Biological Activity of an Encephalitogenic Fragment in the Monkey
A 45-residue encephalitogenic fragment of myelin basic protein has been prepared by extraction of acetone-ether defatted Rhesus monkey brain at pH 3.5 followed by Sephadex G-50 and carboxymethylcellulose chromatography. A ninhydrin-stained tryptic peptide map showed it to be free of contamination. A...
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| Veröffentlicht in: | The Journal of biological chemistry 1972-02, Vol.247 (3), p.969-972 |
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| container_end_page | 972 |
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| container_issue | 3 |
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| container_title | The Journal of biological chemistry |
| container_volume | 247 |
| creator | Kibler, Robert F. Re′, Peter K. McKneally, Sarah Shapira, Raymond Keeling, Michale E. |
| description | A 45-residue encephalitogenic fragment of myelin basic protein has been prepared by extraction of acetone-ether defatted Rhesus monkey brain at pH 3.5 followed by Sephadex G-50 and carboxymethylcellulose chromatography. A ninhydrin-stained tryptic peptide map showed it to be free of contamination. A dose of 1.5 mg per animal produced the classical clinical and histological changes of experimental allergic encephalomyelitis 3 to 4 weeks after challenge in three out of three Rhesus monkeys. This fragment contains the region around tyrosine 69 which is encephalitogenic in the rabbit but does not contain the other active region of the molecule. |
| doi_str_mv | 10.1016/S0021-9258(19)45702-5 |
| format | Article |
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A ninhydrin-stained tryptic peptide map showed it to be free of contamination. A dose of 1.5 mg per animal produced the classical clinical and histological changes of experimental allergic encephalomyelitis 3 to 4 weeks after challenge in three out of three Rhesus monkeys. This fragment contains the region around tyrosine 69 which is encephalitogenic in the rabbit but does not contain the other active region of the molecule.</description><identifier>ISSN: 0021-9258</identifier><identifier>EISSN: 1083-351X</identifier><identifier>DOI: 10.1016/S0021-9258(19)45702-5</identifier><identifier>PMID: 4621660</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Brain - pathology ; Chromatography, Gel ; Chromatography, Ion Exchange ; Encephalomyelitis, Autoimmune, Experimental - pathology ; Haplorhini ; Macaca ; Myelin Sheath ; Nerve Tissue Proteins - isolation & purification ; Optic Nerve - pathology ; Pons - pathology ; Species Specificity</subject><ispartof>The Journal of biological chemistry, 1972-02, Vol.247 (3), p.969-972</ispartof><rights>1972 © 1972 ASBMB. 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A ninhydrin-stained tryptic peptide map showed it to be free of contamination. A dose of 1.5 mg per animal produced the classical clinical and histological changes of experimental allergic encephalomyelitis 3 to 4 weeks after challenge in three out of three Rhesus monkeys. This fragment contains the region around tyrosine 69 which is encephalitogenic in the rabbit but does not contain the other active region of the molecule.</description><subject>Animals</subject><subject>Brain - pathology</subject><subject>Chromatography, Gel</subject><subject>Chromatography, Ion Exchange</subject><subject>Encephalomyelitis, Autoimmune, Experimental - pathology</subject><subject>Haplorhini</subject><subject>Macaca</subject><subject>Myelin Sheath</subject><subject>Nerve Tissue Proteins - isolation & purification</subject><subject>Optic Nerve - pathology</subject><subject>Pons - pathology</subject><subject>Species Specificity</subject><issn>0021-9258</issn><issn>1083-351X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1972</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkD1PwzAQhi0EKqXwEyqFBcEQ8FecZEKlagGpiAGQ2CzHuTSGJC5xWtR_j_uhrtxywz3v3elBaEjwLcFE3L1hTEmY0ii5JukNj2JMw-gI9QlOWMgi8nmM-gfkFJ0594V98ZT0UI8LSoTAfTR6MLayc6NVFYx0Z1amWwe2CFQTTBoNi1JVprNzaIwOpq2a19B0gWmCroTgxTbfsD5HJ4WqHFzs-wB9TCfv46dw9vr4PB7NQs0Z60ISKQ40izVRIuU8FRFVGVBFMBeKCpXFBRQxyzWBIsljwMpTlCkBgmdFwtkAXe32Llr7swTXydo4DVWlGrBLJxPCBE4E9WC0A3VrnWuhkIvW1KpdS4LlRp3cqpMbL5KkcqtORj433B9YZjXkh9TelZ9f7ualmZe_pgWZGatLqCXlsWQyFaln7ncMeBMrA6102oAXmXtedzK35p8v_gAiUIiC</recordid><startdate>19720210</startdate><enddate>19720210</enddate><creator>Kibler, Robert F.</creator><creator>Re′, Peter K.</creator><creator>McKneally, Sarah</creator><creator>Shapira, Raymond</creator><creator>Keeling, Michale E.</creator><general>Elsevier Inc</general><general>American Society for Biochemistry and Molecular Biology</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19720210</creationdate><title>Biological Activity of an Encephalitogenic Fragment in the Monkey</title><author>Kibler, Robert F. ; Re′, Peter K. ; McKneally, Sarah ; Shapira, Raymond ; Keeling, Michale E.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c433t-15a4e2b7c1a69449652abe2a1046a26ab7fef73dc1ef8d7e0a94423a6e64bf843</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1972</creationdate><topic>Animals</topic><topic>Brain - pathology</topic><topic>Chromatography, Gel</topic><topic>Chromatography, Ion Exchange</topic><topic>Encephalomyelitis, Autoimmune, Experimental - pathology</topic><topic>Haplorhini</topic><topic>Macaca</topic><topic>Myelin Sheath</topic><topic>Nerve Tissue Proteins - isolation & purification</topic><topic>Optic Nerve - pathology</topic><topic>Pons - pathology</topic><topic>Species Specificity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kibler, Robert F.</creatorcontrib><creatorcontrib>Re′, Peter K.</creatorcontrib><creatorcontrib>McKneally, Sarah</creatorcontrib><creatorcontrib>Shapira, Raymond</creatorcontrib><creatorcontrib>Keeling, Michale E.</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of biological chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kibler, Robert F.</au><au>Re′, Peter K.</au><au>McKneally, Sarah</au><au>Shapira, Raymond</au><au>Keeling, Michale E.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Biological Activity of an Encephalitogenic Fragment in the Monkey</atitle><jtitle>The Journal of biological chemistry</jtitle><addtitle>J Biol Chem</addtitle><date>1972-02-10</date><risdate>1972</risdate><volume>247</volume><issue>3</issue><spage>969</spage><epage>972</epage><pages>969-972</pages><issn>0021-9258</issn><eissn>1083-351X</eissn><abstract>A 45-residue encephalitogenic fragment of myelin basic protein has been prepared by extraction of acetone-ether defatted Rhesus monkey brain at pH 3.5 followed by Sephadex G-50 and carboxymethylcellulose chromatography. 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| identifier | ISSN: 0021-9258 |
| ispartof | The Journal of biological chemistry, 1972-02, Vol.247 (3), p.969-972 |
| issn | 0021-9258 1083-351X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_81360862 |
| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Animals Brain - pathology Chromatography, Gel Chromatography, Ion Exchange Encephalomyelitis, Autoimmune, Experimental - pathology Haplorhini Macaca Myelin Sheath Nerve Tissue Proteins - isolation & purification Optic Nerve - pathology Pons - pathology Species Specificity |
| title | Biological Activity of an Encephalitogenic Fragment in the Monkey |
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