristiane Fernandes</creatorcontrib><creatorcontrib>Araújo, Nathália Kelly</creatorcontrib><creatorcontrib>Pagnoncelli, Maria Giovana Binder</creatorcontrib><creatorcontrib>da Silva Pedrini, Márcia Regina</creatorcontrib><creatorcontrib>de Macedo, Gorete Ribeiro</creatorcontrib><creatorcontrib>dos Santos, Everaldo Silvino</creatorcontrib><title>Chitooligosaccharides enzymatic production by Metarhizium anisopliae</title><title>Bioprocess and biosystems engineering</title><addtitle>Bioprocess Biosyst Eng</addtitle><addtitle>Bioprocess Biosyst Eng</addtitle><description>The products of chitosan hydrolysis are chitooligosaccharides and are used mainly for medical applications due to their specific biological activities. The objective of this study was to detect and identify the products of enzymatic hydrolysis of chitosan (dimers to hexamers) using a crude extract of chitosanolytic enzymes produced by the fungus Metarhizium anisopliae. These fungus was able to produce, during 48 h cultivation in a medium containing chitosan, chitooligosaccharides ranging from dimers, trimers, tetramers and pentamers at concentrations 0.2, 0.19, 0.06, 0.04 mg/mL, respectively, and the enzymatic activity was 2.5 U/L. Using the crude enzyme extract for chitosan hydrolysis, we detected the presence of dimers to hexamers at hydrolysis times of 10, 20, 30, 40, 50 and 60 min of enzymatic reaction, but the yields were higher at 10 min (54%). The hexamers was obtained only with 30 min of reaction with concentration of 0.004 mg/mL.</description><subject>Biotechnology</subject><subject>Carbohydrates</subject><subject>Chemical reactions</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Chitooligosaccharides</subject><subject>chitosan</subject><subject>Chitosan - chemistry</subject><subject>Chitosan - metabolism</subject><subject>Environmental Engineering/Biotechnology</subject><subject>Enzymatic activity</subject><subject>Enzymes</subject><subject>Food Science</subject><subject>Fungi</subject><subject>Hydrolysis</subject><subject>Hydrolysis of chitosan</subject><subject>Industrial and Production Engineering</subject><subject>Industrial Chemistry/Chemical Engineering</subject><subject>Metarhizium - enzymology</subject><subject>Metarhizium anisopliae</subject><subject>Oligosaccharides - chemistry</subject><subject>Oligosaccharides - metabolism</subject><subject>Original Paper</subject><subject>Palavras Chaves: Metarhizium anisopliae</subject><issn>1615-7591</issn><issn>1615-7605</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqFkU1P3DAQhi1UBBT4Ab2UqJeeQmf8FftYbYFWouqhcLa8ibNrlMSLnRx2f329Ch8SB3ryyH7ed8bzEvIJ4RIBqm8JgHNdAkIJHGm5OyAnKFGUlQTx4bkWGo_Jx5QeAFAoCkfkmAJKoZQ8IT8Waz-G0PlVSLau1zb6xqXCDbttb0dfF5sYmqkefRiK5bb47UYb137np76wg09h03nrzshha7vkzp_OU3J_fXW3-Fne_rn5tfh-W9acyrFsrbKs5QyVk0uFtBFqSVmlWo2opXCSSY3OySaP3yKjUIvKIWe0UU0r8s0p-Tr75qEeJ5dG0_tUu66zgwtTMgqFYIIK_V-y4kpzKWBPfnlDPoQpDvkbGZKcac54hnCG6hhSiq41m-h7G7cGweyjMHMUJkdh9lGYXdZ8fjKelr1rXhTPu88AnYGUn4aVi6-d33O9mEWtDcauok_m_m-2ZICqUkJr9g-wCpyv</recordid><startdate>20100901</startdate><enddate>20100901</enddate><creator>de Assis, Cristiane Fernandes</creator><creator>Araújo, Nathália Kelly</creator><creator>Pagnoncelli, Maria Giovana Binder</creator><creator>da Silva Pedrini, Márcia Regina</creator><creator>de Macedo, Gorete Ribeiro</creator><creator>dos Santos, Everaldo Silvino</creator><general>Berlin/Heidelberg : Springer-Verlag</general><general>Springer-Verlag</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T7</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7X8</scope><scope>7QO</scope></search><sort><creationdate>20100901</creationdate><title>Chitooligosaccharides enzymatic production by Metarhizium anisopliae</title><author>de Assis, Cristiane Fernandes ; Araújo, Nathália Kelly ; Pagnoncelli, Maria Giovana Binder ; da Silva Pedrini, Márcia Regina ; de Macedo, Gorete Ribeiro ; dos Santos, Everaldo Silvino</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c426t-fa8a3f4318e6b812d58b2378f911965e63691ee6d760f1320c57e1432d8df5f13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Biotechnology</topic><topic>Carbohydrates</topic><topic>Chemical reactions</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Chitooligosaccharides</topic><topic>chitosan</topic><topic>Chitosan - chemistry</topic><topic>Chitosan - metabolism</topic><topic>Environmental Engineering/Biotechnology</topic><topic>Enzymatic activity</topic><topic>Enzymes</topic><topic>Food Science</topic><topic>Fungi</topic><topic>Hydrolysis</topic><topic>Hydrolysis of chitosan</topic><topic>Industrial and Production Engineering</topic><topic>Industrial Chemistry/Chemical Engineering</topic><topic>Metarhizium - enzymology</topic><topic>Metarhizium anisopliae</topic><topic>Oligosaccharides - chemistry</topic><topic>Oligosaccharides - metabolism</topic><topic>Original Paper</topic><topic>Palavras Chaves: Metarhizium anisopliae</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>de Assis, Cristiane Fernandes</creatorcontrib><creatorcontrib>Araújo, Nathália Kelly</creatorcontrib><creatorcontrib>Pagnoncelli, Maria Giovana Binder</creatorcontrib><creatorcontrib>da Silva Pedrini, Márcia Regina</creatorcontrib><creatorcontrib>de Macedo, Gorete Ribeiro</creatorcontrib><creatorcontrib>dos Santos, Everaldo Silvino</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>Biotechnology Research Abstracts</collection><jtitle>Bioprocess and biosystems engineering</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>de Assis, Cristiane Fernandes</au><au>Araújo, Nathália Kelly</au><au>Pagnoncelli, Maria Giovana Binder</au><au>da Silva Pedrini, Márcia Regina</au><au>de Macedo, Gorete Ribeiro</au><au>dos Santos, Everaldo Silvino</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Chitooligosaccharides enzymatic production by Metarhizium anisopliae</atitle><jtitle>Bioprocess and biosystems engineering</jtitle><stitle>Bioprocess Biosyst Eng</stitle><addtitle>Bioprocess Biosyst Eng</addtitle><date>2010-09-01</date><risdate>2010</risdate><volume>33</volume><issue>7</issue><spage>893</spage><epage>899</epage><pages>893-899</pages><issn>1615-7591</issn><eissn>1615-7605</eissn><abstract>The products of chitosan hydrolysis are chitooligosaccharides and are used mainly for medical applications due to their specific biological activities. The objective of this study was to detect and identify the products of enzymatic hydrolysis of chitosan (dimers to hexamers) using a crude extract of chitosanolytic enzymes produced by the fungus Metarhizium anisopliae. These fungus was able to produce, during 48 h cultivation in a medium containing chitosan, chitooligosaccharides ranging from dimers, trimers, tetramers and pentamers at concentrations 0.2, 0.19, 0.06, 0.04 mg/mL, respectively, and the enzymatic activity was 2.5 U/L. Using the crude enzyme extract for chitosan hydrolysis, we detected the presence of dimers to hexamers at hydrolysis times of 10, 20, 30, 40, 50 and 60 min of enzymatic reaction, but the yields were higher at 10 min (54%). The hexamers was obtained only with 30 min of reaction with concentration of 0.004 mg/mL.</abstract><cop>Berlin/Heidelberg</cop><pub>Berlin/Heidelberg : Springer-Verlag</pub><pmid>20165886</pmid><doi>10.1007/s00449-010-0412-z</doi><tpages>7</tpages></addata></record> |
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| subjects | Biotechnology Carbohydrates Chemical reactions Chemistry Chemistry and Materials Science Chitooligosaccharides chitosan Chitosan - chemistry Chitosan - metabolism Environmental Engineering/Biotechnology Enzymatic activity Enzymes Food Science Fungi Hydrolysis Hydrolysis of chitosan Industrial and Production Engineering Industrial Chemistry/Chemical Engineering Metarhizium - enzymology Metarhizium anisopliae Oligosaccharides - chemistry Oligosaccharides - metabolism Original Paper Palavras Chaves: Metarhizium anisopliae |
| title | Chitooligosaccharides enzymatic production by Metarhizium anisopliae |
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