Radiochemical microassay of δ-aminolevulinic acid synthetase in hepatic and erythroid tissues

A specific radiochemical microassay for δ-aminolevulinic acid synthetase is described. The assay is rapid and 10 to 500 times more sensitive than currently available methods. The assay measures the incorporation of radioactive succinate into δ-aminolevulinic acid and requires a succinyl CoA generati...

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Veröffentlicht in:	Analytical biochemistry 1972-06, Vol.47 (2), p.457-470
Hauptverfasser:	Strand, L.J., Swanson, A.L., Manning, J., Branch, S., Marver, H.S.
Format:	Artikel
Sprache:	eng
Schlagworte:	5-Aminolevulinate Synthetase - analysis 5-Aminolevulinate Synthetase - antagonists & inhibitors 5-Aminolevulinate Synthetase - blood Acyltransferases - analysis Animals Carbon Isotopes Chick Embryo Chromatography, Ion Exchange Coenzyme A Erythrocytes - enzymology Female Fibroblasts - enzymology Humans Levulinic Acids - isolation & purification Liver - embryology Liver - enzymology Methods Mice Microchemistry Rats Structure-Activity Relationship Succinates Tritium
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