A semi-mechanized method for the fluorometric determination of 5-hydroxytryptamine (serotonin) in blood plasma and platelets
1. 1. A description is given of a mechanized continuous flow system for the determination of serotonin in deproteinized plasma samples or platelet suspensions. 2. 2. The system consists of two extraction steps followed by the fluorometric assay of serotonin in the obtained final extract. 3. 3. There...
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| Veröffentlicht in: | Clinica chimica acta 1972-03, Vol.37, p.71-79 |
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| container_title | Clinica chimica acta |
| container_volume | 37 |
| creator | Hardeman, M.R. Den Uyl, A. Prins, H.K. |
| description | 1.
1. A description is given of a mechanized continuous flow system for the determination of serotonin in deproteinized plasma samples or platelet suspensions.
2.
2. The system consists of two extraction steps followed by the fluorometric assay of serotonin in the obtained final extract.
3.
3. There is no interference by related substances such as 5-hydroxytryptophan and 5-hydroxy-indole acetic acid.
4.
4. The reproducibility (standard deviation) of the method is within 3–5 ng/ml in the range of 250–750 ng added 5-hydroxytryptamine/ml plasma.
5.
5. The sensitivity allows the demonstration of less than 5 ng serotonin/ml aqueous solution. |
| doi_str_mv | 10.1016/0009-8981(72)90417-2 |
| format | Article |
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1. A description is given of a mechanized continuous flow system for the determination of serotonin in deproteinized plasma samples or platelet suspensions.
2.
2. The system consists of two extraction steps followed by the fluorometric assay of serotonin in the obtained final extract.
3.
3. There is no interference by related substances such as 5-hydroxytryptophan and 5-hydroxy-indole acetic acid.
4.
4. The reproducibility (standard deviation) of the method is within 3–5 ng/ml in the range of 250–750 ng added 5-hydroxytryptamine/ml plasma.
5.
5. The sensitivity allows the demonstration of less than 5 ng serotonin/ml aqueous solution.</description><identifier>ISSN: 0009-8981</identifier><identifier>EISSN: 1873-3492</identifier><identifier>DOI: 10.1016/0009-8981(72)90417-2</identifier><identifier>PMID: 4537106</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>5-Hydroxytryptophan ; Blood Platelets - analysis ; Chemistry, Clinical - instrumentation ; Evaluation Studies as Topic ; Humans ; Hydroxyindoleacetic Acid ; Methods ; Microchemistry ; Serotonin - blood ; Spectrometry, Fluorescence</subject><ispartof>Clinica chimica acta, 1972-03, Vol.37, p.71-79</ispartof><rights>1972</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c272t-96b18ef923d5abb936132f316318fdfd834ae5ac12d5f693cdd9c25b55dc5473</citedby><cites>FETCH-LOGICAL-c272t-96b18ef923d5abb936132f316318fdfd834ae5ac12d5f693cdd9c25b55dc5473</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0009-8981(72)90417-2$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/4537106$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hardeman, M.R.</creatorcontrib><creatorcontrib>Den Uyl, A.</creatorcontrib><creatorcontrib>Prins, H.K.</creatorcontrib><title>A semi-mechanized method for the fluorometric determination of 5-hydroxytryptamine (serotonin) in blood plasma and platelets</title><title>Clinica chimica acta</title><addtitle>Clin Chim Acta</addtitle><description>1.
1. A description is given of a mechanized continuous flow system for the determination of serotonin in deproteinized plasma samples or platelet suspensions.
2.
2. The system consists of two extraction steps followed by the fluorometric assay of serotonin in the obtained final extract.
3.
3. There is no interference by related substances such as 5-hydroxytryptophan and 5-hydroxy-indole acetic acid.
4.
4. The reproducibility (standard deviation) of the method is within 3–5 ng/ml in the range of 250–750 ng added 5-hydroxytryptamine/ml plasma.
5.
5. The sensitivity allows the demonstration of less than 5 ng serotonin/ml aqueous solution.</description><subject>5-Hydroxytryptophan</subject><subject>Blood Platelets - analysis</subject><subject>Chemistry, Clinical - instrumentation</subject><subject>Evaluation Studies as Topic</subject><subject>Humans</subject><subject>Hydroxyindoleacetic Acid</subject><subject>Methods</subject><subject>Microchemistry</subject><subject>Serotonin - blood</subject><subject>Spectrometry, Fluorescence</subject><issn>0009-8981</issn><issn>1873-3492</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1972</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1r2zAYx8XoaNNs32AFnUpy8KYXy7YuhRDablDYpXchS4-Ihi1lkjLqsg8_pwk97iTp-b886IfQF0q-UkKbb4QQWXWyo6uWrSWpaVuxD2hBu5ZXvJbsAi3eLVfoOudf87MmDb1El7XgLSXNAv3d4Ayjr0YwOx38K1g8QtlFi11MuOwAu-EQU5yHyRtsoUAafdDFx4Cjw6LaTTbFl6mkaV_0LAFeZUixxODDGvuA-yHOdftB51FjHd6uBQYo-RP66PSQ4fP5XKLnh_vn7ffq6efjj-3mqTKsZaWSTU87cJJxK3TfS95QzhynDaeds852vNYgtKHMCtdIbqyVholeCGtE3fIluj3V7lP8fYBc1OizgWHQAeIhq47WVLasmY31yWhSzDmBU_vkR50mRYk6MldHoOoIVLVMvTFXbI7dnPsP_Qj2PXSGPOt3Jx3mP_7xkFQ2HoIB6xOYomz0_1_wD5a3kwc</recordid><startdate>197203</startdate><enddate>197203</enddate><creator>Hardeman, M.R.</creator><creator>Den Uyl, A.</creator><creator>Prins, H.K.</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>197203</creationdate><title>A semi-mechanized method for the fluorometric determination of 5-hydroxytryptamine (serotonin) in blood plasma and platelets</title><author>Hardeman, M.R. ; Den Uyl, A. ; Prins, H.K.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c272t-96b18ef923d5abb936132f316318fdfd834ae5ac12d5f693cdd9c25b55dc5473</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1972</creationdate><topic>5-Hydroxytryptophan</topic><topic>Blood Platelets - analysis</topic><topic>Chemistry, Clinical - instrumentation</topic><topic>Evaluation Studies as Topic</topic><topic>Humans</topic><topic>Hydroxyindoleacetic Acid</topic><topic>Methods</topic><topic>Microchemistry</topic><topic>Serotonin - blood</topic><topic>Spectrometry, Fluorescence</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hardeman, M.R.</creatorcontrib><creatorcontrib>Den Uyl, A.</creatorcontrib><creatorcontrib>Prins, H.K.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Clinica chimica acta</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hardeman, M.R.</au><au>Den Uyl, A.</au><au>Prins, H.K.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A semi-mechanized method for the fluorometric determination of 5-hydroxytryptamine (serotonin) in blood plasma and platelets</atitle><jtitle>Clinica chimica acta</jtitle><addtitle>Clin Chim Acta</addtitle><date>1972-03</date><risdate>1972</risdate><volume>37</volume><spage>71</spage><epage>79</epage><pages>71-79</pages><issn>0009-8981</issn><eissn>1873-3492</eissn><abstract>1.
1. A description is given of a mechanized continuous flow system for the determination of serotonin in deproteinized plasma samples or platelet suspensions.
2.
2. The system consists of two extraction steps followed by the fluorometric assay of serotonin in the obtained final extract.
3.
3. There is no interference by related substances such as 5-hydroxytryptophan and 5-hydroxy-indole acetic acid.
4.
4. The reproducibility (standard deviation) of the method is within 3–5 ng/ml in the range of 250–750 ng added 5-hydroxytryptamine/ml plasma.
5.
5. The sensitivity allows the demonstration of less than 5 ng serotonin/ml aqueous solution.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>4537106</pmid><doi>10.1016/0009-8981(72)90417-2</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0009-8981 |
| ispartof | Clinica chimica acta, 1972-03, Vol.37, p.71-79 |
| issn | 0009-8981 1873-3492 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_81419726 |
| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | 5-Hydroxytryptophan Blood Platelets - analysis Chemistry, Clinical - instrumentation Evaluation Studies as Topic Humans Hydroxyindoleacetic Acid Methods Microchemistry Serotonin - blood Spectrometry, Fluorescence |
| title | A semi-mechanized method for the fluorometric determination of 5-hydroxytryptamine (serotonin) in blood plasma and platelets |
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