Direct colorimetric determination of phosphorus in serum and urine
A simple, sensitive and reliable method is presented for the direct photometric measurement of inorganic phosphate in serum or urine. Reagents are stable, inexpensive and minimal in number. The phosphomolybdate complex formed in an acid medium is reduced by p-methylaminophenol sulfate ; subsequent a...
Gespeichert in:
| Veröffentlicht in: | Clinica chimica acta 1972-06, Vol.39 (1), p.81-88 |
|---|---|
| 1. Verfasser: | |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 88 |
|---|---|
| container_issue | 1 |
| container_start_page | 81 |
| container_title | Clinica chimica acta |
| container_volume | 39 |
| creator | Drewes, Patricia A. |
| description | A simple, sensitive and reliable method is presented for the direct photometric measurement of inorganic phosphate in serum or urine. Reagents are stable, inexpensive and minimal in number. The phosphomolybdate complex formed in an acid medium is reduced by
p-methylaminophenol sulfate ; subsequent alkalinization with monoethanolamine produces a clear blue color, which is read at 660 nm. Results compare favorably with the Goldenberg-Fernandez manual method, which requires prior serum deproteinization, and the Kessler automated method. Inter-run precision is ± 3% (2 C.V.%). Bilirubin interference is slight and minimal hemolysis can be tolerated. Elevated serum protein levels do not interfere with the method. |
| doi_str_mv | 10.1016/0009-8981(72)90302-6 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_81502074</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>0009898172903026</els_id><sourcerecordid>81502074</sourcerecordid><originalsourceid>FETCH-LOGICAL-c423t-c8a2db9b66447bb1ca391c488a4aaa7bab975ca770d719e6edff64946316745a3</originalsourceid><addsrcrecordid>eNp9kE1LxDAQhoMo67r6DxRyEj1UkybNx0XQ9RMWvOg5pMkUI9tmTVrBf2_3A48ehmGY932HeRA6peSKEiquCSG6UFrRC1leasJIWYg9NKVKsoJxXe6j6Z_kEB3l_DmOnAg6QZOKMCWFmKK7-5DA9djFZUyhhT4Fhz30kNrQ2T7EDscGrz5iHisNGYcOZ0hDi23n8ZBCB8fooLHLDCe7PkPvjw9v8-di8fr0Mr9dFI6XrC-csqWvdS0E57KuqbNMU8eVstxaK2tba1k5KyXxkmoQ4JtGcM0Fo0LyyrIZOt_mrlL8GiD3pg3ZwXJpO4hDNopWpCSSj0K-FboUc07QmNX4mk0_hhKzRmfWXMyai5Gl2aAzYrSd7fKHugX_Z9qxGvc32z2MT34HSCa7AJ0Dv0FofAz_H_gFBol90g</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>81502074</pqid></control><display><type>article</type><title>Direct colorimetric determination of phosphorus in serum and urine</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Drewes, Patricia A.</creator><creatorcontrib>Drewes, Patricia A.</creatorcontrib><description>A simple, sensitive and reliable method is presented for the direct photometric measurement of inorganic phosphate in serum or urine. Reagents are stable, inexpensive and minimal in number. The phosphomolybdate complex formed in an acid medium is reduced by
p-methylaminophenol sulfate ; subsequent alkalinization with monoethanolamine produces a clear blue color, which is read at 660 nm. Results compare favorably with the Goldenberg-Fernandez manual method, which requires prior serum deproteinization, and the Kessler automated method. Inter-run precision is ± 3% (2 C.V.%). Bilirubin interference is slight and minimal hemolysis can be tolerated. Elevated serum protein levels do not interfere with the method.</description><identifier>ISSN: 0009-8981</identifier><identifier>EISSN: 1873-3492</identifier><identifier>DOI: 10.1016/0009-8981(72)90302-6</identifier><identifier>PMID: 5038766</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Aniline Compounds ; Bilirubin ; Blood Proteins ; Colorimetry ; Hemolysis ; Hydrogen-Ion Concentration ; Methods ; Molybdenum ; Phenols ; Phosphorus - analysis ; Phosphorus - blood ; Phosphorus - urine ; Sulfuric Acids</subject><ispartof>Clinica chimica acta, 1972-06, Vol.39 (1), p.81-88</ispartof><rights>1972</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c423t-c8a2db9b66447bb1ca391c488a4aaa7bab975ca770d719e6edff64946316745a3</citedby><cites>FETCH-LOGICAL-c423t-c8a2db9b66447bb1ca391c488a4aaa7bab975ca770d719e6edff64946316745a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0009898172903026$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/5038766$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Drewes, Patricia A.</creatorcontrib><title>Direct colorimetric determination of phosphorus in serum and urine</title><title>Clinica chimica acta</title><addtitle>Clin Chim Acta</addtitle><description>A simple, sensitive and reliable method is presented for the direct photometric measurement of inorganic phosphate in serum or urine. Reagents are stable, inexpensive and minimal in number. The phosphomolybdate complex formed in an acid medium is reduced by
p-methylaminophenol sulfate ; subsequent alkalinization with monoethanolamine produces a clear blue color, which is read at 660 nm. Results compare favorably with the Goldenberg-Fernandez manual method, which requires prior serum deproteinization, and the Kessler automated method. Inter-run precision is ± 3% (2 C.V.%). Bilirubin interference is slight and minimal hemolysis can be tolerated. Elevated serum protein levels do not interfere with the method.</description><subject>Aniline Compounds</subject><subject>Bilirubin</subject><subject>Blood Proteins</subject><subject>Colorimetry</subject><subject>Hemolysis</subject><subject>Hydrogen-Ion Concentration</subject><subject>Methods</subject><subject>Molybdenum</subject><subject>Phenols</subject><subject>Phosphorus - analysis</subject><subject>Phosphorus - blood</subject><subject>Phosphorus - urine</subject><subject>Sulfuric Acids</subject><issn>0009-8981</issn><issn>1873-3492</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1972</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1LxDAQhoMo67r6DxRyEj1UkybNx0XQ9RMWvOg5pMkUI9tmTVrBf2_3A48ehmGY932HeRA6peSKEiquCSG6UFrRC1leasJIWYg9NKVKsoJxXe6j6Z_kEB3l_DmOnAg6QZOKMCWFmKK7-5DA9djFZUyhhT4Fhz30kNrQ2T7EDscGrz5iHisNGYcOZ0hDi23n8ZBCB8fooLHLDCe7PkPvjw9v8-di8fr0Mr9dFI6XrC-csqWvdS0E57KuqbNMU8eVstxaK2tba1k5KyXxkmoQ4JtGcM0Fo0LyyrIZOt_mrlL8GiD3pg3ZwXJpO4hDNopWpCSSj0K-FboUc07QmNX4mk0_hhKzRmfWXMyai5Gl2aAzYrSd7fKHugX_Z9qxGvc32z2MT34HSCa7AJ0Dv0FofAz_H_gFBol90g</recordid><startdate>197206</startdate><enddate>197206</enddate><creator>Drewes, Patricia A.</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>197206</creationdate><title>Direct colorimetric determination of phosphorus in serum and urine</title><author>Drewes, Patricia A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c423t-c8a2db9b66447bb1ca391c488a4aaa7bab975ca770d719e6edff64946316745a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1972</creationdate><topic>Aniline Compounds</topic><topic>Bilirubin</topic><topic>Blood Proteins</topic><topic>Colorimetry</topic><topic>Hemolysis</topic><topic>Hydrogen-Ion Concentration</topic><topic>Methods</topic><topic>Molybdenum</topic><topic>Phenols</topic><topic>Phosphorus - analysis</topic><topic>Phosphorus - blood</topic><topic>Phosphorus - urine</topic><topic>Sulfuric Acids</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Drewes, Patricia A.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Clinica chimica acta</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Drewes, Patricia A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Direct colorimetric determination of phosphorus in serum and urine</atitle><jtitle>Clinica chimica acta</jtitle><addtitle>Clin Chim Acta</addtitle><date>1972-06</date><risdate>1972</risdate><volume>39</volume><issue>1</issue><spage>81</spage><epage>88</epage><pages>81-88</pages><issn>0009-8981</issn><eissn>1873-3492</eissn><abstract>A simple, sensitive and reliable method is presented for the direct photometric measurement of inorganic phosphate in serum or urine. Reagents are stable, inexpensive and minimal in number. The phosphomolybdate complex formed in an acid medium is reduced by
p-methylaminophenol sulfate ; subsequent alkalinization with monoethanolamine produces a clear blue color, which is read at 660 nm. Results compare favorably with the Goldenberg-Fernandez manual method, which requires prior serum deproteinization, and the Kessler automated method. Inter-run precision is ± 3% (2 C.V.%). Bilirubin interference is slight and minimal hemolysis can be tolerated. Elevated serum protein levels do not interfere with the method.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>5038766</pmid><doi>10.1016/0009-8981(72)90302-6</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0009-8981 |
| ispartof | Clinica chimica acta, 1972-06, Vol.39 (1), p.81-88 |
| issn | 0009-8981 1873-3492 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_81502074 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Aniline Compounds Bilirubin Blood Proteins Colorimetry Hemolysis Hydrogen-Ion Concentration Methods Molybdenum Phenols Phosphorus - analysis Phosphorus - blood Phosphorus - urine Sulfuric Acids |
| title | Direct colorimetric determination of phosphorus in serum and urine |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-06T11%3A55%3A18IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Direct%20colorimetric%20determination%20of%20phosphorus%20in%20serum%20and%20urine&rft.jtitle=Clinica%20chimica%20acta&rft.au=Drewes,%20Patricia%20A.&rft.date=1972-06&rft.volume=39&rft.issue=1&rft.spage=81&rft.epage=88&rft.pages=81-88&rft.issn=0009-8981&rft.eissn=1873-3492&rft_id=info:doi/10.1016/0009-8981(72)90302-6&rft_dat=%3Cproquest_cross%3E81502074%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=81502074&rft_id=info:pmid/5038766&rft_els_id=0009898172903026&rfr_iscdi=true |