Preservation of aerial conidia and biomasses from entomopathogenic fungi Beauveria brongniartii and Metarhizium anisopliae during lyophilization
In this study, we assessed the stability provided by different formulations to aerial conidia or biomasses (conidia, blastospores, and mycelia) of Beauveria brongniartii and Metarhizium anisopliae subjected to lyophilization. First, the impact of the freezing and drying processes on spore survival w...
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| Veröffentlicht in: | Journal of invertebrate pathology 2010-09, Vol.105 (1), p.16-23 |
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| creator | Toegel, Stefan Salar-Behzadi, Sharareh Horaczek-Clausen, Andrea Viernstein, Helmut |
| description | In this study, we assessed the stability provided by different formulations to aerial conidia or biomasses (conidia, blastospores, and mycelia) of
Beauveria brongniartii and
Metarhizium anisopliae subjected to lyophilization. First, the impact of the freezing and drying processes on spore survival was evaluated. Whereas unprotected
B. brongniartii spores showed high cryosensitivity, those of
M. anisopliae were markedly harmed by the drying process. Then, the protective efficiency of 14 excipients was systematically evaluated and optimized regarding required concentrations. Fructose, glucose, and saccharose significantly enhanced viabilities for
B. brongniartii and
M. anisopliae spores following lyophilization, especially as a result of their cryoprotective effects. In addition, the effect of various bulking agents on spore survival was studied and dextran 4 was selected to enhance the physical properties of the lyophilized products. The combination of fructose and dextran 4 was further applied to prepare lyophilized biomasses of both fungi. In comparison to freshly harvested biomasses, the lyophilized products showed similar growth rates and a comparable production of virulent secondary metabolites such as destruxin A, destruxin B, or oosporein, suggesting their applicability as biological control agents. |
| doi_str_mv | 10.1016/j.jip.2010.05.004 |
| format | Article |
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Beauveria brongniartii and
Metarhizium anisopliae subjected to lyophilization. First, the impact of the freezing and drying processes on spore survival was evaluated. Whereas unprotected
B. brongniartii spores showed high cryosensitivity, those of
M. anisopliae were markedly harmed by the drying process. Then, the protective efficiency of 14 excipients was systematically evaluated and optimized regarding required concentrations. Fructose, glucose, and saccharose significantly enhanced viabilities for
B. brongniartii and
M. anisopliae spores following lyophilization, especially as a result of their cryoprotective effects. In addition, the effect of various bulking agents on spore survival was studied and dextran 4 was selected to enhance the physical properties of the lyophilized products. The combination of fructose and dextran 4 was further applied to prepare lyophilized biomasses of both fungi. In comparison to freshly harvested biomasses, the lyophilized products showed similar growth rates and a comparable production of virulent secondary metabolites such as destruxin A, destruxin B, or oosporein, suggesting their applicability as biological control agents.</description><identifier>ISSN: 0022-2011</identifier><identifier>EISSN: 1096-0805</identifier><identifier>DOI: 10.1016/j.jip.2010.05.004</identifier><identifier>PMID: 20457163</identifier><identifier>CODEN: JIVPAZ</identifier><language>eng</language><publisher>Amsterdam: Elsevier Inc</publisher><subject>Animals ; Beauveria - drug effects ; Beauveria - physiology ; Beauveria brongniartii ; Biological and medical sciences ; Biological control ; Biomass ; Control ; Cryopreservation - methods ; Cryoprotection ; Cryoprotective Agents - pharmacology ; Dextrans - pharmacology ; Formulation ; Freeze Drying - methods ; Fructose - pharmacology ; Fundamental and applied biological sciences. Psychology ; Imbibitional damage ; Lyoprotection ; Metarhizium - drug effects ; Metarhizium - physiology ; Metarhizium anisopliae ; Pest Control, Biological - methods ; Phytopathology. Animal pests. Plant and forest protection ; Protozoa. Invertebrates ; Spores, Fungal - drug effects ; Spores, Fungal - physiology</subject><ispartof>Journal of invertebrate pathology, 2010-09, Vol.105 (1), p.16-23</ispartof><rights>2010 Elsevier Inc.</rights><rights>2015 INIST-CNRS</rights><rights>Copyright 2010 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c414t-4afed88fc5ef5754d3ac00c36cc62166ddbef88c596a2fe737aac04baf111a843</citedby><cites>FETCH-LOGICAL-c414t-4afed88fc5ef5754d3ac00c36cc62166ddbef88c596a2fe737aac04baf111a843</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0022201110001096$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27903,27904,65309</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=23233329$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20457163$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Toegel, Stefan</creatorcontrib><creatorcontrib>Salar-Behzadi, Sharareh</creatorcontrib><creatorcontrib>Horaczek-Clausen, Andrea</creatorcontrib><creatorcontrib>Viernstein, Helmut</creatorcontrib><title>Preservation of aerial conidia and biomasses from entomopathogenic fungi Beauveria brongniartii and Metarhizium anisopliae during lyophilization</title><title>Journal of invertebrate pathology</title><addtitle>J Invertebr Pathol</addtitle><description>In this study, we assessed the stability provided by different formulations to aerial conidia or biomasses (conidia, blastospores, and mycelia) of
Beauveria brongniartii and
Metarhizium anisopliae subjected to lyophilization. First, the impact of the freezing and drying processes on spore survival was evaluated. Whereas unprotected
B. brongniartii spores showed high cryosensitivity, those of
M. anisopliae were markedly harmed by the drying process. Then, the protective efficiency of 14 excipients was systematically evaluated and optimized regarding required concentrations. Fructose, glucose, and saccharose significantly enhanced viabilities for
B. brongniartii and
M. anisopliae spores following lyophilization, especially as a result of their cryoprotective effects. In addition, the effect of various bulking agents on spore survival was studied and dextran 4 was selected to enhance the physical properties of the lyophilized products. The combination of fructose and dextran 4 was further applied to prepare lyophilized biomasses of both fungi. In comparison to freshly harvested biomasses, the lyophilized products showed similar growth rates and a comparable production of virulent secondary metabolites such as destruxin A, destruxin B, or oosporein, suggesting their applicability as biological control agents.</description><subject>Animals</subject><subject>Beauveria - drug effects</subject><subject>Beauveria - physiology</subject><subject>Beauveria brongniartii</subject><subject>Biological and medical sciences</subject><subject>Biological control</subject><subject>Biomass</subject><subject>Control</subject><subject>Cryopreservation - methods</subject><subject>Cryoprotection</subject><subject>Cryoprotective Agents - pharmacology</subject><subject>Dextrans - pharmacology</subject><subject>Formulation</subject><subject>Freeze Drying - methods</subject><subject>Fructose - pharmacology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Imbibitional damage</subject><subject>Lyoprotection</subject><subject>Metarhizium - drug effects</subject><subject>Metarhizium - physiology</subject><subject>Metarhizium anisopliae</subject><subject>Pest Control, Biological - methods</subject><subject>Phytopathology. Animal pests. Plant and forest protection</subject><subject>Protozoa. Invertebrates</subject><subject>Spores, Fungal - drug effects</subject><subject>Spores, Fungal - physiology</subject><issn>0022-2011</issn><issn>1096-0805</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkcuO0zAUhi0EYsrAA7BB3iBWKXYc5yJWzIibNAgWsLZOneP2VIkd7KTSzFPwyLjTAjtY2Uf-_l9H_hh7LsVaClm_3q_3NK1LkWeh10JUD9hKiq4uRCv0Q7YSoiyL_Cwv2JOU9iLfdN09ZhelqHQja7ViP79GTBgPMFPwPDgOGAkGboOnnoCD7_mGwggpYeIuhpGjn8MYJph3YYueLHeL3xK_QlgOxzDfxOC3niDORPcFn3GGuKM7WsY8UwrTQIC8XyL5LR9uw7Sjge7ud3jKHjkYEj47n5fs-_t3364_FjdfPny6fntT2EpWc1GBw75tndXodKOrXoEVwqra2rqUdd33G3Rta3VXQ-mwUQ1koNqAk1JCW6lL9urUO8XwY8E0m5GSxWEAj2FJppVaK9Up8V-yqdquUZ08dsoTaWNIKaIzU6QR4q2RwhyNmb3JxszRmBHaZGM58-LcvmxG7P8kfivKwMszAMnC4CJ4S-kvp0qlVNll7s2Jw_xrB8JokiX0FnuKaGfTB_rHGr8AJpy4LA</recordid><startdate>20100901</startdate><enddate>20100901</enddate><creator>Toegel, Stefan</creator><creator>Salar-Behzadi, Sharareh</creator><creator>Horaczek-Clausen, Andrea</creator><creator>Viernstein, Helmut</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>M7N</scope></search><sort><creationdate>20100901</creationdate><title>Preservation of aerial conidia and biomasses from entomopathogenic fungi Beauveria brongniartii and Metarhizium anisopliae during lyophilization</title><author>Toegel, Stefan ; Salar-Behzadi, Sharareh ; Horaczek-Clausen, Andrea ; Viernstein, Helmut</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c414t-4afed88fc5ef5754d3ac00c36cc62166ddbef88c596a2fe737aac04baf111a843</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Animals</topic><topic>Beauveria - drug effects</topic><topic>Beauveria - physiology</topic><topic>Beauveria brongniartii</topic><topic>Biological and medical sciences</topic><topic>Biological control</topic><topic>Biomass</topic><topic>Control</topic><topic>Cryopreservation - methods</topic><topic>Cryoprotection</topic><topic>Cryoprotective Agents - pharmacology</topic><topic>Dextrans - pharmacology</topic><topic>Formulation</topic><topic>Freeze Drying - methods</topic><topic>Fructose - pharmacology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Imbibitional damage</topic><topic>Lyoprotection</topic><topic>Metarhizium - drug effects</topic><topic>Metarhizium - physiology</topic><topic>Metarhizium anisopliae</topic><topic>Pest Control, Biological - methods</topic><topic>Phytopathology. Animal pests. Plant and forest protection</topic><topic>Protozoa. Invertebrates</topic><topic>Spores, Fungal - drug effects</topic><topic>Spores, Fungal - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Toegel, Stefan</creatorcontrib><creatorcontrib>Salar-Behzadi, Sharareh</creatorcontrib><creatorcontrib>Horaczek-Clausen, Andrea</creatorcontrib><creatorcontrib>Viernstein, Helmut</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><jtitle>Journal of invertebrate pathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Toegel, Stefan</au><au>Salar-Behzadi, Sharareh</au><au>Horaczek-Clausen, Andrea</au><au>Viernstein, Helmut</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Preservation of aerial conidia and biomasses from entomopathogenic fungi Beauveria brongniartii and Metarhizium anisopliae during lyophilization</atitle><jtitle>Journal of invertebrate pathology</jtitle><addtitle>J Invertebr Pathol</addtitle><date>2010-09-01</date><risdate>2010</risdate><volume>105</volume><issue>1</issue><spage>16</spage><epage>23</epage><pages>16-23</pages><issn>0022-2011</issn><eissn>1096-0805</eissn><coden>JIVPAZ</coden><abstract>In this study, we assessed the stability provided by different formulations to aerial conidia or biomasses (conidia, blastospores, and mycelia) of
Beauveria brongniartii and
Metarhizium anisopliae subjected to lyophilization. First, the impact of the freezing and drying processes on spore survival was evaluated. Whereas unprotected
B. brongniartii spores showed high cryosensitivity, those of
M. anisopliae were markedly harmed by the drying process. Then, the protective efficiency of 14 excipients was systematically evaluated and optimized regarding required concentrations. Fructose, glucose, and saccharose significantly enhanced viabilities for
B. brongniartii and
M. anisopliae spores following lyophilization, especially as a result of their cryoprotective effects. In addition, the effect of various bulking agents on spore survival was studied and dextran 4 was selected to enhance the physical properties of the lyophilized products. The combination of fructose and dextran 4 was further applied to prepare lyophilized biomasses of both fungi. In comparison to freshly harvested biomasses, the lyophilized products showed similar growth rates and a comparable production of virulent secondary metabolites such as destruxin A, destruxin B, or oosporein, suggesting their applicability as biological control agents.</abstract><cop>Amsterdam</cop><pub>Elsevier Inc</pub><pmid>20457163</pmid><doi>10.1016/j.jip.2010.05.004</doi><tpages>8</tpages></addata></record> |
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| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Beauveria - drug effects Beauveria - physiology Beauveria brongniartii Biological and medical sciences Biological control Biomass Control Cryopreservation - methods Cryoprotection Cryoprotective Agents - pharmacology Dextrans - pharmacology Formulation Freeze Drying - methods Fructose - pharmacology Fundamental and applied biological sciences. Psychology Imbibitional damage Lyoprotection Metarhizium - drug effects Metarhizium - physiology Metarhizium anisopliae Pest Control, Biological - methods Phytopathology. Animal pests. Plant and forest protection Protozoa. Invertebrates Spores, Fungal - drug effects Spores, Fungal - physiology |
| title | Preservation of aerial conidia and biomasses from entomopathogenic fungi Beauveria brongniartii and Metarhizium anisopliae during lyophilization |
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