Studies on the state of phosphatidylcholine molecules before and after ultrasonic and gel-filtration treatments
Phosphatidylcholine vesicles, prepared by prolonged ultrasonic irradiation in solution at 2°C under nitrogen followed by chromatography at 4°C on a Sepharose 4B column, were analyzed by thin-layer chromatography. No detectable differences were found in the chemical state of phosphatidylcholine molec...
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| Veröffentlicht in: | Biochemical and biophysical research communications 1972-02, Vol.46 (4), p.1660-1666 |
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| container_end_page | 1666 |
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| container_title | Biochemical and biophysical research communications |
| container_volume | 46 |
| creator | Huang, C. Charlton, J.P. |
| description | Phosphatidylcholine vesicles, prepared by prolonged ultrasonic irradiation in solution at 2°C under nitrogen followed by chromatography at 4°C on a Sepharose 4B column, were analyzed by thin-layer chromatography. No detectable differences were found in the chemical state of phosphatidylcholine molecules before and after the treatment by ultrasonic irradiation and gel-filtration. Special emphasis is given to the differences between the procedures for the preparation of homogeneous phosphatidylcholine vesicles and the conventional ultrasonication method. |
| doi_str_mv | 10.1016/0006-291X(72)90800-5 |
| format | Article |
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No detectable differences were found in the chemical state of phosphatidylcholine molecules before and after the treatment by ultrasonic irradiation and gel-filtration. Special emphasis is given to the differences between the procedures for the preparation of homogeneous phosphatidylcholine vesicles and the conventional ultrasonication method.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1016/0006-291X(72)90800-5</identifier><identifier>PMID: 5062741</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Chromatography, Gel ; Chromatography, Thin Layer ; Egg Yolk ; Female ; Membranes, Artificial ; Methods ; Phosphatidylcholines ; Phospholipases ; Snakes ; Spectrophotometry ; Ultrasonics ; Ultraviolet Rays ; Vibration</subject><ispartof>Biochemical and biophysical research communications, 1972-02, Vol.46 (4), p.1660-1666</ispartof><rights>1972</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c423t-25a31b04260949d33292f7846e72022f698cb027e7b90de81e5d141cebf251b03</citedby><cites>FETCH-LOGICAL-c423t-25a31b04260949d33292f7846e72022f698cb027e7b90de81e5d141cebf251b03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0006-291X(72)90800-5$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/5062741$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Huang, C.</creatorcontrib><creatorcontrib>Charlton, J.P.</creatorcontrib><title>Studies on the state of phosphatidylcholine molecules before and after ultrasonic and gel-filtration treatments</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>Phosphatidylcholine vesicles, prepared by prolonged ultrasonic irradiation in solution at 2°C under nitrogen followed by chromatography at 4°C on a Sepharose 4B column, were analyzed by thin-layer chromatography. No detectable differences were found in the chemical state of phosphatidylcholine molecules before and after the treatment by ultrasonic irradiation and gel-filtration. Special emphasis is given to the differences between the procedures for the preparation of homogeneous phosphatidylcholine vesicles and the conventional ultrasonication method.</description><subject>Animals</subject><subject>Chromatography, Gel</subject><subject>Chromatography, Thin Layer</subject><subject>Egg Yolk</subject><subject>Female</subject><subject>Membranes, Artificial</subject><subject>Methods</subject><subject>Phosphatidylcholines</subject><subject>Phospholipases</subject><subject>Snakes</subject><subject>Spectrophotometry</subject><subject>Ultrasonics</subject><subject>Ultraviolet Rays</subject><subject>Vibration</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1972</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMFqFTEUhoMo9bb6BgpZSV2MPclkMpONIKVWoeCiCu5CJjnxRjKTa5IR-vbO9F66dBU45_v_cD5C3jD4wIDJKwCQDVfs52XP3ysYAJruGdkxUNBwBuI52T0hL8l5Kb8BGBNSnZGzDiTvBduRdF8XF7DQNNO6R1qqqUiTp4d9Koe9qcE9RLtPMcxIpxTRLnGlR_QpIzWzo8ZXzHSJNZuS5mAfh78wNj5ssxq25oymTjjX8oq88CYWfH16L8iPzzffr780d99uv15_umus4G1teGdaNoLgEpRQrm254r4fhMSeA-deqsGOwHvsRwUOB4adY4JZHD3v1mB7Qd4dew85_VmwVD2FYjFGM2Naih5WWkrYQHEEbU6lZPT6kMNk8oNmoDfPepOoN4m65_rRs-7W2NtT_zJO6J5CJ7Hr_uNxj-uRfwNmXWzA2aILGW3VLoX_f_APOIeN6g</recordid><startdate>19720225</startdate><enddate>19720225</enddate><creator>Huang, C.</creator><creator>Charlton, J.P.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19720225</creationdate><title>Studies on the state of phosphatidylcholine molecules before and after ultrasonic and gel-filtration treatments</title><author>Huang, C. ; Charlton, J.P.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c423t-25a31b04260949d33292f7846e72022f698cb027e7b90de81e5d141cebf251b03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1972</creationdate><topic>Animals</topic><topic>Chromatography, Gel</topic><topic>Chromatography, Thin Layer</topic><topic>Egg Yolk</topic><topic>Female</topic><topic>Membranes, Artificial</topic><topic>Methods</topic><topic>Phosphatidylcholines</topic><topic>Phospholipases</topic><topic>Snakes</topic><topic>Spectrophotometry</topic><topic>Ultrasonics</topic><topic>Ultraviolet Rays</topic><topic>Vibration</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Huang, C.</creatorcontrib><creatorcontrib>Charlton, J.P.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Huang, C.</au><au>Charlton, J.P.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Studies on the state of phosphatidylcholine molecules before and after ultrasonic and gel-filtration treatments</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>1972-02-25</date><risdate>1972</risdate><volume>46</volume><issue>4</issue><spage>1660</spage><epage>1666</epage><pages>1660-1666</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>Phosphatidylcholine vesicles, prepared by prolonged ultrasonic irradiation in solution at 2°C under nitrogen followed by chromatography at 4°C on a Sepharose 4B column, were analyzed by thin-layer chromatography. 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| fulltext | fulltext |
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| ispartof | Biochemical and biophysical research communications, 1972-02, Vol.46 (4), p.1660-1666 |
| issn | 0006-291X 1090-2104 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_81416600 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Chromatography, Gel Chromatography, Thin Layer Egg Yolk Female Membranes, Artificial Methods Phosphatidylcholines Phospholipases Snakes Spectrophotometry Ultrasonics Ultraviolet Rays Vibration |
| title | Studies on the state of phosphatidylcholine molecules before and after ultrasonic and gel-filtration treatments |
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