Analysis of S-adenosylmethionine and related sulfur metabolites in animal tissues
A high-performance liquid chromatographic method was devised that separates S-adenosylmethionine and related sulfur metabolites on a Radial-PAK SCX cation-exchange column using a four-step NH 4COOH (NH 4) 2SO 4 elution gradient. This new procedure permits, in a single run of 60 min, the quantitative...
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| Veröffentlicht in: | Analytical biochemistry 1984-08, Vol.141 (1), p.161-167 |
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| creator | Miura, George A. Santangelo, James R. Gordon, Richard K. Chiang, Peter K. |
| description | A high-performance liquid chromatographic method was devised that separates
S-adenosylmethionine and related sulfur metabolites on a Radial-PAK SCX cation-exchange column using a four-step
NH
4COOH
(NH
4)
2SO
4
elution gradient. This new procedure permits, in a single run of 60 min, the quantitative analysis of
S-adenosylmethionine,
S-adenosylhomocysteine (AdoHcy), 5′-deoxy-5′-methylthioadenosine, decarboxylated
S-adenosylmethionine, decarboxylated AdoHcy, inosylhomocysteine, and other related metabolites. Furthermore, this method allows the detection in rat tissues of novel sulfur metabolites,
S-inosylhomocysteine and decarboxylated AdoHcy. Perturbation of the levels of some of these metabolites could be detected in rat livers and spleens after the administration of 3-deazaadenosine, an inhibitor of AdoHcy hydrolase, but could not be detected in rat adrenal glands. It is notable that decarboxylated AdoHcy disappeared in the livers of rats treated with 3-deazaadenosine. HeLa cells incubated with [
35S]methionine displayed the incorporation of the labeled sulfur into
S-adenosylmethionine, AdoHcy, decarboxylated
S-adenosylmethionine,
S-inosylhomocysteine, and 5′-deoxy-5′-methylthioadenosine. |
| doi_str_mv | 10.1016/0003-2697(84)90440-8 |
| format | Article |
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S-adenosylmethionine and related sulfur metabolites on a Radial-PAK SCX cation-exchange column using a four-step
NH
4COOH
(NH
4)
2SO
4
elution gradient. This new procedure permits, in a single run of 60 min, the quantitative analysis of
S-adenosylmethionine,
S-adenosylhomocysteine (AdoHcy), 5′-deoxy-5′-methylthioadenosine, decarboxylated
S-adenosylmethionine, decarboxylated AdoHcy, inosylhomocysteine, and other related metabolites. Furthermore, this method allows the detection in rat tissues of novel sulfur metabolites,
S-inosylhomocysteine and decarboxylated AdoHcy. Perturbation of the levels of some of these metabolites could be detected in rat livers and spleens after the administration of 3-deazaadenosine, an inhibitor of AdoHcy hydrolase, but could not be detected in rat adrenal glands. It is notable that decarboxylated AdoHcy disappeared in the livers of rats treated with 3-deazaadenosine. HeLa cells incubated with [
35S]methionine displayed the incorporation of the labeled sulfur into
S-adenosylmethionine, AdoHcy, decarboxylated
S-adenosylmethionine,
S-inosylhomocysteine, and 5′-deoxy-5′-methylthioadenosine.</description><identifier>ISSN: 0003-2697</identifier><identifier>EISSN: 1096-0309</identifier><identifier>DOI: 10.1016/0003-2697(84)90440-8</identifier><identifier>PMID: 6496925</identifier><identifier>CODEN: ANBCA2</identifier><language>eng</language><publisher>San Diego, CA: Elsevier Inc</publisher><subject>Adrenal Glands - analysis ; Aminoacids, peptides. Hormones. Neuropeptides ; Analytical, structural and metabolic biochemistry ; Animals ; Biological and medical sciences ; Chemical Phenomena ; Chemistry ; Chromatography, High Pressure Liquid ; decarboxylated S-adenosylhomocysteine ; decarboxylated S-adenosylmethionine ; Fishes ; Fundamental and applied biological sciences. Psychology ; HeLa Cells ; Humans ; Liver - analysis ; Male ; methylthioadenosine ; Proteins ; Rats ; Rats, Inbred Strains ; S-adenosylhomocysteine ; S-adenosylmethionine ; S-Adenosylmethionine - analysis ; S-Adenosylmethionine - metabolism ; S-inosylhomocysteine ; Spleen - analysis ; Sulfur - metabolism</subject><ispartof>Analytical biochemistry, 1984-08, Vol.141 (1), p.161-167</ispartof><rights>1984</rights><rights>1985 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c386t-a62206b0be4c2913498c6c9241adbc6aecd356d064bc65727e79581f9cc6781d3</citedby><cites>FETCH-LOGICAL-c386t-a62206b0be4c2913498c6c9241adbc6aecd356d064bc65727e79581f9cc6781d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0003-2697(84)90440-8$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=9124121$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6496925$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Miura, George A.</creatorcontrib><creatorcontrib>Santangelo, James R.</creatorcontrib><creatorcontrib>Gordon, Richard K.</creatorcontrib><creatorcontrib>Chiang, Peter K.</creatorcontrib><title>Analysis of S-adenosylmethionine and related sulfur metabolites in animal tissues</title><title>Analytical biochemistry</title><addtitle>Anal Biochem</addtitle><description>A high-performance liquid chromatographic method was devised that separates
S-adenosylmethionine and related sulfur metabolites on a Radial-PAK SCX cation-exchange column using a four-step
NH
4COOH
(NH
4)
2SO
4
elution gradient. This new procedure permits, in a single run of 60 min, the quantitative analysis of
S-adenosylmethionine,
S-adenosylhomocysteine (AdoHcy), 5′-deoxy-5′-methylthioadenosine, decarboxylated
S-adenosylmethionine, decarboxylated AdoHcy, inosylhomocysteine, and other related metabolites. Furthermore, this method allows the detection in rat tissues of novel sulfur metabolites,
S-inosylhomocysteine and decarboxylated AdoHcy. Perturbation of the levels of some of these metabolites could be detected in rat livers and spleens after the administration of 3-deazaadenosine, an inhibitor of AdoHcy hydrolase, but could not be detected in rat adrenal glands. It is notable that decarboxylated AdoHcy disappeared in the livers of rats treated with 3-deazaadenosine. HeLa cells incubated with [
35S]methionine displayed the incorporation of the labeled sulfur into
S-adenosylmethionine, AdoHcy, decarboxylated
S-adenosylmethionine,
S-inosylhomocysteine, and 5′-deoxy-5′-methylthioadenosine.</description><subject>Adrenal Glands - analysis</subject><subject>Aminoacids, peptides. Hormones. Neuropeptides</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Chemical Phenomena</subject><subject>Chemistry</subject><subject>Chromatography, High Pressure Liquid</subject><subject>decarboxylated S-adenosylhomocysteine</subject><subject>decarboxylated S-adenosylmethionine</subject><subject>Fishes</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>HeLa Cells</subject><subject>Humans</subject><subject>Liver - analysis</subject><subject>Male</subject><subject>methylthioadenosine</subject><subject>Proteins</subject><subject>Rats</subject><subject>Rats, Inbred Strains</subject><subject>S-adenosylhomocysteine</subject><subject>S-adenosylmethionine</subject><subject>S-Adenosylmethionine - analysis</subject><subject>S-Adenosylmethionine - metabolism</subject><subject>S-inosylhomocysteine</subject><subject>Spleen - analysis</subject><subject>Sulfur - metabolism</subject><issn>0003-2697</issn><issn>1096-0309</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1984</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kEtr3DAURkVJmE4m_QcteBFKsnAjybIsbQJhaB4QKKXtWsjSNVHQyImuXZh_X01nmGVWF_Gd-9Ah5DOj3xhl8ppS2tRc6u5SiStNhaC1-kCWjGpZ04bqE7I8Ih_JGeILpYyJVi7IQgotNW-X5OdtsnGLAatxqH7V1kMacRs3MD2HMYUElU2-yhDtBL7COQ5zrkpq-zGGCbAKqRBhY2M1BcQZ8JycDjYifDrUFflz9_33-qF--nH_uL59ql2j5FRbyTmVPe1BOK5ZI7Ry0mkumPW9kxacb1rpqRTl1Xa8g063ig3aOdkp5psV-bqf-5rHt7J3MpuADmK0CcYZjWINb5XqCij2oMsjYobBvOZycN4aRs3OpNlpMjtNRgnz36RRpe3LYf7cb8Afmw7qSn5xyC06G4dskwt4xDQrX-GsYDd7DIqLvwGyQRcgOfAhg5uMH8P7d_wDcziPaw</recordid><startdate>19840815</startdate><enddate>19840815</enddate><creator>Miura, George A.</creator><creator>Santangelo, James R.</creator><creator>Gordon, Richard K.</creator><creator>Chiang, Peter K.</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19840815</creationdate><title>Analysis of S-adenosylmethionine and related sulfur metabolites in animal tissues</title><author>Miura, George A. ; Santangelo, James R. ; Gordon, Richard K. ; Chiang, Peter K.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c386t-a62206b0be4c2913498c6c9241adbc6aecd356d064bc65727e79581f9cc6781d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1984</creationdate><topic>Adrenal Glands - analysis</topic><topic>Aminoacids, peptides. Hormones. Neuropeptides</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Chemical Phenomena</topic><topic>Chemistry</topic><topic>Chromatography, High Pressure Liquid</topic><topic>decarboxylated S-adenosylhomocysteine</topic><topic>decarboxylated S-adenosylmethionine</topic><topic>Fishes</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>HeLa Cells</topic><topic>Humans</topic><topic>Liver - analysis</topic><topic>Male</topic><topic>methylthioadenosine</topic><topic>Proteins</topic><topic>Rats</topic><topic>Rats, Inbred Strains</topic><topic>S-adenosylhomocysteine</topic><topic>S-adenosylmethionine</topic><topic>S-Adenosylmethionine - analysis</topic><topic>S-Adenosylmethionine - metabolism</topic><topic>S-inosylhomocysteine</topic><topic>Spleen - analysis</topic><topic>Sulfur - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Miura, George A.</creatorcontrib><creatorcontrib>Santangelo, James R.</creatorcontrib><creatorcontrib>Gordon, Richard K.</creatorcontrib><creatorcontrib>Chiang, Peter K.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Miura, George A.</au><au>Santangelo, James R.</au><au>Gordon, Richard K.</au><au>Chiang, Peter K.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Analysis of S-adenosylmethionine and related sulfur metabolites in animal tissues</atitle><jtitle>Analytical biochemistry</jtitle><addtitle>Anal Biochem</addtitle><date>1984-08-15</date><risdate>1984</risdate><volume>141</volume><issue>1</issue><spage>161</spage><epage>167</epage><pages>161-167</pages><issn>0003-2697</issn><eissn>1096-0309</eissn><coden>ANBCA2</coden><abstract>A high-performance liquid chromatographic method was devised that separates
S-adenosylmethionine and related sulfur metabolites on a Radial-PAK SCX cation-exchange column using a four-step
NH
4COOH
(NH
4)
2SO
4
elution gradient. This new procedure permits, in a single run of 60 min, the quantitative analysis of
S-adenosylmethionine,
S-adenosylhomocysteine (AdoHcy), 5′-deoxy-5′-methylthioadenosine, decarboxylated
S-adenosylmethionine, decarboxylated AdoHcy, inosylhomocysteine, and other related metabolites. Furthermore, this method allows the detection in rat tissues of novel sulfur metabolites,
S-inosylhomocysteine and decarboxylated AdoHcy. Perturbation of the levels of some of these metabolites could be detected in rat livers and spleens after the administration of 3-deazaadenosine, an inhibitor of AdoHcy hydrolase, but could not be detected in rat adrenal glands. It is notable that decarboxylated AdoHcy disappeared in the livers of rats treated with 3-deazaadenosine. HeLa cells incubated with [
35S]methionine displayed the incorporation of the labeled sulfur into
S-adenosylmethionine, AdoHcy, decarboxylated
S-adenosylmethionine,
S-inosylhomocysteine, and 5′-deoxy-5′-methylthioadenosine.</abstract><cop>San Diego, CA</cop><pub>Elsevier Inc</pub><pmid>6496925</pmid><doi>10.1016/0003-2697(84)90440-8</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Analytical biochemistry, 1984-08, Vol.141 (1), p.161-167 |
| issn | 0003-2697 1096-0309 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_81325887 |
| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Adrenal Glands - analysis Aminoacids, peptides. Hormones. Neuropeptides Analytical, structural and metabolic biochemistry Animals Biological and medical sciences Chemical Phenomena Chemistry Chromatography, High Pressure Liquid decarboxylated S-adenosylhomocysteine decarboxylated S-adenosylmethionine Fishes Fundamental and applied biological sciences. Psychology HeLa Cells Humans Liver - analysis Male methylthioadenosine Proteins Rats Rats, Inbred Strains S-adenosylhomocysteine S-adenosylmethionine S-Adenosylmethionine - analysis S-Adenosylmethionine - metabolism S-inosylhomocysteine Spleen - analysis Sulfur - metabolism |
| title | Analysis of S-adenosylmethionine and related sulfur metabolites in animal tissues |
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