Studies on the Glucaric Acid Pathway in the Metabolism of D-Glucuronic Acid in Mammals. IV. Fluorometric Method for the Determination of D-Glucaric Acid in Serum
In connection with the biochemical studies on D-glucaric acid, a normal constituent of human urine which is derived from D-glucuronolactone, we developed a colorimetric method for the quantitative determination of this acid. Thus, D-glucaric acid separated from mammalian urines using ion-exchange co...
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| Veröffentlicht in: | Chemical & pharmaceutical bulletin 1972/04/25, Vol.20(4), pp.845-848 |
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| container_title | Chemical & pharmaceutical bulletin |
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| creator | MATSUI, MICHIO ABE, FUKUO WATANABE, YASUYO WANIBE, TAMAE OKADA, MASASHI |
| description | In connection with the biochemical studies on D-glucaric acid, a normal constituent of human urine which is derived from D-glucuronolactone, we developed a colorimetric method for the quantitative determination of this acid. Thus, D-glucaric acid separated from mammalian urines using ion-exchange column chromatography was oxidized with periodic acid to give glyoxylic acid, which was further converted into the intensely colored 1, 5-diphenyl-formazan and determined colorimetrically. During the course of our studies on D-glucaric acid a much more highly sensitive method was required for the accurate estimation of normal serum level of D-glucaric acid in mammals. The present paper deals with the fluorometric determination of D-glucaric acid which is based on the condensation of glyoxylic acid derived from D-glucaric acid with 4'-hydrazino-2-stilbazole to yield a highly fluorescent product using a modification of the procedure for the determination of α-oxo acids originally reported by Mizutani, et al.(Fig. 1). |
| doi_str_mv | 10.1248/cpb.20.845 |
| format | Article |
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During the course of our studies on D-glucaric acid a much more highly sensitive method was required for the accurate estimation of normal serum level of D-glucaric acid in mammals. The present paper deals with the fluorometric determination of D-glucaric acid which is based on the condensation of glyoxylic acid derived from D-glucaric acid with 4'-hydrazino-2-stilbazole to yield a highly fluorescent product using a modification of the procedure for the determination of α-oxo acids originally reported by Mizutani, et al.(Fig. 1).</description><identifier>ISSN: 0009-2363</identifier><identifier>EISSN: 1347-5223</identifier><identifier>DOI: 10.1248/cpb.20.845</identifier><identifier>PMID: 5065229</identifier><language>eng</language><publisher>Japan: The Pharmaceutical Society of Japan</publisher><subject>Animals ; Fluorometry ; Glucuronates - blood ; Humans ; Hydrazines ; Hydrogen ; Male ; Methods ; Potassium ; Pyridines ; Rats</subject><ispartof>Chemical and Pharmaceutical Bulletin, 1972/04/25, Vol.20(4), pp.845-848</ispartof><rights>The Pharmaceutical Society of Japan</rights><rights>Copyright Japan Science and Technology Agency 1972</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5175-f85f25102a608f662e59b06b19ef7c0afdd5e36b588e197af5e4b77ef802b643</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,1881,4022,27921,27922,27923</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/5065229$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>MATSUI, MICHIO</creatorcontrib><creatorcontrib>ABE, FUKUO</creatorcontrib><creatorcontrib>WATANABE, YASUYO</creatorcontrib><creatorcontrib>WANIBE, TAMAE</creatorcontrib><creatorcontrib>OKADA, MASASHI</creatorcontrib><title>Studies on the Glucaric Acid Pathway in the Metabolism of D-Glucuronic Acid in Mammals. IV. Fluorometric Method for the Determination of D-Glucaric Acid in Serum</title><title>Chemical & pharmaceutical bulletin</title><addtitle>Chem. Pharm. Bull.</addtitle><description>In connection with the biochemical studies on D-glucaric acid, a normal constituent of human urine which is derived from D-glucuronolactone, we developed a colorimetric method for the quantitative determination of this acid. Thus, D-glucaric acid separated from mammalian urines using ion-exchange column chromatography was oxidized with periodic acid to give glyoxylic acid, which was further converted into the intensely colored 1, 5-diphenyl-formazan and determined colorimetrically. During the course of our studies on D-glucaric acid a much more highly sensitive method was required for the accurate estimation of normal serum level of D-glucaric acid in mammals. The present paper deals with the fluorometric determination of D-glucaric acid which is based on the condensation of glyoxylic acid derived from D-glucaric acid with 4'-hydrazino-2-stilbazole to yield a highly fluorescent product using a modification of the procedure for the determination of α-oxo acids originally reported by Mizutani, et al.(Fig. 1).</description><subject>Animals</subject><subject>Fluorometry</subject><subject>Glucuronates - blood</subject><subject>Humans</subject><subject>Hydrazines</subject><subject>Hydrogen</subject><subject>Male</subject><subject>Methods</subject><subject>Potassium</subject><subject>Pyridines</subject><subject>Rats</subject><issn>0009-2363</issn><issn>1347-5223</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1972</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkU2L1TAUhoMo43V0414ICC6E1ny26XK444wDMyjM4Dak7Yk3l7a5JikyP8d_amqvd8DNOYv3yXMCL0JvKSkpE-pTd2hLRkol5DO0oVzUhWSMP0cbQkhTMF7xl-hVjHtCmCQ1P0NnklQZaTbo932aewcR-wmnHeDrYe5McB2-6FyPv5m0-2UesVvDO0im9YOLI_YWXxYLPAc__cMzdmfG0QyxxDffS3w1zD74EdIizI93vsfWh7-uS0gQRjeZ5PLpk-7pdpbdQ5jH1-iFzUZ4c9zn6OHq88P2S3H79fpme3FbdJLWsrBKWiYpYaYiylYVA9m0pGppA7buiLF9L4FXrVQKaFMbK0G0dQ1WEdZWgp-jD6v2EPzPGWLSo4sdDIOZwM9RKyo5o4Jn8P1_4N7PYcpf01TIppaNqJpMfVypLvgYA1h9CG404VFTopfSdC5NM6JzaRl-d1TO7Qj9CT22lPPtmu9jMj_glJuQXDfAoqKNVItOrCNbn9KdCRom_ge5-amP</recordid><startdate>1972</startdate><enddate>1972</enddate><creator>MATSUI, MICHIO</creator><creator>ABE, FUKUO</creator><creator>WATANABE, YASUYO</creator><creator>WANIBE, TAMAE</creator><creator>OKADA, MASASHI</creator><general>The Pharmaceutical Society of Japan</general><general>Japan Science and Technology Agency</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>1972</creationdate><title>Studies on the Glucaric Acid Pathway in the Metabolism of D-Glucuronic Acid in Mammals. IV. Fluorometric Method for the Determination of D-Glucaric Acid in Serum</title><author>MATSUI, MICHIO ; ABE, FUKUO ; WATANABE, YASUYO ; WANIBE, TAMAE ; OKADA, MASASHI</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5175-f85f25102a608f662e59b06b19ef7c0afdd5e36b588e197af5e4b77ef802b643</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1972</creationdate><topic>Animals</topic><topic>Fluorometry</topic><topic>Glucuronates - blood</topic><topic>Humans</topic><topic>Hydrazines</topic><topic>Hydrogen</topic><topic>Male</topic><topic>Methods</topic><topic>Potassium</topic><topic>Pyridines</topic><topic>Rats</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>MATSUI, MICHIO</creatorcontrib><creatorcontrib>ABE, FUKUO</creatorcontrib><creatorcontrib>WATANABE, YASUYO</creatorcontrib><creatorcontrib>WANIBE, TAMAE</creatorcontrib><creatorcontrib>OKADA, MASASHI</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Chemical & pharmaceutical bulletin</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>MATSUI, MICHIO</au><au>ABE, FUKUO</au><au>WATANABE, YASUYO</au><au>WANIBE, TAMAE</au><au>OKADA, MASASHI</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Studies on the Glucaric Acid Pathway in the Metabolism of D-Glucuronic Acid in Mammals. IV. Fluorometric Method for the Determination of D-Glucaric Acid in Serum</atitle><jtitle>Chemical & pharmaceutical bulletin</jtitle><addtitle>Chem. Pharm. Bull.</addtitle><date>1972</date><risdate>1972</risdate><volume>20</volume><issue>4</issue><spage>845</spage><epage>848</epage><pages>845-848</pages><issn>0009-2363</issn><eissn>1347-5223</eissn><abstract>In connection with the biochemical studies on D-glucaric acid, a normal constituent of human urine which is derived from D-glucuronolactone, we developed a colorimetric method for the quantitative determination of this acid. Thus, D-glucaric acid separated from mammalian urines using ion-exchange column chromatography was oxidized with periodic acid to give glyoxylic acid, which was further converted into the intensely colored 1, 5-diphenyl-formazan and determined colorimetrically. During the course of our studies on D-glucaric acid a much more highly sensitive method was required for the accurate estimation of normal serum level of D-glucaric acid in mammals. The present paper deals with the fluorometric determination of D-glucaric acid which is based on the condensation of glyoxylic acid derived from D-glucaric acid with 4'-hydrazino-2-stilbazole to yield a highly fluorescent product using a modification of the procedure for the determination of α-oxo acids originally reported by Mizutani, et al.(Fig. 1).</abstract><cop>Japan</cop><pub>The Pharmaceutical Society of Japan</pub><pmid>5065229</pmid><doi>10.1248/cpb.20.845</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Chemical and Pharmaceutical Bulletin, 1972/04/25, Vol.20(4), pp.845-848 |
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| source | J-STAGE Free; MEDLINE; EZB-FREE-00999 freely available EZB journals; Free Full-Text Journals in Chemistry |
| subjects | Animals Fluorometry Glucuronates - blood Humans Hydrazines Hydrogen Male Methods Potassium Pyridines Rats |
| title | Studies on the Glucaric Acid Pathway in the Metabolism of D-Glucuronic Acid in Mammals. IV. Fluorometric Method for the Determination of D-Glucaric Acid in Serum |
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