[16] Actin amino-terminal acetylation and processing in a rabbit reticulocyte lysate

This chapter describes method for inhibiting the acetylation of proteins in the reticulocyte lysate. It also illustrates procedure for assaying the acetylation of the actin NH2-terminal methionine residue and the subsequent removal of acetylmethionine from the completed actin polypeptide chain. A no...

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Veröffentlicht in:	Methods in Enzymology 1984, Vol.106, p.179-192
Hauptverfasser:	Redman, Kent L., Rubenstein, Peter A.
Format:	Artikel
Sprache:	eng
Schlagworte:	Acetylation Actins - genetics Animals Coenzyme A - analogs & derivatives Coenzyme A - chemical synthesis Dictyostelium - genetics Electrophoresis, Polyacrylamide Gel - methods Indicators and Reagents Methionine - analogs & derivatives Methionine - isolation & purification Methionine - metabolism Protein Biosynthesis Protein Processing, Post-Translational Rabbits Reticulocytes - metabolism Sulfur Radioisotopes
Online-Zugang:	Volltext
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