Comparison of T and B cell analyses on fresh and aged blood
We have compared T and B cell analyses using whole blood, separated lymphocytes, and separated, frozen, then thawed lymphocytes to see how long blood can be kept before separation and analysis. We also examined the effect of various anticoagulants and the effect of diluting blood in culture media on...
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| Veröffentlicht in: | Journal of immunological methods 1984-10, Vol.73 (1), p.29-40 |
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| container_title | Journal of immunological methods |
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| creator | Nicholson, Janet K.A. Jones, Bonnie M. David Cross, G. Steven McDougal, J. |
| description | We have compared T and B cell analyses using whole blood, separated lymphocytes, and separated, frozen, then thawed lymphocytes to see how long blood can be kept before separation and analysis. We also examined the effect of various anticoagulants and the effect of diluting blood in culture media on T and B cell analysis over time. We found that the whole blood method is a very reliable method for T and B cell analysis, even 4 days after the blood is drawn, provided that heparin or ACD is the anticoagulant used. Separated lymphocytes and cryopreserved lymphocytes from blood that was separated within 24 h of collection was satisfactory; however, results were less consistent if separation was delayed more than 24 h. For lymphocyte separation, blood collected in heparin or ACD held up better over time than did blood collected in EDTA, and dilution with either RPMI 1640 or McCoy's medium gave better lymphocyte separation in older blood. |
| doi_str_mv | 10.1016/0022-1759(84)90028-0 |
| format | Article |
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We also examined the effect of various anticoagulants and the effect of diluting blood in culture media on T and B cell analysis over time. We found that the whole blood method is a very reliable method for T and B cell analysis, even 4 days after the blood is drawn, provided that heparin or ACD is the anticoagulant used. Separated lymphocytes and cryopreserved lymphocytes from blood that was separated within 24 h of collection was satisfactory; however, results were less consistent if separation was delayed more than 24 h. For lymphocyte separation, blood collected in heparin or ACD held up better over time than did blood collected in EDTA, and dilution with either RPMI 1640 or McCoy's medium gave better lymphocyte separation in older blood.</description><identifier>ISSN: 0022-1759</identifier><identifier>EISSN: 1872-7905</identifier><identifier>DOI: 10.1016/0022-1759(84)90028-0</identifier><identifier>PMID: 6333461</identifier><identifier>CODEN: JIMMBG</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Antibodies, Monoclonal - immunology ; Anticoagulants - pharmacology ; Antigens, Surface - analysis ; B-Lymphocytes - immunology ; Biological and medical sciences ; Blood Preservation ; Cell Separation ; Cell Survival ; Fixatives ; Freezing ; Fundamental and applied biological sciences. Psychology ; Fundamental immunology ; General aspects ; Humans ; Immunohematology ; Leukocyte Count ; lymphocyte storage ; lymphocyte subpopulation ; monoclonal antibody ; T and B cell analysis ; T-Lymphocytes - immunology</subject><ispartof>Journal of immunological methods, 1984-10, Vol.73 (1), p.29-40</ispartof><rights>1984</rights><rights>1985 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c301t-e2076153c27e820f3ccc83c9e63ca7ad4180a8aa18588331ae9b59c63d67a4bc3</citedby><cites>FETCH-LOGICAL-c301t-e2076153c27e820f3ccc83c9e63ca7ad4180a8aa18588331ae9b59c63d67a4bc3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0022175984900280$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=9045553$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6333461$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Nicholson, Janet K.A.</creatorcontrib><creatorcontrib>Jones, Bonnie M.</creatorcontrib><creatorcontrib>David Cross, G.</creatorcontrib><creatorcontrib>Steven McDougal, J.</creatorcontrib><title>Comparison of T and B cell analyses on fresh and aged blood</title><title>Journal of immunological methods</title><addtitle>J Immunol Methods</addtitle><description>We have compared T and B cell analyses using whole blood, separated lymphocytes, and separated, frozen, then thawed lymphocytes to see how long blood can be kept before separation and analysis. We also examined the effect of various anticoagulants and the effect of diluting blood in culture media on T and B cell analysis over time. We found that the whole blood method is a very reliable method for T and B cell analysis, even 4 days after the blood is drawn, provided that heparin or ACD is the anticoagulant used. Separated lymphocytes and cryopreserved lymphocytes from blood that was separated within 24 h of collection was satisfactory; however, results were less consistent if separation was delayed more than 24 h. For lymphocyte separation, blood collected in heparin or ACD held up better over time than did blood collected in EDTA, and dilution with either RPMI 1640 or McCoy's medium gave better lymphocyte separation in older blood.</description><subject>Antibodies, Monoclonal - immunology</subject><subject>Anticoagulants - pharmacology</subject><subject>Antigens, Surface - analysis</subject><subject>B-Lymphocytes - immunology</subject><subject>Biological and medical sciences</subject><subject>Blood Preservation</subject><subject>Cell Separation</subject><subject>Cell Survival</subject><subject>Fixatives</subject><subject>Freezing</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fundamental immunology</subject><subject>General aspects</subject><subject>Humans</subject><subject>Immunohematology</subject><subject>Leukocyte Count</subject><subject>lymphocyte storage</subject><subject>lymphocyte subpopulation</subject><subject>monoclonal antibody</subject><subject>T and B cell analysis</subject><subject>T-Lymphocytes - immunology</subject><issn>0022-1759</issn><issn>1872-7905</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1984</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1Lw0AQhhdRav34Bwo5iOghOpvNJrsIgha_oOClnpfJZqKRNFt3W6H_3qQNPXqaGd5nhuFh7IzDDQee3QIkScxzqa9Ueq27ScWwx8Zc5Umca5D7bLxDDtlRCN8AwCGDERtlQog042N2N3HzBfo6uDZyVTSLsC2jx8hS03QtNutAIeqyylP42oT4SWVUNM6VJ-ygwibQ6VCP2cfz02zyGk_fX94mD9PYCuDLmBLIMy6FTXJSCVTCWquE1ZQJizmWKVeACpErqZQQHEkXUttMlFmOaWHFMbvc3l1497OisDTzOvQfYktuFYzigoPWsgPTLWi9C8FTZRa-nqNfGw6md2Z6IaYXYlRqNs4MdGvnw_1VMadytzRI6vKLIcdgsak8trYOO0xDKqUUHXa_xahz8VuTN8HW1Foqa092aUpX___HH1YVhTM</recordid><startdate>19841012</startdate><enddate>19841012</enddate><creator>Nicholson, Janet K.A.</creator><creator>Jones, Bonnie M.</creator><creator>David Cross, G.</creator><creator>Steven McDougal, J.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19841012</creationdate><title>Comparison of T and B cell analyses on fresh and aged blood</title><author>Nicholson, Janet K.A. ; Jones, Bonnie M. ; David Cross, G. ; Steven McDougal, J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c301t-e2076153c27e820f3ccc83c9e63ca7ad4180a8aa18588331ae9b59c63d67a4bc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1984</creationdate><topic>Antibodies, Monoclonal - immunology</topic><topic>Anticoagulants - pharmacology</topic><topic>Antigens, Surface - analysis</topic><topic>B-Lymphocytes - immunology</topic><topic>Biological and medical sciences</topic><topic>Blood Preservation</topic><topic>Cell Separation</topic><topic>Cell Survival</topic><topic>Fixatives</topic><topic>Freezing</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fundamental immunology</topic><topic>General aspects</topic><topic>Humans</topic><topic>Immunohematology</topic><topic>Leukocyte Count</topic><topic>lymphocyte storage</topic><topic>lymphocyte subpopulation</topic><topic>monoclonal antibody</topic><topic>T and B cell analysis</topic><topic>T-Lymphocytes - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nicholson, Janet K.A.</creatorcontrib><creatorcontrib>Jones, Bonnie M.</creatorcontrib><creatorcontrib>David Cross, G.</creatorcontrib><creatorcontrib>Steven McDougal, J.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of immunological methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nicholson, Janet K.A.</au><au>Jones, Bonnie M.</au><au>David Cross, G.</au><au>Steven McDougal, J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparison of T and B cell analyses on fresh and aged blood</atitle><jtitle>Journal of immunological methods</jtitle><addtitle>J Immunol Methods</addtitle><date>1984-10-12</date><risdate>1984</risdate><volume>73</volume><issue>1</issue><spage>29</spage><epage>40</epage><pages>29-40</pages><issn>0022-1759</issn><eissn>1872-7905</eissn><coden>JIMMBG</coden><abstract>We have compared T and B cell analyses using whole blood, separated lymphocytes, and separated, frozen, then thawed lymphocytes to see how long blood can be kept before separation and analysis. We also examined the effect of various anticoagulants and the effect of diluting blood in culture media on T and B cell analysis over time. We found that the whole blood method is a very reliable method for T and B cell analysis, even 4 days after the blood is drawn, provided that heparin or ACD is the anticoagulant used. Separated lymphocytes and cryopreserved lymphocytes from blood that was separated within 24 h of collection was satisfactory; however, results were less consistent if separation was delayed more than 24 h. For lymphocyte separation, blood collected in heparin or ACD held up better over time than did blood collected in EDTA, and dilution with either RPMI 1640 or McCoy's medium gave better lymphocyte separation in older blood.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>6333461</pmid><doi>10.1016/0022-1759(84)90028-0</doi><tpages>12</tpages></addata></record> |
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| identifier | ISSN: 0022-1759 |
| ispartof | Journal of immunological methods, 1984-10, Vol.73 (1), p.29-40 |
| issn | 0022-1759 1872-7905 |
| language | eng |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Antibodies, Monoclonal - immunology Anticoagulants - pharmacology Antigens, Surface - analysis B-Lymphocytes - immunology Biological and medical sciences Blood Preservation Cell Separation Cell Survival Fixatives Freezing Fundamental and applied biological sciences. Psychology Fundamental immunology General aspects Humans Immunohematology Leukocyte Count lymphocyte storage lymphocyte subpopulation monoclonal antibody T and B cell analysis T-Lymphocytes - immunology |
| title | Comparison of T and B cell analyses on fresh and aged blood |
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