Proteomic analysis of chronic restraint stress-induced Gan (肝)-stagnancy syndrome in rats
Objective To analyze the proteomic characteristics of Gan (肝)-stagnancy syndrome (GSS) by seeking the differential protein in blood and tissues of GSS model rats. Methods GSS model rats were established by chronic restraint stress, keeping rats in restrain chamber for 6 h every day for 21 successive...
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Veröffentlicht in: | Chinese journal of integrative medicine 2010-12, Vol.16 (6), p.510-517 |
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creator | Sun, Xue-gang Zhong, Xiao-lan Liu, Zhi-feng Cai, Hong-bing Fan, Qin Wang, Qi-rui Liu, Qiang Song, Yu-hong He, Song-qi Zhang, Xu-fu Lu, Zhi-ping |
description | Objective
To analyze the proteomic characteristics of Gan (肝)-stagnancy syndrome (GSS) by seeking the differential protein in blood and tissues of GSS model rats.
Methods
GSS model rats were established by chronic restraint stress, keeping rats in restrain chamber for 6 h every day for 21 successive days. Their blood and liver samples were collected at the end of experiment for differential protein detection with methods of isoelectrofocusing and polyacrylamide SDS-PAGE, silver staining, and scanning. The gel images were analyzed with Imagemaster 2D Elite software, and the excavated differential protein spots were identified with matrix assistant laser resolving TOF mass spectrometry, Western blot, ELISA, and RT-PCR, respectively.
Results
A method for isolating the protein in blood serum and tissues by two-dimensional gel electrophoresis was established and optimized. Six serum proteins and three liver proteins that differentially expressed were identified. The down-regulated differential proteins in serum of GSS model rats were serum albumin precursor, beta 1 globin, antibody against muscle acetylcholine receptor, Ig lambda-2 C region, and transthyretin (TTR), and those in liver tissue were aryl sulfotransferase, enoyl-CoA hydratase, and TTR. TTR down-regulation was found in both serum and liver. Preliminary biological information analysis showed that these differential proteins involved in immune, neuroendocrine, nutrition, and substance metabolism.
Conclusion
Proteomic analysis of differential proteins showed that TTR, aryl sulfotransferase, and enoyl-CoA hydratase expressions are downregulated in the GSS model rats, suggesting that the susceptibility of cancer could be enhanced by chronic stress. |
doi_str_mv | 10.1007/s11655-010-0525-z |
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To analyze the proteomic characteristics of Gan (肝)-stagnancy syndrome (GSS) by seeking the differential protein in blood and tissues of GSS model rats.
Methods
GSS model rats were established by chronic restraint stress, keeping rats in restrain chamber for 6 h every day for 21 successive days. Their blood and liver samples were collected at the end of experiment for differential protein detection with methods of isoelectrofocusing and polyacrylamide SDS-PAGE, silver staining, and scanning. The gel images were analyzed with Imagemaster 2D Elite software, and the excavated differential protein spots were identified with matrix assistant laser resolving TOF mass spectrometry, Western blot, ELISA, and RT-PCR, respectively.
Results
A method for isolating the protein in blood serum and tissues by two-dimensional gel electrophoresis was established and optimized. Six serum proteins and three liver proteins that differentially expressed were identified. The down-regulated differential proteins in serum of GSS model rats were serum albumin precursor, beta 1 globin, antibody against muscle acetylcholine receptor, Ig lambda-2 C region, and transthyretin (TTR), and those in liver tissue were aryl sulfotransferase, enoyl-CoA hydratase, and TTR. TTR down-regulation was found in both serum and liver. Preliminary biological information analysis showed that these differential proteins involved in immune, neuroendocrine, nutrition, and substance metabolism.
Conclusion
Proteomic analysis of differential proteins showed that TTR, aryl sulfotransferase, and enoyl-CoA hydratase expressions are downregulated in the GSS model rats, suggesting that the susceptibility of cancer could be enhanced by chronic stress.</description><identifier>ISSN: 1672-0415</identifier><identifier>EISSN: 1993-0402</identifier><identifier>DOI: 10.1007/s11655-010-0525-z</identifier><identifier>PMID: 21110176</identifier><language>eng</language><publisher>Heidelberg: Chinese Association of Traditional and Western Medicine</publisher><subject>Amino Acid Sequence ; Animals ; Chronic Disease ; Disease Models, Animal ; Electrophoresis, Gel, Two-Dimensional ; Experimental Research ; Liver - metabolism ; Male ; Medicine ; Medicine & Public Health ; Molecular Sequence Data ; Prealbumin - genetics ; Proteomics - methods ; Rats ; Rats, Wistar ; Reproducibility of Results ; Restraint, Physical ; Silver Staining ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization ; Stress, Psychological - complications ; Stress, Psychological - metabolism ; Syndrome ; Transcription, Genetic</subject><ispartof>Chinese journal of integrative medicine, 2010-12, Vol.16 (6), p.510-517</ispartof><rights>Chinese Association of the Integration of Traditional and Western Medicine and Springer-Verlag Berlin Heidelberg 2010</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c343t-cc00087e9cf7720b872621e1c1b16fc08b93c8ead94a211bdc56d0c76f09a2393</citedby><cites>FETCH-LOGICAL-c343t-cc00087e9cf7720b872621e1c1b16fc08b93c8ead94a211bdc56d0c76f09a2393</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11655-010-0525-z$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11655-010-0525-z$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21110176$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sun, Xue-gang</creatorcontrib><creatorcontrib>Zhong, Xiao-lan</creatorcontrib><creatorcontrib>Liu, Zhi-feng</creatorcontrib><creatorcontrib>Cai, Hong-bing</creatorcontrib><creatorcontrib>Fan, Qin</creatorcontrib><creatorcontrib>Wang, Qi-rui</creatorcontrib><creatorcontrib>Liu, Qiang</creatorcontrib><creatorcontrib>Song, Yu-hong</creatorcontrib><creatorcontrib>He, Song-qi</creatorcontrib><creatorcontrib>Zhang, Xu-fu</creatorcontrib><creatorcontrib>Lu, Zhi-ping</creatorcontrib><title>Proteomic analysis of chronic restraint stress-induced Gan (肝)-stagnancy syndrome in rats</title><title>Chinese journal of integrative medicine</title><addtitle>Chin. J. Integr. Med</addtitle><addtitle>Chin J Integr Med</addtitle><description>Objective
To analyze the proteomic characteristics of Gan (肝)-stagnancy syndrome (GSS) by seeking the differential protein in blood and tissues of GSS model rats.
Methods
GSS model rats were established by chronic restraint stress, keeping rats in restrain chamber for 6 h every day for 21 successive days. Their blood and liver samples were collected at the end of experiment for differential protein detection with methods of isoelectrofocusing and polyacrylamide SDS-PAGE, silver staining, and scanning. The gel images were analyzed with Imagemaster 2D Elite software, and the excavated differential protein spots were identified with matrix assistant laser resolving TOF mass spectrometry, Western blot, ELISA, and RT-PCR, respectively.
Results
A method for isolating the protein in blood serum and tissues by two-dimensional gel electrophoresis was established and optimized. Six serum proteins and three liver proteins that differentially expressed were identified. The down-regulated differential proteins in serum of GSS model rats were serum albumin precursor, beta 1 globin, antibody against muscle acetylcholine receptor, Ig lambda-2 C region, and transthyretin (TTR), and those in liver tissue were aryl sulfotransferase, enoyl-CoA hydratase, and TTR. TTR down-regulation was found in both serum and liver. Preliminary biological information analysis showed that these differential proteins involved in immune, neuroendocrine, nutrition, and substance metabolism.
Conclusion
Proteomic analysis of differential proteins showed that TTR, aryl sulfotransferase, and enoyl-CoA hydratase expressions are downregulated in the GSS model rats, suggesting that the susceptibility of cancer could be enhanced by chronic stress.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Chronic Disease</subject><subject>Disease Models, Animal</subject><subject>Electrophoresis, Gel, Two-Dimensional</subject><subject>Experimental Research</subject><subject>Liver - metabolism</subject><subject>Male</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Molecular Sequence Data</subject><subject>Prealbumin - genetics</subject><subject>Proteomics - methods</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Reproducibility of Results</subject><subject>Restraint, Physical</subject><subject>Silver Staining</subject><subject>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</subject><subject>Stress, Psychological - complications</subject><subject>Stress, Psychological - metabolism</subject><subject>Syndrome</subject><subject>Transcription, Genetic</subject><issn>1672-0415</issn><issn>1993-0402</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kM1O3TAQha0KVH7aB-im8o6yMMw4sZ0sqysKSEhlQVddWI7j0KAbm3qSxWXJe_B-PAZGF7rs6oxmzjkafYx9QThBAHNKiFopAQgClFTi4QPbx7atBNQgd8qsjSwzqj12QHQHoIwG9ZHtSUQENHqf_b7OaQ5pGj130a03NBJPA_d_copllwPN2Y1x5kUDkRhjv_jQ83MX-bfnx6djQbO7jS76DadN7HOaAh8jz26mT2x3cGsKn9_0kP36cXazuhBXP88vV9-vhK_qahbeA0BjQusHYyR0jZFaYkCPHerBQ9O1lW-C69valce73ivdgzd6gNbJqq0O2dG29z6nv0v52E4j-bBeuxjSQrbButbKKFOcuHX6nIhyGOx9HieXNxbBviK1W6S2ILWvSO1DyXx9a1-6KfT_Eu8Mi0FuDVRO8TZke5eWXGDSf1pfAGMbg0o</recordid><startdate>20101201</startdate><enddate>20101201</enddate><creator>Sun, Xue-gang</creator><creator>Zhong, Xiao-lan</creator><creator>Liu, Zhi-feng</creator><creator>Cai, Hong-bing</creator><creator>Fan, Qin</creator><creator>Wang, Qi-rui</creator><creator>Liu, Qiang</creator><creator>Song, Yu-hong</creator><creator>He, Song-qi</creator><creator>Zhang, Xu-fu</creator><creator>Lu, Zhi-ping</creator><general>Chinese Association of Traditional and Western Medicine</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20101201</creationdate><title>Proteomic analysis of chronic restraint stress-induced Gan (肝)-stagnancy syndrome in rats</title><author>Sun, Xue-gang ; Zhong, Xiao-lan ; Liu, Zhi-feng ; Cai, Hong-bing ; Fan, Qin ; Wang, Qi-rui ; Liu, Qiang ; Song, Yu-hong ; He, Song-qi ; Zhang, Xu-fu ; Lu, Zhi-ping</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c343t-cc00087e9cf7720b872621e1c1b16fc08b93c8ead94a211bdc56d0c76f09a2393</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Chronic Disease</topic><topic>Disease Models, Animal</topic><topic>Electrophoresis, Gel, Two-Dimensional</topic><topic>Experimental Research</topic><topic>Liver - metabolism</topic><topic>Male</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Molecular Sequence Data</topic><topic>Prealbumin - genetics</topic><topic>Proteomics - methods</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Reproducibility of Results</topic><topic>Restraint, Physical</topic><topic>Silver Staining</topic><topic>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</topic><topic>Stress, Psychological - complications</topic><topic>Stress, Psychological - metabolism</topic><topic>Syndrome</topic><topic>Transcription, Genetic</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sun, Xue-gang</creatorcontrib><creatorcontrib>Zhong, Xiao-lan</creatorcontrib><creatorcontrib>Liu, Zhi-feng</creatorcontrib><creatorcontrib>Cai, Hong-bing</creatorcontrib><creatorcontrib>Fan, Qin</creatorcontrib><creatorcontrib>Wang, Qi-rui</creatorcontrib><creatorcontrib>Liu, Qiang</creatorcontrib><creatorcontrib>Song, Yu-hong</creatorcontrib><creatorcontrib>He, Song-qi</creatorcontrib><creatorcontrib>Zhang, Xu-fu</creatorcontrib><creatorcontrib>Lu, Zhi-ping</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Chinese journal of integrative medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sun, Xue-gang</au><au>Zhong, Xiao-lan</au><au>Liu, Zhi-feng</au><au>Cai, Hong-bing</au><au>Fan, Qin</au><au>Wang, Qi-rui</au><au>Liu, Qiang</au><au>Song, Yu-hong</au><au>He, Song-qi</au><au>Zhang, Xu-fu</au><au>Lu, Zhi-ping</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Proteomic analysis of chronic restraint stress-induced Gan (肝)-stagnancy syndrome in rats</atitle><jtitle>Chinese journal of integrative medicine</jtitle><stitle>Chin. J. Integr. Med</stitle><addtitle>Chin J Integr Med</addtitle><date>2010-12-01</date><risdate>2010</risdate><volume>16</volume><issue>6</issue><spage>510</spage><epage>517</epage><pages>510-517</pages><issn>1672-0415</issn><eissn>1993-0402</eissn><abstract>Objective
To analyze the proteomic characteristics of Gan (肝)-stagnancy syndrome (GSS) by seeking the differential protein in blood and tissues of GSS model rats.
Methods
GSS model rats were established by chronic restraint stress, keeping rats in restrain chamber for 6 h every day for 21 successive days. Their blood and liver samples were collected at the end of experiment for differential protein detection with methods of isoelectrofocusing and polyacrylamide SDS-PAGE, silver staining, and scanning. The gel images were analyzed with Imagemaster 2D Elite software, and the excavated differential protein spots were identified with matrix assistant laser resolving TOF mass spectrometry, Western blot, ELISA, and RT-PCR, respectively.
Results
A method for isolating the protein in blood serum and tissues by two-dimensional gel electrophoresis was established and optimized. Six serum proteins and three liver proteins that differentially expressed were identified. The down-regulated differential proteins in serum of GSS model rats were serum albumin precursor, beta 1 globin, antibody against muscle acetylcholine receptor, Ig lambda-2 C region, and transthyretin (TTR), and those in liver tissue were aryl sulfotransferase, enoyl-CoA hydratase, and TTR. TTR down-regulation was found in both serum and liver. Preliminary biological information analysis showed that these differential proteins involved in immune, neuroendocrine, nutrition, and substance metabolism.
Conclusion
Proteomic analysis of differential proteins showed that TTR, aryl sulfotransferase, and enoyl-CoA hydratase expressions are downregulated in the GSS model rats, suggesting that the susceptibility of cancer could be enhanced by chronic stress.</abstract><cop>Heidelberg</cop><pub>Chinese Association of Traditional and Western Medicine</pub><pmid>21110176</pmid><doi>10.1007/s11655-010-0525-z</doi><tpages>8</tpages></addata></record> |
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subjects | Amino Acid Sequence Animals Chronic Disease Disease Models, Animal Electrophoresis, Gel, Two-Dimensional Experimental Research Liver - metabolism Male Medicine Medicine & Public Health Molecular Sequence Data Prealbumin - genetics Proteomics - methods Rats Rats, Wistar Reproducibility of Results Restraint, Physical Silver Staining Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Stress, Psychological - complications Stress, Psychological - metabolism Syndrome Transcription, Genetic |
title | Proteomic analysis of chronic restraint stress-induced Gan (肝)-stagnancy syndrome in rats |
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