Swelling of hen erythrocyte nuclei in cytoplasm from Xenopus eggs
When a sperm enters a mature egg of Xenopus laevis both the sperm nucleus and egg pronucleus swell during the first 20 min. Erythrocyte and other nuclei also undergo a similar swelling when injected into Xenopus eggs. We have obtained a similar swelling of hen erythrocyte nuclei in cytoplasm isolate...
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| Veröffentlicht in: | Experimental cell research 1972-03, Vol.71 (1), p.90-96 |
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| container_title | Experimental cell research |
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| creator | Barry, J.M. Merriam, R.W. |
| description | When a sperm enters a mature egg of
Xenopus laevis both the sperm nucleus and egg pronucleus swell during the first 20 min. Erythrocyte and other nuclei also undergo a similar swelling when injected into
Xenopus eggs. We have obtained a similar swelling of hen erythrocyte nuclei in cytoplasm isolated from
Xenopus eggs. The swelling occurs in two stages. The first is an immediate small increase in volume similar to that which occurs in media with low concentrations of unbound cations and this can be prevented by adding magnesium ions to the cytoplasm. The second is a slower, irreversible increase to about 40 times the original volume. Neither of these types of swelling occurs in cytoplasm isolated from
Xenopus oocytes (as opposed to eggs) but the large irreversible increase in volume can be induced by the addition of heparin. In the light of these experiments we consider the physiological mechanism by which the sperm and egg pronucleus are induced to swell after penetration of the sperm. |
| doi_str_mv | 10.1016/0014-4827(72)90267-4 |
| format | Article |
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Xenopus laevis both the sperm nucleus and egg pronucleus swell during the first 20 min. Erythrocyte and other nuclei also undergo a similar swelling when injected into
Xenopus eggs. We have obtained a similar swelling of hen erythrocyte nuclei in cytoplasm isolated from
Xenopus eggs. The swelling occurs in two stages. The first is an immediate small increase in volume similar to that which occurs in media with low concentrations of unbound cations and this can be prevented by adding magnesium ions to the cytoplasm. The second is a slower, irreversible increase to about 40 times the original volume. Neither of these types of swelling occurs in cytoplasm isolated from
Xenopus oocytes (as opposed to eggs) but the large irreversible increase in volume can be induced by the addition of heparin. In the light of these experiments we consider the physiological mechanism by which the sperm and egg pronucleus are induced to swell after penetration of the sperm.</description><identifier>ISSN: 0014-4827</identifier><identifier>EISSN: 1090-2422</identifier><identifier>DOI: 10.1016/0014-4827(72)90267-4</identifier><identifier>PMID: 4554575</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Cell Fractionation ; Cell Nucleus - drug effects ; Cell Nucleus - metabolism ; Chickens ; Chlorides ; Chromatin ; Cytoplasm - drug effects ; Cytoplasm - metabolism ; Erythrocytes - cytology ; Female ; Fertilization ; Heparin - pharmacology ; Liver - cytology ; Magnesium ; Methods ; Microscopy, Phase-Contrast ; Ovum - cytology ; Ovum - metabolism ; Rats ; Time Factors ; Xenopus</subject><ispartof>Experimental cell research, 1972-03, Vol.71 (1), p.90-96</ispartof><rights>1972</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c357t-503d88dfb4e3d5e419889952c0ae20275fd604448066bb7d3c54d1bafb29f44f3</citedby><cites>FETCH-LOGICAL-c357t-503d88dfb4e3d5e419889952c0ae20275fd604448066bb7d3c54d1bafb29f44f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0014482772902674$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/4554575$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Barry, J.M.</creatorcontrib><creatorcontrib>Merriam, R.W.</creatorcontrib><title>Swelling of hen erythrocyte nuclei in cytoplasm from Xenopus eggs</title><title>Experimental cell research</title><addtitle>Exp Cell Res</addtitle><description>When a sperm enters a mature egg of
Xenopus laevis both the sperm nucleus and egg pronucleus swell during the first 20 min. Erythrocyte and other nuclei also undergo a similar swelling when injected into
Xenopus eggs. We have obtained a similar swelling of hen erythrocyte nuclei in cytoplasm isolated from
Xenopus eggs. The swelling occurs in two stages. The first is an immediate small increase in volume similar to that which occurs in media with low concentrations of unbound cations and this can be prevented by adding magnesium ions to the cytoplasm. The second is a slower, irreversible increase to about 40 times the original volume. Neither of these types of swelling occurs in cytoplasm isolated from
Xenopus oocytes (as opposed to eggs) but the large irreversible increase in volume can be induced by the addition of heparin. In the light of these experiments we consider the physiological mechanism by which the sperm and egg pronucleus are induced to swell after penetration of the sperm.</description><subject>Animals</subject><subject>Cell Fractionation</subject><subject>Cell Nucleus - drug effects</subject><subject>Cell Nucleus - metabolism</subject><subject>Chickens</subject><subject>Chlorides</subject><subject>Chromatin</subject><subject>Cytoplasm - drug effects</subject><subject>Cytoplasm - metabolism</subject><subject>Erythrocytes - cytology</subject><subject>Female</subject><subject>Fertilization</subject><subject>Heparin - pharmacology</subject><subject>Liver - cytology</subject><subject>Magnesium</subject><subject>Methods</subject><subject>Microscopy, Phase-Contrast</subject><subject>Ovum - cytology</subject><subject>Ovum - metabolism</subject><subject>Rats</subject><subject>Time Factors</subject><subject>Xenopus</subject><issn>0014-4827</issn><issn>1090-2422</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1972</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kEFr3DAQhUVI2WzS_oMGdArJwY0kjyz5UliWpC0EekgKvQlbGm1UbGsr2Q377-PNLjn2NAzz3puZj5DPnH3hjFe3jHEoQAt1rcRNzUSlCjghS85qVggQ4pQs3yVn5DznP4wxrXm1IAuQEqSSS7J6fMGuC8OGRk-fcaCYduNzinY3Ih0m22GgYaBzG7ddk3vqU-zpbxzidsoUN5v8kXzwTZfx07FekF_3d0_r78XDz28_1quHwpZSjYVkpdPa-RawdBKB11rXtRSWNSiYUNK7igGAZlXVtsqVVoLjbeNbUXsAX16Qq0PuNsW_E-bR9CHb-fhmwDhlozlApZSchXAQ2hRzTujNNoW-STvDmdmTM3ssZo_FKGHeyBmYbZfH_Knt0b2bjqjm-dfDHOcn_wVMJtuAg0UXEtrRuBj-v-AVX5l8mA</recordid><startdate>197203</startdate><enddate>197203</enddate><creator>Barry, J.M.</creator><creator>Merriam, R.W.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>197203</creationdate><title>Swelling of hen erythrocyte nuclei in cytoplasm from Xenopus eggs</title><author>Barry, J.M. ; Merriam, R.W.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c357t-503d88dfb4e3d5e419889952c0ae20275fd604448066bb7d3c54d1bafb29f44f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1972</creationdate><topic>Animals</topic><topic>Cell Fractionation</topic><topic>Cell Nucleus - drug effects</topic><topic>Cell Nucleus - metabolism</topic><topic>Chickens</topic><topic>Chlorides</topic><topic>Chromatin</topic><topic>Cytoplasm - drug effects</topic><topic>Cytoplasm - metabolism</topic><topic>Erythrocytes - cytology</topic><topic>Female</topic><topic>Fertilization</topic><topic>Heparin - pharmacology</topic><topic>Liver - cytology</topic><topic>Magnesium</topic><topic>Methods</topic><topic>Microscopy, Phase-Contrast</topic><topic>Ovum - cytology</topic><topic>Ovum - metabolism</topic><topic>Rats</topic><topic>Time Factors</topic><topic>Xenopus</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Barry, J.M.</creatorcontrib><creatorcontrib>Merriam, R.W.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Experimental cell research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Barry, J.M.</au><au>Merriam, R.W.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Swelling of hen erythrocyte nuclei in cytoplasm from Xenopus eggs</atitle><jtitle>Experimental cell research</jtitle><addtitle>Exp Cell Res</addtitle><date>1972-03</date><risdate>1972</risdate><volume>71</volume><issue>1</issue><spage>90</spage><epage>96</epage><pages>90-96</pages><issn>0014-4827</issn><eissn>1090-2422</eissn><abstract>When a sperm enters a mature egg of
Xenopus laevis both the sperm nucleus and egg pronucleus swell during the first 20 min. Erythrocyte and other nuclei also undergo a similar swelling when injected into
Xenopus eggs. We have obtained a similar swelling of hen erythrocyte nuclei in cytoplasm isolated from
Xenopus eggs. The swelling occurs in two stages. The first is an immediate small increase in volume similar to that which occurs in media with low concentrations of unbound cations and this can be prevented by adding magnesium ions to the cytoplasm. The second is a slower, irreversible increase to about 40 times the original volume. Neither of these types of swelling occurs in cytoplasm isolated from
Xenopus oocytes (as opposed to eggs) but the large irreversible increase in volume can be induced by the addition of heparin. In the light of these experiments we consider the physiological mechanism by which the sperm and egg pronucleus are induced to swell after penetration of the sperm.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>4554575</pmid><doi>10.1016/0014-4827(72)90267-4</doi><tpages>7</tpages></addata></record> |
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| ispartof | Experimental cell research, 1972-03, Vol.71 (1), p.90-96 |
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| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Cell Fractionation Cell Nucleus - drug effects Cell Nucleus - metabolism Chickens Chlorides Chromatin Cytoplasm - drug effects Cytoplasm - metabolism Erythrocytes - cytology Female Fertilization Heparin - pharmacology Liver - cytology Magnesium Methods Microscopy, Phase-Contrast Ovum - cytology Ovum - metabolism Rats Time Factors Xenopus |
| title | Swelling of hen erythrocyte nuclei in cytoplasm from Xenopus eggs |
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