Proteomic analysis of chronic restraint stress-induced Gan (肝)-stagnancy syndrome in rats

Objective To analyze the proteomic characteristics of Gan (肝)-stagnancy syndrome (GSS) by seeking the differential protein in blood and tissues of GSS model rats. Methods GSS model rats were established by chronic restraint stress, keeping rats in restrain chamber for 6 h every day for 21 successive...

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Veröffentlicht in:Chinese journal of integrative medicine 2010-12, Vol.16 (6), p.510-517
Hauptverfasser: Sun, Xue-gang, Zhong, Xiao-lan, Liu, Zhi-feng, Cai, Hong-bing, Fan, Qin, Wang, Qi-rui, Liu, Qiang, Song, Yu-hong, He, Song-qi, Zhang, Xu-fu, Lu, Zhi-ping
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container_issue 6
container_start_page 510
container_title Chinese journal of integrative medicine
container_volume 16
creator Sun, Xue-gang
Zhong, Xiao-lan
Liu, Zhi-feng
Cai, Hong-bing
Fan, Qin
Wang, Qi-rui
Liu, Qiang
Song, Yu-hong
He, Song-qi
Zhang, Xu-fu
Lu, Zhi-ping
description Objective To analyze the proteomic characteristics of Gan (肝)-stagnancy syndrome (GSS) by seeking the differential protein in blood and tissues of GSS model rats. Methods GSS model rats were established by chronic restraint stress, keeping rats in restrain chamber for 6 h every day for 21 successive days. Their blood and liver samples were collected at the end of experiment for differential protein detection with methods of isoelectrofocusing and polyacrylamide SDS-PAGE, silver staining, and scanning. The gel images were analyzed with Imagemaster 2D Elite software, and the excavated differential protein spots were identified with matrix assistant laser resolving TOF mass spectrometry, Western blot, ELISA, and RT-PCR, respectively. Results A method for isolating the protein in blood serum and tissues by two-dimensional gel electrophoresis was established and optimized. Six serum proteins and three liver proteins that differentially expressed were identified. The down-regulated differential proteins in serum of GSS model rats were serum albumin precursor, beta 1 globin, antibody against muscle acetylcholine receptor, Ig lambda-2 C region, and transthyretin (TTR), and those in liver tissue were aryl sulfotransferase, enoyl-CoA hydratase, and TTR. TTR down-regulation was found in both serum and liver. Preliminary biological information analysis showed that these differential proteins involved in immune, neuroendocrine, nutrition, and substance metabolism. Conclusion Proteomic analysis of differential proteins showed that TTR, aryl sulfotransferase, and enoyl-CoA hydratase expressions are downregulated in the GSS model rats, suggesting that the susceptibility of cancer could be enhanced by chronic stress.
doi_str_mv 10.1007/s11655-010-0525-z
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Methods GSS model rats were established by chronic restraint stress, keeping rats in restrain chamber for 6 h every day for 21 successive days. Their blood and liver samples were collected at the end of experiment for differential protein detection with methods of isoelectrofocusing and polyacrylamide SDS-PAGE, silver staining, and scanning. The gel images were analyzed with Imagemaster 2D Elite software, and the excavated differential protein spots were identified with matrix assistant laser resolving TOF mass spectrometry, Western blot, ELISA, and RT-PCR, respectively. Results A method for isolating the protein in blood serum and tissues by two-dimensional gel electrophoresis was established and optimized. Six serum proteins and three liver proteins that differentially expressed were identified. The down-regulated differential proteins in serum of GSS model rats were serum albumin precursor, beta 1 globin, antibody against muscle acetylcholine receptor, Ig lambda-2 C region, and transthyretin (TTR), and those in liver tissue were aryl sulfotransferase, enoyl-CoA hydratase, and TTR. TTR down-regulation was found in both serum and liver. Preliminary biological information analysis showed that these differential proteins involved in immune, neuroendocrine, nutrition, and substance metabolism. Conclusion Proteomic analysis of differential proteins showed that TTR, aryl sulfotransferase, and enoyl-CoA hydratase expressions are downregulated in the GSS model rats, suggesting that the susceptibility of cancer could be enhanced by chronic stress.</description><identifier>ISSN: 1672-0415</identifier><identifier>EISSN: 1993-0402</identifier><identifier>DOI: 10.1007/s11655-010-0525-z</identifier><identifier>PMID: 21110176</identifier><language>eng</language><publisher>Heidelberg: Chinese Association of Traditional and Western Medicine</publisher><subject>Amino Acid Sequence ; Animals ; Chronic Disease ; Disease Models, Animal ; Electrophoresis, Gel, Two-Dimensional ; Experimental Research ; Liver - metabolism ; Male ; Medicine ; Medicine &amp; Public Health ; Molecular Sequence Data ; Prealbumin - genetics ; Proteomics - methods ; Rats ; Rats, Wistar ; Reproducibility of Results ; Restraint, Physical ; Silver Staining ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization ; Stress, Psychological - complications ; Stress, Psychological - metabolism ; Syndrome ; Transcription, Genetic</subject><ispartof>Chinese journal of integrative medicine, 2010-12, Vol.16 (6), p.510-517</ispartof><rights>Chinese Association of the Integration of Traditional and Western Medicine and Springer-Verlag Berlin Heidelberg 2010</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c343t-cc00087e9cf7720b872621e1c1b16fc08b93c8ead94a211bdc56d0c76f09a2393</citedby><cites>FETCH-LOGICAL-c343t-cc00087e9cf7720b872621e1c1b16fc08b93c8ead94a211bdc56d0c76f09a2393</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11655-010-0525-z$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11655-010-0525-z$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21110176$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sun, Xue-gang</creatorcontrib><creatorcontrib>Zhong, Xiao-lan</creatorcontrib><creatorcontrib>Liu, Zhi-feng</creatorcontrib><creatorcontrib>Cai, Hong-bing</creatorcontrib><creatorcontrib>Fan, Qin</creatorcontrib><creatorcontrib>Wang, Qi-rui</creatorcontrib><creatorcontrib>Liu, Qiang</creatorcontrib><creatorcontrib>Song, Yu-hong</creatorcontrib><creatorcontrib>He, Song-qi</creatorcontrib><creatorcontrib>Zhang, Xu-fu</creatorcontrib><creatorcontrib>Lu, Zhi-ping</creatorcontrib><title>Proteomic analysis of chronic restraint stress-induced Gan (肝)-stagnancy syndrome in rats</title><title>Chinese journal of integrative medicine</title><addtitle>Chin. J. Integr. Med</addtitle><addtitle>Chin J Integr Med</addtitle><description>Objective To analyze the proteomic characteristics of Gan (肝)-stagnancy syndrome (GSS) by seeking the differential protein in blood and tissues of GSS model rats. Methods GSS model rats were established by chronic restraint stress, keeping rats in restrain chamber for 6 h every day for 21 successive days. Their blood and liver samples were collected at the end of experiment for differential protein detection with methods of isoelectrofocusing and polyacrylamide SDS-PAGE, silver staining, and scanning. The gel images were analyzed with Imagemaster 2D Elite software, and the excavated differential protein spots were identified with matrix assistant laser resolving TOF mass spectrometry, Western blot, ELISA, and RT-PCR, respectively. Results A method for isolating the protein in blood serum and tissues by two-dimensional gel electrophoresis was established and optimized. Six serum proteins and three liver proteins that differentially expressed were identified. The down-regulated differential proteins in serum of GSS model rats were serum albumin precursor, beta 1 globin, antibody against muscle acetylcholine receptor, Ig lambda-2 C region, and transthyretin (TTR), and those in liver tissue were aryl sulfotransferase, enoyl-CoA hydratase, and TTR. TTR down-regulation was found in both serum and liver. Preliminary biological information analysis showed that these differential proteins involved in immune, neuroendocrine, nutrition, and substance metabolism. Conclusion Proteomic analysis of differential proteins showed that TTR, aryl sulfotransferase, and enoyl-CoA hydratase expressions are downregulated in the GSS model rats, suggesting that the susceptibility of cancer could be enhanced by chronic stress.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Chronic Disease</subject><subject>Disease Models, Animal</subject><subject>Electrophoresis, Gel, Two-Dimensional</subject><subject>Experimental Research</subject><subject>Liver - metabolism</subject><subject>Male</subject><subject>Medicine</subject><subject>Medicine &amp; Public Health</subject><subject>Molecular Sequence Data</subject><subject>Prealbumin - genetics</subject><subject>Proteomics - methods</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Reproducibility of Results</subject><subject>Restraint, Physical</subject><subject>Silver Staining</subject><subject>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</subject><subject>Stress, Psychological - complications</subject><subject>Stress, Psychological - metabolism</subject><subject>Syndrome</subject><subject>Transcription, Genetic</subject><issn>1672-0415</issn><issn>1993-0402</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kM1O3TAQha0KVH7aB-im8o6yMMw4sZ0sqysKSEhlQVddWI7j0KAbm3qSxWXJe_B-PAZGF7rs6oxmzjkafYx9QThBAHNKiFopAQgClFTi4QPbx7atBNQgd8qsjSwzqj12QHQHoIwG9ZHtSUQENHqf_b7OaQ5pGj130a03NBJPA_d_copllwPN2Y1x5kUDkRhjv_jQ83MX-bfnx6djQbO7jS76DadN7HOaAh8jz26mT2x3cGsKn9_0kP36cXazuhBXP88vV9-vhK_qahbeA0BjQusHYyR0jZFaYkCPHerBQ9O1lW-C69valce73ivdgzd6gNbJqq0O2dG29z6nv0v52E4j-bBeuxjSQrbButbKKFOcuHX6nIhyGOx9HieXNxbBviK1W6S2ILWvSO1DyXx9a1-6KfT_Eu8Mi0FuDVRO8TZke5eWXGDSf1pfAGMbg0o</recordid><startdate>20101201</startdate><enddate>20101201</enddate><creator>Sun, Xue-gang</creator><creator>Zhong, Xiao-lan</creator><creator>Liu, Zhi-feng</creator><creator>Cai, Hong-bing</creator><creator>Fan, Qin</creator><creator>Wang, Qi-rui</creator><creator>Liu, Qiang</creator><creator>Song, Yu-hong</creator><creator>He, Song-qi</creator><creator>Zhang, Xu-fu</creator><creator>Lu, Zhi-ping</creator><general>Chinese Association of Traditional and Western Medicine</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20101201</creationdate><title>Proteomic analysis of chronic restraint stress-induced Gan (肝)-stagnancy syndrome in rats</title><author>Sun, Xue-gang ; Zhong, Xiao-lan ; Liu, Zhi-feng ; Cai, Hong-bing ; Fan, Qin ; Wang, Qi-rui ; Liu, Qiang ; Song, Yu-hong ; He, Song-qi ; Zhang, Xu-fu ; Lu, Zhi-ping</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c343t-cc00087e9cf7720b872621e1c1b16fc08b93c8ead94a211bdc56d0c76f09a2393</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Chronic Disease</topic><topic>Disease Models, Animal</topic><topic>Electrophoresis, Gel, Two-Dimensional</topic><topic>Experimental Research</topic><topic>Liver - metabolism</topic><topic>Male</topic><topic>Medicine</topic><topic>Medicine &amp; Public Health</topic><topic>Molecular Sequence Data</topic><topic>Prealbumin - genetics</topic><topic>Proteomics - methods</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Reproducibility of Results</topic><topic>Restraint, Physical</topic><topic>Silver Staining</topic><topic>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</topic><topic>Stress, Psychological - complications</topic><topic>Stress, Psychological - metabolism</topic><topic>Syndrome</topic><topic>Transcription, Genetic</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sun, Xue-gang</creatorcontrib><creatorcontrib>Zhong, Xiao-lan</creatorcontrib><creatorcontrib>Liu, Zhi-feng</creatorcontrib><creatorcontrib>Cai, Hong-bing</creatorcontrib><creatorcontrib>Fan, Qin</creatorcontrib><creatorcontrib>Wang, Qi-rui</creatorcontrib><creatorcontrib>Liu, Qiang</creatorcontrib><creatorcontrib>Song, Yu-hong</creatorcontrib><creatorcontrib>He, Song-qi</creatorcontrib><creatorcontrib>Zhang, Xu-fu</creatorcontrib><creatorcontrib>Lu, Zhi-ping</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Chinese journal of integrative medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sun, Xue-gang</au><au>Zhong, Xiao-lan</au><au>Liu, Zhi-feng</au><au>Cai, Hong-bing</au><au>Fan, Qin</au><au>Wang, Qi-rui</au><au>Liu, Qiang</au><au>Song, Yu-hong</au><au>He, Song-qi</au><au>Zhang, Xu-fu</au><au>Lu, Zhi-ping</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Proteomic analysis of chronic restraint stress-induced Gan (肝)-stagnancy syndrome in rats</atitle><jtitle>Chinese journal of integrative medicine</jtitle><stitle>Chin. J. Integr. Med</stitle><addtitle>Chin J Integr Med</addtitle><date>2010-12-01</date><risdate>2010</risdate><volume>16</volume><issue>6</issue><spage>510</spage><epage>517</epage><pages>510-517</pages><issn>1672-0415</issn><eissn>1993-0402</eissn><abstract>Objective To analyze the proteomic characteristics of Gan (肝)-stagnancy syndrome (GSS) by seeking the differential protein in blood and tissues of GSS model rats. Methods GSS model rats were established by chronic restraint stress, keeping rats in restrain chamber for 6 h every day for 21 successive days. Their blood and liver samples were collected at the end of experiment for differential protein detection with methods of isoelectrofocusing and polyacrylamide SDS-PAGE, silver staining, and scanning. The gel images were analyzed with Imagemaster 2D Elite software, and the excavated differential protein spots were identified with matrix assistant laser resolving TOF mass spectrometry, Western blot, ELISA, and RT-PCR, respectively. Results A method for isolating the protein in blood serum and tissues by two-dimensional gel electrophoresis was established and optimized. Six serum proteins and three liver proteins that differentially expressed were identified. The down-regulated differential proteins in serum of GSS model rats were serum albumin precursor, beta 1 globin, antibody against muscle acetylcholine receptor, Ig lambda-2 C region, and transthyretin (TTR), and those in liver tissue were aryl sulfotransferase, enoyl-CoA hydratase, and TTR. TTR down-regulation was found in both serum and liver. Preliminary biological information analysis showed that these differential proteins involved in immune, neuroendocrine, nutrition, and substance metabolism. Conclusion Proteomic analysis of differential proteins showed that TTR, aryl sulfotransferase, and enoyl-CoA hydratase expressions are downregulated in the GSS model rats, suggesting that the susceptibility of cancer could be enhanced by chronic stress.</abstract><cop>Heidelberg</cop><pub>Chinese Association of Traditional and Western Medicine</pub><pmid>21110176</pmid><doi>10.1007/s11655-010-0525-z</doi><tpages>8</tpages></addata></record>
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source MEDLINE; Alma/SFX Local Collection; SpringerLink Journals - AutoHoldings
subjects Amino Acid Sequence
Animals
Chronic Disease
Disease Models, Animal
Electrophoresis, Gel, Two-Dimensional
Experimental Research
Liver - metabolism
Male
Medicine
Medicine & Public Health
Molecular Sequence Data
Prealbumin - genetics
Proteomics - methods
Rats
Rats, Wistar
Reproducibility of Results
Restraint, Physical
Silver Staining
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Stress, Psychological - complications
Stress, Psychological - metabolism
Syndrome
Transcription, Genetic
title Proteomic analysis of chronic restraint stress-induced Gan (肝)-stagnancy syndrome in rats
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