Steroid biosynthesis in vitro by the gonads of Sparus auratus L. (Teleostei) at different stages during natural sex reversal
The metabolism in vitro of pregnenolone-4- 14C by the gonads of Sparus auratus L., a protandrous hermaphroditic teleost, was studied during sexual maturity in the male and female phases and during sex reversal. Steroids were identified by their mobilities on thin-layer chromatograms, derivative form...
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| Veröffentlicht in: | General and comparative endocrinology 1972-08, Vol.19 (1), p.26-36 |
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| creator | Colombo, Lorenzo Del Conte, Emilia Clemenze, Pierandrea |
| description | The metabolism
in vitro of pregnenolone-4-
14C by the gonads of
Sparus auratus L., a protandrous hermaphroditic teleost, was studied during sexual maturity in the male and female phases and during sex reversal. Steroids were identified by their mobilities on thin-layer chromatograms, derivative formation, and recrystallization to constant specific activity, and their yield-time curves were plotted. Progesterone and 17α-hydroxyprogesterone were in every case the most abundant metabolites isolated, and the only ones identified in incubates with ovarian tissue during sex reversal. Some androstenedione was produced by mature testicular tissue, testicular tissue during reversal, and mature ovarian tissue, but only in the first case was there a significant further conversion to testosterone. Although estrogen production was always very small, estradiol-17β and estrone were isolated. Their formation was greater in mature ovarian tissue than in testicular preparations. The gonadal steroids of
Sparus auratus L. do not seem to differ from those already described in various gonochoristic species. During sex reversal, gonadal steroid biosynthesis is probably at a minimum, with little interaction between the coexisting testicular and ovarian tissues. |
| doi_str_mv | 10.1016/0016-6480(72)90003-2 |
| format | Article |
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in vitro of pregnenolone-4-
14C by the gonads of
Sparus auratus L., a protandrous hermaphroditic teleost, was studied during sexual maturity in the male and female phases and during sex reversal. Steroids were identified by their mobilities on thin-layer chromatograms, derivative formation, and recrystallization to constant specific activity, and their yield-time curves were plotted. Progesterone and 17α-hydroxyprogesterone were in every case the most abundant metabolites isolated, and the only ones identified in incubates with ovarian tissue during sex reversal. Some androstenedione was produced by mature testicular tissue, testicular tissue during reversal, and mature ovarian tissue, but only in the first case was there a significant further conversion to testosterone. Although estrogen production was always very small, estradiol-17β and estrone were isolated. Their formation was greater in mature ovarian tissue than in testicular preparations. The gonadal steroids of
Sparus auratus L. do not seem to differ from those already described in various gonochoristic species. During sex reversal, gonadal steroid biosynthesis is probably at a minimum, with little interaction between the coexisting testicular and ovarian tissues.</description><identifier>ISSN: 0016-6480</identifier><identifier>EISSN: 1095-6840</identifier><identifier>DOI: 10.1016/0016-6480(72)90003-2</identifier><identifier>PMID: 5047342</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject><![CDATA[17-Ketosteroids - isolation & purification ; Androstanes - isolation & purification ; Animals ; Carbon Isotopes ; Chromatography, Thin Layer ; Crystallization ; Disorders of Sex Development - metabolism ; Estradiol - isolation & purification ; Estrone - isolation & purification ; Female ; Fishes - metabolism ; Gonads - metabolism ; Hydroxyprogesterones - isolation & purification ; In Vitro Techniques ; Male ; Ovary - metabolism ; Pregnenolone - metabolism ; Progesterone - isolation & purification ; Testosterone - isolation & purification ; Tritium]]></subject><ispartof>General and comparative endocrinology, 1972-08, Vol.19 (1), p.26-36</ispartof><rights>1972</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c272t-5254e45f730a3ccb2873f448b52c31216c9ec491a962fa0015fb77cef3d78e0d3</citedby><cites>FETCH-LOGICAL-c272t-5254e45f730a3ccb2873f448b52c31216c9ec491a962fa0015fb77cef3d78e0d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0016-6480(72)90003-2$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/5047342$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Colombo, Lorenzo</creatorcontrib><creatorcontrib>Del Conte, Emilia</creatorcontrib><creatorcontrib>Clemenze, Pierandrea</creatorcontrib><title>Steroid biosynthesis in vitro by the gonads of Sparus auratus L. (Teleostei) at different stages during natural sex reversal</title><title>General and comparative endocrinology</title><addtitle>Gen Comp Endocrinol</addtitle><description>The metabolism
in vitro of pregnenolone-4-
14C by the gonads of
Sparus auratus L., a protandrous hermaphroditic teleost, was studied during sexual maturity in the male and female phases and during sex reversal. Steroids were identified by their mobilities on thin-layer chromatograms, derivative formation, and recrystallization to constant specific activity, and their yield-time curves were plotted. Progesterone and 17α-hydroxyprogesterone were in every case the most abundant metabolites isolated, and the only ones identified in incubates with ovarian tissue during sex reversal. Some androstenedione was produced by mature testicular tissue, testicular tissue during reversal, and mature ovarian tissue, but only in the first case was there a significant further conversion to testosterone. Although estrogen production was always very small, estradiol-17β and estrone were isolated. Their formation was greater in mature ovarian tissue than in testicular preparations. The gonadal steroids of
Sparus auratus L. do not seem to differ from those already described in various gonochoristic species. During sex reversal, gonadal steroid biosynthesis is probably at a minimum, with little interaction between the coexisting testicular and ovarian tissues.</description><subject>17-Ketosteroids - isolation & purification</subject><subject>Androstanes - isolation & purification</subject><subject>Animals</subject><subject>Carbon Isotopes</subject><subject>Chromatography, Thin Layer</subject><subject>Crystallization</subject><subject>Disorders of Sex Development - metabolism</subject><subject>Estradiol - isolation & purification</subject><subject>Estrone - isolation & purification</subject><subject>Female</subject><subject>Fishes - metabolism</subject><subject>Gonads - metabolism</subject><subject>Hydroxyprogesterones - isolation & purification</subject><subject>In Vitro Techniques</subject><subject>Male</subject><subject>Ovary - metabolism</subject><subject>Pregnenolone - metabolism</subject><subject>Progesterone - isolation & purification</subject><subject>Testosterone - isolation & purification</subject><subject>Tritium</subject><issn>0016-6480</issn><issn>1095-6840</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1972</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kEuLFDEQgIMo6-zqP1DISXYPvebZj4sgi67CgIddzyGdVMZIT2dMpQcH_PFmnGGPXqqg6qsq6iPkDWe3nPH2PauhaVXPrjtxMzDGZCOekRVng27aXrHnZPWEvCSXiD8ro2XLL8iFZqqTSqzIn4cCOUVPx5jwMJcfgBFpnOk-lpzoeKC1RDdpth5pCvRhZ_OC1C7ZlprXt_T6ESZIWCDeUFuojyFAhrlQLHYDSP2S47yhc-WznSjCb5phDxnt9Iq8CHZCeH3OV-T750-Pd1-a9bf7r3cf140TnSiNFlqB0qGTzErnRtF3MijVj1o4yQVv3QBODdwOrQi2_qzD2HUOgvRdD8zLK_LutHeX068FsJhtRAfTZGdIC5qea9mrVlVQnUCXE2KGYHY5bm0-GM7MUbo5GjVHo6YT5p90I-rY2_P-ZdyCfxo6W679D6c-1Cf3EbJBF2F24GMGV4xP8f8H_gJZN5HE</recordid><startdate>197208</startdate><enddate>197208</enddate><creator>Colombo, Lorenzo</creator><creator>Del Conte, Emilia</creator><creator>Clemenze, Pierandrea</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>197208</creationdate><title>Steroid biosynthesis in vitro by the gonads of Sparus auratus L. (Teleostei) at different stages during natural sex reversal</title><author>Colombo, Lorenzo ; Del Conte, Emilia ; Clemenze, Pierandrea</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c272t-5254e45f730a3ccb2873f448b52c31216c9ec491a962fa0015fb77cef3d78e0d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1972</creationdate><topic>17-Ketosteroids - isolation & purification</topic><topic>Androstanes - isolation & purification</topic><topic>Animals</topic><topic>Carbon Isotopes</topic><topic>Chromatography, Thin Layer</topic><topic>Crystallization</topic><topic>Disorders of Sex Development - metabolism</topic><topic>Estradiol - isolation & purification</topic><topic>Estrone - isolation & purification</topic><topic>Female</topic><topic>Fishes - metabolism</topic><topic>Gonads - metabolism</topic><topic>Hydroxyprogesterones - isolation & purification</topic><topic>In Vitro Techniques</topic><topic>Male</topic><topic>Ovary - metabolism</topic><topic>Pregnenolone - metabolism</topic><topic>Progesterone - isolation & purification</topic><topic>Testosterone - isolation & purification</topic><topic>Tritium</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Colombo, Lorenzo</creatorcontrib><creatorcontrib>Del Conte, Emilia</creatorcontrib><creatorcontrib>Clemenze, Pierandrea</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>General and comparative endocrinology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Colombo, Lorenzo</au><au>Del Conte, Emilia</au><au>Clemenze, Pierandrea</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Steroid biosynthesis in vitro by the gonads of Sparus auratus L. (Teleostei) at different stages during natural sex reversal</atitle><jtitle>General and comparative endocrinology</jtitle><addtitle>Gen Comp Endocrinol</addtitle><date>1972-08</date><risdate>1972</risdate><volume>19</volume><issue>1</issue><spage>26</spage><epage>36</epage><pages>26-36</pages><issn>0016-6480</issn><eissn>1095-6840</eissn><abstract>The metabolism
in vitro of pregnenolone-4-
14C by the gonads of
Sparus auratus L., a protandrous hermaphroditic teleost, was studied during sexual maturity in the male and female phases and during sex reversal. Steroids were identified by their mobilities on thin-layer chromatograms, derivative formation, and recrystallization to constant specific activity, and their yield-time curves were plotted. Progesterone and 17α-hydroxyprogesterone were in every case the most abundant metabolites isolated, and the only ones identified in incubates with ovarian tissue during sex reversal. Some androstenedione was produced by mature testicular tissue, testicular tissue during reversal, and mature ovarian tissue, but only in the first case was there a significant further conversion to testosterone. Although estrogen production was always very small, estradiol-17β and estrone were isolated. Their formation was greater in mature ovarian tissue than in testicular preparations. The gonadal steroids of
Sparus auratus L. do not seem to differ from those already described in various gonochoristic species. During sex reversal, gonadal steroid biosynthesis is probably at a minimum, with little interaction between the coexisting testicular and ovarian tissues.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>5047342</pmid><doi>10.1016/0016-6480(72)90003-2</doi><tpages>11</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | General and comparative endocrinology, 1972-08, Vol.19 (1), p.26-36 |
| issn | 0016-6480 1095-6840 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_81538464 |
| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | 17-Ketosteroids - isolation & purification Androstanes - isolation & purification Animals Carbon Isotopes Chromatography, Thin Layer Crystallization Disorders of Sex Development - metabolism Estradiol - isolation & purification Estrone - isolation & purification Female Fishes - metabolism Gonads - metabolism Hydroxyprogesterones - isolation & purification In Vitro Techniques Male Ovary - metabolism Pregnenolone - metabolism Progesterone - isolation & purification Testosterone - isolation & purification Tritium |
| title | Steroid biosynthesis in vitro by the gonads of Sparus auratus L. (Teleostei) at different stages during natural sex reversal |
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