Optimum concentration of ortho-phthaldialdehyde for the fluorometric measurement of serotonin
When reacted at 100°C with ortho-phthaldialdehyde (OPT), the native fluorescence of serotonin is greatly increased. For maximal serotonin fluorescence a reaction time of 5–10 min, using 0.5 g% (w/v) OPT, appears optimal over the concentration range 10–3000 ng serotonin per ml. Using these parameters...
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| Veröffentlicht in: | European journal of pharmacology 1971-10, Vol.16 (2), p.248-250 |
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| container_title | European journal of pharmacology |
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| creator | Komesu, Nathan N. Thompson, Jeremy H. |
| description | When reacted at 100°C with ortho-phthaldialdehyde (OPT), the native fluorescence of serotonin is greatly increased. For maximal serotonin fluorescence a reaction time of 5–10 min, using 0.5 g% (w/v) OPT, appears optimal over the concentration range 10–3000 ng serotonin per ml. Using these parameters, maximum separation is achieved between serotonin and blank fluorescence, and detection sensitivity and precision are highest: 1.3 ± 0.0 ng/ml and 0.38 ±0.0 ng per fluorescence intensity unit (n = 10), respectively. |
| doi_str_mv | 10.1016/0014-2999(71)90022-7 |
| format | Article |
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For maximal serotonin fluorescence a reaction time of 5–10 min, using 0.5 g% (w/v) OPT, appears optimal over the concentration range 10–3000 ng serotonin per ml. Using these parameters, maximum separation is achieved between serotonin and blank fluorescence, and detection sensitivity and precision are highest: 1.3 ± 0.0 ng/ml and 0.38 ±0.0 ng per fluorescence intensity unit (n = 10), respectively.</description><identifier>ISSN: 0014-2999</identifier><identifier>EISSN: 1879-0712</identifier><identifier>DOI: 10.1016/0014-2999(71)90022-7</identifier><identifier>PMID: 5161265</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Aldehydes ; Fluorometry ; Ortho-phthaldialdehyde ; Phthalic Acids ; Serotonin - analysis ; Serotonin-OPT ; Time Factors</subject><ispartof>European journal of pharmacology, 1971-10, Vol.16 (2), p.248-250</ispartof><rights>1971</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c357t-2aeb4fa82685bca173898291253c971edf5142808600866dd27764be70b8d56a3</citedby><cites>FETCH-LOGICAL-c357t-2aeb4fa82685bca173898291253c971edf5142808600866dd27764be70b8d56a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0014-2999(71)90022-7$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3541,27915,27916,45986</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/5161265$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Komesu, Nathan N.</creatorcontrib><creatorcontrib>Thompson, Jeremy H.</creatorcontrib><title>Optimum concentration of ortho-phthaldialdehyde for the fluorometric measurement of serotonin</title><title>European journal of pharmacology</title><addtitle>Eur J Pharmacol</addtitle><description>When reacted at 100°C with ortho-phthaldialdehyde (OPT), the native fluorescence of serotonin is greatly increased. For maximal serotonin fluorescence a reaction time of 5–10 min, using 0.5 g% (w/v) OPT, appears optimal over the concentration range 10–3000 ng serotonin per ml. Using these parameters, maximum separation is achieved between serotonin and blank fluorescence, and detection sensitivity and precision are highest: 1.3 ± 0.0 ng/ml and 0.38 ±0.0 ng per fluorescence intensity unit (n = 10), respectively.</description><subject>Aldehydes</subject><subject>Fluorometry</subject><subject>Ortho-phthaldialdehyde</subject><subject>Phthalic Acids</subject><subject>Serotonin - analysis</subject><subject>Serotonin-OPT</subject><subject>Time Factors</subject><issn>0014-2999</issn><issn>1879-0712</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1971</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kEFr3DAQhUVJSTZp_0EDPoXm4FYjW5Z0CYTQpIVALu2xCFkaYwXb2khyYf99tN0lxxyGd5j33jAfIV-AfgMK3XdKoa2ZUuqrgGtFKWO1-EA2IIWqqQB2QjZvljNyntIzpZQrxk_JKYcOWMc35O_TNvt5nSsbFotLjib7sFRhqELMY6i3Yx7N5HwZHHcOqyHEKo9FpzXEMGOO3lYzmrRGnEvBPpowhhwWv3wiHwczJfx81Avy5_7H77uf9ePTw6-728faNlzkmhns28FI1kneWwOikUoyBYw3VglAN3BomaSyo2U655gQXdujoL10vDPNBbk69G5jeFkxZT37ZHGazIJhTVpC0zJommJsD0YbQ0oRB72NfjZxp4HqPVW9R6b3yLQA_Z-qFiV2eexf-xndW-iIsexvDnssT_7zGHWyHgtQ5yParF3w7x94BVReh3A</recordid><startdate>197110</startdate><enddate>197110</enddate><creator>Komesu, Nathan N.</creator><creator>Thompson, Jeremy H.</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>197110</creationdate><title>Optimum concentration of ortho-phthaldialdehyde for the fluorometric measurement of serotonin</title><author>Komesu, Nathan N. ; Thompson, Jeremy H.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c357t-2aeb4fa82685bca173898291253c971edf5142808600866dd27764be70b8d56a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1971</creationdate><topic>Aldehydes</topic><topic>Fluorometry</topic><topic>Ortho-phthaldialdehyde</topic><topic>Phthalic Acids</topic><topic>Serotonin - analysis</topic><topic>Serotonin-OPT</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Komesu, Nathan N.</creatorcontrib><creatorcontrib>Thompson, Jeremy H.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>European journal of pharmacology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Komesu, Nathan N.</au><au>Thompson, Jeremy H.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Optimum concentration of ortho-phthaldialdehyde for the fluorometric measurement of serotonin</atitle><jtitle>European journal of pharmacology</jtitle><addtitle>Eur J Pharmacol</addtitle><date>1971-10</date><risdate>1971</risdate><volume>16</volume><issue>2</issue><spage>248</spage><epage>250</epage><pages>248-250</pages><issn>0014-2999</issn><eissn>1879-0712</eissn><abstract>When reacted at 100°C with ortho-phthaldialdehyde (OPT), the native fluorescence of serotonin is greatly increased. For maximal serotonin fluorescence a reaction time of 5–10 min, using 0.5 g% (w/v) OPT, appears optimal over the concentration range 10–3000 ng serotonin per ml. Using these parameters, maximum separation is achieved between serotonin and blank fluorescence, and detection sensitivity and precision are highest: 1.3 ± 0.0 ng/ml and 0.38 ±0.0 ng per fluorescence intensity unit (n = 10), respectively.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>5161265</pmid><doi>10.1016/0014-2999(71)90022-7</doi><tpages>3</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | European journal of pharmacology, 1971-10, Vol.16 (2), p.248-250 |
| issn | 0014-2999 1879-0712 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_81342133 |
| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Aldehydes Fluorometry Ortho-phthaldialdehyde Phthalic Acids Serotonin - analysis Serotonin-OPT Time Factors |
| title | Optimum concentration of ortho-phthaldialdehyde for the fluorometric measurement of serotonin |
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