Purification of 15-hydroxy prostaglandin dehydrogenase from bovine lung
15-Hydroxy prostaglandin dehydrogenase has been isolated in a highly purified state from bovine lung by a simple 3-step procedure. Spectrophotometric studies have shown that this enzyme catalyzes the conversion of PGE 2(11α,15(S)-dihydroxy-9-ketoprost-5,13-dienoic acid) into 15-keto-PGE 2((11α-hydro...
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| Veröffentlicht in: | Biochemical and biophysical research communications 1972-04, Vol.47 (1), p.96-102 |
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| container_title | Biochemical and biophysical research communications |
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| creator | Saeed, Sheikh A. Roy, Ashim C. |
| description | 15-Hydroxy prostaglandin dehydrogenase has been isolated in a highly purified state from bovine lung by a simple 3-step procedure. Spectrophotometric studies have shown that this enzyme catalyzes the conversion of PGE
2(11α,15(S)-dihydroxy-9-ketoprost-5,13-dienoic acid) into 15-keto-PGE
2((11α-hydroxy19,15-diketoprost-5,13-dienoic acid). |
| doi_str_mv | 10.1016/S0006-291X(72)80015-9 |
| format | Article |
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2(11α,15(S)-dihydroxy-9-ketoprost-5,13-dienoic acid) into 15-keto-PGE
2((11α-hydroxy19,15-diketoprost-5,13-dienoic acid).</description><subject>Acetone</subject><subject>Alcohol Oxidoreductases - isolation & purification</subject><subject>Ammonium Sulfate</subject><subject>animal science</subject><subject>Animals</subject><subject>Cattle</subject><subject>Chemical Precipitation</subject><subject>livestock</subject><subject>Lung - enzymology</subject><subject>NAD</subject><subject>Prostaglandins</subject><subject>zoology</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1972</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkM1KLDEQRoMoOld9BLFXoovWStKd7qxERL0XBAUV3IV0UhkjMx1NusV5ezM_uL2rgtSpqi-HkCMK5xSouHgCAFEySV9PG3bWAtC6lFtkQkFCyShU22Tyi-yRPym9Z4ZWQu6S3YrzpmJyQu4ex-idN3rwoS-CK_KWt4WN4XtRfMSQBj2d6d76vrC4ep9irxMWLoZ50YUv32MxG_vpAdlxepbwcFP3ycvtzfP13_L-4e7f9dV9aXhbDaVtJWpNpTWutlyyBtHxxiDTDoQxjauBAdXCCM67ppJdTa3UuqUoulqKlu-Tk_XeHO5zxDSouU8GZzkkhjGpllY1iGoJ1mvQ5F-kiE59RD_XcaEoqKVAtRKolnZUw9RKoJJ57mhzYOzmaH-nNsZy_3jddzooPY0-qZennJkDY20DwDJxuSYwe_jyGFUyHnuD1kc0g7LB_yfDDz1Uihk</recordid><startdate>19720414</startdate><enddate>19720414</enddate><creator>Saeed, Sheikh A.</creator><creator>Roy, Ashim C.</creator><general>Elsevier Inc</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19720414</creationdate><title>Purification of 15-hydroxy prostaglandin dehydrogenase from bovine lung</title><author>Saeed, Sheikh A. ; Roy, Ashim C.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c384t-d89eaa19dcf5d3927eef37ce2af06cc7f50201a6c633b749b51d9aa81e6b59683</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1972</creationdate><topic>Acetone</topic><topic>Alcohol Oxidoreductases - isolation & purification</topic><topic>Ammonium Sulfate</topic><topic>animal science</topic><topic>Animals</topic><topic>Cattle</topic><topic>Chemical Precipitation</topic><topic>livestock</topic><topic>Lung - enzymology</topic><topic>NAD</topic><topic>Prostaglandins</topic><topic>zoology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Saeed, Sheikh A.</creatorcontrib><creatorcontrib>Roy, Ashim C.</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Saeed, Sheikh A.</au><au>Roy, Ashim C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Purification of 15-hydroxy prostaglandin dehydrogenase from bovine lung</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>1972-04-14</date><risdate>1972</risdate><volume>47</volume><issue>1</issue><spage>96</spage><epage>102</epage><pages>96-102</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>15-Hydroxy prostaglandin dehydrogenase has been isolated in a highly purified state from bovine lung by a simple 3-step procedure. Spectrophotometric studies have shown that this enzyme catalyzes the conversion of PGE
2(11α,15(S)-dihydroxy-9-ketoprost-5,13-dienoic acid) into 15-keto-PGE
2((11α-hydroxy19,15-diketoprost-5,13-dienoic acid).</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>4337429</pmid><doi>10.1016/S0006-291X(72)80015-9</doi><tpages>7</tpages></addata></record> |
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| ispartof | Biochemical and biophysical research communications, 1972-04, Vol.47 (1), p.96-102 |
| issn | 0006-291X 1090-2104 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_81450648 |
| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Acetone Alcohol Oxidoreductases - isolation & purification Ammonium Sulfate animal science Animals Cattle Chemical Precipitation livestock Lung - enzymology NAD Prostaglandins zoology |
| title | Purification of 15-hydroxy prostaglandin dehydrogenase from bovine lung |
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