Insulin, Leptin, and Tumoral Adipocytes Promote Murine Pancreatic Cancer Growth
Background Obesity accelerates development and growth of human pancreatic cancer. We recently reported similar findings in a novel murine model of pancreatic cancer in congenitally obese mice. The current experiments were designed to evaluate the effects of diet-induced obesity on pancreatic cancer...
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| Veröffentlicht in: | Journal of gastrointestinal surgery 2010-12, Vol.14 (12), p.1888-1894 |
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| creator | White, Patrick B. True, Eben M. Ziegler, Kathryn M. Wang, Sue S. Swartz-Basile, Deborah A. Pitt, Henry A. Zyromski, Nicholas J. |
| description | Background
Obesity accelerates development and growth of human pancreatic cancer. We recently reported similar findings in a novel murine model of pancreatic cancer in congenitally obese mice. The current experiments were designed to evaluate the effects of diet-induced obesity on pancreatic cancer growth.
Methods
Thirty C57BL/6J female mice were fed either control 10% fat (
n
= 10) or 60% fat diet (
n
= 20) starting at age 6 weeks. At 11 weeks, 2.5 × 10
5
PAN02 murine pancreatic cancer cells were inoculated. After 6 weeks, tumors were harvested. Serum adiponectin, leptin, insulin, and glucose concentrations were measured. Tumor proliferation, apoptosis, adipocyte content, and tumor-infiltrating lymphocytes were evaluated.
Results
The diet-induced obesity diet led to significant weight gain (control 21.3 ± 0.6 g; diet-induced obesity 23.1 ± 0.5 g;
p
= 0.03). Mice heavier than 23.1 g were considered “Overweight.” Tumors grew significantly larger in overweight (1.3 ± 0.3 g) compared to lean (0.5 ± 0.2 g;
p
= 0.03) mice; tumor size correlated positively with body weight (
R
= 0.56;
p
|
| doi_str_mv | 10.1007/s11605-010-1349-x |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_814462732</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>814462732</sourcerecordid><originalsourceid>FETCH-LOGICAL-c437t-ef48b666da6eb2dc84b967fd8733ecda1a67dfa8fff6174142a92805cc5bf0423</originalsourceid><addsrcrecordid>eNp1kE1LxDAQhoMofv8AL1Lw4MXqTJom7VEWXYWV3YOCt5CmiVbaZk1a1H9vllURwdNMyDPvDA8hRwjnCCAuAiKHPAWEFDNWpu8bZBcLkaWMU74ZeygxpXn-uEP2QngBQAFYbJMdCkVeCoBdMr_tw9g2_VkyM8thVVVfJ_dj57xqk8u6WTr9MZiQLLzr3GCSu9E3vUkWqtfeqKHRySS2xidT796G5wOyZVUbzOFX3ScP11f3k5t0Np_eTi5nqWaZGFJjWVFxzmvFTUVrXbCq5MLW8fjM6Fqh4qK2qrDWchQMGVUlLSDXOq8sMJrtk9N17tK719GEQXZN0KZtVW_cGGSBLEoQ2Yo8-UO-uNH38TiJiDTjUGYYKVxT2rsQvLFy6ZtO-Q-JIFey5Vq2hNU7ypbvceb4K3msOlP_THzbjQBdAyF-9U_G_1r9b-onJuOJ_A</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1112360931</pqid></control><display><type>article</type><title>Insulin, Leptin, and Tumoral Adipocytes Promote Murine Pancreatic Cancer Growth</title><source>MEDLINE</source><source>SpringerLink Journals - AutoHoldings</source><creator>White, Patrick B. ; True, Eben M. ; Ziegler, Kathryn M. ; Wang, Sue S. ; Swartz-Basile, Deborah A. ; Pitt, Henry A. ; Zyromski, Nicholas J.</creator><creatorcontrib>White, Patrick B. ; True, Eben M. ; Ziegler, Kathryn M. ; Wang, Sue S. ; Swartz-Basile, Deborah A. ; Pitt, Henry A. ; Zyromski, Nicholas J.</creatorcontrib><description>Background
Obesity accelerates development and growth of human pancreatic cancer. We recently reported similar findings in a novel murine model of pancreatic cancer in congenitally obese mice. The current experiments were designed to evaluate the effects of diet-induced obesity on pancreatic cancer growth.
Methods
Thirty C57BL/6J female mice were fed either control 10% fat (
n
= 10) or 60% fat diet (
n
= 20) starting at age 6 weeks. At 11 weeks, 2.5 × 10
5
PAN02 murine pancreatic cancer cells were inoculated. After 6 weeks, tumors were harvested. Serum adiponectin, leptin, insulin, and glucose concentrations were measured. Tumor proliferation, apoptosis, adipocyte content, and tumor-infiltrating lymphocytes were evaluated.
Results
The diet-induced obesity diet led to significant weight gain (control 21.3 ± 0.6 g; diet-induced obesity 23.1 ± 0.5 g;
p
= 0.03). Mice heavier than 23.1 g were considered “Overweight.” Tumors grew significantly larger in overweight (1.3 ± 0.3 g) compared to lean (0.5 ± 0.2 g;
p
= 0.03) mice; tumor size correlated positively with body weight (
R
= 0.56;
p
< 0.02). Serum leptin (3.1 ± 0.7 vs. 1.4 ± 0.2 ng/ml) and insulin (0.5 ± 0.2 vs. 0.18 ± 0.02 ng/ml) were significantly greater in overweight mice. Tumor proliferation, apoptosis, and tumor adipocyte volume were similar. T and B lymphocytes were observed infiltrating tumors from lean and overweight mice in similar number.
Conclusion
These data show that diet-induced obesity accelerates the growth of murine pancreatic cancer.</description><identifier>ISSN: 1091-255X</identifier><identifier>EISSN: 1873-4626</identifier><identifier>DOI: 10.1007/s11605-010-1349-x</identifier><identifier>PMID: 20859700</identifier><language>eng</language><publisher>New York: Springer-Verlag</publisher><subject>2010 SSAT Plenary Presentation ; Adipocytes - physiology ; Animals ; Cell Proliferation ; Female ; Gastroenterology ; Insulin - physiology ; Leptin - physiology ; Medicine ; Medicine & Public Health ; Mice ; Mice, Inbred C57BL ; Obesity ; Obesity - complications ; Pancreas ; Pancreatic cancer ; Pancreatic Neoplasms - etiology ; Pancreatic Neoplasms - pathology ; Rodents ; Surgery</subject><ispartof>Journal of gastrointestinal surgery, 2010-12, Vol.14 (12), p.1888-1894</ispartof><rights>The Society for Surgery of the Alimentary Tract 2010</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c437t-ef48b666da6eb2dc84b967fd8733ecda1a67dfa8fff6174142a92805cc5bf0423</citedby><cites>FETCH-LOGICAL-c437t-ef48b666da6eb2dc84b967fd8733ecda1a67dfa8fff6174142a92805cc5bf0423</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11605-010-1349-x$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11605-010-1349-x$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20859700$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>White, Patrick B.</creatorcontrib><creatorcontrib>True, Eben M.</creatorcontrib><creatorcontrib>Ziegler, Kathryn M.</creatorcontrib><creatorcontrib>Wang, Sue S.</creatorcontrib><creatorcontrib>Swartz-Basile, Deborah A.</creatorcontrib><creatorcontrib>Pitt, Henry A.</creatorcontrib><creatorcontrib>Zyromski, Nicholas J.</creatorcontrib><title>Insulin, Leptin, and Tumoral Adipocytes Promote Murine Pancreatic Cancer Growth</title><title>Journal of gastrointestinal surgery</title><addtitle>J Gastrointest Surg</addtitle><addtitle>J Gastrointest Surg</addtitle><description>Background
Obesity accelerates development and growth of human pancreatic cancer. We recently reported similar findings in a novel murine model of pancreatic cancer in congenitally obese mice. The current experiments were designed to evaluate the effects of diet-induced obesity on pancreatic cancer growth.
Methods
Thirty C57BL/6J female mice were fed either control 10% fat (
n
= 10) or 60% fat diet (
n
= 20) starting at age 6 weeks. At 11 weeks, 2.5 × 10
5
PAN02 murine pancreatic cancer cells were inoculated. After 6 weeks, tumors were harvested. Serum adiponectin, leptin, insulin, and glucose concentrations were measured. Tumor proliferation, apoptosis, adipocyte content, and tumor-infiltrating lymphocytes were evaluated.
Results
The diet-induced obesity diet led to significant weight gain (control 21.3 ± 0.6 g; diet-induced obesity 23.1 ± 0.5 g;
p
= 0.03). Mice heavier than 23.1 g were considered “Overweight.” Tumors grew significantly larger in overweight (1.3 ± 0.3 g) compared to lean (0.5 ± 0.2 g;
p
= 0.03) mice; tumor size correlated positively with body weight (
R
= 0.56;
p
< 0.02). Serum leptin (3.1 ± 0.7 vs. 1.4 ± 0.2 ng/ml) and insulin (0.5 ± 0.2 vs. 0.18 ± 0.02 ng/ml) were significantly greater in overweight mice. Tumor proliferation, apoptosis, and tumor adipocyte volume were similar. T and B lymphocytes were observed infiltrating tumors from lean and overweight mice in similar number.
Conclusion
These data show that diet-induced obesity accelerates the growth of murine pancreatic cancer.</description><subject>2010 SSAT Plenary Presentation</subject><subject>Adipocytes - physiology</subject><subject>Animals</subject><subject>Cell Proliferation</subject><subject>Female</subject><subject>Gastroenterology</subject><subject>Insulin - physiology</subject><subject>Leptin - physiology</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Obesity</subject><subject>Obesity - complications</subject><subject>Pancreas</subject><subject>Pancreatic cancer</subject><subject>Pancreatic Neoplasms - etiology</subject><subject>Pancreatic Neoplasms - pathology</subject><subject>Rodents</subject><subject>Surgery</subject><issn>1091-255X</issn><issn>1873-4626</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><recordid>eNp1kE1LxDAQhoMofv8AL1Lw4MXqTJom7VEWXYWV3YOCt5CmiVbaZk1a1H9vllURwdNMyDPvDA8hRwjnCCAuAiKHPAWEFDNWpu8bZBcLkaWMU74ZeygxpXn-uEP2QngBQAFYbJMdCkVeCoBdMr_tw9g2_VkyM8thVVVfJ_dj57xqk8u6WTr9MZiQLLzr3GCSu9E3vUkWqtfeqKHRySS2xidT796G5wOyZVUbzOFX3ScP11f3k5t0Np_eTi5nqWaZGFJjWVFxzmvFTUVrXbCq5MLW8fjM6Fqh4qK2qrDWchQMGVUlLSDXOq8sMJrtk9N17tK719GEQXZN0KZtVW_cGGSBLEoQ2Yo8-UO-uNH38TiJiDTjUGYYKVxT2rsQvLFy6ZtO-Q-JIFey5Vq2hNU7ypbvceb4K3msOlP_THzbjQBdAyF-9U_G_1r9b-onJuOJ_A</recordid><startdate>20101201</startdate><enddate>20101201</enddate><creator>White, Patrick B.</creator><creator>True, Eben M.</creator><creator>Ziegler, Kathryn M.</creator><creator>Wang, Sue S.</creator><creator>Swartz-Basile, Deborah A.</creator><creator>Pitt, Henry A.</creator><creator>Zyromski, Nicholas J.</creator><general>Springer-Verlag</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RV</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>KB0</scope><scope>M0S</scope><scope>M1P</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope></search><sort><creationdate>20101201</creationdate><title>Insulin, Leptin, and Tumoral Adipocytes Promote Murine Pancreatic Cancer Growth</title><author>White, Patrick B. ; True, Eben M. ; Ziegler, Kathryn M. ; Wang, Sue S. ; Swartz-Basile, Deborah A. ; Pitt, Henry A. ; Zyromski, Nicholas J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c437t-ef48b666da6eb2dc84b967fd8733ecda1a67dfa8fff6174142a92805cc5bf0423</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>2010 SSAT Plenary Presentation</topic><topic>Adipocytes - physiology</topic><topic>Animals</topic><topic>Cell Proliferation</topic><topic>Female</topic><topic>Gastroenterology</topic><topic>Insulin - physiology</topic><topic>Leptin - physiology</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Obesity</topic><topic>Obesity - complications</topic><topic>Pancreas</topic><topic>Pancreatic cancer</topic><topic>Pancreatic Neoplasms - etiology</topic><topic>Pancreatic Neoplasms - pathology</topic><topic>Rodents</topic><topic>Surgery</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>White, Patrick B.</creatorcontrib><creatorcontrib>True, Eben M.</creatorcontrib><creatorcontrib>Ziegler, Kathryn M.</creatorcontrib><creatorcontrib>Wang, Sue S.</creatorcontrib><creatorcontrib>Swartz-Basile, Deborah A.</creatorcontrib><creatorcontrib>Pitt, Henry A.</creatorcontrib><creatorcontrib>Zyromski, Nicholas J.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Nursing & Allied Health Database</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of gastrointestinal surgery</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>White, Patrick B.</au><au>True, Eben M.</au><au>Ziegler, Kathryn M.</au><au>Wang, Sue S.</au><au>Swartz-Basile, Deborah A.</au><au>Pitt, Henry A.</au><au>Zyromski, Nicholas J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Insulin, Leptin, and Tumoral Adipocytes Promote Murine Pancreatic Cancer Growth</atitle><jtitle>Journal of gastrointestinal surgery</jtitle><stitle>J Gastrointest Surg</stitle><addtitle>J Gastrointest Surg</addtitle><date>2010-12-01</date><risdate>2010</risdate><volume>14</volume><issue>12</issue><spage>1888</spage><epage>1894</epage><pages>1888-1894</pages><issn>1091-255X</issn><eissn>1873-4626</eissn><abstract>Background
Obesity accelerates development and growth of human pancreatic cancer. We recently reported similar findings in a novel murine model of pancreatic cancer in congenitally obese mice. The current experiments were designed to evaluate the effects of diet-induced obesity on pancreatic cancer growth.
Methods
Thirty C57BL/6J female mice were fed either control 10% fat (
n
= 10) or 60% fat diet (
n
= 20) starting at age 6 weeks. At 11 weeks, 2.5 × 10
5
PAN02 murine pancreatic cancer cells were inoculated. After 6 weeks, tumors were harvested. Serum adiponectin, leptin, insulin, and glucose concentrations were measured. Tumor proliferation, apoptosis, adipocyte content, and tumor-infiltrating lymphocytes were evaluated.
Results
The diet-induced obesity diet led to significant weight gain (control 21.3 ± 0.6 g; diet-induced obesity 23.1 ± 0.5 g;
p
= 0.03). Mice heavier than 23.1 g were considered “Overweight.” Tumors grew significantly larger in overweight (1.3 ± 0.3 g) compared to lean (0.5 ± 0.2 g;
p
= 0.03) mice; tumor size correlated positively with body weight (
R
= 0.56;
p
< 0.02). Serum leptin (3.1 ± 0.7 vs. 1.4 ± 0.2 ng/ml) and insulin (0.5 ± 0.2 vs. 0.18 ± 0.02 ng/ml) were significantly greater in overweight mice. Tumor proliferation, apoptosis, and tumor adipocyte volume were similar. T and B lymphocytes were observed infiltrating tumors from lean and overweight mice in similar number.
Conclusion
These data show that diet-induced obesity accelerates the growth of murine pancreatic cancer.</abstract><cop>New York</cop><pub>Springer-Verlag</pub><pmid>20859700</pmid><doi>10.1007/s11605-010-1349-x</doi><tpages>7</tpages></addata></record> |
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| ispartof | Journal of gastrointestinal surgery, 2010-12, Vol.14 (12), p.1888-1894 |
| issn | 1091-255X 1873-4626 |
| language | eng |
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| source | MEDLINE; SpringerLink Journals - AutoHoldings |
| subjects | 2010 SSAT Plenary Presentation Adipocytes - physiology Animals Cell Proliferation Female Gastroenterology Insulin - physiology Leptin - physiology Medicine Medicine & Public Health Mice Mice, Inbred C57BL Obesity Obesity - complications Pancreas Pancreatic cancer Pancreatic Neoplasms - etiology Pancreatic Neoplasms - pathology Rodents Surgery |
| title | Insulin, Leptin, and Tumoral Adipocytes Promote Murine Pancreatic Cancer Growth |
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