Determination of sedimentation coefficients and polyribosomal size in nonisokinetic density gradient centrifugation
An empirical function is derived relating the size of polyribosomes to their sedimentation coefficients. The function allows the calibration of centrifuged density gradients by using it in conjunction with the smaller polyribosomes as markers and relating centrifugations of different ∫ ω 2 dt throug...
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| Veröffentlicht in: | Analytical biochemistry 1972-04, Vol.46 (2), p.365-373 |
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| container_end_page | 373 |
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| container_title | Analytical biochemistry |
| container_volume | 46 |
| creator | Reisner, A.H. Askey, Carolyn Aylmer, Carolyn |
| description | An empirical function is derived relating the size of polyribosomes to their sedimentation coefficients. The function allows the calibration of centrifuged density gradients by using it in conjunction with the smaller polyribosomes as markers and relating centrifugations of different ∫
ω
2
dt through constants of proportionality. The method is such that stable gradients of any shape can be used and there is no need for precise knowledge of ∫
ω
2
dt. |
| doi_str_mv | 10.1016/0003-2697(72)90310-7 |
| format | Article |
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ω
2
dt through constants of proportionality. The method is such that stable gradients of any shape can be used and there is no need for precise knowledge of ∫
ω
2
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ω
2
dt through constants of proportionality. The method is such that stable gradients of any shape can be used and there is no need for precise knowledge of ∫
ω
2
dt.</description><subject>Animals</subject><subject>Centrifugation, Density Gradient</subject><subject>DNA</subject><subject>Liver</subject><subject>Mathematics</subject><subject>Methods</subject><subject>Paramecium - cytology</subject><subject>Proteins</subject><subject>Rats</subject><subject>Ribosomes</subject><subject>RNA, Ribosomal</subject><subject>Ultracentrifugation</subject><issn>0003-2697</issn><issn>1096-0309</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1972</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kEFr3DAQhUVpSbbb_oMGdCrpwclIsqXVpVCStgkEeknOwpZGQa0tbSVvYPvrI2eXHHuZgTfznjQfIZ8YXDBg8hIARMOlVueKf9EgGDTqDVkx0LIBAfotWb2unJL3pfwGYKzt5Ak56YC3kvEVKdc4Y55C7OeQIk2eFnRhwjgfBJvQ-2BDFQrto6PbNO5zGFJJUz_SEv4hDZHGFENJf0LEOVjqMJYw7-lj7t3ipLaWHPzu8SX0A3nn-7Hgx2Nfk4cf3--vbpq7Xz9vr77dNVZ0am700HVSA7duYPW7G-eZEO1GY9XFIKVQtQrpmO6HFpTQrWeSWQ-c68FtuFiTz4fcbU5_d1hmM4VicRz7iGlXzIa1rVIV0Zq0h0WbUykZvdnmMPV5bxiYhbVZQJoFpFHcvLA2qtrOjvm7YUL3ajrCrfOvhznWI58CZlMWkLYCzmhn41L4_wPPwyyPyg</recordid><startdate>197204</startdate><enddate>197204</enddate><creator>Reisner, A.H.</creator><creator>Askey, Carolyn</creator><creator>Aylmer, Carolyn</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>197204</creationdate><title>Determination of sedimentation coefficients and polyribosomal size in nonisokinetic density gradient centrifugation</title><author>Reisner, A.H. ; Askey, Carolyn ; Aylmer, Carolyn</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c357t-9b556902cdb10248df133489eb553b66373b636d19ab407394f161cf0229bd823</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1972</creationdate><topic>Animals</topic><topic>Centrifugation, Density Gradient</topic><topic>DNA</topic><topic>Liver</topic><topic>Mathematics</topic><topic>Methods</topic><topic>Paramecium - cytology</topic><topic>Proteins</topic><topic>Rats</topic><topic>Ribosomes</topic><topic>RNA, Ribosomal</topic><topic>Ultracentrifugation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Reisner, A.H.</creatorcontrib><creatorcontrib>Askey, Carolyn</creatorcontrib><creatorcontrib>Aylmer, Carolyn</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Reisner, A.H.</au><au>Askey, Carolyn</au><au>Aylmer, Carolyn</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Determination of sedimentation coefficients and polyribosomal size in nonisokinetic density gradient centrifugation</atitle><jtitle>Analytical biochemistry</jtitle><addtitle>Anal Biochem</addtitle><date>1972-04</date><risdate>1972</risdate><volume>46</volume><issue>2</issue><spage>365</spage><epage>373</epage><pages>365-373</pages><issn>0003-2697</issn><eissn>1096-0309</eissn><abstract>An empirical function is derived relating the size of polyribosomes to their sedimentation coefficients. The function allows the calibration of centrifuged density gradients by using it in conjunction with the smaller polyribosomes as markers and relating centrifugations of different ∫
ω
2
dt through constants of proportionality. The method is such that stable gradients of any shape can be used and there is no need for precise knowledge of ∫
ω
2
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| fulltext | fulltext |
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| ispartof | Analytical biochemistry, 1972-04, Vol.46 (2), p.365-373 |
| issn | 0003-2697 1096-0309 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_81447700 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Centrifugation, Density Gradient DNA Liver Mathematics Methods Paramecium - cytology Proteins Rats Ribosomes RNA, Ribosomal Ultracentrifugation |
| title | Determination of sedimentation coefficients and polyribosomal size in nonisokinetic density gradient centrifugation |
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