Frequency and functional activity of Th17, Tc17 and other T-cell subsets in Systemic Lupus Erythematosus
To compare frequency and functional activity of peripheral blood (PB) Th(c)17, Th(c)1 and Treg cells and the amount of type 2 cytokines mRNA we recruited SLE patients in active ( n = 15) and inactive disease ( n = 19) and healthy age- and gender-matched controls ( n = 15). The study of Th(c)17, Th(c...
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| Veröffentlicht in: | Cellular immunology 2010, Vol.264 (1), p.97-103 |
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| creator | Henriques, Ana Inês, Luís Couto, Maura Pedreiro, Susana Santos, Catarina Magalhães, Mariana Santos, Paulo Velada, Isabel Almeida, Anabela Carvalheiro, Tiago Laranjeira, Paula Morgado, José Mário Pais, Maria Luísa Silva, José António Pereira da Paiva, Artur |
| description | To compare frequency and functional activity of peripheral blood (PB) Th(c)17, Th(c)1 and Treg cells and the amount of type 2 cytokines mRNA we recruited SLE patients in active (
n
=
15) and inactive disease (
n
=
19) and healthy age- and gender-matched controls (
n
=
15). The study of Th(c)17, Th(c)1 and Treg cells was done by flow cytometry and cytokine mRNA by real-time PCR. Compared to NC, SLE patients present an increased proportion of Th(c)17 cells, but with lower amounts of IL-17
per cell and also a decreased frequency of Treg, but with increased production of TGF-β and FoxP3 mRNA. Ιn active compared to inactive SLE, there is a marked decreased in frequency of Th(c)1 cells, an increased production of type 2 cytokines mRNA and a distinct functional profile of Th(c)17 cells. Our findings suggest a functional disequilibrium of T-cell subsets in SLE which may contribute to the inflammatory process and disease pathogenesis. |
| doi_str_mv | 10.1016/j.cellimm.2010.05.004 |
| format | Article |
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n
=
15) and inactive disease (
n
=
19) and healthy age- and gender-matched controls (
n
=
15). The study of Th(c)17, Th(c)1 and Treg cells was done by flow cytometry and cytokine mRNA by real-time PCR. Compared to NC, SLE patients present an increased proportion of Th(c)17 cells, but with lower amounts of IL-17
per cell and also a decreased frequency of Treg, but with increased production of TGF-β and FoxP3 mRNA. Ιn active compared to inactive SLE, there is a marked decreased in frequency of Th(c)1 cells, an increased production of type 2 cytokines mRNA and a distinct functional profile of Th(c)17 cells. Our findings suggest a functional disequilibrium of T-cell subsets in SLE which may contribute to the inflammatory process and disease pathogenesis.</description><identifier>ISSN: 0008-8749</identifier><identifier>EISSN: 1090-2163</identifier><identifier>DOI: 10.1016/j.cellimm.2010.05.004</identifier><identifier>PMID: 20553755</identifier><language>eng</language><publisher>Netherlands: Elsevier Inc</publisher><subject>Adult ; Antigens, CD - biosynthesis ; Autoimmune disease ; Cell Count ; Cell Separation ; Cytokines ; Female ; Flow Cytometry ; Forkhead Transcription Factors - biosynthesis ; Forkhead Transcription Factors - genetics ; Gene Expression Regulation ; Humans ; Interleukin-17 - biosynthesis ; Interleukin-17 - genetics ; Lupus Erythematosus, Systemic - immunology ; Lupus Erythematosus, Systemic - pathology ; Male ; Regulatory T cells ; Reverse Transcriptase Polymerase Chain Reaction ; SLE ; T-Lymphocyte Subsets - immunology ; T-Lymphocyte Subsets - metabolism ; T-Lymphocyte Subsets - pathology ; T-Lymphocytes, Cytotoxic - immunology ; T-Lymphocytes, Cytotoxic - metabolism ; T-Lymphocytes, Cytotoxic - pathology ; T-Lymphocytes, Regulatory - immunology ; T-Lymphocytes, Regulatory - metabolism ; T-Lymphocytes, Regulatory - pathology ; Tc1 ; Tc17 ; Th1 ; Th17 ; Th2 Cells - immunology ; Th2 Cells - metabolism ; Th2 Cells - pathology ; Transforming Growth Factor beta - biosynthesis ; Transforming Growth Factor beta - genetics ; Transforming Growth Factor beta - secretion</subject><ispartof>Cellular immunology, 2010, Vol.264 (1), p.97-103</ispartof><rights>2010 Elsevier Inc.</rights><rights>2010 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c443t-e5820eb3bb5bb7caba429bbd5e91e84416b5126fede29179a434ad44bcec5b263</citedby><cites>FETCH-LOGICAL-c443t-e5820eb3bb5bb7caba429bbd5e91e84416b5126fede29179a434ad44bcec5b263</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0008874910001255$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,4010,27902,27903,27904,65309</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20553755$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Henriques, Ana</creatorcontrib><creatorcontrib>Inês, Luís</creatorcontrib><creatorcontrib>Couto, Maura</creatorcontrib><creatorcontrib>Pedreiro, Susana</creatorcontrib><creatorcontrib>Santos, Catarina</creatorcontrib><creatorcontrib>Magalhães, Mariana</creatorcontrib><creatorcontrib>Santos, Paulo</creatorcontrib><creatorcontrib>Velada, Isabel</creatorcontrib><creatorcontrib>Almeida, Anabela</creatorcontrib><creatorcontrib>Carvalheiro, Tiago</creatorcontrib><creatorcontrib>Laranjeira, Paula</creatorcontrib><creatorcontrib>Morgado, José Mário</creatorcontrib><creatorcontrib>Pais, Maria Luísa</creatorcontrib><creatorcontrib>Silva, José António Pereira da</creatorcontrib><creatorcontrib>Paiva, Artur</creatorcontrib><title>Frequency and functional activity of Th17, Tc17 and other T-cell subsets in Systemic Lupus Erythematosus</title><title>Cellular immunology</title><addtitle>Cell Immunol</addtitle><description>To compare frequency and functional activity of peripheral blood (PB) Th(c)17, Th(c)1 and Treg cells and the amount of type 2 cytokines mRNA we recruited SLE patients in active (
n
=
15) and inactive disease (
n
=
19) and healthy age- and gender-matched controls (
n
=
15). The study of Th(c)17, Th(c)1 and Treg cells was done by flow cytometry and cytokine mRNA by real-time PCR. Compared to NC, SLE patients present an increased proportion of Th(c)17 cells, but with lower amounts of IL-17
per cell and also a decreased frequency of Treg, but with increased production of TGF-β and FoxP3 mRNA. Ιn active compared to inactive SLE, there is a marked decreased in frequency of Th(c)1 cells, an increased production of type 2 cytokines mRNA and a distinct functional profile of Th(c)17 cells. Our findings suggest a functional disequilibrium of T-cell subsets in SLE which may contribute to the inflammatory process and disease pathogenesis.</description><subject>Adult</subject><subject>Antigens, CD - biosynthesis</subject><subject>Autoimmune disease</subject><subject>Cell Count</subject><subject>Cell Separation</subject><subject>Cytokines</subject><subject>Female</subject><subject>Flow Cytometry</subject><subject>Forkhead Transcription Factors - biosynthesis</subject><subject>Forkhead Transcription Factors - genetics</subject><subject>Gene Expression Regulation</subject><subject>Humans</subject><subject>Interleukin-17 - biosynthesis</subject><subject>Interleukin-17 - genetics</subject><subject>Lupus Erythematosus, Systemic - immunology</subject><subject>Lupus Erythematosus, Systemic - pathology</subject><subject>Male</subject><subject>Regulatory T cells</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>SLE</subject><subject>T-Lymphocyte Subsets - immunology</subject><subject>T-Lymphocyte Subsets - metabolism</subject><subject>T-Lymphocyte Subsets - pathology</subject><subject>T-Lymphocytes, Cytotoxic - immunology</subject><subject>T-Lymphocytes, Cytotoxic - metabolism</subject><subject>T-Lymphocytes, Cytotoxic - pathology</subject><subject>T-Lymphocytes, Regulatory - immunology</subject><subject>T-Lymphocytes, Regulatory - metabolism</subject><subject>T-Lymphocytes, Regulatory - pathology</subject><subject>Tc1</subject><subject>Tc17</subject><subject>Th1</subject><subject>Th17</subject><subject>Th2 Cells - immunology</subject><subject>Th2 Cells - metabolism</subject><subject>Th2 Cells - pathology</subject><subject>Transforming Growth Factor beta - biosynthesis</subject><subject>Transforming Growth Factor beta - genetics</subject><subject>Transforming Growth Factor beta - secretion</subject><issn>0008-8749</issn><issn>1090-2163</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1v0zAYgK2JaSuDnwDyjQvpXn8l8QmhaQOkShwoZ8t23qiumqTYzqT8-7m0cN3Jr6zn_XwI-cBgzYDV9_u1x8MhDMOaQ_kDtQaQV2TFQEPFWS3ekBUAtFXbSH1L3qa0B2BMarghtxyUEo1SK7J7ivhnxtEv1I4d7efR5zCN9kBtCZ5DXujU0-2ONZ_p1rPmLzXlHUa6rU4T0DS7hDnRMNJfS8o4BE8383FO9DEuBRxsntKc3pHr3h4Svr-8d-T30-P24Xu1-fntx8PXTeWlFLlC1XJAJ5xTzjXeOiu5dq5TqBm2UrLaKcbrHjvkmjXaSiFtJ6Xz6JXjtbgjn851j3Eqi6VshpBOg9oRpzmZlpXVudLtq2QjhG5BgCikOpM-TilF7M0xhsHGxTAwJxtmby42zMmGAWWKjZL38dJhdgN2_7P-nb8AX84Alos8B4wm-VBkYBci-my6KbzS4gUImZ5Z</recordid><startdate>2010</startdate><enddate>2010</enddate><creator>Henriques, Ana</creator><creator>Inês, Luís</creator><creator>Couto, Maura</creator><creator>Pedreiro, Susana</creator><creator>Santos, Catarina</creator><creator>Magalhães, Mariana</creator><creator>Santos, Paulo</creator><creator>Velada, Isabel</creator><creator>Almeida, Anabela</creator><creator>Carvalheiro, Tiago</creator><creator>Laranjeira, Paula</creator><creator>Morgado, José Mário</creator><creator>Pais, Maria Luísa</creator><creator>Silva, José António Pereira da</creator><creator>Paiva, Artur</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7T5</scope><scope>H94</scope></search><sort><creationdate>2010</creationdate><title>Frequency and functional activity of Th17, Tc17 and other T-cell subsets in Systemic Lupus Erythematosus</title><author>Henriques, Ana ; Inês, Luís ; Couto, Maura ; Pedreiro, Susana ; Santos, Catarina ; Magalhães, Mariana ; Santos, Paulo ; Velada, Isabel ; Almeida, Anabela ; Carvalheiro, Tiago ; Laranjeira, Paula ; Morgado, José Mário ; Pais, Maria Luísa ; Silva, José António Pereira da ; Paiva, Artur</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c443t-e5820eb3bb5bb7caba429bbd5e91e84416b5126fede29179a434ad44bcec5b263</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Adult</topic><topic>Antigens, CD - biosynthesis</topic><topic>Autoimmune disease</topic><topic>Cell Count</topic><topic>Cell Separation</topic><topic>Cytokines</topic><topic>Female</topic><topic>Flow Cytometry</topic><topic>Forkhead Transcription Factors - biosynthesis</topic><topic>Forkhead Transcription Factors - genetics</topic><topic>Gene Expression Regulation</topic><topic>Humans</topic><topic>Interleukin-17 - biosynthesis</topic><topic>Interleukin-17 - genetics</topic><topic>Lupus Erythematosus, Systemic - immunology</topic><topic>Lupus Erythematosus, Systemic - pathology</topic><topic>Male</topic><topic>Regulatory T cells</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>SLE</topic><topic>T-Lymphocyte Subsets - immunology</topic><topic>T-Lymphocyte Subsets - metabolism</topic><topic>T-Lymphocyte Subsets - pathology</topic><topic>T-Lymphocytes, Cytotoxic - immunology</topic><topic>T-Lymphocytes, Cytotoxic - metabolism</topic><topic>T-Lymphocytes, Cytotoxic - pathology</topic><topic>T-Lymphocytes, Regulatory - immunology</topic><topic>T-Lymphocytes, Regulatory - metabolism</topic><topic>T-Lymphocytes, Regulatory - pathology</topic><topic>Tc1</topic><topic>Tc17</topic><topic>Th1</topic><topic>Th17</topic><topic>Th2 Cells - immunology</topic><topic>Th2 Cells - metabolism</topic><topic>Th2 Cells - pathology</topic><topic>Transforming Growth Factor beta - biosynthesis</topic><topic>Transforming Growth Factor beta - genetics</topic><topic>Transforming Growth Factor beta - secretion</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Henriques, Ana</creatorcontrib><creatorcontrib>Inês, Luís</creatorcontrib><creatorcontrib>Couto, Maura</creatorcontrib><creatorcontrib>Pedreiro, Susana</creatorcontrib><creatorcontrib>Santos, Catarina</creatorcontrib><creatorcontrib>Magalhães, Mariana</creatorcontrib><creatorcontrib>Santos, Paulo</creatorcontrib><creatorcontrib>Velada, Isabel</creatorcontrib><creatorcontrib>Almeida, Anabela</creatorcontrib><creatorcontrib>Carvalheiro, Tiago</creatorcontrib><creatorcontrib>Laranjeira, Paula</creatorcontrib><creatorcontrib>Morgado, José Mário</creatorcontrib><creatorcontrib>Pais, Maria Luísa</creatorcontrib><creatorcontrib>Silva, José António Pereira da</creatorcontrib><creatorcontrib>Paiva, Artur</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><jtitle>Cellular immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Henriques, Ana</au><au>Inês, Luís</au><au>Couto, Maura</au><au>Pedreiro, Susana</au><au>Santos, Catarina</au><au>Magalhães, Mariana</au><au>Santos, Paulo</au><au>Velada, Isabel</au><au>Almeida, Anabela</au><au>Carvalheiro, Tiago</au><au>Laranjeira, Paula</au><au>Morgado, José Mário</au><au>Pais, Maria Luísa</au><au>Silva, José António Pereira da</au><au>Paiva, Artur</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Frequency and functional activity of Th17, Tc17 and other T-cell subsets in Systemic Lupus Erythematosus</atitle><jtitle>Cellular immunology</jtitle><addtitle>Cell Immunol</addtitle><date>2010</date><risdate>2010</risdate><volume>264</volume><issue>1</issue><spage>97</spage><epage>103</epage><pages>97-103</pages><issn>0008-8749</issn><eissn>1090-2163</eissn><abstract>To compare frequency and functional activity of peripheral blood (PB) Th(c)17, Th(c)1 and Treg cells and the amount of type 2 cytokines mRNA we recruited SLE patients in active (
n
=
15) and inactive disease (
n
=
19) and healthy age- and gender-matched controls (
n
=
15). The study of Th(c)17, Th(c)1 and Treg cells was done by flow cytometry and cytokine mRNA by real-time PCR. Compared to NC, SLE patients present an increased proportion of Th(c)17 cells, but with lower amounts of IL-17
per cell and also a decreased frequency of Treg, but with increased production of TGF-β and FoxP3 mRNA. Ιn active compared to inactive SLE, there is a marked decreased in frequency of Th(c)1 cells, an increased production of type 2 cytokines mRNA and a distinct functional profile of Th(c)17 cells. Our findings suggest a functional disequilibrium of T-cell subsets in SLE which may contribute to the inflammatory process and disease pathogenesis.</abstract><cop>Netherlands</cop><pub>Elsevier Inc</pub><pmid>20553755</pmid><doi>10.1016/j.cellimm.2010.05.004</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
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| ispartof | Cellular immunology, 2010, Vol.264 (1), p.97-103 |
| issn | 0008-8749 1090-2163 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Adult Antigens, CD - biosynthesis Autoimmune disease Cell Count Cell Separation Cytokines Female Flow Cytometry Forkhead Transcription Factors - biosynthesis Forkhead Transcription Factors - genetics Gene Expression Regulation Humans Interleukin-17 - biosynthesis Interleukin-17 - genetics Lupus Erythematosus, Systemic - immunology Lupus Erythematosus, Systemic - pathology Male Regulatory T cells Reverse Transcriptase Polymerase Chain Reaction SLE T-Lymphocyte Subsets - immunology T-Lymphocyte Subsets - metabolism T-Lymphocyte Subsets - pathology T-Lymphocytes, Cytotoxic - immunology T-Lymphocytes, Cytotoxic - metabolism T-Lymphocytes, Cytotoxic - pathology T-Lymphocytes, Regulatory - immunology T-Lymphocytes, Regulatory - metabolism T-Lymphocytes, Regulatory - pathology Tc1 Tc17 Th1 Th17 Th2 Cells - immunology Th2 Cells - metabolism Th2 Cells - pathology Transforming Growth Factor beta - biosynthesis Transforming Growth Factor beta - genetics Transforming Growth Factor beta - secretion |
| title | Frequency and functional activity of Th17, Tc17 and other T-cell subsets in Systemic Lupus Erythematosus |
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