Purification and kinetic properties of galactokinase from human placenta

Galactokinase from human placenta was purified about 350-fold using DEAE-Sephadex-A-50 chromatography followed by Sephadex-G-200 and CM-Sephadex-C-50 filtration. The final steps of purification involved electrofocusing and ammonium sulfate precipitation. In analytical disc electrophoresis the purifi...

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Veröffentlicht in:	Archives of biochemistry and biophysics 1972-05, Vol.150 (1), p.191-198
Hauptverfasser:	Srivastava, Satish K., Blume, Karl-Georg, Van Loon, Cécile, Beutler, Ernest
Format:	Artikel
Sprache:	eng
Schlagworte:	Ammonium Sulfate Chemical Phenomena Chemical Precipitation Chemistry, Physical Chloromercuribenzoates Chromatography, Gel Chromatography, Ion Exchange Drug Stability Electrophoresis, Disc Enzyme Activation Erythrocytes - enzymology Ethylmaleimide Female Galactose Hexosephosphates Humans Isoelectric Focusing Kinetics Magnesium Molecular Weight Phosphotransferases - antagonists & inhibitors Phosphotransferases - isolation & purification Placenta - enzymology Pregnancy
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