Aged Beagle dogs have decreased neutrophil phagocytosis and neutrophil-related gene expression compared to younger dogs
Information on aging and the innate immune response in dogs is needed in order to understand the impact of age on the innate immune system and to design diets that might improve age-associated changes in innate immunity. The purpose of this study was to investigate the effects of age on phagocytosis...
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| Veröffentlicht in: | Veterinary immunology and immunopathology 2010-09, Vol.137 (1), p.130-135 |
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| creator | Hall, Jean A. Chinn, Rachel M. Vorachek, William R. Gorman, M. Elena Jewell, Dennis E. |
| description | Information on aging and the innate immune response in dogs is needed in order to understand the impact of age on the innate immune system and to design diets that might improve age-associated changes in innate immunity. The purpose of this study was to investigate the effects of age on phagocytosis and antibacterial activity of peripheral blood neutrophils, and on expression levels of selected mRNA specific for neutrophil migration or killing functions. Three groups of Beagle dogs were evaluated in a cross-sectional study design: puppies less than 1 year of age (
n
=
15), adults 1–7 years of age (
n
=
37) and senior adults over 8 years of age (
n
=
25). Neutrophil phagocytosis and killing of
Lactococcus lactis were assessed, and the relative abundance of mRNAs for myeloperoxidase (MPO),
l-selectin, interleukin-8 receptor (IL-8R) and interleukin-1β-converting enzyme (ICE) was determined. Results showed that in Beagle dogs there is a significant effect of age on percent bacterial killing by neutrophils. Average decline in phagocytic ability at 4 and 10 years of age (compared to 1 year of age) was 25% and 39%, respectively. In addition, younger dogs have significantly higher levels of mRNA for IL-8R,
l-selectin and ICE. These results suggest that older dogs have depressed innate immune responses compared with younger dogs, which may contribute to increased morbidity and mortality with aging. |
| doi_str_mv | 10.1016/j.vetimm.2010.05.002 |
| format | Article |
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n
=
15), adults 1–7 years of age (
n
=
37) and senior adults over 8 years of age (
n
=
25). Neutrophil phagocytosis and killing of
Lactococcus lactis were assessed, and the relative abundance of mRNAs for myeloperoxidase (MPO),
l-selectin, interleukin-8 receptor (IL-8R) and interleukin-1β-converting enzyme (ICE) was determined. Results showed that in Beagle dogs there is a significant effect of age on percent bacterial killing by neutrophils. Average decline in phagocytic ability at 4 and 10 years of age (compared to 1 year of age) was 25% and 39%, respectively. In addition, younger dogs have significantly higher levels of mRNA for IL-8R,
l-selectin and ICE. These results suggest that older dogs have depressed innate immune responses compared with younger dogs, which may contribute to increased morbidity and mortality with aging.</description><identifier>ISSN: 0165-2427</identifier><identifier>EISSN: 1873-2534</identifier><identifier>DOI: 10.1016/j.vetimm.2010.05.002</identifier><identifier>PMID: 20605222</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>adult animals ; Age Factors ; Aging ; Aging - immunology ; Animals ; bacterial infections ; Beagle ; caspase-1 ; Cell Movement ; disease resistance ; Dog ; Dogs ; Female ; gene expression ; ICE ; IL-8R ; immune response ; immunity ; interleukins ; L-selectin ; Lactococcus lactis ; Lactococcus lactis - immunology ; Male ; messenger RNA ; myeloperoxidase ; Myeloperoxidase gene expression by PCR ; Neutrophil function ; neutrophils ; Neutrophils - immunology ; Neutrophils - metabolism ; old animals ; Phagocytosis ; RNA, Messenger - analysis ; senescence ; young animals</subject><ispartof>Veterinary immunology and immunopathology, 2010-09, Vol.137 (1), p.130-135</ispartof><rights>2010</rights><rights>Published by Elsevier B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c417t-72774ebc3abc458fb0b60df4487e299b0cbfcea5fa83620b325fc0f4f27d9a483</citedby><cites>FETCH-LOGICAL-c417t-72774ebc3abc458fb0b60df4487e299b0cbfcea5fa83620b325fc0f4f27d9a483</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.vetimm.2010.05.002$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>315,781,785,3551,27929,27930,46000</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20605222$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hall, Jean A.</creatorcontrib><creatorcontrib>Chinn, Rachel M.</creatorcontrib><creatorcontrib>Vorachek, William R.</creatorcontrib><creatorcontrib>Gorman, M. Elena</creatorcontrib><creatorcontrib>Jewell, Dennis E.</creatorcontrib><title>Aged Beagle dogs have decreased neutrophil phagocytosis and neutrophil-related gene expression compared to younger dogs</title><title>Veterinary immunology and immunopathology</title><addtitle>Vet Immunol Immunopathol</addtitle><description>Information on aging and the innate immune response in dogs is needed in order to understand the impact of age on the innate immune system and to design diets that might improve age-associated changes in innate immunity. The purpose of this study was to investigate the effects of age on phagocytosis and antibacterial activity of peripheral blood neutrophils, and on expression levels of selected mRNA specific for neutrophil migration or killing functions. Three groups of Beagle dogs were evaluated in a cross-sectional study design: puppies less than 1 year of age (
n
=
15), adults 1–7 years of age (
n
=
37) and senior adults over 8 years of age (
n
=
25). Neutrophil phagocytosis and killing of
Lactococcus lactis were assessed, and the relative abundance of mRNAs for myeloperoxidase (MPO),
l-selectin, interleukin-8 receptor (IL-8R) and interleukin-1β-converting enzyme (ICE) was determined. Results showed that in Beagle dogs there is a significant effect of age on percent bacterial killing by neutrophils. Average decline in phagocytic ability at 4 and 10 years of age (compared to 1 year of age) was 25% and 39%, respectively. In addition, younger dogs have significantly higher levels of mRNA for IL-8R,
l-selectin and ICE. These results suggest that older dogs have depressed innate immune responses compared with younger dogs, which may contribute to increased morbidity and mortality with aging.</description><subject>adult animals</subject><subject>Age Factors</subject><subject>Aging</subject><subject>Aging - immunology</subject><subject>Animals</subject><subject>bacterial infections</subject><subject>Beagle</subject><subject>caspase-1</subject><subject>Cell Movement</subject><subject>disease resistance</subject><subject>Dog</subject><subject>Dogs</subject><subject>Female</subject><subject>gene expression</subject><subject>ICE</subject><subject>IL-8R</subject><subject>immune response</subject><subject>immunity</subject><subject>interleukins</subject><subject>L-selectin</subject><subject>Lactococcus lactis</subject><subject>Lactococcus lactis - immunology</subject><subject>Male</subject><subject>messenger RNA</subject><subject>myeloperoxidase</subject><subject>Myeloperoxidase gene expression by PCR</subject><subject>Neutrophil function</subject><subject>neutrophils</subject><subject>Neutrophils - immunology</subject><subject>Neutrophils - metabolism</subject><subject>old animals</subject><subject>Phagocytosis</subject><subject>RNA, Messenger - analysis</subject><subject>senescence</subject><subject>young animals</subject><issn>0165-2427</issn><issn>1873-2534</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1v1DAQhi0EokvhHyDIjVOWsWPHzgWpVHxJlThAz5bjjLNeJXGwk4X997ikIE5wsuX3mRlrHkKeU9hToPXr4_6Eix_HPYP8BGIPwB6QHVWyKpmo-EOyy5goGWfygjxJ6QgAolHqMblgUINgjO3I96seu-Itmn7Aogt9Kg7mlG9oI5qUownXJYb54IdiPpg-2PMSkk-Fmf7OyoiDWTLe44QF_pgjpuTDVNgwzibmYAnFOaxTj_HXmKfkkTNDwmf35yW5ff_u6_XH8ubzh0_XVzel5VQupWRScmxtZVrLhXIttDV0jnMlkTVNC7Z1Fo1wRlU1g7Ziwllw3DHZNYar6pK82vrOMXxbMS169MniMJgJw5q0oiLvikP1X1Jy1QjGpcgk30gbQ0oRnZ6jH008awr6zo0-6s2NvnOjQejsJpe9uB-wtiN2f4p-y8jAyw1wJmjTR5_07ZfcoQKqFDBaZ-LNRmBe2clj1Ml6nCx2PqJddBf8v__wE5u4ra4</recordid><startdate>20100915</startdate><enddate>20100915</enddate><creator>Hall, Jean A.</creator><creator>Chinn, Rachel M.</creator><creator>Vorachek, William R.</creator><creator>Gorman, M. Elena</creator><creator>Jewell, Dennis E.</creator><general>Elsevier B.V</general><general>Amsterdam: Elsevier</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QL</scope><scope>7T5</scope><scope>C1K</scope><scope>H94</scope></search><sort><creationdate>20100915</creationdate><title>Aged Beagle dogs have decreased neutrophil phagocytosis and neutrophil-related gene expression compared to younger dogs</title><author>Hall, Jean A. ; Chinn, Rachel M. ; Vorachek, William R. ; Gorman, M. Elena ; Jewell, Dennis E.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c417t-72774ebc3abc458fb0b60df4487e299b0cbfcea5fa83620b325fc0f4f27d9a483</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>adult animals</topic><topic>Age Factors</topic><topic>Aging</topic><topic>Aging - immunology</topic><topic>Animals</topic><topic>bacterial infections</topic><topic>Beagle</topic><topic>caspase-1</topic><topic>Cell Movement</topic><topic>disease resistance</topic><topic>Dog</topic><topic>Dogs</topic><topic>Female</topic><topic>gene expression</topic><topic>ICE</topic><topic>IL-8R</topic><topic>immune response</topic><topic>immunity</topic><topic>interleukins</topic><topic>L-selectin</topic><topic>Lactococcus lactis</topic><topic>Lactococcus lactis - immunology</topic><topic>Male</topic><topic>messenger RNA</topic><topic>myeloperoxidase</topic><topic>Myeloperoxidase gene expression by PCR</topic><topic>Neutrophil function</topic><topic>neutrophils</topic><topic>Neutrophils - immunology</topic><topic>Neutrophils - metabolism</topic><topic>old animals</topic><topic>Phagocytosis</topic><topic>RNA, Messenger - analysis</topic><topic>senescence</topic><topic>young animals</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hall, Jean A.</creatorcontrib><creatorcontrib>Chinn, Rachel M.</creatorcontrib><creatorcontrib>Vorachek, William R.</creatorcontrib><creatorcontrib>Gorman, M. Elena</creatorcontrib><creatorcontrib>Jewell, Dennis E.</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Immunology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>AIDS and Cancer Research Abstracts</collection><jtitle>Veterinary immunology and immunopathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hall, Jean A.</au><au>Chinn, Rachel M.</au><au>Vorachek, William R.</au><au>Gorman, M. Elena</au><au>Jewell, Dennis E.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Aged Beagle dogs have decreased neutrophil phagocytosis and neutrophil-related gene expression compared to younger dogs</atitle><jtitle>Veterinary immunology and immunopathology</jtitle><addtitle>Vet Immunol Immunopathol</addtitle><date>2010-09-15</date><risdate>2010</risdate><volume>137</volume><issue>1</issue><spage>130</spage><epage>135</epage><pages>130-135</pages><issn>0165-2427</issn><eissn>1873-2534</eissn><abstract>Information on aging and the innate immune response in dogs is needed in order to understand the impact of age on the innate immune system and to design diets that might improve age-associated changes in innate immunity. The purpose of this study was to investigate the effects of age on phagocytosis and antibacterial activity of peripheral blood neutrophils, and on expression levels of selected mRNA specific for neutrophil migration or killing functions. Three groups of Beagle dogs were evaluated in a cross-sectional study design: puppies less than 1 year of age (
n
=
15), adults 1–7 years of age (
n
=
37) and senior adults over 8 years of age (
n
=
25). Neutrophil phagocytosis and killing of
Lactococcus lactis were assessed, and the relative abundance of mRNAs for myeloperoxidase (MPO),
l-selectin, interleukin-8 receptor (IL-8R) and interleukin-1β-converting enzyme (ICE) was determined. Results showed that in Beagle dogs there is a significant effect of age on percent bacterial killing by neutrophils. Average decline in phagocytic ability at 4 and 10 years of age (compared to 1 year of age) was 25% and 39%, respectively. In addition, younger dogs have significantly higher levels of mRNA for IL-8R,
l-selectin and ICE. These results suggest that older dogs have depressed innate immune responses compared with younger dogs, which may contribute to increased morbidity and mortality with aging.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>20605222</pmid><doi>10.1016/j.vetimm.2010.05.002</doi><tpages>6</tpages></addata></record> |
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| ispartof | Veterinary immunology and immunopathology, 2010-09, Vol.137 (1), p.130-135 |
| issn | 0165-2427 1873-2534 |
| language | eng |
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| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | adult animals Age Factors Aging Aging - immunology Animals bacterial infections Beagle caspase-1 Cell Movement disease resistance Dog Dogs Female gene expression ICE IL-8R immune response immunity interleukins L-selectin Lactococcus lactis Lactococcus lactis - immunology Male messenger RNA myeloperoxidase Myeloperoxidase gene expression by PCR Neutrophil function neutrophils Neutrophils - immunology Neutrophils - metabolism old animals Phagocytosis RNA, Messenger - analysis senescence young animals |
| title | Aged Beagle dogs have decreased neutrophil phagocytosis and neutrophil-related gene expression compared to younger dogs |
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