Development of retinol-binding protein 4 immunocolloidal gold fast test strip using high-sensitivity monoclonal antibodies generated by DNA immunization
DNA immunization is an efficient method for high-affinity monoclonal antibody generation. Here, we describe the generation of several high-quality monoclonal antibodies （mAbs） against retinol-binding protein 4 （RBP4）, an important marker for kidney abnormality and dysfunction, with a combination met...
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| Veröffentlicht in: | Acta biochimica et biophysica Sinica 2010-12, Vol.42 (12), p.847-853 |
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| container_title | Acta biochimica et biophysica Sinica |
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| creator | Bian, Chao Zhang, Fang Wang, Feng Ling, Zhiyang Luo, Min Wu, Hongqiang Sun, Yizhuo Li, Junhui Li, Bingnan Zhu, Jingyan Tang, Linna Zhou, Yanyan Shi, Qunfang Ji, Yongyong Tian, Lin Lin, Guomei Fan, Yu Wang, Niansong Sun, Bing |
| description | DNA immunization is an efficient method for high-affinity monoclonal antibody generation. Here, we describe the generation of several high-quality monoclonal antibodies （mAbs） against retinol-binding protein 4 （RBP4）, an important marker for kidney abnormality and dysfunction, with a combination method of DNA priming and protein boost. The mAbs generated could bind to RBP4 with high sensitivity and using these mAbs, an immunocoHoidal gold fast test strip was constructed. The strip can give a result in 〈5 rain and is very sensitive with a detection limit of about 1 ng/ml. A small-scale clinical test revealed that the result of this strip was well in accordance with that of an enzymelabeled immunosorbent assay kit currently available on the market. Consequently, it could be useful for more convenient and faster RBP4 determination in the clinic. |
| doi_str_mv | 10.1093/abbs/gmq099 |
| format | Article |
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Here, we describe the generation of several high-quality monoclonal antibodies （mAbs） against retinol-binding protein 4 （RBP4）, an important marker for kidney abnormality and dysfunction, with a combination method of DNA priming and protein boost. The mAbs generated could bind to RBP4 with high sensitivity and using these mAbs, an immunocoHoidal gold fast test strip was constructed. The strip can give a result in 〈5 rain and is very sensitive with a detection limit of about 1 ng/ml. A small-scale clinical test revealed that the result of this strip was well in accordance with that of an enzymelabeled immunosorbent assay kit currently available on the market. Consequently, it could be useful for more convenient and faster RBP4 determination in the clinic.</description><identifier>ISSN: 1672-9145</identifier><identifier>EISSN: 1745-7270</identifier><identifier>DOI: 10.1093/abbs/gmq099</identifier><identifier>PMID: 21062789</identifier><language>eng</language><publisher>China: Oxford University Press</publisher><subject>Antibodies, Monoclonal - immunology ; Base Sequence ; DNA - genetics ; DNA - immunology ; DNA免疫 ; Enzyme-Linked Immunosorbent Assay - methods ; Gold Colloid ; Humans ; Immunization - methods ; Kidney Diseases - genetics ; Kidney Diseases - pathology ; Reagent Kits, Diagnostic ; Reagent Strips ; Retinol-Binding Proteins, Plasma - genetics ; Retinol-Binding Proteins, Plasma - immunology ; Sensitivity and Specificity ; 临床试验 ; 免疫检测试剂盒 ; 单克隆抗体 ; 快速检测试纸 ; 视黄醇结合蛋白 ; 高灵敏度</subject><ispartof>Acta biochimica et biophysica Sinica, 2010-12, Vol.42 (12), p.847-853</ispartof><rights>The Author 2010. Published by ABBS Editorial Office in association with Oxford University Press on behalf of the Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences. 2010</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c346t-2e757f4ea2321016d74a8174ea996e61dfe17eeb29c47e76937503121c3754b03</citedby><cites>FETCH-LOGICAL-c346t-2e757f4ea2321016d74a8174ea996e61dfe17eeb29c47e76937503121c3754b03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://image.cqvip.com/vip1000/qk/90160X/90160X.jpg</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21062789$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bian, Chao</creatorcontrib><creatorcontrib>Zhang, Fang</creatorcontrib><creatorcontrib>Wang, Feng</creatorcontrib><creatorcontrib>Ling, Zhiyang</creatorcontrib><creatorcontrib>Luo, Min</creatorcontrib><creatorcontrib>Wu, Hongqiang</creatorcontrib><creatorcontrib>Sun, Yizhuo</creatorcontrib><creatorcontrib>Li, Junhui</creatorcontrib><creatorcontrib>Li, Bingnan</creatorcontrib><creatorcontrib>Zhu, Jingyan</creatorcontrib><creatorcontrib>Tang, Linna</creatorcontrib><creatorcontrib>Zhou, Yanyan</creatorcontrib><creatorcontrib>Shi, Qunfang</creatorcontrib><creatorcontrib>Ji, Yongyong</creatorcontrib><creatorcontrib>Tian, Lin</creatorcontrib><creatorcontrib>Lin, Guomei</creatorcontrib><creatorcontrib>Fan, Yu</creatorcontrib><creatorcontrib>Wang, Niansong</creatorcontrib><creatorcontrib>Sun, Bing</creatorcontrib><title>Development of retinol-binding protein 4 immunocolloidal gold fast test strip using high-sensitivity monoclonal antibodies generated by DNA immunization</title><title>Acta biochimica et biophysica Sinica</title><addtitle>Acta Biochimica et Biophysica Sinica</addtitle><description>DNA immunization is an efficient method for high-affinity monoclonal antibody generation. Here, we describe the generation of several high-quality monoclonal antibodies （mAbs） against retinol-binding protein 4 （RBP4）, an important marker for kidney abnormality and dysfunction, with a combination method of DNA priming and protein boost. The mAbs generated could bind to RBP4 with high sensitivity and using these mAbs, an immunocoHoidal gold fast test strip was constructed. The strip can give a result in 〈5 rain and is very sensitive with a detection limit of about 1 ng/ml. A small-scale clinical test revealed that the result of this strip was well in accordance with that of an enzymelabeled immunosorbent assay kit currently available on the market. Consequently, it could be useful for more convenient and faster RBP4 determination in the clinic.</description><subject>Antibodies, Monoclonal - immunology</subject><subject>Base Sequence</subject><subject>DNA - genetics</subject><subject>DNA - immunology</subject><subject>DNA免疫</subject><subject>Enzyme-Linked Immunosorbent Assay - methods</subject><subject>Gold Colloid</subject><subject>Humans</subject><subject>Immunization - methods</subject><subject>Kidney Diseases - genetics</subject><subject>Kidney Diseases - pathology</subject><subject>Reagent Kits, Diagnostic</subject><subject>Reagent Strips</subject><subject>Retinol-Binding Proteins, Plasma - genetics</subject><subject>Retinol-Binding Proteins, Plasma - immunology</subject><subject>Sensitivity and Specificity</subject><subject>临床试验</subject><subject>免疫检测试剂盒</subject><subject>单克隆抗体</subject><subject>快速检测试纸</subject><subject>视黄醇结合蛋白</subject><subject>高灵敏度</subject><issn>1672-9145</issn><issn>1745-7270</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUtv1DAUhSNERR-wYo8sFu0ChfoVe7ysWh6VqrKBtWUnNxmDY2dip9LwS_i5eJSBbTf2XXznXN1zquotwR8JVuzaWJuuh3GHlXpRnRHJm1pSiV-WWUhaK8Kb0-o8pZ8YMyEIflWdUoIFlRt1Vv25gyfwcRohZBR7NEN2IfrautC5MKBpjhlcQBy5cVxCbKP30XXGoyH6DvUmZZShPCnPbkJLOoi2btjWCUJy2T25vEdjLEofQ5GZkJ2NnYOEBggwmwwdsnt093izrnC_TXYxvK5OeuMTvDn-F9WPz5--336tH759ub-9eahbxkWuKchG9hwMZeUoIjrJzaZkAEYpAYJ0PRAJYKlquQQpFJMNZoSStgzcYnZRXa2-5dLdUi7Ro0steG8CxCXpDeFccEVJIT-sZDvHlGbo9TS70cx7TbA-NKEPTei1iUK_O_oudoTuP_sv-gJcrkBcpmec3h_3bmMYdiVgbU37q3ceNBMMNxwL9hfJgKEd</recordid><startdate>20101201</startdate><enddate>20101201</enddate><creator>Bian, Chao</creator><creator>Zhang, Fang</creator><creator>Wang, Feng</creator><creator>Ling, Zhiyang</creator><creator>Luo, Min</creator><creator>Wu, Hongqiang</creator><creator>Sun, Yizhuo</creator><creator>Li, Junhui</creator><creator>Li, Bingnan</creator><creator>Zhu, Jingyan</creator><creator>Tang, Linna</creator><creator>Zhou, Yanyan</creator><creator>Shi, Qunfang</creator><creator>Ji, Yongyong</creator><creator>Tian, Lin</creator><creator>Lin, Guomei</creator><creator>Fan, Yu</creator><creator>Wang, Niansong</creator><creator>Sun, Bing</creator><general>Oxford University Press</general><scope>2RA</scope><scope>92L</scope><scope>CQIGP</scope><scope>W95</scope><scope>~WA</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20101201</creationdate><title>Development of retinol-binding protein 4 immunocolloidal gold fast test strip using high-sensitivity monoclonal antibodies generated by DNA immunization</title><author>Bian, Chao ; Zhang, Fang ; Wang, Feng ; Ling, Zhiyang ; Luo, Min ; Wu, Hongqiang ; Sun, Yizhuo ; Li, Junhui ; Li, Bingnan ; Zhu, Jingyan ; Tang, Linna ; Zhou, Yanyan ; Shi, Qunfang ; Ji, Yongyong ; Tian, Lin ; Lin, Guomei ; Fan, Yu ; Wang, Niansong ; Sun, Bing</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c346t-2e757f4ea2321016d74a8174ea996e61dfe17eeb29c47e76937503121c3754b03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Antibodies, Monoclonal - immunology</topic><topic>Base Sequence</topic><topic>DNA - genetics</topic><topic>DNA - immunology</topic><topic>DNA免疫</topic><topic>Enzyme-Linked Immunosorbent Assay - methods</topic><topic>Gold Colloid</topic><topic>Humans</topic><topic>Immunization - methods</topic><topic>Kidney Diseases - genetics</topic><topic>Kidney Diseases - pathology</topic><topic>Reagent Kits, Diagnostic</topic><topic>Reagent Strips</topic><topic>Retinol-Binding Proteins, Plasma - genetics</topic><topic>Retinol-Binding Proteins, Plasma - immunology</topic><topic>Sensitivity and Specificity</topic><topic>临床试验</topic><topic>免疫检测试剂盒</topic><topic>单克隆抗体</topic><topic>快速检测试纸</topic><topic>视黄醇结合蛋白</topic><topic>高灵敏度</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bian, Chao</creatorcontrib><creatorcontrib>Zhang, Fang</creatorcontrib><creatorcontrib>Wang, Feng</creatorcontrib><creatorcontrib>Ling, Zhiyang</creatorcontrib><creatorcontrib>Luo, Min</creatorcontrib><creatorcontrib>Wu, Hongqiang</creatorcontrib><creatorcontrib>Sun, Yizhuo</creatorcontrib><creatorcontrib>Li, Junhui</creatorcontrib><creatorcontrib>Li, Bingnan</creatorcontrib><creatorcontrib>Zhu, Jingyan</creatorcontrib><creatorcontrib>Tang, Linna</creatorcontrib><creatorcontrib>Zhou, Yanyan</creatorcontrib><creatorcontrib>Shi, Qunfang</creatorcontrib><creatorcontrib>Ji, Yongyong</creatorcontrib><creatorcontrib>Tian, Lin</creatorcontrib><creatorcontrib>Lin, Guomei</creatorcontrib><creatorcontrib>Fan, Yu</creatorcontrib><creatorcontrib>Wang, Niansong</creatorcontrib><creatorcontrib>Sun, Bing</creatorcontrib><collection>中文科技期刊数据库</collection><collection>中文科技期刊数据库-CALIS站点</collection><collection>中文科技期刊数据库-7.0平台</collection><collection>中文科技期刊数据库-农业科学</collection><collection>中文科技期刊数据库- 镜像站点</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Acta biochimica et biophysica Sinica</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bian, Chao</au><au>Zhang, Fang</au><au>Wang, Feng</au><au>Ling, Zhiyang</au><au>Luo, Min</au><au>Wu, Hongqiang</au><au>Sun, Yizhuo</au><au>Li, Junhui</au><au>Li, Bingnan</au><au>Zhu, Jingyan</au><au>Tang, Linna</au><au>Zhou, Yanyan</au><au>Shi, Qunfang</au><au>Ji, Yongyong</au><au>Tian, Lin</au><au>Lin, Guomei</au><au>Fan, Yu</au><au>Wang, Niansong</au><au>Sun, Bing</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Development of retinol-binding protein 4 immunocolloidal gold fast test strip using high-sensitivity monoclonal antibodies generated by DNA immunization</atitle><jtitle>Acta biochimica et biophysica Sinica</jtitle><addtitle>Acta Biochimica et Biophysica Sinica</addtitle><date>2010-12-01</date><risdate>2010</risdate><volume>42</volume><issue>12</issue><spage>847</spage><epage>853</epage><pages>847-853</pages><issn>1672-9145</issn><eissn>1745-7270</eissn><abstract>DNA immunization is an efficient method for high-affinity monoclonal antibody generation. Here, we describe the generation of several high-quality monoclonal antibodies （mAbs） against retinol-binding protein 4 （RBP4）, an important marker for kidney abnormality and dysfunction, with a combination method of DNA priming and protein boost. The mAbs generated could bind to RBP4 with high sensitivity and using these mAbs, an immunocoHoidal gold fast test strip was constructed. The strip can give a result in 〈5 rain and is very sensitive with a detection limit of about 1 ng/ml. A small-scale clinical test revealed that the result of this strip was well in accordance with that of an enzymelabeled immunosorbent assay kit currently available on the market. Consequently, it could be useful for more convenient and faster RBP4 determination in the clinic.</abstract><cop>China</cop><pub>Oxford University Press</pub><pmid>21062789</pmid><doi>10.1093/abbs/gmq099</doi><tpages>7</tpages></addata></record> |
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| subjects | Antibodies, Monoclonal - immunology Base Sequence DNA - genetics DNA - immunology DNA免疫 Enzyme-Linked Immunosorbent Assay - methods Gold Colloid Humans Immunization - methods Kidney Diseases - genetics Kidney Diseases - pathology Reagent Kits, Diagnostic Reagent Strips Retinol-Binding Proteins, Plasma - genetics Retinol-Binding Proteins, Plasma - immunology Sensitivity and Specificity 临床试验 免疫检测试剂盒 单克隆抗体 快速检测试纸 视黄醇结合蛋白 高灵敏度 |
| title | Development of retinol-binding protein 4 immunocolloidal gold fast test strip using high-sensitivity monoclonal antibodies generated by DNA immunization |
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