Radiochemical microassay of δ-aminolevulinic acid synthetase in hepatic and erythroid tissues
A specific radiochemical microassay for δ-aminolevulinic acid synthetase is described. The assay is rapid and 10 to 500 times more sensitive than currently available methods. The assay measures the incorporation of radioactive succinate into δ-aminolevulinic acid and requires a succinyl CoA generati...
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| Veröffentlicht in: | Analytical biochemistry 1972-06, Vol.47 (2), p.457-470 |
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| container_end_page | 470 |
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| container_issue | 2 |
| container_start_page | 457 |
| container_title | Analytical biochemistry |
| container_volume | 47 |
| creator | Strand, L.J. Swanson, A.L. Manning, J. Branch, S. Marver, H.S. |
| description | A specific radiochemical microassay for δ-aminolevulinic acid synthetase is described. The assay is rapid and 10 to 500 times more sensitive than currently available methods. The assay measures the incorporation of radioactive succinate into δ-aminolevulinic acid and requires a succinyl CoA generating system; alternate pathways of succinate utilization are blocked by specific metabolic inhibitors. The δ-aminolevulinic acid formed is isolated by ion-exchange chromatography. The increased sensitivity of this assay has permitted the direct measurement of δ-aminolevulinic acid synthetase activity in cell cultures of avian hepatocytes, needle biopsy specimens of human liver, peripheral erythrocytes, and human fibroblasts. |
| doi_str_mv | 10.1016/0003-2697(72)90139-X |
| format | Article |
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The assay is rapid and 10 to 500 times more sensitive than currently available methods. The assay measures the incorporation of radioactive succinate into δ-aminolevulinic acid and requires a succinyl CoA generating system; alternate pathways of succinate utilization are blocked by specific metabolic inhibitors. The δ-aminolevulinic acid formed is isolated by ion-exchange chromatography. The increased sensitivity of this assay has permitted the direct measurement of δ-aminolevulinic acid synthetase activity in cell cultures of avian hepatocytes, needle biopsy specimens of human liver, peripheral erythrocytes, and human fibroblasts.</description><subject>5-Aminolevulinate Synthetase - analysis</subject><subject>5-Aminolevulinate Synthetase - antagonists & inhibitors</subject><subject>5-Aminolevulinate Synthetase - blood</subject><subject>Acyltransferases - analysis</subject><subject>Animals</subject><subject>Carbon Isotopes</subject><subject>Chick Embryo</subject><subject>Chromatography, Ion Exchange</subject><subject>Coenzyme A</subject><subject>Erythrocytes - enzymology</subject><subject>Female</subject><subject>Fibroblasts - enzymology</subject><subject>Humans</subject><subject>Levulinic Acids - isolation & purification</subject><subject>Liver - embryology</subject><subject>Liver - enzymology</subject><subject>Methods</subject><subject>Mice</subject><subject>Microchemistry</subject><subject>Rats</subject><subject>Structure-Activity Relationship</subject><subject>Succinates</subject><subject>Tritium</subject><issn>0003-2697</issn><issn>1096-0309</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1972</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kNtKxDAQhoMo63p4A4VeiV5UkyZNNzeCLJ5gQRCFvTKkyZRGeliTdqHv5XP4TKbuspdeZDLwf_8M8yN0RvA1wYTfYIxpnHCRXWbJlcCEini5h6YECx5jisU-mu6QQ3Tk_SfGhLCUT9AkxZSHboo-XpWxrS6htlpVUaiuVd6rIWqL6Oc7VrVt2grWfWUbqyOlrYn80HQldMpDZJuohJXqRqkxEbihK10bmM5634M_QQeFqjycbv9j9P5w_zZ_ihcvj8_zu0WsaZp1MeWC5oxqxpnipFAZAZbnBZllhglshDaJMYURhVFa5TollBqGE8JEzk1w0WN0sZm7cu1X2NvJ2noNVaUaaHsvZwFl4QWQbcBwp_cOCrlytlZukATLMVY5ZibHzGSWyL9Y5TLYzrfz-7wGszNtcwz67UaHcOTagpNeW2g0GOtAd9K09v8Fv66yieg</recordid><startdate>197206</startdate><enddate>197206</enddate><creator>Strand, L.J.</creator><creator>Swanson, A.L.</creator><creator>Manning, J.</creator><creator>Branch, S.</creator><creator>Marver, H.S.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>197206</creationdate><title>Radiochemical microassay of δ-aminolevulinic acid synthetase in hepatic and erythroid tissues</title><author>Strand, L.J. ; Swanson, A.L. ; Manning, J. ; Branch, S. ; Marver, H.S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c357t-3693b43c464a61fa71e4bbf187d490d9cd2ddfd9fdacabc5133d402149b6dc463</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1972</creationdate><topic>5-Aminolevulinate Synthetase - analysis</topic><topic>5-Aminolevulinate Synthetase - antagonists & inhibitors</topic><topic>5-Aminolevulinate Synthetase - blood</topic><topic>Acyltransferases - analysis</topic><topic>Animals</topic><topic>Carbon Isotopes</topic><topic>Chick Embryo</topic><topic>Chromatography, Ion Exchange</topic><topic>Coenzyme A</topic><topic>Erythrocytes - enzymology</topic><topic>Female</topic><topic>Fibroblasts - enzymology</topic><topic>Humans</topic><topic>Levulinic Acids - isolation & purification</topic><topic>Liver - embryology</topic><topic>Liver - enzymology</topic><topic>Methods</topic><topic>Mice</topic><topic>Microchemistry</topic><topic>Rats</topic><topic>Structure-Activity Relationship</topic><topic>Succinates</topic><topic>Tritium</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Strand, L.J.</creatorcontrib><creatorcontrib>Swanson, A.L.</creatorcontrib><creatorcontrib>Manning, J.</creatorcontrib><creatorcontrib>Branch, S.</creatorcontrib><creatorcontrib>Marver, H.S.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Strand, L.J.</au><au>Swanson, A.L.</au><au>Manning, J.</au><au>Branch, S.</au><au>Marver, H.S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Radiochemical microassay of δ-aminolevulinic acid synthetase in hepatic and erythroid tissues</atitle><jtitle>Analytical biochemistry</jtitle><addtitle>Anal Biochem</addtitle><date>1972-06</date><risdate>1972</risdate><volume>47</volume><issue>2</issue><spage>457</spage><epage>470</epage><pages>457-470</pages><issn>0003-2697</issn><eissn>1096-0309</eissn><abstract>A specific radiochemical microassay for δ-aminolevulinic acid synthetase is described. The assay is rapid and 10 to 500 times more sensitive than currently available methods. The assay measures the incorporation of radioactive succinate into δ-aminolevulinic acid and requires a succinyl CoA generating system; alternate pathways of succinate utilization are blocked by specific metabolic inhibitors. The δ-aminolevulinic acid formed is isolated by ion-exchange chromatography. The increased sensitivity of this assay has permitted the direct measurement of δ-aminolevulinic acid synthetase activity in cell cultures of avian hepatocytes, needle biopsy specimens of human liver, peripheral erythrocytes, and human fibroblasts.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>5036456</pmid><doi>10.1016/0003-2697(72)90139-X</doi><tpages>14</tpages></addata></record> |
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| ispartof | Analytical biochemistry, 1972-06, Vol.47 (2), p.457-470 |
| issn | 0003-2697 1096-0309 |
| language | eng |
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| subjects | 5-Aminolevulinate Synthetase - analysis 5-Aminolevulinate Synthetase - antagonists & inhibitors 5-Aminolevulinate Synthetase - blood Acyltransferases - analysis Animals Carbon Isotopes Chick Embryo Chromatography, Ion Exchange Coenzyme A Erythrocytes - enzymology Female Fibroblasts - enzymology Humans Levulinic Acids - isolation & purification Liver - embryology Liver - enzymology Methods Mice Microchemistry Rats Structure-Activity Relationship Succinates Tritium |
| title | Radiochemical microassay of δ-aminolevulinic acid synthetase in hepatic and erythroid tissues |
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